Result

Search results for the GEO ID: GSE11677

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Title

Source name

Description

Characteristics

GSM296650

GPL1261

CD8 memory T cell_Expansion_rep1

CD8 clonal expansion

Mouse strain: C57BL/10SnJ; Gender: Female; Age: 20 months; Tissue: Spleen and lymph nodes; Purified by protein expression: CD8+ CD122+ TCR Vb3+ MHC II-; Cell Purity: 91%

Expansion_rep1 belongs to the integrin a4 high subclass of clonal expansions. This type of clonal expansion is very different than the integrin a4 low type of clonal expansion (represented by samples 2, 4, and 5).

Sample_geo_accession	GSM296650
Sample_status	Public on Aug 15 2008
Sample_submission_date	Jun 04 2008
Sample_last_update_date	Aug 04 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	CD8 memory T cells were purified by flow cytometric sorting directly from aged mice. In order to purify these cells, samples were stained with a variety of fluorescently labelled antibodies that bound to cell surface molecules and sorted by use of a flow cytometer. Once cells were purified, they were immediately frozen on crushed dry ice and stored at -80 C until time of RNA extraction.
Sample_growth_protocol_ch1	Cells were not subjected to any in vitro growth conditions. Cells were directly frozen following their purification from mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was purified from all samples in parallel using the PicoPure RNA Isolation Kit (Arcturus, Mountain View, California). To obtain enough RNA for microarray analysis, all samples were subjected to double RNA amplification using the RiboAmp OA RNA amplification kit (Arcturus).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Amplified RNA samples were labeled with biotin using the Affymetrix GeneChip IVT labeling kit (Santa Clara, California).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix (GeneChip) Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000(type M10).
Sample_data_processing	The data were analyzed with Affymetrix GeneChip Operating Software version 1 (MAS 5 statistical algorithm) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Eric,,Clambey
Sample_contact_email	etclambe@hotmail.com
Sample_contact_laboratory	Philippa Marrack & John Kappler
Sample_contact_department	Immunology
Sample_contact_institute	National Jewish Research & Medical Center
Sample_contact_address	1400 Jackson Street, Goodman Bldg, Rm K519
Sample_contact_city	Denver
Sample_contact_state	CO
Sample_contact_zip/postal_code	80206
Sample_contact_country	USA
Sample_contact_web_link	http://www.kmlab.njc.org/
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296650/suppl/GSM296650.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296650/suppl/GSM296650.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296650/suppl/GSM296650.EXP.gz
Sample_series_id	GSE11677
Sample_data_row_count	45101

GSM296651

GPL1261

CD8 memory T cell_PolyclonalAged_rep1

Polyclonal, aged CD8 memory T cells

Mouse strain: C57BL/10SnJ; Gender: Female; Age: 20 months; Tissue: Spleen and lymph nodes; Purified by protein expression: CD8+ CD122+ TCR Vb3- MHC II-; Cell Purity: 97%

CD8 memory T cell_PolyclonalAged_rep1

Sample_geo_accession	GSM296651
Sample_status	Public on Aug 15 2008
Sample_submission_date	Jun 04 2008
Sample_last_update_date	Aug 04 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	CD8 memory T cells were purified by flow cytometric sorting directly from aged mice. In order to purify these cells, samples were stained with a variety of fluorescently labelled antibodies that bound to cell surface molecules and sorted by use of a flow cytometer. Once cells were purified, they were immediately frozen on crushed dry ice and stored at -80 C until time of RNA extraction.
Sample_growth_protocol_ch1	Cells were not subjected to any in vitro growth conditions. Cells were directly frozen following their purification from mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was purified from all samples in parallel using the PicoPure RNA Isolation Kit (Arcturus, Mountain View, California). To obtain enough RNA for microarray analysis, all samples were subjected to double RNA amplification using the RiboAmp OA RNA amplification kit (Arcturus).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Amplified RNA samples were labeled with biotin using the Affymetrix GeneChip IVT labeling kit (Santa Clara, California).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix (GeneChip) Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000(type M10).
Sample_data_processing	The data were analyzed with Affymetrix GeneChip Operating Software version 1 (MAS 5 statistical algorithm) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Eric,,Clambey
Sample_contact_email	etclambe@hotmail.com
Sample_contact_laboratory	Philippa Marrack & John Kappler
Sample_contact_department	Immunology
Sample_contact_institute	National Jewish Research & Medical Center
Sample_contact_address	1400 Jackson Street, Goodman Bldg, Rm K519
Sample_contact_city	Denver
Sample_contact_state	CO
Sample_contact_zip/postal_code	80206
Sample_contact_country	USA
Sample_contact_web_link	http://www.kmlab.njc.org/
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296651/suppl/GSM296651.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296651/suppl/GSM296651.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296651/suppl/GSM296651.EXP.gz
Sample_series_id	GSE11677
Sample_data_row_count	45101

GSM296652

GPL1261

CD8 memory T cell_Expansion_rep2

CD8 clonal expansion

Mouse strain: C57BL/10SnJ; Gender: Female; Age: 20 months; Tissue: Spleen and lymph nodes; Purified by protein expression: CD8+ CD122+ TCR Vb8.1/8.2+ MHC II-; Cell Purity: 95%

Expansion_rep2 belongs to the integrin a4 LOW subclass of clonal expansions.

Sample_geo_accession	GSM296652
Sample_status	Public on Aug 15 2008
Sample_submission_date	Jun 04 2008
Sample_last_update_date	Aug 04 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	CD8 memory T cells were purified by flow cytometric sorting directly from aged mice. In order to purify these cells, samples were stained with a variety of fluorescently labelled antibodies that bound to cell surface molecules and sorted by use of a flow cytometer. Once cells were purified, they were immediately frozen on crushed dry ice and stored at -80 C until time of RNA extraction.
Sample_growth_protocol_ch1	Cells were not subjected to any in vitro growth conditions. Cells were directly frozen following their purification from mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was purified from all samples in parallel using the PicoPure RNA Isolation Kit (Arcturus, Mountain View, California). To obtain enough RNA for microarray analysis, all samples were subjected to double RNA amplification using the RiboAmp OA RNA amplification kit (Arcturus).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Amplified RNA samples were labeled with biotin using the Affymetrix GeneChip IVT labeling kit (Santa Clara, California).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix (GeneChip) Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000(type M10).
Sample_data_processing	The data were analyzed with Affymetrix GeneChip Operating Software version 1 (MAS 5 statistical algorithm) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Eric,,Clambey
Sample_contact_email	etclambe@hotmail.com
Sample_contact_laboratory	Philippa Marrack & John Kappler
Sample_contact_department	Immunology
Sample_contact_institute	National Jewish Research & Medical Center
Sample_contact_address	1400 Jackson Street, Goodman Bldg, Rm K519
Sample_contact_city	Denver
Sample_contact_state	CO
Sample_contact_zip/postal_code	80206
Sample_contact_country	USA
Sample_contact_web_link	http://www.kmlab.njc.org/
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296652/suppl/GSM296652.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296652/suppl/GSM296652.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296652/suppl/GSM296652.EXP.gz
Sample_series_id	GSE11677
Sample_data_row_count	45101

GSM296653

GPL1261

CD8 memory T cell_PolyclonalAged_rep2

Polyclonal, aged CD8 memory T cells

Mouse strain: C57BL/10SnJ; Gender: Female; Age: 20 months; Tissue: Spleen and lymph nodes; Purified by protein expression: CD8+ CD122+ TCR Vb8.1/8.2- MHC II-; Cell Purity: 75%

CD8 memory T cell_PolyclonalAged_rep2

Sample_geo_accession	GSM296653
Sample_status	Public on Aug 15 2008
Sample_submission_date	Jun 04 2008
Sample_last_update_date	Aug 04 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	CD8 memory T cells were purified by flow cytometric sorting directly from aged mice. In order to purify these cells, samples were stained with a variety of fluorescently labelled antibodies that bound to cell surface molecules and sorted by use of a flow cytometer. Once cells were purified, they were immediately frozen on crushed dry ice and stored at -80 C until time of RNA extraction.
Sample_growth_protocol_ch1	Cells were not subjected to any in vitro growth conditions. Cells were directly frozen following their purification from mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was purified from all samples in parallel using the PicoPure RNA Isolation Kit (Arcturus, Mountain View, California). To obtain enough RNA for microarray analysis, all samples were subjected to double RNA amplification using the RiboAmp OA RNA amplification kit (Arcturus).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Amplified RNA samples were labeled with biotin using the Affymetrix GeneChip IVT labeling kit (Santa Clara, California).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix (GeneChip) Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000(type M10).
Sample_data_processing	The data were analyzed with Affymetrix GeneChip Operating Software version 1 (MAS 5 statistical algorithm) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Eric,,Clambey
Sample_contact_email	etclambe@hotmail.com
Sample_contact_laboratory	Philippa Marrack & John Kappler
Sample_contact_department	Immunology
Sample_contact_institute	National Jewish Research & Medical Center
Sample_contact_address	1400 Jackson Street, Goodman Bldg, Rm K519
Sample_contact_city	Denver
Sample_contact_state	CO
Sample_contact_zip/postal_code	80206
Sample_contact_country	USA
Sample_contact_web_link	http://www.kmlab.njc.org/
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296653/suppl/GSM296653.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296653/suppl/GSM296653.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296653/suppl/GSM296653.EXP.gz
Sample_series_id	GSE11677
Sample_data_row_count	45101

GSM296654

GPL1261

CD8 memory T cell_Expansion_rep3

CD8 clonal expansion

Mouse strain: C57BL/10SnJ; Gender: Female; Age: 23 months; Tissue: Spleen and lymph nodes; Purified by protein expression: CD8+ CD122+ TCR Vb8.3+ MHC II-; Cell Purity: 96%

Expansion_rep4 belongs to the integrin a4 LOW subclass of clonal expansions.

Sample_geo_accession	GSM296654
Sample_status	Public on Aug 15 2008
Sample_submission_date	Jun 04 2008
Sample_last_update_date	Aug 04 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	CD8 memory T cells were purified by flow cytometric sorting directly from aged mice. In order to purify these cells, samples were stained with a variety of fluorescently labelled antibodies that bound to cell surface molecules and sorted by use of a flow cytometer. Once cells were purified, they were immediately frozen on crushed dry ice and stored at -80 C until time of RNA extraction.
Sample_growth_protocol_ch1	Cells were not subjected to any in vitro growth conditions. Cells were directly frozen following their purification from mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was purified from all samples in parallel using the PicoPure RNA Isolation Kit (Arcturus, Mountain View, California). To obtain enough RNA for microarray analysis, all samples were subjected to double RNA amplification using the RiboAmp OA RNA amplification kit (Arcturus).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Amplified RNA samples were labeled with biotin using the Affymetrix GeneChip IVT labeling kit (Santa Clara, California).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix (GeneChip) Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000(type M10).
Sample_data_processing	The data were analyzed with Affymetrix GeneChip Operating Software version 1 (MAS 5 statistical algorithm) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Eric,,Clambey
Sample_contact_email	etclambe@hotmail.com
Sample_contact_laboratory	Philippa Marrack & John Kappler
Sample_contact_department	Immunology
Sample_contact_institute	National Jewish Research & Medical Center
Sample_contact_address	1400 Jackson Street, Goodman Bldg, Rm K519
Sample_contact_city	Denver
Sample_contact_state	CO
Sample_contact_zip/postal_code	80206
Sample_contact_country	USA
Sample_contact_web_link	http://www.kmlab.njc.org/
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296654/suppl/GSM296654.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296654/suppl/GSM296654.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296654/suppl/GSM296654.EXP.gz
Sample_series_id	GSE11677
Sample_data_row_count	45101

GSM296655

GPL1261

CD8 memory T cell_PolyclonalAged_rep3

Polyclonal, aged CD8 memory T cells

Mouse strain: C57BL/10SnJ; Gender: Female; Age: 23 months; Tissue: Spleen and lymph nodes; Purified by protein expression: CD8+ CD122+ TCR Vb8.3- MHC II-; Cell Purity: 66%

CD8 memory T cell_PolyclonalAged_rep3

Sample_geo_accession	GSM296655
Sample_status	Public on Aug 15 2008
Sample_submission_date	Jun 04 2008
Sample_last_update_date	Aug 04 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	CD8 memory T cells were purified by flow cytometric sorting directly from aged mice. In order to purify these cells, samples were stained with a variety of fluorescently labelled antibodies that bound to cell surface molecules and sorted by use of a flow cytometer. Once cells were purified, they were immediately frozen on crushed dry ice and stored at -80 C until time of RNA extraction.
Sample_growth_protocol_ch1	Cells were not subjected to any in vitro growth conditions. Cells were directly frozen following their purification from mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was purified from all samples in parallel using the PicoPure RNA Isolation Kit (Arcturus, Mountain View, California). To obtain enough RNA for microarray analysis, all samples were subjected to double RNA amplification using the RiboAmp OA RNA amplification kit (Arcturus).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Amplified RNA samples were labeled with biotin using the Affymetrix GeneChip IVT labeling kit (Santa Clara, California).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix (GeneChip) Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000(type M10).
Sample_data_processing	The data were analyzed with Affymetrix GeneChip Operating Software version 1 (MAS 5 statistical algorithm) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Eric,,Clambey
Sample_contact_email	etclambe@hotmail.com
Sample_contact_laboratory	Philippa Marrack & John Kappler
Sample_contact_department	Immunology
Sample_contact_institute	National Jewish Research & Medical Center
Sample_contact_address	1400 Jackson Street, Goodman Bldg, Rm K519
Sample_contact_city	Denver
Sample_contact_state	CO
Sample_contact_zip/postal_code	80206
Sample_contact_country	USA
Sample_contact_web_link	http://www.kmlab.njc.org/
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296655/suppl/GSM296655.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296655/suppl/GSM296655.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296655/suppl/GSM296655.EXP.gz
Sample_series_id	GSE11677
Sample_data_row_count	45101

GSM296656

GPL1261

CD8 memory T cell_Expansion_rep4

CD8 clonal expansion

Mouse strain: B10.BR; Gender: Female; Age: 23 months; Tissue: Spleen and lymph nodes; Purified by protein expression: CD8+ CD122+ TCR Vb14+ MHC II-; Cell Purity: 98%

Expansion_rep5 belongs to the integrin a4 LOW subclass of clonal expansions.

Sample_geo_accession	GSM296656
Sample_status	Public on Aug 15 2008
Sample_submission_date	Jun 04 2008
Sample_last_update_date	Aug 04 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	CD8 memory T cells were purified by flow cytometric sorting directly from aged mice. In order to purify these cells, samples were stained with a variety of fluorescently labelled antibodies that bound to cell surface molecules and sorted by use of a flow cytometer. Once cells were purified, they were immediately frozen on crushed dry ice and stored at -80 C until time of RNA extraction.
Sample_growth_protocol_ch1	Cells were not subjected to any in vitro growth conditions. Cells were directly frozen following their purification from mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was purified from all samples in parallel using the PicoPure RNA Isolation Kit (Arcturus, Mountain View, California). To obtain enough RNA for microarray analysis, all samples were subjected to double RNA amplification using the RiboAmp OA RNA amplification kit (Arcturus).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Amplified RNA samples were labeled with biotin using the Affymetrix GeneChip IVT labeling kit (Santa Clara, California).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix (GeneChip) Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000(type M10).
Sample_data_processing	The data were analyzed with Affymetrix GeneChip Operating Software version 1 (MAS 5 statistical algorithm) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Eric,,Clambey
Sample_contact_email	etclambe@hotmail.com
Sample_contact_laboratory	Philippa Marrack & John Kappler
Sample_contact_department	Immunology
Sample_contact_institute	National Jewish Research & Medical Center
Sample_contact_address	1400 Jackson Street, Goodman Bldg, Rm K519
Sample_contact_city	Denver
Sample_contact_state	CO
Sample_contact_zip/postal_code	80206
Sample_contact_country	USA
Sample_contact_web_link	http://www.kmlab.njc.org/
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296656/suppl/GSM296656.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296656/suppl/GSM296656.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296656/suppl/GSM296656.EXP.gz
Sample_series_id	GSE11677
Sample_data_row_count	45101

GSM296657

GPL1261

CD8 memory T cell_PolyclonalAged_rep4

Polyclonal, aged CD8 memory T cells

Mouse strain: B10.BR; Gender: Female; Age: 23 months; Tissue: Spleen and lymph nodes; Purified by protein expression: CD8+ CD122+ TCR Vb14- MHC II-; Cell Purity: 97%

CD8 memory T cell_PolyclonalAged_rep4

Sample_geo_accession	GSM296657
Sample_status	Public on Aug 15 2008
Sample_submission_date	Jun 04 2008
Sample_last_update_date	Aug 04 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	CD8 memory T cells were purified by flow cytometric sorting directly from aged mice. In order to purify these cells, samples were stained with a variety of fluorescently labelled antibodies that bound to cell surface molecules and sorted by use of a flow cytometer. Once cells were purified, they were immediately frozen on crushed dry ice and stored at -80 C until time of RNA extraction.
Sample_growth_protocol_ch1	Cells were not subjected to any in vitro growth conditions. Cells were directly frozen following their purification from mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was purified from all samples in parallel using the PicoPure RNA Isolation Kit (Arcturus, Mountain View, California). To obtain enough RNA for microarray analysis, all samples were subjected to double RNA amplification using the RiboAmp OA RNA amplification kit (Arcturus).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Amplified RNA samples were labeled with biotin using the Affymetrix GeneChip IVT labeling kit (Santa Clara, California).
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix (GeneChip) Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000(type M10).
Sample_data_processing	The data were analyzed with Affymetrix GeneChip Operating Software version 1 (MAS 5 statistical algorithm) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Eric,,Clambey
Sample_contact_email	etclambe@hotmail.com
Sample_contact_laboratory	Philippa Marrack & John Kappler
Sample_contact_department	Immunology
Sample_contact_institute	National Jewish Research & Medical Center
Sample_contact_address	1400 Jackson Street, Goodman Bldg, Rm K519
Sample_contact_city	Denver
Sample_contact_state	CO
Sample_contact_zip/postal_code	80206
Sample_contact_country	USA
Sample_contact_web_link	http://www.kmlab.njc.org/
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296657/suppl/GSM296657.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296657/suppl/GSM296657.CHP.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM296nnn/GSM296657/suppl/GSM296657.EXP.gz
Sample_series_id	GSE11677
Sample_data_row_count	45101

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