Search results for the GEO ID: GSE11394  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM287679 | GPL1352 |
|
Bipotent-enriched Biological Rep1
|
Bipotent progenitor Enriched subpopulation
|
Human reduction mammoplasty sample, 25 year old patient
|
Gene expression data from bipotent progenitor-enriched subpopulation of mammary epithelial cells
|
| Sample_geo_accession | GSM287679
| | Sample_status | Public on May 10 2008
| | Sample_submission_date | May 09 2008
| | Sample_last_update_date | May 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Epithelial-enriched fraction of reduction mammoplasty samples were made into single cell suspenssions and co-culutred with irratdiated mouse fibroblasts for 3 days
| | Sample_growth_protocol_ch1 | Primary cells extracted from reduction mammoplasty samples were cultured in EpiCult-B medium (StemCell Technologies) with 5% fetal Calf Serum supplementation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 50 pg aliquotsof RNA were PCR amplified (Iscove et al., 2002) from each sample yielding 2-3 µg of amplified product. The amplified cDNA preparations were then purified using the High Pure PCR Product purification kit (Roche Applied Science), end-labelled with Biotin-N6 ddATP (StemCore Laboratories, OHRI, Ottawa, Ont., Canada)
| | Sample_hyb_protocol | cDNA probes were hybridized to Affymetrix human X3P GeneChip arrays according to the manufacturer’s protocol (Tietjen et al., 2003)
| | Sample_scan_protocol | GeneChip® Scanner 3000 7G, Affymetrix
| | Sample_data_processing | The data were analyzed with ArrayAssist software (http://Stratagene.com/software solutions) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | BCGSC,,BC Cancer Agency
| | Sample_contact_email | support@mouseatlas.org
| | Sample_contact_laboratory | Marco Marra
| | Sample_contact_department | Gene Expression
| | Sample_contact_institute | Canada's Michael Smith Genome Sciences Centre
| | Sample_contact_address | 675 West 10th Avenue
| | Sample_contact_city | Vancouver
| | Sample_contact_state | BC
| | Sample_contact_zip/postal_code | V5Z 1L3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM287nnn/GSM287679/suppl/GSM287679.CEL.gz
| | Sample_series_id | GSE11394
| | Sample_series_id | GSE11395
| | Sample_data_row_count | 61359
| | |
|
GSM287680 | GPL1352 |
|
Bipotent-enriched Biological Rep2
|
Bipotent progenitor Enriched subpopulation
|
Human reduction mammoplasty sample, 26 year old patient
|
Gene expression data from bipotent progenitor-enriched subpopulation of mammary epithelial cells
|
| Sample_geo_accession | GSM287680
| | Sample_status | Public on May 10 2008
| | Sample_submission_date | May 09 2008
| | Sample_last_update_date | May 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Epithelial-enriched fraction of reduction mammoplasty samples were made into single cell suspenssions and co-culutred with irratdiated mouse fibroblasts for 3 days
| | Sample_growth_protocol_ch1 | Primary cells extracted from reduction mammoplasty samples were cultured in EpiCult-B medium (StemCell Technologies) with 5% fetal Calf Serum supplementation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 50 pg aliquotsof RNA were PCR amplified (Iscove et al., 2002) from each sample yielding 2-3 µg of amplified product. The amplified cDNA preparations were then purified using the High Pure PCR Product purification kit (Roche Applied Science), end-labelled with Biotin-N6 ddATP (StemCore Laboratories, OHRI, Ottawa, Ont., Canada)
| | Sample_hyb_protocol | cDNA probes were hybridized to Affymetrix human X3P GeneChip arrays according to the manufacturer’s protocol (Tietjen et al., 2003)
| | Sample_scan_protocol | GeneChip® Scanner 3000 7G, Affymetrix
| | Sample_data_processing | The data were analyzed with ArrayAssist software (http://Stratagene.com/software solutions) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | BCGSC,,BC Cancer Agency
| | Sample_contact_email | support@mouseatlas.org
| | Sample_contact_laboratory | Marco Marra
| | Sample_contact_department | Gene Expression
| | Sample_contact_institute | Canada's Michael Smith Genome Sciences Centre
| | Sample_contact_address | 675 West 10th Avenue
| | Sample_contact_city | Vancouver
| | Sample_contact_state | BC
| | Sample_contact_zip/postal_code | V5Z 1L3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM287nnn/GSM287680/suppl/GSM287680.CEL.gz
| | Sample_series_id | GSE11394
| | Sample_series_id | GSE11395
| | Sample_data_row_count | 61359
| | |
|
GSM287681 | GPL1352 |
|
Bipotent-enriched Biological Rep3
|
Bipotent progenitor Enriched subpopulation
|
Human reduction mammoplasty sample, 55 year old patient
|
Gene expression data from bipotent progenitor-enriched subpopulation of mammary epithelial cells
|
| Sample_geo_accession | GSM287681
| | Sample_status | Public on May 10 2008
| | Sample_submission_date | May 09 2008
| | Sample_last_update_date | May 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Epithelial-enriched fraction of reduction mammoplasty samples were made into single cell suspenssions and co-culutred with irratdiated mouse fibroblasts for 3 days
| | Sample_growth_protocol_ch1 | Primary cells extracted from reduction mammoplasty samples were cultured in EpiCult-B medium (StemCell Technologies) with 5% fetal Calf Serum supplementation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 50 pg aliquotsof RNA were PCR amplified (Iscove et al., 2002) from each sample yielding 2-3 µg of amplified product. The amplified cDNA preparations were then purified using the High Pure PCR Product purification kit (Roche Applied Science), end-labelled with Biotin-N6 ddATP (StemCore Laboratories, OHRI, Ottawa, Ont., Canada)
| | Sample_hyb_protocol | cDNA probes were hybridized to Affymetrix human X3P GeneChip arrays according to the manufacturer’s protocol (Tietjen et al., 2003)
| | Sample_scan_protocol | GeneChip® Scanner 3000 7G, Affymetrix
| | Sample_data_processing | The data were analyzed with ArrayAssist software (http://Stratagene.com/software solutions) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | BCGSC,,BC Cancer Agency
| | Sample_contact_email | support@mouseatlas.org
| | Sample_contact_laboratory | Marco Marra
| | Sample_contact_department | Gene Expression
| | Sample_contact_institute | Canada's Michael Smith Genome Sciences Centre
| | Sample_contact_address | 675 West 10th Avenue
| | Sample_contact_city | Vancouver
| | Sample_contact_state | BC
| | Sample_contact_zip/postal_code | V5Z 1L3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM287nnn/GSM287681/suppl/GSM287681.CEL.gz
| | Sample_series_id | GSE11394
| | Sample_series_id | GSE11395
| | Sample_data_row_count | 61359
| | |
|
GSM287682 | GPL1352 |
|
Committed Luminal progentiors Biological Rep1
|
Committed luminal progenitor-enriched subpopulation
|
Human reduction mammoplasty sample, 25 year old patient
|
Gene expression data from committed luminal progenitor-enriched subpopulation of mammary epithelial cells
|
| Sample_geo_accession | GSM287682
| | Sample_status | Public on May 10 2008
| | Sample_submission_date | May 09 2008
| | Sample_last_update_date | May 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Epithelial-enriched fraction of reduction mammoplasty samples were made into single cell suspenssions and co-culutred with irratdiated mouse fibroblasts for 3 days
| | Sample_growth_protocol_ch1 | Primary cells extracted from reduction mammoplasty samples were cultured in EpiCult-B medium (StemCell Technologies) with 5% fetal Calf Serum supplementation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 50 pg aliquotsof RNA were PCR amplified (Iscove et al., 2002) from each sample yielding 2-3 µg of amplified product. The amplified cDNA preparations were then purified using the High Pure PCR Product purification kit (Roche Applied Science), end-labelled with Biotin-N6 ddATP (StemCore Laboratories, OHRI, Ottawa, Ont., Canada)
| | Sample_hyb_protocol | cDNA probes were hybridized to Affymetrix human X3P GeneChip arrays according to the manufacturer’s protocol (Tietjen et al., 2003)
| | Sample_scan_protocol | GeneChip® Scanner 3000 7G, Affymetrix
| | Sample_data_processing | The data were analyzed with ArrayAssist software (http://Stratagene.com/software solutions) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | BCGSC,,BC Cancer Agency
| | Sample_contact_email | support@mouseatlas.org
| | Sample_contact_laboratory | Marco Marra
| | Sample_contact_department | Gene Expression
| | Sample_contact_institute | Canada's Michael Smith Genome Sciences Centre
| | Sample_contact_address | 675 West 10th Avenue
| | Sample_contact_city | Vancouver
| | Sample_contact_state | BC
| | Sample_contact_zip/postal_code | V5Z 1L3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM287nnn/GSM287682/suppl/GSM287682.CEL.gz
| | Sample_series_id | GSE11394
| | Sample_series_id | GSE11395
| | Sample_data_row_count | 61359
| | |
|
GSM287683 | GPL1352 |
|
Committed Luminal progentiorsBiological Rep2
|
Committed luminal progenitor-enriched subpopulation
|
Human reduction mammoplasty sample, 26 year old patient
|
Gene expression data from committed luminal progenitor-enriched subpopulation of mammary epithelial cells
|
| Sample_geo_accession | GSM287683
| | Sample_status | Public on May 10 2008
| | Sample_submission_date | May 09 2008
| | Sample_last_update_date | May 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Epithelial-enriched fraction of reduction mammoplasty samples were made into single cell suspenssions and co-culutred with irratdiated mouse fibroblasts for 3 days
| | Sample_growth_protocol_ch1 | Primary cells extracted from reduction mammoplasty samples were cultured in EpiCult-B medium (StemCell Technologies) with 5% fetal Calf Serum supplementation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 50 pg aliquotsof RNA were PCR amplified (Iscove et al., 2002) from each sample yielding 2-3 µg of amplified product. The amplified cDNA preparations were then purified using the High Pure PCR Product purification kit (Roche Applied Science), end-labelled with Biotin-N6 ddATP (StemCore Laboratories, OHRI, Ottawa, Ont., Canada)
| | Sample_hyb_protocol | cDNA probes were hybridized to Affymetrix human X3P GeneChip arrays according to the manufacturer’s protocol (Tietjen et al., 2003)
| | Sample_scan_protocol | GeneChip® Scanner 3000 7G, Affymetrix
| | Sample_data_processing | The data were analyzed with ArrayAssist software (http://Stratagene.com/software solutions) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | BCGSC,,BC Cancer Agency
| | Sample_contact_email | support@mouseatlas.org
| | Sample_contact_laboratory | Marco Marra
| | Sample_contact_department | Gene Expression
| | Sample_contact_institute | Canada's Michael Smith Genome Sciences Centre
| | Sample_contact_address | 675 West 10th Avenue
| | Sample_contact_city | Vancouver
| | Sample_contact_state | BC
| | Sample_contact_zip/postal_code | V5Z 1L3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM287nnn/GSM287683/suppl/GSM287683.CEL.gz
| | Sample_series_id | GSE11394
| | Sample_series_id | GSE11395
| | Sample_data_row_count | 61359
| | |
|
GSM287684 | GPL1352 |
|
Committed Luminal progentiors Biological Rep3
|
Committed luminal progenitor-enriched subpopulation
|
Human reduction mammoplasty sample, 55 year old patient
|
Gene expression data from committed luminal progenitor-enriched subpopulation of mammary epithelial cells
|
| Sample_geo_accession | GSM287684
| | Sample_status | Public on May 10 2008
| | Sample_submission_date | May 09 2008
| | Sample_last_update_date | May 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Epithelial-enriched fraction of reduction mammoplasty samples were made into single cell suspenssions and co-culutred with irratdiated mouse fibroblasts for 3 days
| | Sample_growth_protocol_ch1 | Primary cells extracted from reduction mammoplasty samples were cultured in EpiCult-B medium (StemCell Technologies) with 5% fetal Calf Serum supplementation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 50 pg aliquotsof RNA were PCR amplified (Iscove et al., 2002) from each sample yielding 2-3 µg of amplified product. The amplified cDNA preparations were then purified using the High Pure PCR Product purification kit (Roche Applied Science), end-labelled with Biotin-N6 ddATP (StemCore Laboratories, OHRI, Ottawa, Ont., Canada)
| | Sample_hyb_protocol | cDNA probes were hybridized to Affymetrix human X3P GeneChip arrays according to the manufacturer’s protocol (Tietjen et al., 2003)
| | Sample_scan_protocol | GeneChip® Scanner 3000 7G, Affymetrix
| | Sample_data_processing | The data were analyzed with ArrayAssist software (http://Stratagene.com/software solutions) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | BCGSC,,BC Cancer Agency
| | Sample_contact_email | support@mouseatlas.org
| | Sample_contact_laboratory | Marco Marra
| | Sample_contact_department | Gene Expression
| | Sample_contact_institute | Canada's Michael Smith Genome Sciences Centre
| | Sample_contact_address | 675 West 10th Avenue
| | Sample_contact_city | Vancouver
| | Sample_contact_state | BC
| | Sample_contact_zip/postal_code | V5Z 1L3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM287nnn/GSM287684/suppl/GSM287684.CEL.gz
| | Sample_series_id | GSE11394
| | Sample_series_id | GSE11395
| | Sample_data_row_count | 61359
| | |
|
GSM287685 | GPL1352 |
|
Differentiated Basal Biological Rep1
|
Differentiated basal Cells
|
Human reduction mammoplasty sample, 25 year old patient
|
Gene expression data from differentiated basal subpopulation of mammary epithelial cells
|
| Sample_geo_accession | GSM287685
| | Sample_status | Public on May 10 2008
| | Sample_submission_date | May 09 2008
| | Sample_last_update_date | May 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Epithelial-enriched fraction of reduction mammoplasty samples were made into single cell suspenssions and co-culutred with irratdiated mouse fibroblasts for 3 days
| | Sample_growth_protocol_ch1 | Primary cells extracted from reduction mammoplasty samples were cultured in EpiCult-B medium (StemCell Technologies) with 5% fetal Calf Serum supplementation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 50 pg aliquotsof RNA were PCR amplified (Iscove et al., 2002) from each sample yielding 2-3 µg of amplified product. The amplified cDNA preparations were then purified using the High Pure PCR Product purification kit (Roche Applied Science), end-labelled with Biotin-N6 ddATP (StemCore Laboratories, OHRI, Ottawa, Ont., Canada)
| | Sample_hyb_protocol | cDNA probes were hybridized to Affymetrix human X3P GeneChip arrays according to the manufacturer’s protocol (Tietjen et al., 2003)
| | Sample_scan_protocol | GeneChip® Scanner 3000 7G, Affymetrix
| | Sample_data_processing | The data were analyzed with ArrayAssist software (http://Stratagene.com/software solutions) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | BCGSC,,BC Cancer Agency
| | Sample_contact_email | support@mouseatlas.org
| | Sample_contact_laboratory | Marco Marra
| | Sample_contact_department | Gene Expression
| | Sample_contact_institute | Canada's Michael Smith Genome Sciences Centre
| | Sample_contact_address | 675 West 10th Avenue
| | Sample_contact_city | Vancouver
| | Sample_contact_state | BC
| | Sample_contact_zip/postal_code | V5Z 1L3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM287nnn/GSM287685/suppl/GSM287685.CEL.gz
| | Sample_series_id | GSE11394
| | Sample_series_id | GSE11395
| | Sample_data_row_count | 61359
| | |
|
GSM287686 | GPL1352 |
|
Differentiated basal Biological Rep2
|
Differentiated basal Cells
|
Human reduction mammoplasty sample, 26 year old patient
|
Gene expression data from differentiated basal subpopulation of mammary epithelial cells
|
| Sample_geo_accession | GSM287686
| | Sample_status | Public on May 10 2008
| | Sample_submission_date | May 09 2008
| | Sample_last_update_date | May 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Epithelial-enriched fraction of reduction mammoplasty samples were made into single cell suspenssions and co-culutred with irratdiated mouse fibroblasts for 3 days
| | Sample_growth_protocol_ch1 | Primary cells extracted from reduction mammoplasty samples were cultured in EpiCult-B medium (StemCell Technologies) with 5% fetal Calf Serum supplementation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 50 pg aliquotsof RNA were PCR amplified (Iscove et al., 2002) from each sample yielding 2-3 µg of amplified product. The amplified cDNA preparations were then purified using the High Pure PCR Product purification kit (Roche Applied Science), end-labelled with Biotin-N6 ddATP (StemCore Laboratories, OHRI, Ottawa, Ont., Canada)
| | Sample_hyb_protocol | cDNA probes were hybridized to Affymetrix human X3P GeneChip arrays according to the manufacturer’s protocol (Tietjen et al., 2003)
| | Sample_scan_protocol | GeneChip® Scanner 3000 7G, Affymetrix
| | Sample_data_processing | The data were analyzed with ArrayAssist software (http://Stratagene.com/software solutions) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | BCGSC,,BC Cancer Agency
| | Sample_contact_email | support@mouseatlas.org
| | Sample_contact_laboratory | Marco Marra
| | Sample_contact_department | Gene Expression
| | Sample_contact_institute | Canada's Michael Smith Genome Sciences Centre
| | Sample_contact_address | 675 West 10th Avenue
| | Sample_contact_city | Vancouver
| | Sample_contact_state | BC
| | Sample_contact_zip/postal_code | V5Z 1L3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM287nnn/GSM287686/suppl/GSM287686.CEL.gz
| | Sample_series_id | GSE11394
| | Sample_series_id | GSE11395
| | Sample_data_row_count | 61359
| | |
|
GSM287687 | GPL1352 |
|
Differentiated basal Biological Rep3
|
Differentiated basal Cells
|
Human reduction mammoplasty sample, 55 year old patient
|
Gene expression data from differentiated basal subpopulation of mammary epithelial cells
|
| Sample_geo_accession | GSM287687
| | Sample_status | Public on May 10 2008
| | Sample_submission_date | May 09 2008
| | Sample_last_update_date | May 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Epithelial-enriched fraction of reduction mammoplasty samples were made into single cell suspenssions and co-culutred with irratdiated mouse fibroblasts for 3 days
| | Sample_growth_protocol_ch1 | Primary cells extracted from reduction mammoplasty samples were cultured in EpiCult-B medium (StemCell Technologies) with 5% fetal Calf Serum supplementation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 50 pg aliquotsof RNA were PCR amplified (Iscove et al., 2002) from each sample yielding 2-3 µg of amplified product. The amplified cDNA preparations were then purified using the High Pure PCR Product purification kit (Roche Applied Science), end-labelled with Biotin-N6 ddATP (StemCore Laboratories, OHRI, Ottawa, Ont., Canada)
| | Sample_hyb_protocol | cDNA probes were hybridized to Affymetrix human X3P GeneChip arrays according to the manufacturer’s protocol (Tietjen et al., 2003)
| | Sample_scan_protocol | GeneChip® Scanner 3000 7G, Affymetrix
| | Sample_data_processing | The data were analyzed with ArrayAssist software (http://Stratagene.com/software solutions) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | BCGSC,,BC Cancer Agency
| | Sample_contact_email | support@mouseatlas.org
| | Sample_contact_laboratory | Marco Marra
| | Sample_contact_department | Gene Expression
| | Sample_contact_institute | Canada's Michael Smith Genome Sciences Centre
| | Sample_contact_address | 675 West 10th Avenue
| | Sample_contact_city | Vancouver
| | Sample_contact_state | BC
| | Sample_contact_zip/postal_code | V5Z 1L3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM287nnn/GSM287687/suppl/GSM287687.CEL.gz
| | Sample_series_id | GSE11394
| | Sample_series_id | GSE11395
| | Sample_data_row_count | 61359
| | |
|
GSM287688 | GPL1352 |
|
Differentiated Luminal Biological Rep1
|
Differentiated luminal Cells
|
Human reduction mammoplasty sample, 25 year old patient
|
Gene expression data from differentiated luminal subpopulation of mammary epithelial cells
|
| Sample_geo_accession | GSM287688
| | Sample_status | Public on May 10 2008
| | Sample_submission_date | May 09 2008
| | Sample_last_update_date | May 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Epithelial-enriched fraction of reduction mammoplasty samples were made into single cell suspenssions and co-culutred with irratdiated mouse fibroblasts for 3 days
| | Sample_growth_protocol_ch1 | Primary cells extracted from reduction mammoplasty samples were cultured in EpiCult-B medium (StemCell Technologies) with 5% fetal Calf Serum supplementation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 50 pg aliquotsof RNA were PCR amplified (Iscove et al., 2002) from each sample yielding 2-3 µg of amplified product. The amplified cDNA preparations were then purified using the High Pure PCR Product purification kit (Roche Applied Science), end-labelled with Biotin-N6 ddATP (StemCore Laboratories, OHRI, Ottawa, Ont., Canada)
| | Sample_hyb_protocol | cDNA probes were hybridized to Affymetrix human X3P GeneChip arrays according to the manufacturer’s protocol (Tietjen et al., 2003)
| | Sample_scan_protocol | GeneChip® Scanner 3000 7G, Affymetrix
| | Sample_data_processing | The data were analyzed with ArrayAssist software (http://Stratagene.com/software solutions) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | BCGSC,,BC Cancer Agency
| | Sample_contact_email | support@mouseatlas.org
| | Sample_contact_laboratory | Marco Marra
| | Sample_contact_department | Gene Expression
| | Sample_contact_institute | Canada's Michael Smith Genome Sciences Centre
| | Sample_contact_address | 675 West 10th Avenue
| | Sample_contact_city | Vancouver
| | Sample_contact_state | BC
| | Sample_contact_zip/postal_code | V5Z 1L3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM287nnn/GSM287688/suppl/GSM287688.CEL.gz
| | Sample_series_id | GSE11394
| | Sample_series_id | GSE11395
| | Sample_data_row_count | 61359
| | |
|
GSM287689 | GPL1352 |
|
Differentiated Luminal Biological Rep2
|
Differentiated luminal Cells
|
Human reduction mammoplasty sample, 26 year old patient
|
Gene expression data from differentiated luminal subpopulation of mammary epithelial cells
|
| Sample_geo_accession | GSM287689
| | Sample_status | Public on May 10 2008
| | Sample_submission_date | May 09 2008
| | Sample_last_update_date | May 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Epithelial-enriched fraction of reduction mammoplasty samples were made into single cell suspenssions and co-culutred with irratdiated mouse fibroblasts for 3 days
| | Sample_growth_protocol_ch1 | Primary cells extracted from reduction mammoplasty samples were cultured in EpiCult-B medium (StemCell Technologies) with 5% fetal Calf Serum supplementation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 50 pg aliquotsof RNA were PCR amplified (Iscove et al., 2002) from each sample yielding 2-3 µg of amplified product. The amplified cDNA preparations were then purified using the High Pure PCR Product purification kit (Roche Applied Science), end-labelled with Biotin-N6 ddATP (StemCore Laboratories, OHRI, Ottawa, Ont., Canada)
| | Sample_hyb_protocol | cDNA probes were hybridized to Affymetrix human X3P GeneChip arrays according to the manufacturer’s protocol (Tietjen et al., 2003)
| | Sample_scan_protocol | GeneChip® Scanner 3000 7G, Affymetrix
| | Sample_data_processing | The data were analyzed with ArrayAssist software (http://Stratagene.com/software solutions) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | BCGSC,,BC Cancer Agency
| | Sample_contact_email | support@mouseatlas.org
| | Sample_contact_laboratory | Marco Marra
| | Sample_contact_department | Gene Expression
| | Sample_contact_institute | Canada's Michael Smith Genome Sciences Centre
| | Sample_contact_address | 675 West 10th Avenue
| | Sample_contact_city | Vancouver
| | Sample_contact_state | BC
| | Sample_contact_zip/postal_code | V5Z 1L3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM287nnn/GSM287689/suppl/GSM287689.CEL.gz
| | Sample_series_id | GSE11394
| | Sample_series_id | GSE11395
| | Sample_data_row_count | 61359
| | |
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GSM287690 | GPL1352 |
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Differentiated Luminal Biological Rep3
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Differentiated luminal Cells
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Human reduction mammoplasty sample, 55 year old patient
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Gene expression data from differentiated luminal subpopulation of mammary epithelial cells
|
| Sample_geo_accession | GSM287690
| | Sample_status | Public on May 10 2008
| | Sample_submission_date | May 09 2008
| | Sample_last_update_date | May 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Epithelial-enriched fraction of reduction mammoplasty samples were made into single cell suspenssions and co-culutred with irratdiated mouse fibroblasts for 3 days
| | Sample_growth_protocol_ch1 | Primary cells extracted from reduction mammoplasty samples were cultured in EpiCult-B medium (StemCell Technologies) with 5% fetal Calf Serum supplementation.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and DNase treated.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 50 pg aliquotsof RNA were PCR amplified (Iscove et al., 2002) from each sample yielding 2-3 µg of amplified product. The amplified cDNA preparations were then purified using the High Pure PCR Product purification kit (Roche Applied Science), end-labelled with Biotin-N6 ddATP (StemCore Laboratories, OHRI, Ottawa, Ont., Canada)
| | Sample_hyb_protocol | cDNA probes were hybridized to Affymetrix human X3P GeneChip arrays according to the manufacturer’s protocol (Tietjen et al., 2003)
| | Sample_scan_protocol | GeneChip® Scanner 3000 7G, Affymetrix
| | Sample_data_processing | The data were analyzed with ArrayAssist software (http://Stratagene.com/software solutions) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1352
| | Sample_contact_name | BCGSC,,BC Cancer Agency
| | Sample_contact_email | support@mouseatlas.org
| | Sample_contact_laboratory | Marco Marra
| | Sample_contact_department | Gene Expression
| | Sample_contact_institute | Canada's Michael Smith Genome Sciences Centre
| | Sample_contact_address | 675 West 10th Avenue
| | Sample_contact_city | Vancouver
| | Sample_contact_state | BC
| | Sample_contact_zip/postal_code | V5Z 1L3
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM287nnn/GSM287690/suppl/GSM287690.CEL.gz
| | Sample_series_id | GSE11394
| | Sample_series_id | GSE11395
| | Sample_data_row_count | 61359
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