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Search results for the GEO ID: GSE11478

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GSM ID

GPL ID

Select for analysis

Title

Source name

Description

Characteristics

GSM289099

GPL1355

NPC_control_1

NPC treated with DMSO for 48 hr

Wister NPC from cerebral cortices of embryo at E15.5

gene expression data from rat NPC treated with control DMSO

Sample_geo_accession	GSM289099
Sample_status	Public on Mar 31 2009
Sample_submission_date	May 16 2008
Sample_last_update_date	Dec 24 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were treated with 1 uM corticosterone or vehicle (DMSO) for 48 hr.
Sample_growth_protocol_ch1	Neuronal progenitor cells were prepared from cerebral cortices of rat embryo at E15.5. Cells were cultured with 20 ng/ml bFGF on fibronectin coated dish.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using TRIzol reagent (Invitrogen). Quality of the RNA was certificated using Agilent 2100 Bioanalyzer (Agilent Technlogies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cDNA were synthesized by GeneChip T7-Oligo(dT) Promoter Primer Kit（Affymetrix) and TaKaRa cDNA Synthesis Kit (TaKaRa Bio) from 3ug total RNA. Biotinylated cRNA were synthesized by IVT Labeling Kit (Affymetrix) .
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 7G.
Sample_data_processing	Single Array Analysis were calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1355
Sample_contact_name	Taira,,Mayanagi
Sample_contact_email	taira@nbiochem.med.osaka-u.ac.jp
Sample_contact_institute	Osaka University Graduate School of Medicine
Sample_contact_address	Yamadaoka 2-2
Sample_contact_city	Suita
Sample_contact_state	Osaka
Sample_contact_zip/postal_code	565-0871
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289099/suppl/GSM289099.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289099/suppl/GSM289099.CHP.gz
Sample_series_id	GSE11478
Sample_data_row_count	31099

GSM289100

GPL1355

NPC_corticosterone_1

NPC treated with 1 uM corticosterone for 48 hr

Wister NPC from cerebral cortices of embryo at E15.5

gene expression data from rat NPC treated with corticosterone

Sample_geo_accession	GSM289100
Sample_status	Public on Mar 31 2009
Sample_submission_date	May 16 2008
Sample_last_update_date	Dec 24 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were treated with 1 uM corticosterone or vehicle (DMSO) for 48 hr.
Sample_growth_protocol_ch1	Neuronal progenitor cells were prepared from cerebral cortices of rat embryo at E15.5. Cells were cultured with 20 ng/ml bFGF on fibronectin coated dish.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using TRIzol reagent (Invitrogen). Quality of the RNA was certificated using Agilent 2100 Bioanalyzer (Agilent Technlogies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cDNA were synthesized by GeneChip T7-Oligo(dT) Promoter Primer Kit（Affymetrix) and TaKaRa cDNA Synthesis Kit (TaKaRa Bio) from 3ug total RNA. Biotinylated cRNA were synthesized by IVT Labeling Kit (Affymetrix) .
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 7G.
Sample_data_processing	Single Array Analysis were calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1355
Sample_contact_name	Taira,,Mayanagi
Sample_contact_email	taira@nbiochem.med.osaka-u.ac.jp
Sample_contact_institute	Osaka University Graduate School of Medicine
Sample_contact_address	Yamadaoka 2-2
Sample_contact_city	Suita
Sample_contact_state	Osaka
Sample_contact_zip/postal_code	565-0871
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289100/suppl/GSM289100.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289100/suppl/GSM289100.CHP.gz
Sample_series_id	GSE11478
Sample_data_row_count	31099

GSM289101

GPL1355

NPC_control_2

NPC treated with DMSO for 48 hr

Wister NPC from cerebral cortices of embryo at E15.5

gene expression data from rat NPC treated with control DMSO

Sample_geo_accession	GSM289101
Sample_status	Public on Mar 31 2009
Sample_submission_date	May 16 2008
Sample_last_update_date	Dec 24 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were treated with 1 uM corticosterone or vehicle (DMSO) for 48 hr.
Sample_growth_protocol_ch1	Neuronal progenitor cells were prepared from cerebral cortices of rat embryo at E15.5. Cells were cultured with 20 ng/ml bFGF on fibronectin coated dish.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using TRIzol reagent (Invitrogen). Quality of the RNA was certificated using Agilent 2100 Bioanalyzer (Agilent Technlogies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cDNA were synthesized by GeneChip T7-Oligo(dT) Promoter Primer Kit（Affymetrix) and TaKaRa cDNA Synthesis Kit (TaKaRa Bio) from 3ug total RNA. Biotinylated cRNA were synthesized by IVT Labeling Kit (Affymetrix) .
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 7G.
Sample_data_processing	Single Array Analysis were calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1355
Sample_contact_name	Taira,,Mayanagi
Sample_contact_email	taira@nbiochem.med.osaka-u.ac.jp
Sample_contact_institute	Osaka University Graduate School of Medicine
Sample_contact_address	Yamadaoka 2-2
Sample_contact_city	Suita
Sample_contact_state	Osaka
Sample_contact_zip/postal_code	565-0871
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289101/suppl/GSM289101.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289101/suppl/GSM289101.CHP.gz
Sample_series_id	GSE11478
Sample_data_row_count	31099

GSM289102

GPL1355

NPC_corticosterone_2

NPC treated with 1 uM corticosterone for 48 hr

Wister NPC from cerebral cortices of embryo at E15.5

gene expression data from rat NPC treated with corticosterone

Sample_geo_accession	GSM289102
Sample_status	Public on Mar 31 2009
Sample_submission_date	May 16 2008
Sample_last_update_date	Dec 24 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were treated with 1 uM corticosterone or vehicle (DMSO) for 48 hr.
Sample_growth_protocol_ch1	Neuronal progenitor cells were prepared from cerebral cortices of rat embryo at E15.5. Cells were cultured with 20 ng/ml bFGF on fibronectin coated dish.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using TRIzol reagent (Invitrogen). Quality of the RNA was certificated using Agilent 2100 Bioanalyzer (Agilent Technlogies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cDNA were synthesized by GeneChip T7-Oligo(dT) Promoter Primer Kit（Affymetrix) and TaKaRa cDNA Synthesis Kit (TaKaRa Bio) from 3ug total RNA. Biotinylated cRNA were synthesized by IVT Labeling Kit (Affymetrix) .
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 7G.
Sample_data_processing	Single Array Analysis were calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1355
Sample_contact_name	Taira,,Mayanagi
Sample_contact_email	taira@nbiochem.med.osaka-u.ac.jp
Sample_contact_institute	Osaka University Graduate School of Medicine
Sample_contact_address	Yamadaoka 2-2
Sample_contact_city	Suita
Sample_contact_state	Osaka
Sample_contact_zip/postal_code	565-0871
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289102/suppl/GSM289102.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289102/suppl/GSM289102.CHP.gz
Sample_series_id	GSE11478
Sample_data_row_count	31099

GSM289103

GPL1355

NPC_control_3

NPC treated with DMSO for 48 hr

Wister NPC from cerebral cortices of embryo at E15.5

gene expression data from rat NPC treated with control DMSO

Sample_geo_accession	GSM289103
Sample_status	Public on Mar 31 2009
Sample_submission_date	May 16 2008
Sample_last_update_date	Dec 24 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were treated with 1 uM corticosterone or vehicle (DMSO) for 48 hr.
Sample_growth_protocol_ch1	Neuronal progenitor cells were prepared from cerebral cortices of rat embryo at E15.5. Cells were cultured with 20 ng/ml bFGF on fibronectin coated dish.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using TRIzol reagent (Invitrogen). Quality of the RNA was certificated using Agilent 2100 Bioanalyzer (Agilent Technlogies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cDNA were synthesized by GeneChip T7-Oligo(dT) Promoter Primer Kit（Affymetrix) and TaKaRa cDNA Synthesis Kit (TaKaRa Bio) from 3ug total RNA. Biotinylated cRNA were synthesized by IVT Labeling Kit (Affymetrix) .
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 7G.
Sample_data_processing	Single Array Analysis were calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1355
Sample_contact_name	Taira,,Mayanagi
Sample_contact_email	taira@nbiochem.med.osaka-u.ac.jp
Sample_contact_institute	Osaka University Graduate School of Medicine
Sample_contact_address	Yamadaoka 2-2
Sample_contact_city	Suita
Sample_contact_state	Osaka
Sample_contact_zip/postal_code	565-0871
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289103/suppl/GSM289103.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289103/suppl/GSM289103.CHP.gz
Sample_series_id	GSE11478
Sample_data_row_count	31099

GSM289104

GPL1355

NPC_corticosterone_3

NPC treated with 1 uM corticosterone for 48 hr

Wister NPC from cerebral cortices of embryo at E15.5

gene expression data from rat NPC treated with corticosterone

Sample_geo_accession	GSM289104
Sample_status	Public on Mar 31 2009
Sample_submission_date	May 16 2008
Sample_last_update_date	Dec 24 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were treated with 1 uM corticosterone or vehicle (DMSO) for 48 hr.
Sample_growth_protocol_ch1	Neuronal progenitor cells were prepared from cerebral cortices of rat embryo at E15.5. Cells were cultured with 20 ng/ml bFGF on fibronectin coated dish.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using TRIzol reagent (Invitrogen). Quality of the RNA was certificated using Agilent 2100 Bioanalyzer (Agilent Technlogies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cDNA were synthesized by GeneChip T7-Oligo(dT) Promoter Primer Kit（Affymetrix) and TaKaRa cDNA Synthesis Kit (TaKaRa Bio) from 3ug total RNA. Biotinylated cRNA were synthesized by IVT Labeling Kit (Affymetrix) .
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 7G.
Sample_data_processing	Single Array Analysis were calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1355
Sample_contact_name	Taira,,Mayanagi
Sample_contact_email	taira@nbiochem.med.osaka-u.ac.jp
Sample_contact_institute	Osaka University Graduate School of Medicine
Sample_contact_address	Yamadaoka 2-2
Sample_contact_city	Suita
Sample_contact_state	Osaka
Sample_contact_zip/postal_code	565-0871
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289104/suppl/GSM289104.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289104/suppl/GSM289104.CHP.gz
Sample_series_id	GSE11478
Sample_data_row_count	31099

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