Search results for the GEO ID: GSE11446  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM288809 | GPL1261 |
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CD8 T cells_IL-2 complex treatment_0 h
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CD8 T cells_IL-2 complex treatment_0 h (untreated)
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strain: C57BL/6J
gender: male
tissue: spleen
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Labeling, hybridization, and scanning were performed at the Center for Array Technologies at the University of Washington.
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| Sample_geo_accession | GSM288809
| | Sample_status | Public on May 17 2008
| | Sample_submission_date | Sep 03 2009
| | Sample_last_update_date | Sep 03 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Jackson Laboratory
| | Sample_treatment_protocol_ch1 | Total CD8 T cells from the spleen of C57BL/6J mice were purified by magnetic cell sorting using the CD8a T cell isolation kit (Miltenyi Biotec), supplemented with biotinylated anti-CD11c, anti-CD19, anti-Gr-1, anti-TCRγδ, anti-I-Ab, and anti-NK1.1 mAbs. The purity was > 95%. Total RNA from the purified CD8 T cells was subject to DNA microarray analysis using GeneChip Mouse genome 430 2.0 array. Target labeling, hybridization and scanning were performed at the Center for Array Technologies at the University of Washington.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from purified CD8 T cells using RNeasy Mini Kit (QIAGEN), then concentrated by EtOH precipitation.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (~4.65ug) was used to create the first strand cDNA using a T7-linked oligo(dT) primer. Once second strand synthesis was complete, an in vitro transcription reaction was performed using biotinylated UTP and CTP in the Affymetrix IVT Kit.
| | Sample_hyb_protocol | Labeled cRNA was processed further as recommended by Affymetrix, where 15 ug of cRNA was fragmented, a hybridization cocktail was assembled with addition of spike-in controls, and chips were hybridized for 16 hours. The chips were then washed and stained with streptavidin-phycoerythrin using the Affymetrix GeneChip System.
| | Sample_scan_protocol | The genechips were placed in the GeneChip Scanner 3000, which scans the chips at a resolution of less than 2 microns.
| | Sample_data_processing | Image processing and expression analysis were performed using Affymetrix GCOS software.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Michael,J,Bevan
| | Sample_contact_laboratory | Bevan
| | Sample_contact_department | Immunology
| | Sample_contact_institute | Univerisity of Washington, HHMI
| | Sample_contact_address | I604H HSC, 1959 NE Pacific Street
| | Sample_contact_city | Seattle
| | Sample_contact_state | WA
| | Sample_contact_zip/postal_code | 98195-7370
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM288nnn/GSM288809/suppl/GSM288809.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM288nnn/GSM288809/suppl/GSM288809.CHP.gz
| | Sample_series_id | GSE11446
| | Sample_data_row_count | 45101
| | |
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GSM288810 | GPL1261 |
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CD8 T cells_IL-2 complex treatment_1 h
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CD8 T cells_IL-2 complex treatment_1 h
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strain: C57BL/6J
gender: male
tissue: spleen
|
Labeling, hybridization, and scanning were performed at the Center for Array Technologies at the University of Washington.
|
| Sample_geo_accession | GSM288810
| | Sample_status | Public on May 17 2008
| | Sample_submission_date | Sep 03 2009
| | Sample_last_update_date | Sep 03 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Jackson Laboratory
| | Sample_treatment_protocol_ch1 | IL-2 complex was prepared by mixing 50 ug anti-IL-2 mAb (S4B6) and 1.5 ug recombinant mouse IL-2, which was then injected i.p. into C57BL/6J mice. Total CD8 T cells from the spleen at 1 h post treatment were purified by magnetic cell sorting using the CD8a T cell isolation kit, supplemented with biotinylated anti-CD11c, anti-CD19, anti-Gr-1, anti-TCRγδ, anti-I-Ab, and anti-NK1.1 mAbs. The purity was > 95%. Total RNA from the purified CD8 T cells was subject to DNA microarray analysis using GeneChip Mouse genome 430 2.0 array. Target labeling, hybridization and scanning were performed at the Center for Array Technologies at the University of Washington.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from purified CD8 T cells using RNeasy Mini Kit (QIAGEN), then concentrated by EtOH precipitation.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (~4.65ug) was used to create the first strand cDNA using a T7-linked oligo(dT) primer. Once second strand synthesis was complete, an in vitro transcription reaction was performed using biotinylated UTP and CTP in the Affymetrix IVT Kit.
| | Sample_hyb_protocol | Labeled cRNA was processed further as recommended by Affymetrix, where 15 ug of cRNA was fragmented, a hybridization cocktail was assembled with addition of spike-in controls, and chips were hybridized for 16 hours. The chips were then washed and stained with streptavidin-phycoerythrin using the Affymetrix GeneChip System.
| | Sample_scan_protocol | The genechips were placed in the GeneChip Scanner 3000, which scans the chips at a resolution of less than 2 microns.
| | Sample_data_processing | Image processing and expression analysis were performed using Affymetrix GCOS software.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Michael,J,Bevan
| | Sample_contact_laboratory | Bevan
| | Sample_contact_department | Immunology
| | Sample_contact_institute | Univerisity of Washington, HHMI
| | Sample_contact_address | I604H HSC, 1959 NE Pacific Street
| | Sample_contact_city | Seattle
| | Sample_contact_state | WA
| | Sample_contact_zip/postal_code | 98195-7370
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM288nnn/GSM288810/suppl/GSM288810.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM288nnn/GSM288810/suppl/GSM288810.CHP.gz
| | Sample_series_id | GSE11446
| | Sample_data_row_count | 45101
| | |
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GSM288811 | GPL1261 |
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CD8 T cells_IL-2 complex treatment_3 h
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CD8 T cells_IL-2 complex treatment_3 h
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strain: C57BL/6J
gender: male
tissue: spleen
|
Labeling, hybridization, and scanning were performed at the Center for Array Technologies at the University of Washington.
|
| Sample_geo_accession | GSM288811
| | Sample_status | Public on May 17 2008
| | Sample_submission_date | Sep 03 2009
| | Sample_last_update_date | Sep 03 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Jackson Laboratory
| | Sample_treatment_protocol_ch1 | IL-2 complex was prepared by mixing 50 ug anti-IL-2 mAb (S4B6) and 1.5 ug recombinant mouse IL-2, which was then injected i.p. into C57BL/6J mice. Total CD8 T cells from the spleen at 3 h post treatment were purified by magnetic cell sorting using the CD8a T cell isolation kit, supplemented with biotinylated anti-CD11c, anti-CD19, anti-Gr-1, anti-TCRγδ, anti-I-Ab, and anti-NK1.1 mAbs. The purity was > 95%. Total RNA from the purified CD8 T cells was subject to DNA microarray analysis using GeneChip Mouse genome 430 2.0 array. Target labeling, hybridization and scanning were performed at the Center for Array Technologies at the University of Washington.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from purified CD8 T cells using RNeasy Mini Kit (QIAGEN), then concentrated by EtOH precipitation.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Total RNA (~4.65ug) was used to create the first strand cDNA using a T7-linked oligo(dT) primer. Once second strand synthesis was complete, an in vitro transcription reaction was performed using biotinylated UTP and CTP in the Affymetrix IVT Kit.
| | Sample_hyb_protocol | Labeled cRNA was processed further as recommended by Affymetrix, where 15 ug of cRNA was fragmented, a hybridization cocktail was assembled with addition of spike-in controls, and chips were hybridized for 16 hours. The chips were then washed and stained with streptavidin-phycoerythrin using the Affymetrix GeneChip System.
| | Sample_scan_protocol | The genechips were placed in the GeneChip Scanner 3000, which scans the chips at a resolution of less than 2 microns.
| | Sample_data_processing | Image processing and expression analysis were performed using Affymetrix GCOS software.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Michael,J,Bevan
| | Sample_contact_laboratory | Bevan
| | Sample_contact_department | Immunology
| | Sample_contact_institute | Univerisity of Washington, HHMI
| | Sample_contact_address | I604H HSC, 1959 NE Pacific Street
| | Sample_contact_city | Seattle
| | Sample_contact_state | WA
| | Sample_contact_zip/postal_code | 98195-7370
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM288nnn/GSM288811/suppl/GSM288811.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM288nnn/GSM288811/suppl/GSM288811.CHP.gz
| | Sample_series_id | GSE11446
| | Sample_data_row_count | 45101
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