Result

Search results for the GEO ID: GSE11506

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM289651

GPL570

Bulun 1-3h-

MCF-7 cells treated with vehicle for 3h.

breast adenocarcinoma from 69yr old female caucasian

biological replicate: Bulun 1-3h-,Bulun 2-3h-,Bulun 3-3h-

Sample_geo_accession	GSM289651
Sample_status	Public on May 20 2008
Sample_submission_date	May 20 2008
Sample_last_update_date	May 22 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	After MCF-7 cells were grown to 75% to 80% confluence in MEM supplemented with 10% FBS, the cells were serum starved in DMEM/F-12 without phenol red (Invitrogen) and FBS for 24 h. MCF-7 cells were then treated with vehicle or E2 at a concentration of 10–9 mol/L for 3 and 6 h. All experiments were performed in triplicate.
Sample_growth_protocol_ch1	MCF-7 cells (American Type Culture Collection) were maintained in MEM (Invitrogen) containing 25 units/mL penicillin, 25 units/mL streptomycin, and 10% fetal bovine serum (FBS) at 37°C and 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from the MCF-7 breast cancer cell line using Tri-Reagent (Sigma) according to the manufacturer's instruction. Total RNA samples were treated with DNase I (Ambion) for 20 min at 37°C according to the product manual.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized to Human Genome U133Plus2.0 GeneChips. GeneChips were washed and stained in an Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
Sample_data_processing	Probe level data was converted to probe set expression values using RMA (Irizarry RA et al., Biostatistics 2003 Apr;4(2):249-64).
Sample_platform_id	GPL570
Sample_contact_name	Zhihong,,Lin
Sample_contact_email	z-lin@northwestern.edu
Sample_contact_department	Obstetrics and Gynecology
Sample_contact_institute	Northwestern University
Sample_contact_address	303 East Superior Street
Sample_contact_city	Chicago
Sample_contact_state	IL
Sample_contact_zip/postal_code	60611
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289651/suppl/GSM289651.CEL.gz
Sample_series_id	GSE11506
Sample_data_row_count	54675

GSM289652

GPL570

Bulun 2-3h-

MCF-7 cells treated with vehicle for 3h.

breast adenocarcinoma from 69yr old female caucasian

biological replicate: Bulun 1-3h-,Bulun 2-3h-,Bulun 3-3h-

Sample_geo_accession	GSM289652
Sample_status	Public on May 20 2008
Sample_submission_date	May 20 2008
Sample_last_update_date	May 22 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	After MCF-7 cells were grown to 75% to 80% confluence in MEM supplemented with 10% FBS, the cells were serum starved in DMEM/F-12 without phenol red (Invitrogen) and FBS for 24 h. MCF-7 cells were then treated with vehicle or E2 at a concentration of 10–9 mol/L for 3 and 6 h. All experiments were performed in triplicate.
Sample_growth_protocol_ch1	MCF-7 cells (American Type Culture Collection) were maintained in MEM (Invitrogen) containing 25 units/mL penicillin, 25 units/mL streptomycin, and 10% fetal bovine serum (FBS) at 37°C and 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from the MCF-7 breast cancer cell line using Tri-Reagent (Sigma) according to the manufacturer's instruction. Total RNA samples were treated with DNase I (Ambion) for 20 min at 37°C according to the product manual.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized to Human Genome U133Plus2.0 GeneChips. GeneChips were washed and stained in an Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
Sample_data_processing	Probe level data was converted to probe set expression values using RMA (Irizarry RA et al., Biostatistics 2003 Apr;4(2):249-64).
Sample_platform_id	GPL570
Sample_contact_name	Zhihong,,Lin
Sample_contact_email	z-lin@northwestern.edu
Sample_contact_department	Obstetrics and Gynecology
Sample_contact_institute	Northwestern University
Sample_contact_address	303 East Superior Street
Sample_contact_city	Chicago
Sample_contact_state	IL
Sample_contact_zip/postal_code	60611
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289652/suppl/GSM289652.CEL.gz
Sample_series_id	GSE11506
Sample_data_row_count	54675

GSM289653

GPL570

Bulun 3-3h-

MCF-7 cells treated with vehicle for 3h.

breast adenocarcinoma from 69yr old female caucasian

biological replicate: Bulun 1-3h-,Bulun 2-3h-,Bulun 3-3h-

Sample_geo_accession	GSM289653
Sample_status	Public on May 20 2008
Sample_submission_date	May 20 2008
Sample_last_update_date	May 22 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	After MCF-7 cells were grown to 75% to 80% confluence in MEM supplemented with 10% FBS, the cells were serum starved in DMEM/F-12 without phenol red (Invitrogen) and FBS for 24 h. MCF-7 cells were then treated with vehicle or E2 at a concentration of 10–9 mol/L for 3 and 6 h. All experiments were performed in triplicate.
Sample_growth_protocol_ch1	MCF-7 cells (American Type Culture Collection) were maintained in MEM (Invitrogen) containing 25 units/mL penicillin, 25 units/mL streptomycin, and 10% fetal bovine serum (FBS) at 37°C and 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from the MCF-7 breast cancer cell line using Tri-Reagent (Sigma) according to the manufacturer's instruction. Total RNA samples were treated with DNase I (Ambion) for 20 min at 37°C according to the product manual.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized to Human Genome U133Plus2.0 GeneChips. GeneChips were washed and stained in an Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
Sample_data_processing	Probe level data was converted to probe set expression values using RMA (Irizarry RA et al., Biostatistics 2003 Apr;4(2):249-64).
Sample_platform_id	GPL570
Sample_contact_name	Zhihong,,Lin
Sample_contact_email	z-lin@northwestern.edu
Sample_contact_department	Obstetrics and Gynecology
Sample_contact_institute	Northwestern University
Sample_contact_address	303 East Superior Street
Sample_contact_city	Chicago
Sample_contact_state	IL
Sample_contact_zip/postal_code	60611
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289653/suppl/GSM289653.CEL.gz
Sample_series_id	GSE11506
Sample_data_row_count	54675

GSM289654

GPL570

New 3 hr 1

MCF-7 cells treated with E2 for 3h.

breast adenocarcinoma from 69yr old female caucasian

biological replicate: New 3 hr 1,New 3 hr 2,New 3 hr 3

Sample_geo_accession	GSM289654
Sample_status	Public on May 20 2008
Sample_submission_date	May 20 2008
Sample_last_update_date	May 22 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	After MCF-7 cells were grown to 75% to 80% confluence in MEM supplemented with 10% FBS, the cells were serum starved in DMEM/F-12 without phenol red (Invitrogen) and FBS for 24 h. MCF-7 cells were then treated with vehicle or E2 at a concentration of 10–9 mol/L for 3 and 6 h. All experiments were performed in triplicate.
Sample_growth_protocol_ch1	MCF-7 cells (American Type Culture Collection) were maintained in MEM (Invitrogen) containing 25 units/mL penicillin, 25 units/mL streptomycin, and 10% fetal bovine serum (FBS) at 37°C and 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from the MCF-7 breast cancer cell line using Tri-Reagent (Sigma) according to the manufacturer's instruction. Total RNA samples were treated with DNase I (Ambion) for 20 min at 37°C according to the product manual.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized to Human Genome U133Plus2.0 GeneChips. GeneChips were washed and stained in an Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
Sample_data_processing	Probe level data was converted to probe set expression values using RMA (Irizarry RA et al., Biostatistics 2003 Apr;4(2):249-64).
Sample_platform_id	GPL570
Sample_contact_name	Zhihong,,Lin
Sample_contact_email	z-lin@northwestern.edu
Sample_contact_department	Obstetrics and Gynecology
Sample_contact_institute	Northwestern University
Sample_contact_address	303 East Superior Street
Sample_contact_city	Chicago
Sample_contact_state	IL
Sample_contact_zip/postal_code	60611
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289654/suppl/GSM289654.CEL.gz
Sample_series_id	GSE11506
Sample_data_row_count	54675

GSM289655

GPL570

New 3 hr 2

MCF-7 cells treated with E2 for 3h.

breast adenocarcinoma from 69yr old female caucasian

biological replicate: New 3 hr 1,New 3 hr 2,New 3 hr 3

Sample_geo_accession	GSM289655
Sample_status	Public on May 20 2008
Sample_submission_date	May 20 2008
Sample_last_update_date	May 22 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	After MCF-7 cells were grown to 75% to 80% confluence in MEM supplemented with 10% FBS, the cells were serum starved in DMEM/F-12 without phenol red (Invitrogen) and FBS for 24 h. MCF-7 cells were then treated with vehicle or E2 at a concentration of 10–9 mol/L for 3 and 6 h. All experiments were performed in triplicate.
Sample_growth_protocol_ch1	MCF-7 cells (American Type Culture Collection) were maintained in MEM (Invitrogen) containing 25 units/mL penicillin, 25 units/mL streptomycin, and 10% fetal bovine serum (FBS) at 37°C and 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from the MCF-7 breast cancer cell line using Tri-Reagent (Sigma) according to the manufacturer's instruction. Total RNA samples were treated with DNase I (Ambion) for 20 min at 37°C according to the product manual.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized to Human Genome U133Plus2.0 GeneChips. GeneChips were washed and stained in an Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
Sample_data_processing	Probe level data was converted to probe set expression values using RMA (Irizarry RA et al., Biostatistics 2003 Apr;4(2):249-64).
Sample_platform_id	GPL570
Sample_contact_name	Zhihong,,Lin
Sample_contact_email	z-lin@northwestern.edu
Sample_contact_department	Obstetrics and Gynecology
Sample_contact_institute	Northwestern University
Sample_contact_address	303 East Superior Street
Sample_contact_city	Chicago
Sample_contact_state	IL
Sample_contact_zip/postal_code	60611
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289655/suppl/GSM289655.CEL.gz
Sample_series_id	GSE11506
Sample_data_row_count	54675

GSM289656

GPL570

New 3 hr 3

MCF-7 cells treated with E2 for 3h.

breast adenocarcinoma from 69yr old female caucasian

biological replicate: New 3 hr 1,New 3 hr 2,New 3 hr 3

Sample_geo_accession	GSM289656
Sample_status	Public on May 20 2008
Sample_submission_date	May 20 2008
Sample_last_update_date	May 22 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	After MCF-7 cells were grown to 75% to 80% confluence in MEM supplemented with 10% FBS, the cells were serum starved in DMEM/F-12 without phenol red (Invitrogen) and FBS for 24 h. MCF-7 cells were then treated with vehicle or E2 at a concentration of 10–9 mol/L for 3 and 6 h. All experiments were performed in triplicate.
Sample_growth_protocol_ch1	MCF-7 cells (American Type Culture Collection) were maintained in MEM (Invitrogen) containing 25 units/mL penicillin, 25 units/mL streptomycin, and 10% fetal bovine serum (FBS) at 37°C and 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from the MCF-7 breast cancer cell line using Tri-Reagent (Sigma) according to the manufacturer's instruction. Total RNA samples were treated with DNase I (Ambion) for 20 min at 37°C according to the product manual.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized to Human Genome U133Plus2.0 GeneChips. GeneChips were washed and stained in an Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
Sample_data_processing	Probe level data was converted to probe set expression values using RMA (Irizarry RA et al., Biostatistics 2003 Apr;4(2):249-64).
Sample_platform_id	GPL570
Sample_contact_name	Zhihong,,Lin
Sample_contact_email	z-lin@northwestern.edu
Sample_contact_department	Obstetrics and Gynecology
Sample_contact_institute	Northwestern University
Sample_contact_address	303 East Superior Street
Sample_contact_city	Chicago
Sample_contact_state	IL
Sample_contact_zip/postal_code	60611
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289656/suppl/GSM289656.CEL.gz
Sample_series_id	GSE11506
Sample_data_row_count	54675

GSM289657

GPL570

Bulun 1-6h+

MCF-7 cells treated with E2 for 6h.

breast adenocarcinoma from 69yr old female caucasian

biological replicate: Bulun 1-6h+,Bulun 2-6h+,Bulun 3-6h+

Sample_geo_accession	GSM289657
Sample_status	Public on May 20 2008
Sample_submission_date	May 20 2008
Sample_last_update_date	May 22 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	After MCF-7 cells were grown to 75% to 80% confluence in MEM supplemented with 10% FBS, the cells were serum starved in DMEM/F-12 without phenol red (Invitrogen) and FBS for 24 h. MCF-7 cells were then treated with vehicle or E2 at a concentration of 10–9 mol/L for 3 and 6 h. All experiments were performed in triplicate.
Sample_growth_protocol_ch1	MCF-7 cells (American Type Culture Collection) were maintained in MEM (Invitrogen) containing 25 units/mL penicillin, 25 units/mL streptomycin, and 10% fetal bovine serum (FBS) at 37°C and 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from the MCF-7 breast cancer cell line using Tri-Reagent (Sigma) according to the manufacturer's instruction. Total RNA samples were treated with DNase I (Ambion) for 20 min at 37°C according to the product manual.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized to Human Genome U133Plus2.0 GeneChips. GeneChips were washed and stained in an Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
Sample_data_processing	Probe level data was converted to probe set expression values using RMA (Irizarry RA et al., Biostatistics 2003 Apr;4(2):249-64).
Sample_platform_id	GPL570
Sample_contact_name	Zhihong,,Lin
Sample_contact_email	z-lin@northwestern.edu
Sample_contact_department	Obstetrics and Gynecology
Sample_contact_institute	Northwestern University
Sample_contact_address	303 East Superior Street
Sample_contact_city	Chicago
Sample_contact_state	IL
Sample_contact_zip/postal_code	60611
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289657/suppl/GSM289657.CEL.gz
Sample_series_id	GSE11506
Sample_data_row_count	54675

GSM289658

GPL570

Bulun 2-6h+

MCF-7 cells treated with E2 for 6h.

breast adenocarcinoma from 69yr old female caucasian

biological replicate: Bulun 1-6h+,Bulun 2-6h+,Bulun 3-6h+

Sample_geo_accession	GSM289658
Sample_status	Public on May 20 2008
Sample_submission_date	May 20 2008
Sample_last_update_date	May 22 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	After MCF-7 cells were grown to 75% to 80% confluence in MEM supplemented with 10% FBS, the cells were serum starved in DMEM/F-12 without phenol red (Invitrogen) and FBS for 24 h. MCF-7 cells were then treated with vehicle or E2 at a concentration of 10–9 mol/L for 3 and 6 h. All experiments were performed in triplicate.
Sample_growth_protocol_ch1	MCF-7 cells (American Type Culture Collection) were maintained in MEM (Invitrogen) containing 25 units/mL penicillin, 25 units/mL streptomycin, and 10% fetal bovine serum (FBS) at 37°C and 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from the MCF-7 breast cancer cell line using Tri-Reagent (Sigma) according to the manufacturer's instruction. Total RNA samples were treated with DNase I (Ambion) for 20 min at 37°C according to the product manual.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized to Human Genome U133Plus2.0 GeneChips. GeneChips were washed and stained in an Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
Sample_data_processing	Probe level data was converted to probe set expression values using RMA (Irizarry RA et al., Biostatistics 2003 Apr;4(2):249-64).
Sample_platform_id	GPL570
Sample_contact_name	Zhihong,,Lin
Sample_contact_email	z-lin@northwestern.edu
Sample_contact_department	Obstetrics and Gynecology
Sample_contact_institute	Northwestern University
Sample_contact_address	303 East Superior Street
Sample_contact_city	Chicago
Sample_contact_state	IL
Sample_contact_zip/postal_code	60611
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289658/suppl/GSM289658.CEL.gz
Sample_series_id	GSE11506
Sample_data_row_count	54675

GSM289659

GPL570

Bulun 3-6h+

MCF-7 cells treated with E2 for 6h.

breast adenocarcinoma from 69yr old female caucasian

biological replicate: Bulun 1-6h+,Bulun 2-6h+,Bulun 3-6h+

Sample_geo_accession	GSM289659
Sample_status	Public on May 20 2008
Sample_submission_date	May 20 2008
Sample_last_update_date	May 22 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	After MCF-7 cells were grown to 75% to 80% confluence in MEM supplemented with 10% FBS, the cells were serum starved in DMEM/F-12 without phenol red (Invitrogen) and FBS for 24 h. MCF-7 cells were then treated with vehicle or E2 at a concentration of 10–9 mol/L for 3 and 6 h. All experiments were performed in triplicate.
Sample_growth_protocol_ch1	MCF-7 cells (American Type Culture Collection) were maintained in MEM (Invitrogen) containing 25 units/mL penicillin, 25 units/mL streptomycin, and 10% fetal bovine serum (FBS) at 37°C and 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from the MCF-7 breast cancer cell line using Tri-Reagent (Sigma) according to the manufacturer's instruction. Total RNA samples were treated with DNase I (Ambion) for 20 min at 37°C according to the product manual.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized to Human Genome U133Plus2.0 GeneChips. GeneChips were washed and stained in an Affymetrix Fluidics Station.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
Sample_data_processing	Probe level data was converted to probe set expression values using RMA (Irizarry RA et al., Biostatistics 2003 Apr;4(2):249-64).
Sample_platform_id	GPL570
Sample_contact_name	Zhihong,,Lin
Sample_contact_email	z-lin@northwestern.edu
Sample_contact_department	Obstetrics and Gynecology
Sample_contact_institute	Northwestern University
Sample_contact_address	303 East Superior Street
Sample_contact_city	Chicago
Sample_contact_state	IL
Sample_contact_zip/postal_code	60611
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM289nnn/GSM289659/suppl/GSM289659.CEL.gz
Sample_series_id	GSE11506
Sample_data_row_count	54675

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