Search results for the GEO ID: GSE11659 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM296193 | GPL339 |
|
BXH10-1
|
BXH10-1 Lung
|
1 tissue: Lung
|
BXH10-1 Lung gene expression
|
Sample_geo_accession | GSM296193
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296193/suppl/GSM296193.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296194 | GPL339 |
|
BXH10-2
|
BXH10-2 Lung
|
1 tissue: Lung
|
BXH10-2 Lung gene expression
|
Sample_geo_accession | GSM296194
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296194/suppl/GSM296194.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296195 | GPL339 |
|
BXH14-1
|
BXH14-1 Lung
|
1 tissue: Lung
|
BXH14-1 Lung gene expression
|
Sample_geo_accession | GSM296195
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296195/suppl/GSM296195.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296196 | GPL339 |
|
BXH14-2
|
BXH14-2 Lung
|
1 tissue: Lung
|
BXH14-2 Lung gene expression
|
Sample_geo_accession | GSM296196
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296196/suppl/GSM296196.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296197 | GPL339 |
|
BXH19-1
|
BXH19-1 Lung
|
1 tissue: Lung
|
BXH19-1 Lung gene expression
|
Sample_geo_accession | GSM296197
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296197/suppl/GSM296197.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296198 | GPL339 |
|
BXH19-2
|
BXH19-2 Lung
|
1 tissue: Lung
|
BXH19-2 Lung gene expression
|
Sample_geo_accession | GSM296198
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296198/suppl/GSM296198.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296199 | GPL339 |
|
BXH2-1
|
BXH2-1 Lung
|
1 tissue: Lung
|
BXH2-1 Lung gene expression
|
Sample_geo_accession | GSM296199
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296199/suppl/GSM296199.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296200 | GPL339 |
|
BXH2-2
|
BXH2-2 Lung
|
1 tissue: Lung
|
BXH2-2 Lung gene expression
|
Sample_geo_accession | GSM296200
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296200/suppl/GSM296200.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296201 | GPL339 |
|
BXH4-1
|
BXH4-1 Lung
|
1 tissue: Lung
|
BXH4-1 Lung gene expression
|
Sample_geo_accession | GSM296201
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296201/suppl/GSM296201.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296202 | GPL339 |
|
BXH4-2
|
BXH4-2 Lung
|
1 tissue: Lung
|
BXH4-2 Lung gene expression
|
Sample_geo_accession | GSM296202
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296202/suppl/GSM296202.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296203 | GPL339 |
|
BXH7-1
|
BXH7-1 Lung
|
1 tissue: Lung
|
BXH7-1 Lung gene expression
|
Sample_geo_accession | GSM296203
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296203/suppl/GSM296203.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296204 | GPL339 |
|
BXH7-2
|
BXH7-2 Lung
|
1 tissue: Lung
|
BXH7-2 Lung gene expression
|
Sample_geo_accession | GSM296204
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296204/suppl/GSM296204.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296205 | GPL339 |
|
BXH8-1
|
BXH8-1 Lung
|
1 tissue: Lung
|
BXH8-1 Lung gene expression
|
Sample_geo_accession | GSM296205
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296205/suppl/GSM296205.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296206 | GPL339 |
|
BXH8-2
|
BXH8-2 Lung
|
1 tissue: Lung
|
BXH8-2 Lung gene expression
|
Sample_geo_accession | GSM296206
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296206/suppl/GSM296206.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296207 | GPL339 |
|
BXH9-1
|
BXH9-1 Lung
|
1 tissue: Lung
|
BXH9-1 Lung gene expression
|
Sample_geo_accession | GSM296207
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296207/suppl/GSM296207.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296208 | GPL339 |
|
BXH9-2
|
BXH9-2 Lung
|
1 tissue: Lung
|
BXH9-2 Lung gene expression
|
Sample_geo_accession | GSM296208
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296208/suppl/GSM296208.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296209 | GPL339 |
|
BXH6-1
|
BXH6-1 Lung
|
1 tissue: Lung
|
BXH6-1 Lung gene expression
|
Sample_geo_accession | GSM296209
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296209/suppl/GSM296209.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296210 | GPL339 |
|
BXH6-2
|
BXH6-2 Lung
|
1 tissue: Lung
|
BXH6-2 Lung gene expression
|
Sample_geo_accession | GSM296210
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296210/suppl/GSM296210.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296211 | GPL339 |
|
B6-1
|
B6-1 Lung
|
1 tissue: Lung
|
B6-1 Lung gene expression
|
Sample_geo_accession | GSM296211
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296211/suppl/GSM296211.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296212 | GPL339 |
|
B6-2
|
B6-2 Lung
|
1 tissue: Lung
|
B6-2 Lung gene expression
|
Sample_geo_accession | GSM296212
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296212/suppl/GSM296212.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296213 | GPL339 |
|
C3-1
|
C3-1 Lung
|
1 tissue: Lung
|
C3-1 Lung gene expression
|
Sample_geo_accession | GSM296213
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296213/suppl/GSM296213.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296214 | GPL339 |
|
C3-2
|
C3-2 Lung
|
1 tissue: Lung
|
C3-2 Lung gene expression
|
Sample_geo_accession | GSM296214
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296214/suppl/GSM296214.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296215 | GPL339 |
|
F1-1
|
F1-1 Lung
|
1 tissue: Lung
|
F1-1 Lung gene expression
|
Sample_geo_accession | GSM296215
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296215/suppl/GSM296215.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
GSM296216 | GPL339 |
|
F1-2
|
F1-2 Lung
|
1 tissue: Lung
|
F1-2 Lung gene expression
|
Sample_geo_accession | GSM296216
| Sample_status | Public on Nov 26 2011
| Sample_submission_date | Jun 03 2008
| Sample_last_update_date | Nov 26 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | no treatment
| Sample_growth_protocol_ch1 | no growth protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA step using Invitrogen SuperScript Double-stranded cDNA Synthesis Kit cDNA cleanup by Affymetrix GeneChip Sample Cleanup Module,cRNA amplification by Ambion MEGAscript T7 Kit, Cleanup by Qiagen RNeasy Mini Kit. cDNA synthesis by Invitrogen SuperScript Double-stranded cDNA Synthesis Kit using a T7-Oligo(dT) promoter primer from Affymetrix, cRNA amplification using ENZO BioArray HighYield RNA Transcript Labeling Kit, Metal-induced hydrolysis by Affymetrix 5 X Fragmentation Buffer, External controls (spikes)- Control Oligo B2 (3 nM), Eukaryotic Hybridization control (Affymetrix) (Small Sample Labeling Protocol vII, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 1 200 ul of hybridization cocktail containing 10 ug of fragmented cRNA placed on chip - Hybridized for 18 hours in GeneChip Hybridization Oven 640 - Washed and stained using 2 strepavidin stains and one antibody stain on GeneChip Fluidics Station 400 for 1.5 hours (GeneChip Expression Analysis Technical Manual- Affymetrix)
| Sample_scan_protocol | Arrays were scanned with a GCS300 Scanner (Affymetrix) for images and intensities.
| Sample_data_processing | Data extraction was processed by Affy GeneChip® Operating Software v1.3 (Affymetrix). Global scaling was equal to 500. Normalization factor was equal to 1.
| Sample_platform_id | GPL339
| Sample_contact_name | vikas,,misra
| Sample_contact_email | vmisra@jhsph.edu
| Sample_contact_laboratory | Clarke G Tankersley
| Sample_contact_department | Environmental health sciences
| Sample_contact_institute | Johns Hopkins University School of public health
| Sample_contact_address | 615 N. Wolfe street, E6609
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296216/suppl/GSM296216.CEL.gz
| Sample_series_id | GSE11659
| Sample_data_row_count | 22690
| |
|
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