Search results for the GEO ID: GSE11628  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM294970 | GPL1261 |
|
D3-ES 7d with LIF, biological rep1
|
D3-ES cultivated with LIF for 7 days
|
mice embryonic stem cells
D3-ES cell line
|
Gene expression data from D3-ES cultivated with LIF for 7 days
|
| Sample_geo_accession | GSM294970
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Jun 01 2008
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | ATCC (LGC Promochem)
| | Sample_treatment_protocol_ch1 | Cells were trypsinized (10%, Gibco) and washed twice with PBS 1X and placed on ice. Total RNA was extracted with the Rneasy kit protocol (Qiagen).
| | Sample_growth_protocol_ch1 | 7 days of treatment with standard ESC medium (15% serum) with or without 1000 unit/ml LIF and 50 nM of lithium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy mini kit (Qiagen) of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA One-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 420 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were realized with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings from GeneChip® Operating System 1.4.0.036 and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patricia,,Ruiz-Ontañón
| | Sample_contact_email | patricia.ruiz@cabimer.es
| | Sample_contact_phone | 0034954467993
| | Sample_contact_department | TErapia CElular y Medicina Regenerativa
| | Sample_contact_institute | CABIMER
| | Sample_contact_address | Av Américo Vespucio
| | Sample_contact_city | Sevilla
| | Sample_contact_zip/postal_code | 41002
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294970/suppl/GSM294970.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294970/suppl/GSM294970.CHP.gz
| | Sample_series_id | GSE11628
| | Sample_data_row_count | 45101
| | |
|
GSM294971 | GPL1261 |
|
D3-ES 7d with LIF, biological rep2
|
D3-ES cultivated with LIF for 7 days
|
mice embryonic stem cells.
D3-ES cell line
|
Gene expression data from D3-ES cultivated with LIF for 7 days
|
| Sample_geo_accession | GSM294971
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Jun 01 2008
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | ATCC (LGC Promochem)
| | Sample_treatment_protocol_ch1 | Cells were trypsinized (10%, Gibco) and washed twice with PBS 1X and placed on ice. Total RNA was extracted with the Rneasy kit protocol (Qiagen).
| | Sample_growth_protocol_ch1 | 7 days of treatment with standard ESC medium (15% serum) with or without 1000 unit/ml LIF and 50 nM of lithium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy mini kit (Qiagen) of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA One-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 420 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were realized with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings from GeneChip® Operating System 1.4.0.036 and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patricia,,Ruiz-Ontañón
| | Sample_contact_email | patricia.ruiz@cabimer.es
| | Sample_contact_phone | 0034954467993
| | Sample_contact_department | TErapia CElular y Medicina Regenerativa
| | Sample_contact_institute | CABIMER
| | Sample_contact_address | Av Américo Vespucio
| | Sample_contact_city | Sevilla
| | Sample_contact_zip/postal_code | 41002
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294971/suppl/GSM294971.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294971/suppl/GSM294971.CHP.gz
| | Sample_series_id | GSE11628
| | Sample_data_row_count | 45101
| | |
|
GSM294972 | GPL1261 |
|
D3-ES 7d with LIF, biological rep3
|
D3-ES cultivated with LIF for 7 days
|
mice embryonic stem cells.
D3-ES cell line
|
Gene expression data from D3-ES cultivated with LIF for 7 days
|
| Sample_geo_accession | GSM294972
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Jun 01 2008
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | ATCC (LGC Promochem)
| | Sample_treatment_protocol_ch1 | Cells were trypsinized (10%, Gibco) and washed twice with PBS 1X and placed on ice. Total RNA was extracted with the Rneasy kit protocol (Qiagen).
| | Sample_growth_protocol_ch1 | 7 days of treatment with standard ESC medium (15% serum) with or without 1000 unit/ml LIF and 50 nM of lithium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy mini kit (Qiagen) of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA One-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 420 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were realized with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings from GeneChip® Operating System 1.4.0.036 and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patricia,,Ruiz-Ontañón
| | Sample_contact_email | patricia.ruiz@cabimer.es
| | Sample_contact_phone | 0034954467993
| | Sample_contact_department | TErapia CElular y Medicina Regenerativa
| | Sample_contact_institute | CABIMER
| | Sample_contact_address | Av Américo Vespucio
| | Sample_contact_city | Sevilla
| | Sample_contact_zip/postal_code | 41002
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294972/suppl/GSM294972.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294972/suppl/GSM294972.CHP.gz
| | Sample_series_id | GSE11628
| | Sample_data_row_count | 45101
| | |
|
GSM294973 | GPL1261 |
|
D3-ES 7d with LIF& 50 nM Li, biological rep1
|
D3-ES cultivated with LIF and treated with 50 nM lithium for 7 days
|
mice embryonic stem cells.
D3-ES cell line
|
Gene expression data from D3-ES cultivated with LIF and treated with 50 nM lithium for 7 days
|
| Sample_geo_accession | GSM294973
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Jun 01 2008
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | ATCC (LGC Promochem)
| | Sample_treatment_protocol_ch1 | Cells were trypsinized (10%, Gibco) and washed twice with PBS 1X and placed on ice. Total RNA was extracted with the Rneasy kit protocol (Qiagen).
| | Sample_growth_protocol_ch1 | 7 days of treatment with standard ESC medium (15% serum) with or without 1000 unit/ml LIF and 50 nM of lithium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy mini kit (Qiagen) of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA One-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 420 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were realized with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings from GeneChip® Operating System 1.4.0.036 and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patricia,,Ruiz-Ontañón
| | Sample_contact_email | patricia.ruiz@cabimer.es
| | Sample_contact_phone | 0034954467993
| | Sample_contact_department | TErapia CElular y Medicina Regenerativa
| | Sample_contact_institute | CABIMER
| | Sample_contact_address | Av Américo Vespucio
| | Sample_contact_city | Sevilla
| | Sample_contact_zip/postal_code | 41002
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294973/suppl/GSM294973.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294973/suppl/GSM294973.CHP.gz
| | Sample_series_id | GSE11628
| | Sample_data_row_count | 45101
| | |
|
GSM294974 | GPL1261 |
|
D3-ES 7d with LIF& 50 nM Li, biological rep2
|
D3-ES cultivated with LIF and treated with 50 nM lithium for 7 days
|
mice embryonic stem cells.
D3-ES cell line
|
Gene expression data from D3-ES cultivated with LIF and treated with 50 nM lithium for 7 days
|
| Sample_geo_accession | GSM294974
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Jun 01 2008
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | ATCC (LGC Promochem)
| | Sample_treatment_protocol_ch1 | Cells were trypsinized (10%, Gibco) and washed twice with PBS 1X and placed on ice. Total RNA was extracted with the Rneasy kit protocol (Qiagen).
| | Sample_growth_protocol_ch1 | 7 days of treatment with standard ESC medium (15% serum) with or without 1000 unit/ml LIF and 50 nM of lithium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy mini kit (Qiagen) of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA One-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 420 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were realized with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings from GeneChip® Operating System 1.4.0.036 and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patricia,,Ruiz-Ontañón
| | Sample_contact_email | patricia.ruiz@cabimer.es
| | Sample_contact_phone | 0034954467993
| | Sample_contact_department | TErapia CElular y Medicina Regenerativa
| | Sample_contact_institute | CABIMER
| | Sample_contact_address | Av Américo Vespucio
| | Sample_contact_city | Sevilla
| | Sample_contact_zip/postal_code | 41002
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294974/suppl/GSM294974.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294974/suppl/GSM294974.CHP.gz
| | Sample_series_id | GSE11628
| | Sample_data_row_count | 45101
| | |
|
GSM294975 | GPL1261 |
|
D3-ES 7d with LIF& 50 nM Li, biological rep3
|
D3-ES cultivated with LIF and treated with 50 nM lithium for 7 days
|
mice embryonic stem cells.
D3-ES cell line
|
Gene expression data from D3-ES cultivated with LIF and treated with 50 nM lithium for 7 days
|
| Sample_geo_accession | GSM294975
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Jun 01 2008
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | ATCC (LGC Promochem)
| | Sample_treatment_protocol_ch1 | Cells were trypsinized (10%, Gibco) and washed twice with PBS 1X and placed on ice. Total RNA was extracted with the Rneasy kit protocol (Qiagen).
| | Sample_growth_protocol_ch1 | 7 days of treatment with standard ESC medium (15% serum) with or without 1000 unit/ml LIF and 50 nM of lithium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy mini kit (Qiagen) of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA One-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 420 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were realized with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings from GeneChip® Operating System 1.4.0.036 and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patricia,,Ruiz-Ontañón
| | Sample_contact_email | patricia.ruiz@cabimer.es
| | Sample_contact_phone | 0034954467993
| | Sample_contact_department | TErapia CElular y Medicina Regenerativa
| | Sample_contact_institute | CABIMER
| | Sample_contact_address | Av Américo Vespucio
| | Sample_contact_city | Sevilla
| | Sample_contact_zip/postal_code | 41002
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294975/suppl/GSM294975.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294975/suppl/GSM294975.CHP.gz
| | Sample_series_id | GSE11628
| | Sample_data_row_count | 45101
| | |
|
GSM294976 | GPL1261 |
|
D3-ES 7d without LIF, biological rep1
|
D3-ES cultivated without LIF for 7 days
|
mice embryonic stem cells.
D3-ES cell line
|
Gene expression data from D3-ES cultivated without LIF for 7 days
|
| Sample_geo_accession | GSM294976
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Jun 01 2008
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | ATCC (LGC Promochem)
| | Sample_treatment_protocol_ch1 | Cells were trypsinized (10%, Gibco) and washed twice with PBS 1X and placed on ice. Total RNA was extracted with the Rneasy kit protocol (Qiagen).
| | Sample_growth_protocol_ch1 | 7 days of treatment with standard ESC medium (15% serum) with or without 1000 unit/ml LIF and 50 nM of lithium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy mini kit (Qiagen) of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA One-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 420 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were realized with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings from GeneChip® Operating System 1.4.0.036 and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patricia,,Ruiz-Ontañón
| | Sample_contact_email | patricia.ruiz@cabimer.es
| | Sample_contact_phone | 0034954467993
| | Sample_contact_department | TErapia CElular y Medicina Regenerativa
| | Sample_contact_institute | CABIMER
| | Sample_contact_address | Av Américo Vespucio
| | Sample_contact_city | Sevilla
| | Sample_contact_zip/postal_code | 41002
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294976/suppl/GSM294976.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294976/suppl/GSM294976.CHP.gz
| | Sample_series_id | GSE11628
| | Sample_data_row_count | 45101
| | |
|
GSM294977 | GPL1261 |
|
D3-ES 7d without LIF, biological rep2
|
D3-ES cultivated without LIF for 7 days
|
mice embryonic stem cells.
D3-ES cell line
|
Gene expression data from D3-ES cultivated without LIF for 7 days
|
| Sample_geo_accession | GSM294977
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Jun 01 2008
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | ATCC (LGC Promochem)
| | Sample_treatment_protocol_ch1 | Cells were trypsinized (10%, Gibco) and washed twice with PBS 1X and placed on ice. Total RNA was extracted with the Rneasy kit protocol (Qiagen).
| | Sample_growth_protocol_ch1 | 7 days of treatment with standard ESC medium (15% serum) with or without 1000 unit/ml LIF and 50 nM of lithium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy mini kit (Qiagen) of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA One-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 420 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were realized with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings from GeneChip® Operating System 1.4.0.036 and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patricia,,Ruiz-Ontañón
| | Sample_contact_email | patricia.ruiz@cabimer.es
| | Sample_contact_phone | 0034954467993
| | Sample_contact_department | TErapia CElular y Medicina Regenerativa
| | Sample_contact_institute | CABIMER
| | Sample_contact_address | Av Américo Vespucio
| | Sample_contact_city | Sevilla
| | Sample_contact_zip/postal_code | 41002
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294977/suppl/GSM294977.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294977/suppl/GSM294977.CHP.gz
| | Sample_series_id | GSE11628
| | Sample_data_row_count | 45101
| | |
|
GSM294978 | GPL1261 |
|
D3-ES 7d without LIF, biological rep3
|
D3-ES cultivated without LIF for 7 days
|
mice embryonic stem cells.
D3-ES cell line
|
Gene expression data from D3-ES cultivated without LIF for 7 days
|
| Sample_geo_accession | GSM294978
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Jun 01 2008
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | ATCC (LGC Promochem)
| | Sample_treatment_protocol_ch1 | Cells were trypsinized (10%, Gibco) and washed twice with PBS 1X and placed on ice. Total RNA was extracted with the Rneasy kit protocol (Qiagen).
| | Sample_growth_protocol_ch1 | 7 days of treatment with standard ESC medium (15% serum) with or without 1000 unit/ml LIF and 50 nM of lithium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy mini kit (Qiagen) of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA One-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 420 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were realized with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings from GeneChip® Operating System 1.4.0.036 and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patricia,,Ruiz-Ontañón
| | Sample_contact_email | patricia.ruiz@cabimer.es
| | Sample_contact_phone | 0034954467993
| | Sample_contact_department | TErapia CElular y Medicina Regenerativa
| | Sample_contact_institute | CABIMER
| | Sample_contact_address | Av Américo Vespucio
| | Sample_contact_city | Sevilla
| | Sample_contact_zip/postal_code | 41002
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294978/suppl/GSM294978.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294978/suppl/GSM294978.CHP.gz
| | Sample_series_id | GSE11628
| | Sample_data_row_count | 45101
| | |
|
GSM294979 | GPL1261 |
|
D3-ES 7d without LIF& 50 nM Li, biological rep1
|
D3-ES cultivated without LIF and treated with 50 nM lithum for 7 days
|
mice embryonic stem cells.
D3-ES cell line
|
Gene expression data from D3-ES cultivated without LIF and treated with 50 nM lithium for 7 days
|
| Sample_geo_accession | GSM294979
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Jun 01 2008
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | ATCC (LGC Promochem)
| | Sample_treatment_protocol_ch1 | Cells were trypsinized (10%, Gibco) and washed twice with PBS 1X and placed on ice. Total RNA was extracted with the Rneasy kit protocol (Qiagen).
| | Sample_growth_protocol_ch1 | 7 days of treatment with standard ESC medium (15% serum) with or without 1000 unit/ml LIF and 50 nM of lithium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy mini kit (Qiagen) of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA One-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 420 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were realized with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings from GeneChip® Operating System 1.4.0.036 and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patricia,,Ruiz-Ontañón
| | Sample_contact_email | patricia.ruiz@cabimer.es
| | Sample_contact_phone | 0034954467993
| | Sample_contact_department | TErapia CElular y Medicina Regenerativa
| | Sample_contact_institute | CABIMER
| | Sample_contact_address | Av Américo Vespucio
| | Sample_contact_city | Sevilla
| | Sample_contact_zip/postal_code | 41002
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294979/suppl/GSM294979.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294979/suppl/GSM294979.CHP.gz
| | Sample_series_id | GSE11628
| | Sample_data_row_count | 45101
| | |
|
GSM294980 | GPL1261 |
|
D3-ES 7d without LIF& 50 nM Li, biological rep2
|
D3-ES cultivated without LIF and treated with 50 nM lithum for 7 days
|
mice embryonic stem cells.
D3-ES cell line
|
Gene expression data from D3-ES cultivated without LIF and treated with 50 nM lithium for 7 days
|
| Sample_geo_accession | GSM294980
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Jun 01 2008
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | ATCC (LGC Promochem)
| | Sample_treatment_protocol_ch1 | Cells were trypsinized (10%, Gibco) and washed twice with PBS 1X and placed on ice. Total RNA was extracted with the Rneasy kit protocol (Qiagen).
| | Sample_growth_protocol_ch1 | 7 days of treatment with standard ESC medium (15% serum) with or without 1000 unit/ml LIF and 50 nM of lithium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy mini kit (Qiagen) of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA One-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 420 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were realized with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings from GeneChip® Operating System 1.4.0.036 and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patricia,,Ruiz-Ontañón
| | Sample_contact_email | patricia.ruiz@cabimer.es
| | Sample_contact_phone | 0034954467993
| | Sample_contact_department | TErapia CElular y Medicina Regenerativa
| | Sample_contact_institute | CABIMER
| | Sample_contact_address | Av Américo Vespucio
| | Sample_contact_city | Sevilla
| | Sample_contact_zip/postal_code | 41002
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294980/suppl/GSM294980.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294980/suppl/GSM294980.CHP.gz
| | Sample_series_id | GSE11628
| | Sample_data_row_count | 45101
| | |
|
GSM294981 | GPL1261 |
|
D3-ES 7d without LIF& 50 nM Li, biological rep3
|
D3-ES cultivated without LIF and treated with 50 nM lithum for 7 days
|
mice embryonic stem cells.
D3-ES cell line
|
Gene expression data from D3-ES cultivated without LIF and treated with 50 nM lithium for 7 days
|
| Sample_geo_accession | GSM294981
| | Sample_status | Public on Dec 31 2009
| | Sample_submission_date | Jun 01 2008
| | Sample_last_update_date | Apr 29 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | ATCC (LGC Promochem)
| | Sample_treatment_protocol_ch1 | Cells were trypsinized (10%, Gibco) and washed twice with PBS 1X and placed on ice. Total RNA was extracted with the Rneasy kit protocol (Qiagen).
| | Sample_growth_protocol_ch1 | 7 days of treatment with standard ESC medium (15% serum) with or without 1000 unit/ml LIF and 50 nM of lithium
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy mini kit (Qiagen) of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA One-Cycle Target Labeling Assays (GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 420 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression AnalysisTechnical Manual, Affymetrix, 2004.
| | Sample_scan_protocol | Microarrays were scanned using GeneChip® Scanner 3000 7G
| | Sample_data_processing | The data were realized with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings from GeneChip® Operating System 1.4.0.036 and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Patricia,,Ruiz-Ontañón
| | Sample_contact_email | patricia.ruiz@cabimer.es
| | Sample_contact_phone | 0034954467993
| | Sample_contact_department | TErapia CElular y Medicina Regenerativa
| | Sample_contact_institute | CABIMER
| | Sample_contact_address | Av Américo Vespucio
| | Sample_contact_city | Sevilla
| | Sample_contact_zip/postal_code | 41002
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294981/suppl/GSM294981.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM294nnn/GSM294981/suppl/GSM294981.CHP.gz
| | Sample_series_id | GSE11628
| | Sample_data_row_count | 45101
| | |
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