Search results for the GEO ID: GSE11559 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM290754 | GPL8300 |
|
NCI-H460_AdFHIT_48hrs_exp1_rep1_sc1
|
Lung cancer cell line NCI-H460, transduced with AdFHIT, 48 hrs
|
Cell line: NCI-H460 (ATCC HTB-177)
Organ: lung
Disease: carcinoma; large cell lung cancer
Derived from metastatic site: pleural effusion
|
RNA was extracted from AdFhit transduced H460 lung cancer cells harvested at 48 hours post infection at a multiplicity of infection of 10. Three independent infections were harvested and RNA preparations were pooled, transcribed into cDNA, labeled, and hybridized following the manufacturer’s protocol to the oligonucleotide microarray Human Gene Chip HG-U95Av2 (Affymetrix)
|
Sample_geo_accession | GSM290754
| Sample_status | Public on Dec 25 2008
| Sample_submission_date | May 23 2008
| Sample_last_update_date | Mar 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Transduced with adenoviral vector carrying human FHIT transgene (48 hrs)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | TRIzol (Invitrogen Life Technologies) extraction, followed by cleanup in RNeasy columns (QIAGEN).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | 10 microg of cDNA were then transcribed with In Vitro Transcription System (Ambion) using biotinylated CTP and UTP.
| Sample_hyb_protocol | 20 microg of fragmented biotin labeled cRNA target were used for Affymetrix GeneChip Array hybridization on HG-U95Av2 chip.
| Sample_scan_protocol | The arrays were washed, stained and scanned according to Affymetrix protocols
| Sample_data_processing | All images were analyzed with MASv5.0
| Sample_platform_id | GPL8300
| Sample_contact_name | Luca,,Roz
| Sample_contact_email | luca.roz@istitutotumori.mi.it
| Sample_contact_laboratory | Cytogenetics and Molecular Cytogenetics
| Sample_contact_department | Experimental Oncology
| Sample_contact_institute | Fondazione IRCCS Isituto Nazionale dei Tumori
| Sample_contact_address | Via Venezian 1
| Sample_contact_city | Milan
| Sample_contact_zip/postal_code | 20133
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM290nnn/GSM290754/suppl/GSM290754.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM290nnn/GSM290754/suppl/GSM290754.CHP.gz
| Sample_series_id | GSE11559
| Sample_data_row_count | 12625
| |
|
GSM291109 | GPL8300 |
|
NCI-H460_AdFHIT_48hrs_exp1_rep2_sc1
|
Lung cancer cell line NCI-H460, transduced with AdFHIT, 48 hrs
|
Cell line: NCI-H460 (ATCC HTB-177)
Organ: lung
Disease: carcinoma; large cell lung cancer
Derived from metastatic site: pleural effusion
|
RNA was extracted from AdFhit transduced H460 lung cancer cells harvested at 48 hours post infection at a multiplicity of infection of 10. Three independent infections were harvested and RNA preparations were pooled, transcribed into cDNA, labeled, and hybridized following the manufacturer's protocol to the oligonucleotide microarray Human Gene Chip HG-U95Av2 (Affymetrix). Two independent hybridizations were performed (rep1 and rep2) and scan images aquired before (sc1) and after (sc2) anti-streptavidin antibody enhancement step.
|
Sample_geo_accession | GSM291109
| Sample_status | Public on Dec 25 2008
| Sample_submission_date | May 26 2008
| Sample_last_update_date | Mar 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Transduced with adenoviral vector carrying human FHIT transgene (48 hrs)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | TRIzol (Invitrogen Life Technologies) extraction, followed by cleanup in RNeasy columns (QIAGEN).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | 10 microg of cDNA were then transcribed with In Vitro Transcription System (Ambion) using biotinylated CTP and UTP.
| Sample_hyb_protocol | 20 microg of fragmented biotin labeled cRNA target were used for Affymetrix GeneChip Array hybridization on HG-U95Av2 chip.
| Sample_scan_protocol | The arrays were washed, stained and scanned according to Affymetrix protocols.
| Sample_data_processing | All images were analyzed with MASv5.0.
| Sample_platform_id | GPL8300
| Sample_contact_name | Luca,,Roz
| Sample_contact_email | luca.roz@istitutotumori.mi.it
| Sample_contact_laboratory | Cytogenetics and Molecular Cytogenetics
| Sample_contact_department | Experimental Oncology
| Sample_contact_institute | Fondazione IRCCS Isituto Nazionale dei Tumori
| Sample_contact_address | Via Venezian 1
| Sample_contact_city | Milan
| Sample_contact_zip/postal_code | 20133
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291109/suppl/GSM291109.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291109/suppl/GSM291109.CHP.gz
| Sample_series_id | GSE11559
| Sample_data_row_count | 12625
| |
|
GSM291110 | GPL8300 |
|
NCI-H460_AdLacZ_48hrs_exp1_rep1_sc1
|
Lung cancer cell line NCI-H460, transduced with AdLACZ, 48 hrs
|
Cell line: NCI-H460 (ATCC HTB-177)
Organ: lung
Disease: carcinoma; large cell lung cancer
Derived from metastatic site: pleural effusion
|
RNA was extracted from AdLacZ transduced H460 lung cancer cells harvested at 48 hours post infection at a multiplicity of infection of 10. Three independent infections were harvested and RNA preparations were pooled, transcribed into cDNA, labeled, and hybridized following the manufacturer's protocol to the oligonucleotide microarray Human Gene Chip HG-U95Av2 (Affymetrix). Two independent hybridizations were performed (rep1 and rep2) and scan images aquired before (sc1) and after (sc2) anti-streptavidin antibody enhancement step.
|
Sample_geo_accession | GSM291110
| Sample_status | Public on Dec 25 2008
| Sample_submission_date | May 26 2008
| Sample_last_update_date | Mar 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Transduced with adenoviral vector carrying LacZ transgene (48 hrs)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | TRIzol (Invitrogen Life Technologies) extraction, followed by cleanup in RNeasy columns (QIAGEN).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | 10 microg of cDNA were then transcribed with In Vitro Transcription System (Ambion) using biotinylated CTP and UTP.
| Sample_hyb_protocol | 20 microg of fragmented biotin labeled cRNA target were used for Affymetrix GeneChip Array hybridization on HG-U95Av2 chip.
| Sample_scan_protocol | The arrays were washed, stained and scanned according to Affymetrix protocols.
| Sample_data_processing | All images were analyzed with MASv5.0.
| Sample_platform_id | GPL8300
| Sample_contact_name | Luca,,Roz
| Sample_contact_email | luca.roz@istitutotumori.mi.it
| Sample_contact_laboratory | Cytogenetics and Molecular Cytogenetics
| Sample_contact_department | Experimental Oncology
| Sample_contact_institute | Fondazione IRCCS Isituto Nazionale dei Tumori
| Sample_contact_address | Via Venezian 1
| Sample_contact_city | Milan
| Sample_contact_zip/postal_code | 20133
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291110/suppl/GSM291110.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291110/suppl/GSM291110.CHP.gz
| Sample_series_id | GSE11559
| Sample_data_row_count | 12625
| |
|
GSM291111 | GPL8300 |
|
NCI-H460_AdLACZ_48hrs_exp1_rep2_sc1
|
Lung cancer cell line NCI-H460, transduced with AdLACZ, 48 hrs
|
Cell line: NCI-H460 (ATCC HTB-177)
Organ: lung
Disease: carcinoma; large cell lung cancer
Derived from metastatic site: pleural effusion
|
RNA was extracted from AdLacZ transduced H460 lung cancer cells harvested at 48 hours post infection at a multiplicity of infection of 10. Three independent infections were harvested and RNA preparations were pooled, transcribed into cDNA, labeled, and hybridized following the manufacturer's protocol to the oligonucleotide microarray Human Gene Chip HG-U95Av2 (Affymetrix). Two independent hybridizations were performed (rep1 and rep2) and scan images aquired before (sc1) and after (sc2) anti-streptavidin antibody enhancement step.
|
Sample_geo_accession | GSM291111
| Sample_status | Public on Dec 25 2008
| Sample_submission_date | May 26 2008
| Sample_last_update_date | Mar 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Transduced with adenoviral vector carrying LacZ transgene (48 hrs)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | TRIzol (Invitrogen Life Technologies) extraction, followed by cleanup in RNeasy columns (QIAGEN).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | 10 microg of cDNA were then transcribed with In Vitro Transcription System (Ambion) using biotinylated CTP and UTP.
| Sample_hyb_protocol | 20 microg of fragmented biotin labeled cRNA target were used for Affymetrix GeneChip Array hybridization on HG-U95Av2 chip.
| Sample_scan_protocol | The arrays were washed, stained and scanned according to Affymetrix protocols.
| Sample_data_processing | All images were analyzed with MASv5.0.
| Sample_platform_id | GPL8300
| Sample_contact_name | Luca,,Roz
| Sample_contact_email | luca.roz@istitutotumori.mi.it
| Sample_contact_laboratory | Cytogenetics and Molecular Cytogenetics
| Sample_contact_department | Experimental Oncology
| Sample_contact_institute | Fondazione IRCCS Isituto Nazionale dei Tumori
| Sample_contact_address | Via Venezian 1
| Sample_contact_city | Milan
| Sample_contact_zip/postal_code | 20133
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291111/suppl/GSM291111.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291111/suppl/GSM291111.CHP.gz
| Sample_series_id | GSE11559
| Sample_data_row_count | 12625
| |
|
GSM291120 | GPL8300 |
|
NCI-H460_AdLACZ_48hrs_exp1_rep1_sc2
|
Lung cancer cell line NCI-H460, transduced with AdLACZ, 48 hrs
|
Cell line: NCI-H460 (ATCC HTB-177)
Organ: lung
Disease: carcinoma; large cell lung cancer
Derived from metastatic site: pleural effusion
|
RNA was extracted from AdLACZ transduced H460 lung cancer cells harvested at 48 hours post infection at a multiplicity of infection of 10. Three independent infections were harvested and RNA preparations were pooled, transcribed into cDNA, labeled, and hybridized following the manufacturer's protocol to the oligonucleotide microarray Human Gene Chip HG-U95Av2 (Affymetrix). Two independent hybridizations were performed (rep1 and rep2) and scan images aquired before (sc1) and after (sc2) anti-streptavidin antibody enhancement step.
|
Sample_geo_accession | GSM291120
| Sample_status | Public on Dec 25 2008
| Sample_submission_date | May 26 2008
| Sample_last_update_date | Mar 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Transduced with adenoviral vector carrying LacZ transgene (48 hrs)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | TRIzol (Invitrogen Life Technologies) extraction, followed by cleanup in RNeasy columns (QIAGEN).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | 10 microg of cDNA were then transcribed with In Vitro Transcription System (Ambion) using biotinylated CTP and UTP.
| Sample_hyb_protocol | 20 microg of fragmented biotin labeled cRNA target were used for Affymetrix GeneChip Array hybridization on HG-U95Av2 chip.
| Sample_scan_protocol | The arrays were washed, stained and scanned according to Affymetrix protocols.
| Sample_data_processing | All images were analyzed with MASv5.0
| Sample_platform_id | GPL8300
| Sample_contact_name | Luca,,Roz
| Sample_contact_email | luca.roz@istitutotumori.mi.it
| Sample_contact_laboratory | Cytogenetics and Molecular Cytogenetics
| Sample_contact_department | Experimental Oncology
| Sample_contact_institute | Fondazione IRCCS Isituto Nazionale dei Tumori
| Sample_contact_address | Via Venezian 1
| Sample_contact_city | Milan
| Sample_contact_zip/postal_code | 20133
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291120/suppl/GSM291120.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291120/suppl/GSM291120.CHP.gz
| Sample_series_id | GSE11559
| Sample_data_row_count | 12625
| |
|
GSM291122 | GPL8300 |
|
NCI-H460_AdLACZ_48hrs_exp1_rep2_sc2
|
Lung cancer cell line NCI-H460, transduced with AdLACZ, 48 hrs
|
Cell line: NCI-H460 (ATCC HTB-177)
Organ: lung
Disease: carcinoma; large cell lung cancer
Derived from metastatic site: pleural effusion
|
RNA was extracted from AdLACZ transduced H460 lung cancer cells harvested at 48 hours post infection at a multiplicity of infection of 10. Three independent infections were harvested and RNA preparations were pooled, transcribed into cDNA, labeled, and hybridized following the manufacturer's protocol to the oligonucleotide microarray Human Gene Chip HG-U95Av2 (Affymetrix). Two independent hybridizations were performed (rep1 and rep2) and scan images aquired before (sc1) and after (sc2) anti-streptavidin antibody enhancement step.
|
Sample_geo_accession | GSM291122
| Sample_status | Public on Dec 25 2008
| Sample_submission_date | May 26 2008
| Sample_last_update_date | Mar 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Transduced with adenoviral vector carrying LacZ transgene (48 hrs)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | TRIzol (Invitrogen Life Technologies) extraction, followed by cleanup in RNeasy columns (QIAGEN).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | 10 microg of cDNA were then transcribed with In Vitro Transcription System (Ambion) using biotinylated CTP and UTP.
| Sample_hyb_protocol | 20 microg of fragmented biotin labeled cRNA target were used for Affymetrix GeneChip Array hybridization on HG-U95Av2 chip.
| Sample_scan_protocol | The arrays were washed, stained and scanned according to Affymetrix protocols.
| Sample_data_processing | All images were analyzed with MASv5.0
| Sample_platform_id | GPL8300
| Sample_contact_name | Luca,,Roz
| Sample_contact_email | luca.roz@istitutotumori.mi.it
| Sample_contact_laboratory | Cytogenetics and Molecular Cytogenetics
| Sample_contact_department | Experimental Oncology
| Sample_contact_institute | Fondazione IRCCS Isituto Nazionale dei Tumori
| Sample_contact_address | Via Venezian 1
| Sample_contact_city | Milan
| Sample_contact_zip/postal_code | 20133
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291122/suppl/GSM291122.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291122/suppl/GSM291122.CHP.gz
| Sample_series_id | GSE11559
| Sample_data_row_count | 12625
| |
|
GSM291126 | GPL8300 |
|
NCI-H460_AdFHIT_48hrs_exp1_rep2_sc2
|
Lung cancer cell line NCI-H460, transduced with AdFHIT, 48 hrs
|
Cell line: NCI-H460 (ATCC HTB-177)
Organ: lung
Disease: carcinoma; large cell lung cancer
Derived from metastatic site: pleural effusion
|
RNA was extracted from AdFHIT transduced H460 lung cancer cells harvested at 48 hours post infection at a multiplicity of infection of 10. Three independent infections were harvested and RNA preparations were pooled, transcribed into cDNA, labeled, and hybridized following the manufacturer's protocol to the oligonucleotide microarray Human Gene Chip HG-U95Av2 (Affymetrix). Two independent hybridizations were performed (rep1 and rep2) and scan images aquired before (sc1) and after (sc2) anti-streptavidin antibody enhancement step.
|
Sample_geo_accession | GSM291126
| Sample_status | Public on Dec 25 2008
| Sample_submission_date | May 26 2008
| Sample_last_update_date | Mar 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Transduced with adenoviral vector carrying human FHIT transgene (48 hrs)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | TRIzol (Invitrogen Life Technologies) extraction, followed by cleanup in RNeasy columns (QIAGEN).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | 10 microg of cDNA were then transcribed with In Vitro Transcription System (Ambion) using biotinylated CTP and UTP.
| Sample_hyb_protocol | 20 microg of fragmented biotin labeled cRNA target were used for Affymetrix GeneChip Array hybridization on HG-U95Av2 chip.
| Sample_scan_protocol | The arrays were washed, stained and scanned according to Affymetrix protocols.
| Sample_data_processing | All images were analyzed with MASv5.0
| Sample_platform_id | GPL8300
| Sample_contact_name | Luca,,Roz
| Sample_contact_email | luca.roz@istitutotumori.mi.it
| Sample_contact_laboratory | Cytogenetics and Molecular Cytogenetics
| Sample_contact_department | Experimental Oncology
| Sample_contact_institute | Fondazione IRCCS Isituto Nazionale dei Tumori
| Sample_contact_address | Via Venezian 1
| Sample_contact_city | Milan
| Sample_contact_zip/postal_code | 20133
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291126/suppl/GSM291126.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291126/suppl/GSM291126.CHP.gz
| Sample_series_id | GSE11559
| Sample_data_row_count | 12625
| |
|
GSM291129 | GPL8300 |
|
NCI-H460_AdFHIT_48hrs_exp1_rep1_sc2
|
Lung cancer cell line NCI-H460, transduced with AdFHIT, 48 hrs
|
Cell line: NCI-H460 (ATCC HTB-177)
Organ: lung
Disease: carcinoma; large cell lung cancer
Derived from metastatic site: pleural effusion
|
RNA was extracted from AdFHIT transduced H460 lung cancer cells harvested at 48 hours post infection at a multiplicity of infection of 10. Three independent infections were harvested and RNA preparations were pooled, transcribed into cDNA, labeled, and hybridized following the manufacturer's protocol to the oligonucleotide microarray Human Gene Chip HG-U95Av2 (Affymetrix). Two independent hybridizations were performed (rep1 and rep2) and scan images aquired before (sc1) and after (sc2) anti-streptavidin antibody enhancement step.
|
Sample_geo_accession | GSM291129
| Sample_status | Public on Dec 25 2008
| Sample_submission_date | May 26 2008
| Sample_last_update_date | Mar 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Transduced with adenoviral vector carrying human FHIT transgene (48 hrs)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | TRIzol (Invitrogen Life Technologies) extraction, followed by cleanup in RNeasy columns (QIAGEN).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | 10 microg of cDNA were then transcribed with In Vitro Transcription System (Ambion) using biotinylated CTP and UTP.
| Sample_hyb_protocol | 20 microg of fragmented biotin labeled cRNA target were used for Affymetrix GeneChip Array hybridization on HG-U95Av2 chip.
| Sample_scan_protocol | The arrays were washed, stained and scanned according to Affymetrix protocols.
| Sample_data_processing | All images were analyzed with MASv5.0
| Sample_platform_id | GPL8300
| Sample_contact_name | Luca,,Roz
| Sample_contact_email | luca.roz@istitutotumori.mi.it
| Sample_contact_laboratory | Cytogenetics and Molecular Cytogenetics
| Sample_contact_department | Experimental Oncology
| Sample_contact_institute | Fondazione IRCCS Isituto Nazionale dei Tumori
| Sample_contact_address | Via Venezian 1
| Sample_contact_city | Milan
| Sample_contact_zip/postal_code | 20133
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291129/suppl/GSM291129.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291129/suppl/GSM291129.CHP.gz
| Sample_series_id | GSE11559
| Sample_data_row_count | 12625
| |
|
GSM291130 | GPL8300 |
|
NCI-H460_AdFHIT_48hrs_exp2_rep1_sc1
|
Lung cancer cell line NCI-H460, transduced with AdFHIT, 48 hrs
|
Cell line: NCI-H460 (ATCC HTB-177)
Organ: lung
Disease: carcinoma; large cell lung cancer
Derived from metastatic site: pleural effusion
|
RNA was extracted from AdFHIT transduced H460 lung cancer cells harvested at 48 hours post infection at a multiplicity of infection of 10. Three independent infections were harvested and RNA preparations were pooled, transcribed into cDNA, labeled, and hybridized following the manufacturer's protocol to the oligonucleotide microarray Human Gene Chip HG-U95Av2 (Affymetrix). Two independent hybridizations were performed (rep1 and rep2) and scan images aquired before (sc1) and after (sc2) anti-streptavidin antibody enhancement step.
The whole procedure was repeated twice (starting from thawing of new aliquot of NCI-H460 cells) resulting in two independent experiments (exp1 and exp2).
|
Sample_geo_accession | GSM291130
| Sample_status | Public on Dec 25 2008
| Sample_submission_date | May 26 2008
| Sample_last_update_date | Mar 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Transduced with adenoviral vector carrying human FHIT transgene (48 hrs)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | TRIzol (Invitrogen Life Technologies) extraction, followed by cleanup in RNeasy columns (QIAGEN).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | 10 microg of cDNA were then transcribed with In Vitro Transcription System (Ambion) using biotinylated CTP and UTP.
| Sample_hyb_protocol | 20 microg of fragmented biotin labeled cRNA target were used for Affymetrix GeneChip Array hybridization on HG-U95Av2 chip.
| Sample_scan_protocol | The arrays were washed, stained and scanned according to Affymetrix protocols.
| Sample_data_processing | All images were analyzed with MASv5.0
| Sample_platform_id | GPL8300
| Sample_contact_name | Luca,,Roz
| Sample_contact_email | luca.roz@istitutotumori.mi.it
| Sample_contact_laboratory | Cytogenetics and Molecular Cytogenetics
| Sample_contact_department | Experimental Oncology
| Sample_contact_institute | Fondazione IRCCS Isituto Nazionale dei Tumori
| Sample_contact_address | Via Venezian 1
| Sample_contact_city | Milan
| Sample_contact_zip/postal_code | 20133
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291130/suppl/GSM291130.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291130/suppl/GSM291130.CHP.gz
| Sample_series_id | GSE11559
| Sample_data_row_count | 12625
| |
|
GSM291131 | GPL8300 |
|
NCI-H460_AdFHIT_48hrs_exp2_rep2_sc1
|
Lung cancer cell line NCI-H460, transduced with AdFHIT, 48 hrs
|
Cell line: NCI-H460 (ATCC HTB-177)
Organ: lung
Disease: carcinoma; large cell lung cancer
Derived from metastatic site: pleural effusion
|
RNA was extracted from AdFHIT transduced H460 lung cancer cells harvested at 48 hours post infection at a multiplicity of infection of 10. Three independent infections were harvested and RNA preparations were pooled, transcribed into cDNA, labeled, and hybridized following the manufacturer's protocol to the oligonucleotide microarray Human Gene Chip HG-U95Av2 (Affymetrix). Two independent hybridizations were performed (rep1 and rep2) and scan images aquired before (sc1) and after (sc2) anti-streptavidin antibody enhancement step.
The whole procedure was repeated twice (starting from thawing of new aliquot of NCI-H460 cells) resulting in two independent experiments (exp1 and exp2).
|
Sample_geo_accession | GSM291131
| Sample_status | Public on Dec 25 2008
| Sample_submission_date | May 26 2008
| Sample_last_update_date | Mar 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Transduced with adenoviral vector carrying human FHIT transgene (48 hrs)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | TRIzol (Invitrogen Life Technologies) extraction, followed by cleanup in RNeasy columns (QIAGEN).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | 10 microg of cDNA were then transcribed with In Vitro Transcription System (Ambion) using biotinylated CTP and UTP.
| Sample_hyb_protocol | 20 microg of fragmented biotin labeled cRNA target were used for Affymetrix GeneChip Array hybridization on HG-U95Av2 chip.
| Sample_scan_protocol | The arrays were washed, stained and scanned according to Affymetrix protocols.
| Sample_data_processing | All images were analyzed with MASv5.0
| Sample_platform_id | GPL8300
| Sample_contact_name | Luca,,Roz
| Sample_contact_email | luca.roz@istitutotumori.mi.it
| Sample_contact_laboratory | Cytogenetics and Molecular Cytogenetics
| Sample_contact_department | Experimental Oncology
| Sample_contact_institute | Fondazione IRCCS Isituto Nazionale dei Tumori
| Sample_contact_address | Via Venezian 1
| Sample_contact_city | Milan
| Sample_contact_zip/postal_code | 20133
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291131/suppl/GSM291131.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291131/suppl/GSM291131.CHP.gz
| Sample_series_id | GSE11559
| Sample_data_row_count | 12625
| |
|
GSM291132 | GPL8300 |
|
NCI-H460_AdFHIT_48hrs_exp2_rep1_sc2
|
Lung cancer cell line NCI-H460, transduced with AdFHIT, 48 hrs
|
Cell line: NCI-H460 (ATCC HTB-177)
Organ: lung
Disease: carcinoma; large cell lung cancer
Derived from metastatic site: pleural effusion
|
RNA was extracted from AdFHIT transduced H460 lung cancer cells harvested at 48 hours post infection at a multiplicity of infection of 10. Three independent infections were harvested and RNA preparations were pooled, transcribed into cDNA, labeled, and hybridized following the manufacturer's protocol to the oligonucleotide microarray Human Gene Chip HG-U95Av2 (Affymetrix). Two independent hybridizations were performed (rep1 and rep2) and scan images aquired before (sc1) and after (sc2) anti-streptavidin antibody enhancement step.
The whole procedure was repeated twice (starting from thawing of new aliquot of NCI-H460 cells) resulting in two independent experiments (exp1 and exp2).
|
Sample_geo_accession | GSM291132
| Sample_status | Public on Dec 25 2008
| Sample_submission_date | May 26 2008
| Sample_last_update_date | Mar 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Transduced with adenoviral vector carrying human FHIT transgene (48 hrs)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | TRIzol (Invitrogen Life Technologies) extraction, followed by cleanup in RNeasy columns (QIAGEN).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | 10 microg of cDNA were then transcribed with In Vitro Transcription System (Ambion) using biotinylated CTP and UTP.
| Sample_hyb_protocol | 20 microg of fragmented biotin labeled cRNA target were used for Affymetrix GeneChip Array hybridization on HG-U95Av2 chip.
| Sample_scan_protocol | The arrays were washed, stained and scanned according to Affymetrix protocols.
| Sample_data_processing | All images were analyzed with MASv5.0
| Sample_platform_id | GPL8300
| Sample_contact_name | Luca,,Roz
| Sample_contact_email | luca.roz@istitutotumori.mi.it
| Sample_contact_laboratory | Cytogenetics and Molecular Cytogenetics
| Sample_contact_department | Experimental Oncology
| Sample_contact_institute | Fondazione IRCCS Isituto Nazionale dei Tumori
| Sample_contact_address | Via Venezian 1
| Sample_contact_city | Milan
| Sample_contact_zip/postal_code | 20133
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291132/suppl/GSM291132.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291132/suppl/GSM291132.CHP.gz
| Sample_series_id | GSE11559
| Sample_data_row_count | 12625
| |
|
GSM291133 | GPL8300 |
|
NCI-H460_AdFHIT_48hrs_exp2_rep2_sc2
|
Lung cancer cell line NCI-H460, transduced with AdFHIT, 48 hrs
|
Cell line: NCI-H460 (ATCC HTB-177)
Organ: lung
Disease: carcinoma; large cell lung cancer
Derived from metastatic site: pleural effusion
|
RNA was extracted from AdFhit transduced H460 lung cancer cells harvested at 48 hours post infection at a multiplicity of infection of 10. Three independent infections were harvested and RNA preparations were pooled, transcribed into cDNA, labeled, and hybridized following the manufacturer's protocol to the oligonucleotide microarray Human Gene Chip HG-U95Av2 (Affymetrix). Two independent hybridizations were performed (rep1 and rep2) and scan images aquired before (sc1) and after (sc2) anti-streptavidin antibody enhancement step.
The whole procedure was repeated twice (starting from thawing of new aliquot of NCI-H460 cells) resulting in two independent experiments (exp1 and exp2).
|
Sample_geo_accession | GSM291133
| Sample_status | Public on Dec 25 2008
| Sample_submission_date | May 26 2008
| Sample_last_update_date | Mar 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Transduced with adenoviral vector carrying human FHIT transgene (48 hrs)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | TRIzol (Invitrogen Life Technologies) extraction, followed by cleanup in RNeasy columns (QIAGEN).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | 10 microg of cDNA were then transcribed with In Vitro Transcription System (Ambion) using biotinylated CTP and UTP.
| Sample_hyb_protocol | 20 microg of fragmented biotin labeled cRNA target were used for Affymetrix GeneChip Array hybridization on HG-U95Av2 chip.
| Sample_scan_protocol | The arrays were washed, stained and scanned according to Affymetrix protocols.
| Sample_data_processing | All images were analyzed with MASv5.0
| Sample_platform_id | GPL8300
| Sample_contact_name | Luca,,Roz
| Sample_contact_email | luca.roz@istitutotumori.mi.it
| Sample_contact_laboratory | Cytogenetics and Molecular Cytogenetics
| Sample_contact_department | Experimental Oncology
| Sample_contact_institute | Fondazione IRCCS Isituto Nazionale dei Tumori
| Sample_contact_address | Via Venezian 1
| Sample_contact_city | Milan
| Sample_contact_zip/postal_code | 20133
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291133/suppl/GSM291133.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291133/suppl/GSM291133.CHP.gz
| Sample_series_id | GSE11559
| Sample_data_row_count | 12625
| |
|
GSM291134 | GPL8300 |
|
NCI-H460_AdLACZ_48hrs_exp2_rep1_sc1
|
Lung cancer cell line NCI-H460, transduced with AdLACZ, 48 hrs
|
Cell line: NCI-H460 (ATCC HTB-177)
Organ: lung
Disease: carcinoma; large cell lung cancer
Derived from metastatic site: pleural effusion
|
RNA was extracted from AdLACZ transduced H460 lung cancer cells harvested at 48 hours post infection at a multiplicity of infection of 10. Three independent infections were harvested and RNA preparations were pooled, transcribed into cDNA, labeled, and hybridized following the manufacturer's protocol to the oligonucleotide microarray Human Gene Chip HG-U95Av2 (Affymetrix). Two independent hybridizations were performed (rep1 and rep2) and scan images aquired before (sc1) and after (sc2) anti-streptavidin antibody enhancement step.
The whole procedure was repeated twice (starting from thawing of new aliquot of NCI-H460 cells) resulting in two independent experiments (exp1 and exp2).
|
Sample_geo_accession | GSM291134
| Sample_status | Public on Dec 25 2008
| Sample_submission_date | May 26 2008
| Sample_last_update_date | Mar 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Transduced with adenoviral vector carrying LACZ transgene (48 hrs)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | TRIzol (Invitrogen Life Technologies) extraction, followed by cleanup in RNeasy columns (QIAGEN).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | 10 microg of cDNA were then transcribed with In Vitro Transcription System (Ambion) using biotinylated CTP and UTP.
| Sample_hyb_protocol | 20 microg of fragmented biotin labeled cRNA target were used for Affymetrix GeneChip Array hybridization on HG-U95Av2 chip.
| Sample_scan_protocol | The arrays were washed, stained and scanned according to Affymetrix protocols.
| Sample_data_processing | All images were analyzed with MASv5.0
| Sample_platform_id | GPL8300
| Sample_contact_name | Luca,,Roz
| Sample_contact_email | luca.roz@istitutotumori.mi.it
| Sample_contact_laboratory | Cytogenetics and Molecular Cytogenetics
| Sample_contact_department | Experimental Oncology
| Sample_contact_institute | Fondazione IRCCS Isituto Nazionale dei Tumori
| Sample_contact_address | Via Venezian 1
| Sample_contact_city | Milan
| Sample_contact_zip/postal_code | 20133
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291134/suppl/GSM291134.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291134/suppl/GSM291134.CHP.gz
| Sample_series_id | GSE11559
| Sample_data_row_count | 12625
| |
|
GSM291136 | GPL8300 |
|
NCI-H460_AdLACZ_48hrs_exp2_rep2_sc1
|
Lung cancer cell line NCI-H460, transduced with AdLACZ, 48 hrs
|
Cell line: NCI-H460 (ATCC HTB-177)
Organ: lung
Disease: carcinoma; large cell lung cancer
Derived from metastatic site: pleural effusion
|
RNA was extracted from AdLACZ transduced H460 lung cancer cells harvested at 48 hours post infection at a multiplicity of infection of 10. Three independent infections were harvested and RNA preparations were pooled, transcribed into cDNA, labeled, and hybridized following the manufacturer's protocol to the oligonucleotide microarray Human Gene Chip HG-U95Av2 (Affymetrix). Two independent hybridizations were performed (rep1 and rep2) and scan images aquired before (sc1) and after (sc2) anti-streptavidin antibody enhancement step.
The whole procedure was repeated twice (starting from thawing of new aliquot of NCI-H460 cells) resulting in two independent experiments (exp1 and exp2).
|
Sample_geo_accession | GSM291136
| Sample_status | Public on Dec 25 2008
| Sample_submission_date | May 26 2008
| Sample_last_update_date | Mar 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Transduced with adenoviral vector carrying LacZ transgene (48 hrs)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | TRIzol (Invitrogen Life Technologies) extraction, followed by cleanup in RNeasy columns (QIAGEN).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | 10 microg of cDNA were then transcribed with In Vitro Transcription System (Ambion) using biotinylated CTP and UTP.
| Sample_hyb_protocol | 20 microg of fragmented biotin labeled cRNA target were used for Affymetrix GeneChip Array hybridization on HG-U95Av2 chip.
| Sample_scan_protocol | The arrays were washed, stained and scanned according to Affymetrix protocols.
| Sample_data_processing | All images were analyzed with MASv5.0
| Sample_platform_id | GPL8300
| Sample_contact_name | Luca,,Roz
| Sample_contact_email | luca.roz@istitutotumori.mi.it
| Sample_contact_laboratory | Cytogenetics and Molecular Cytogenetics
| Sample_contact_department | Experimental Oncology
| Sample_contact_institute | Fondazione IRCCS Isituto Nazionale dei Tumori
| Sample_contact_address | Via Venezian 1
| Sample_contact_city | Milan
| Sample_contact_zip/postal_code | 20133
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291136/suppl/GSM291136.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291136/suppl/GSM291136.CHP.gz
| Sample_series_id | GSE11559
| Sample_data_row_count | 12625
| |
|
GSM291138 | GPL8300 |
|
NCI-H460_AdLACZ_48hrs_exp2_rep1_sc2
|
Lung cancer cell line NCI-H460, transduced with AdLACZ, 48 hrs
|
Cell line: NCI-H460 (ATCC HTB-177)
Organ: lung
Disease: carcinoma; large cell lung cancer
Derived from metastatic site: pleural effusion
|
RNA was extracted from AdLACZ transduced H460 lung cancer cells harvested at 48 hours post infection at a multiplicity of infection of 10. Three independent infections were harvested and RNA preparations were pooled, transcribed into cDNA, labeled, and hybridized following the manufacturer's protocol to the oligonucleotide microarray Human Gene Chip HG-U95Av2 (Affymetrix). Two independent hybridizations were performed (rep1 and rep2) and scan images aquired before (sc1) and after (sc2) anti-streptavidin antibody enhancement step.
The whole procedure was repeated twice (starting from thawing of new aliquot of NCI-H460 cells) resulting in two independent experiments (exp1 and exp2).
|
Sample_geo_accession | GSM291138
| Sample_status | Public on Dec 25 2008
| Sample_submission_date | May 26 2008
| Sample_last_update_date | Mar 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Transduced with adenoviral vector carrying LacZ transgene (48 hrs)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | TRIzol (Invitrogen Life Technologies) extraction, followed by cleanup in RNeasy columns (QIAGEN).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | 10 microg of cDNA were then transcribed with In Vitro Transcription System (Ambion) using biotinylated CTP and UTP.
| Sample_hyb_protocol | 20 microg of fragmented biotin labeled cRNA target were used for Affymetrix GeneChip Array hybridization on HG-U95Av2 chip.
| Sample_scan_protocol | The arrays were washed, stained and scanned according to Affymetrix protocols.
| Sample_data_processing | All images were analyzed with MASv5.0
| Sample_platform_id | GPL8300
| Sample_contact_name | Luca,,Roz
| Sample_contact_email | luca.roz@istitutotumori.mi.it
| Sample_contact_laboratory | Cytogenetics and Molecular Cytogenetics
| Sample_contact_department | Experimental Oncology
| Sample_contact_institute | Fondazione IRCCS Isituto Nazionale dei Tumori
| Sample_contact_address | Via Venezian 1
| Sample_contact_city | Milan
| Sample_contact_zip/postal_code | 20133
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291138/suppl/GSM291138.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291138/suppl/GSM291138.CHP.gz
| Sample_series_id | GSE11559
| Sample_data_row_count | 12625
| |
|
GSM291139 | GPL8300 |
|
NCI-H460_AdLACZ_48hrs_exp2_rep2_sc2
|
Lung cancer cell line NCI-H460, transduced with AdLACZ, 48 hrs
|
Cell line: NCI-H460 (ATCC HTB-177)
Organ: lung
Disease: carcinoma; large cell lung cancer
Derived from metastatic site: pleural effusion
|
RNA was extracted from AdLACZ transduced H460 lung cancer cells harvested at 48 hours post infection at a multiplicity of infection of 10. Three independent infections were harvested and RNA preparations were pooled, transcribed into cDNA, labeled, and hybridized following the manufacturer's protocol to the oligonucleotide microarray Human Gene Chip HG-U95Av2 (Affymetrix). Two independent hybridizations were performed (rep1 and rep2) and scan images aquired before (sc1) and after (sc2) anti-streptavidin antibody enhancement step.
The whole procedure was repeated twice (starting from thawing of new aliquot of NCI-H460 cells) resulting in two independent experiments (exp1 and exp2).
|
Sample_geo_accession | GSM291139
| Sample_status | Public on Dec 25 2008
| Sample_submission_date | May 26 2008
| Sample_last_update_date | Mar 16 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Transduced with adenoviral vector carrying LacZ transgene (48 hrs)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | TRIzol (Invitrogen Life Technologies) extraction, followed by cleanup in RNeasy columns (QIAGEN).
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | 10 microg of cDNA were then transcribed with In Vitro Transcription System (Ambion) using biotinylated CTP and UTP.
| Sample_hyb_protocol | 20 microg of fragmented biotin labeled cRNA target were used for Affymetrix GeneChip Array hybridization on HG-U95Av2 chip.
| Sample_scan_protocol | The arrays were washed, stained and scanned according to Affymetrix protocols.
| Sample_data_processing | All images were analyzed with MASv5.0
| Sample_platform_id | GPL8300
| Sample_contact_name | Luca,,Roz
| Sample_contact_email | luca.roz@istitutotumori.mi.it
| Sample_contact_laboratory | Cytogenetics and Molecular Cytogenetics
| Sample_contact_department | Experimental Oncology
| Sample_contact_institute | Fondazione IRCCS Isituto Nazionale dei Tumori
| Sample_contact_address | Via Venezian 1
| Sample_contact_city | Milan
| Sample_contact_zip/postal_code | 20133
| Sample_contact_country | Italy
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291139/suppl/GSM291139.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM291nnn/GSM291139/suppl/GSM291139.CHP.gz
| Sample_series_id | GSE11559
| Sample_data_row_count | 12625
| |
|
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