Result

Search results for the GEO ID: GSE11589

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM292046

GPL1261

Irradiated EL08-1D2, biological rep 1

Embryonic E11.5 liver cell line EL08-1D2

Embryonic liver stromal cell clone

Gene expression data from embryo-deived stromal cells

Sample_geo_accession	GSM292046
Sample_status	Public on Jul 07 2008
Sample_submission_date	May 29 2008
Sample_last_update_date	Jul 07 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Confluent cultures were irradiated (30 Gy), medium exchanged completely and grown for another week. One day prior to harvest, medium was again completely refreshed.
Sample_growth_protocol_ch1	Embryo-derived stromal cell lines were grown to confluence as described (Oostendorp et al., Stem Cells. 2005;23:842:50)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on MOE430 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Robert,A.J.,Oostendorp
Sample_contact_email	oostendorp@lrz.tum.de
Sample_contact_phone	498941406056
Sample_contact_fax	498941406057
Sample_contact_laboratory	3rd Department of Internal Medicine
Sample_contact_department	Klinikum rechts der Isar
Sample_contact_institute	Technische Universitaet Muenchen
Sample_contact_address	Ismaningerstrasse 22
Sample_contact_city	Muenchen
Sample_contact_zip/postal_code	81675
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM292nnn/GSM292046/suppl/GSM292046.CEL.gz
Sample_series_id	GSE11589
Sample_data_row_count	45101

GSM292047

GPL1261

Irradiated EL08-1D2, biological rep 2

Embryonic E11.5 liver cell line EL08-1D2

Embryonic liver stromal cell clone

Gene expression data from embryo-deived stromal cells

Sample_geo_accession	GSM292047
Sample_status	Public on Jul 07 2008
Sample_submission_date	May 29 2008
Sample_last_update_date	Jul 07 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Confluent cultures were irradiated (30 Gy), medium exchanged completely and grown for another week. One day prior to harvest, medium was again completely refreshed.
Sample_growth_protocol_ch1	Embryo-derived stromal cell lines were grown to confluence as described (Oostendorp et al., Stem Cells. 2005;23:842:50)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on MOE430 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Robert,A.J.,Oostendorp
Sample_contact_email	oostendorp@lrz.tum.de
Sample_contact_phone	498941406056
Sample_contact_fax	498941406057
Sample_contact_laboratory	3rd Department of Internal Medicine
Sample_contact_department	Klinikum rechts der Isar
Sample_contact_institute	Technische Universitaet Muenchen
Sample_contact_address	Ismaningerstrasse 22
Sample_contact_city	Muenchen
Sample_contact_zip/postal_code	81675
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM292nnn/GSM292047/suppl/GSM292047.CEL.gz
Sample_series_id	GSE11589
Sample_data_row_count	45101

GSM292048

GPL1261

Irradiated UG26-1B6, biological rep 1

Embryonic E11.5 urogenital ridge cell line UG26-1B6

Embryonic urogenital ridge stromal cell clone

Gene expression data from embryo-deived stromal cells

Sample_geo_accession	GSM292048
Sample_status	Public on Jul 07 2008
Sample_submission_date	May 29 2008
Sample_last_update_date	Jul 07 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Confluent cultures were irradiated (30 Gy), medium exchanged completely and grown for another week. One day prior to harvest, medium was again completely refreshed.
Sample_growth_protocol_ch1	Embryo-derived stromal cell lines were grown to confluence as described (Oostendorp et al., Stem Cells. 2005;23:842:50)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on MOE430 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Robert,A.J.,Oostendorp
Sample_contact_email	oostendorp@lrz.tum.de
Sample_contact_phone	498941406056
Sample_contact_fax	498941406057
Sample_contact_laboratory	3rd Department of Internal Medicine
Sample_contact_department	Klinikum rechts der Isar
Sample_contact_institute	Technische Universitaet Muenchen
Sample_contact_address	Ismaningerstrasse 22
Sample_contact_city	Muenchen
Sample_contact_zip/postal_code	81675
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM292nnn/GSM292048/suppl/GSM292048.CEL.gz
Sample_series_id	GSE11589
Sample_data_row_count	45101

GSM292049

GPL1261

Irradiated UG26-1B6, biological rep 2

Embryonic E11.5 urogenital ridge cell line UG26-1B6

Embryonic urogenital ridge stromal cell clone

Gene expression data from embryo-deived stromal cells

Sample_geo_accession	GSM292049
Sample_status	Public on Jul 07 2008
Sample_submission_date	May 29 2008
Sample_last_update_date	Jul 07 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Confluent cultures were irradiated (30 Gy), medium exchanged completely and grown for another week. One day prior to harvest, medium was again completely refreshed.
Sample_growth_protocol_ch1	Embryo-derived stromal cell lines were grown to confluence as described (Oostendorp et al., Stem Cells. 2005;23:842:50)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on MOE430 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Robert,A.J.,Oostendorp
Sample_contact_email	oostendorp@lrz.tum.de
Sample_contact_phone	498941406056
Sample_contact_fax	498941406057
Sample_contact_laboratory	3rd Department of Internal Medicine
Sample_contact_department	Klinikum rechts der Isar
Sample_contact_institute	Technische Universitaet Muenchen
Sample_contact_address	Ismaningerstrasse 22
Sample_contact_city	Muenchen
Sample_contact_zip/postal_code	81675
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM292nnn/GSM292049/suppl/GSM292049.CEL.gz
Sample_series_id	GSE11589
Sample_data_row_count	45101

GSM292050

GPL1261

Irradiated AM20-1B4

Embryonic E10.5 aorta/mesenchyme cell line AM20-1B4

Embryonic aorta/mesenchyme stromal cell clone

Gene expression data from embryo-deived stromal cells

Sample_geo_accession	GSM292050
Sample_status	Public on Jul 07 2008
Sample_submission_date	May 29 2008
Sample_last_update_date	Jul 07 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Confluent cultures were irradiated (30 Gy), medium exchanged completely and grown for another week. One day prior to harvest, medium was again completely refreshed.
Sample_growth_protocol_ch1	Embryo-derived stromal cell lines were grown to confluence as described (Oostendorp et al., Stem Cells. 2005;23:842:50)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on MOE430 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Robert,A.J.,Oostendorp
Sample_contact_email	oostendorp@lrz.tum.de
Sample_contact_phone	498941406056
Sample_contact_fax	498941406057
Sample_contact_laboratory	3rd Department of Internal Medicine
Sample_contact_department	Klinikum rechts der Isar
Sample_contact_institute	Technische Universitaet Muenchen
Sample_contact_address	Ismaningerstrasse 22
Sample_contact_city	Muenchen
Sample_contact_zip/postal_code	81675
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM292nnn/GSM292050/suppl/GSM292050.CEL.gz
Sample_series_id	GSE11589
Sample_data_row_count	45101

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