Search results for the GEO ID: GSE11791  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM298353 | GPL570 |
|
MCF7_EtOH_rep1
|
MCF-7, ICI 182780 pretreatment then treated with ethanol vehicle
|
MCF-7 human breast cancer cells
|
MCF-7 cells pretreated with ICI 182780 then treated with ethanol vehicle
|
| Sample_geo_accession | GSM298353
| | Sample_status | Public on Aug 21 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Aug 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Exponentially proliferating cells were growth arrested by pretreatment for 48 h with 10nM steroidal antiestrogen ICI 182780 and then treated with either 100nM E2 or 75 uM Zn (as ZnSO4). Vehicle controls for E2 or Zn were absolute ethanol and water respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from three independent experiments was isolated from cells using the RNeasy mini kit (Qiagen Pty Ltd, Clifton Hill, Vic Australia) following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fluorescent cRNA probes for Affymetrix oligonucleotide microarray analysis were generated using the One-cycle Target labelling and Control Reagent package (Millennium Science, Box Hill, Vic Australia) according to the manufacturer's instructions
| | Sample_hyb_protocol | Probes were hybridised at 45 C for 16 h and then washed and doubly stained using the GeneChip 400 fluidics station according to the manufacturer's instructions
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner (Affymetrix- Millennium Science, Surrey Hill, VIC, Australia)
| | Sample_data_processing | Signals obtained were analysed with Microarray Suite expression algorithm (version 5.0.1; Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Liz,,Musgrove
| | Sample_contact_email | e.musgrove@garvan.org.au
| | Sample_contact_phone | +61 2 9295 8328
| | Sample_contact_fax | +61 2 9295 8321
| | Sample_contact_department | Cancer Research Program
| | Sample_contact_institute | Garvan Institute of Medical Research
| | Sample_contact_address | 384 Victoria Street, Darlinghurst
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2010
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298353/suppl/GSM298353.CEL.gz
| | Sample_series_id | GSE11791
| | Sample_data_row_count | 54675
| | |
|
GSM298354 | GPL570 |
|
MCF7_EtOH_rep2
|
MCF-7, ICI 182780 pretreatment then treated with ethanol vehicle
|
MCF-7 human breast cancer cells
|
MCF-7 cells pretreated with ICI 182780 then treated with ethanol vehicle
|
| Sample_geo_accession | GSM298354
| | Sample_status | Public on Aug 21 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Aug 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Exponentially proliferating cells were growth arrested by pretreatment for 48 h with 10nM steroidal antiestrogen ICI 182780 and then treated with either 100nM E2 or 75 uM Zn (as ZnSO4). Vehicle controls for E2 or Zn were absolute ethanol and water respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from three independent experiments was isolated from cells using the RNeasy mini kit (Qiagen Pty Ltd, Clifton Hill, Vic Australia) following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fluorescent cRNA probes for Affymetrix oligonucleotide microarray analysis were generated using the One-cycle Target labelling and Control Reagent package (Millennium Science, Box Hill, Vic Australia) according to the manufacturer's instructions
| | Sample_hyb_protocol | Probes were hybridised at 45 C for 16 h and then washed and doubly stained using the GeneChip 400 fluidics station according to the manufacturer's instructions
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner (Affymetrix- Millennium Science, Surrey Hill, VIC, Australia)
| | Sample_data_processing | Signals obtained were analysed with Microarray Suite expression algorithm (version 5.0.1; Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Liz,,Musgrove
| | Sample_contact_email | e.musgrove@garvan.org.au
| | Sample_contact_phone | +61 2 9295 8328
| | Sample_contact_fax | +61 2 9295 8321
| | Sample_contact_department | Cancer Research Program
| | Sample_contact_institute | Garvan Institute of Medical Research
| | Sample_contact_address | 384 Victoria Street, Darlinghurst
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2010
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298354/suppl/GSM298354.CEL.gz
| | Sample_series_id | GSE11791
| | Sample_data_row_count | 54675
| | |
|
GSM298355 | GPL570 |
|
MCF7_EtOH_rep3
|
MCF-7, ICI 182780 pretreatment then treated with ethanol vehicle
|
MCF-7 human breast cancer cells
|
MCF-7 cells pretreated with ICI 182780 then treated with ethanol vehicle
|
| Sample_geo_accession | GSM298355
| | Sample_status | Public on Aug 21 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Aug 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Exponentially proliferating cells were growth arrested by pretreatment for 48 h with 10nM steroidal antiestrogen ICI 182780 and then treated with either 100nM E2 or 75 uM Zn (as ZnSO4). Vehicle controls for E2 or Zn were absolute ethanol and water respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from three independent experiments was isolated from cells using the RNeasy mini kit (Qiagen Pty Ltd, Clifton Hill, Vic Australia) following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fluorescent cRNA probes for Affymetrix oligonucleotide microarray analysis were generated using the One-cycle Target labelling and Control Reagent package (Millennium Science, Box Hill, Vic Australia) according to the manufacturer's instructions
| | Sample_hyb_protocol | Probes were hybridised at 45 C for 16 h and then washed and doubly stained using the GeneChip 400 fluidics station according to the manufacturer's instructions
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner (Affymetrix- Millennium Science, Surrey Hill, VIC, Australia)
| | Sample_data_processing | Signals obtained were analysed with Microarray Suite expression algorithm (version 5.0.1; Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Liz,,Musgrove
| | Sample_contact_email | e.musgrove@garvan.org.au
| | Sample_contact_phone | +61 2 9295 8328
| | Sample_contact_fax | +61 2 9295 8321
| | Sample_contact_department | Cancer Research Program
| | Sample_contact_institute | Garvan Institute of Medical Research
| | Sample_contact_address | 384 Victoria Street, Darlinghurst
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2010
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298355/suppl/GSM298355.CEL.gz
| | Sample_series_id | GSE11791
| | Sample_data_row_count | 54675
| | |
|
GSM298356 | GPL570 |
|
MCF7_E2_rep1
|
MCF-7, ICI 182780 pretreatment then treated with E2
|
MCF-7 human breast cancer cells
|
MCF-7 cells pretreated with ICI 182780 then treated with estrogen
|
| Sample_geo_accession | GSM298356
| | Sample_status | Public on Aug 21 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Aug 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Exponentially proliferating cells were growth arrested by pretreatment for 48 h with 10nM steroidal antiestrogen ICI 182780 and then treated with either 100nM E2 or 75 uM Zn (as ZnSO4). Vehicle controls for E2 or Zn were absolute ethanol and water respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from three independent experiments was isolated from cells using the RNeasy mini kit (Qiagen Pty Ltd, Clifton Hill, Vic Australia) following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fluorescent cRNA probes for Affymetrix oligonucleotide microarray analysis were generated using the One-cycle Target labelling and Control Reagent package (Millennium Science, Box Hill, Vic Australia) according to the manufacturer's instructions
| | Sample_hyb_protocol | Probes were hybridised at 45 C for 16 h and then washed and doubly stained using the GeneChip 400 fluidics station according to the manufacturer's instructions
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner (Affymetrix- Millennium Science, Surrey Hill, VIC, Australia)
| | Sample_data_processing | Signals obtained were analysed with Microarray Suite expression algorithm (version 5.0.1; Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Liz,,Musgrove
| | Sample_contact_email | e.musgrove@garvan.org.au
| | Sample_contact_phone | +61 2 9295 8328
| | Sample_contact_fax | +61 2 9295 8321
| | Sample_contact_department | Cancer Research Program
| | Sample_contact_institute | Garvan Institute of Medical Research
| | Sample_contact_address | 384 Victoria Street, Darlinghurst
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2010
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298356/suppl/GSM298356.CEL.gz
| | Sample_series_id | GSE11791
| | Sample_data_row_count | 54675
| | |
|
GSM298357 | GPL570 |
|
MCF7_E2_rep2
|
MCF-7, ICI 182780 pretreatment then treated with E2
|
MCF-7 human breast cancer cells
|
MCF-7 cells pretreated with ICI 182780 then treated with estrogen
|
| Sample_geo_accession | GSM298357
| | Sample_status | Public on Aug 21 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Aug 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Exponentially proliferating cells were growth arrested by pretreatment for 48 h with 10nM steroidal antiestrogen ICI 182780 and then treated with either 100nM E2 or 75 uM Zn (as ZnSO4). Vehicle controls for E2 or Zn were absolute ethanol and water respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from three independent experiments was isolated from cells using the RNeasy mini kit (Qiagen Pty Ltd, Clifton Hill, Vic Australia) following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fluorescent cRNA probes for Affymetrix oligonucleotide microarray analysis were generated using the One-cycle Target labelling and Control Reagent package (Millennium Science, Box Hill, Vic Australia) according to the manufacturer's instructions
| | Sample_hyb_protocol | Probes were hybridised at 45 C for 16 h and then washed and doubly stained using the GeneChip 400 fluidics station according to the manufacturer's instructions
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner (Affymetrix- Millennium Science, Surrey Hill, VIC, Australia)
| | Sample_data_processing | Signals obtained were analysed with Microarray Suite expression algorithm (version 5.0.1; Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Liz,,Musgrove
| | Sample_contact_email | e.musgrove@garvan.org.au
| | Sample_contact_phone | +61 2 9295 8328
| | Sample_contact_fax | +61 2 9295 8321
| | Sample_contact_department | Cancer Research Program
| | Sample_contact_institute | Garvan Institute of Medical Research
| | Sample_contact_address | 384 Victoria Street, Darlinghurst
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2010
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298357/suppl/GSM298357.CEL.gz
| | Sample_series_id | GSE11791
| | Sample_data_row_count | 54675
| | |
|
GSM298358 | GPL570 |
|
MCF7_E2_rep3
|
MCF-7, ICI 182780 pretreatment then treated with E2
|
MCF-7 human breast cancer cells
|
MCF-7 cells pretreated with ICI 182780 then treated with estrogen
|
| Sample_geo_accession | GSM298358
| | Sample_status | Public on Aug 21 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Aug 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Exponentially proliferating cells were growth arrested by pretreatment for 48 h with 10nM steroidal antiestrogen ICI 182780 and then treated with either 100nM E2 or 75 uM Zn (as ZnSO4). Vehicle controls for E2 or Zn were absolute ethanol and water respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from three independent experiments was isolated from cells using the RNeasy mini kit (Qiagen Pty Ltd, Clifton Hill, Vic Australia) following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fluorescent cRNA probes for Affymetrix oligonucleotide microarray analysis were generated using the One-cycle Target labelling and Control Reagent package (Millennium Science, Box Hill, Vic Australia) according to the manufacturer's instructions
| | Sample_hyb_protocol | Probes were hybridised at 45 C for 16 h and then washed and doubly stained using the GeneChip 400 fluidics station according to the manufacturer's instructions
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner (Affymetrix- Millennium Science, Surrey Hill, VIC, Australia)
| | Sample_data_processing | Signals obtained were analysed with Microarray Suite expression algorithm (version 5.0.1; Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Liz,,Musgrove
| | Sample_contact_email | e.musgrove@garvan.org.au
| | Sample_contact_phone | +61 2 9295 8328
| | Sample_contact_fax | +61 2 9295 8321
| | Sample_contact_department | Cancer Research Program
| | Sample_contact_institute | Garvan Institute of Medical Research
| | Sample_contact_address | 384 Victoria Street, Darlinghurst
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2010
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298358/suppl/GSM298358.CEL.gz
| | Sample_series_id | GSE11791
| | Sample_data_row_count | 54675
| | |
|
GSM298359 | GPL570 |
|
Vector_Zn_rep1
|
Clonal MCF-7 ∆MT, ICI 182780 pretreatment then treated with Zn
|
Clonal derivative of MCF-7 transfected with empty vector (p∆MT)
|
Clonal MCF-7 cells inducibly expressing c-Myc pretreated with ICI 182780 then treated with zinc
|
| Sample_geo_accession | GSM298359
| | Sample_status | Public on Aug 21 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Aug 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Exponentially proliferating cells were growth arrested by pretreatment for 48 h with 10nM steroidal antiestrogen ICI 182780 and then treated with either 100nM E2 or 75 uM Zn (as ZnSO4). Vehicle controls for E2 or Zn were absolute ethanol and water respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from three independent experiments was isolated from cells using the RNeasy mini kit (Qiagen Pty Ltd, Clifton Hill, Vic Australia) following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fluorescent cRNA probes for Affymetrix oligonucleotide microarray analysis were generated using the One-cycle Target labelling and Control Reagent package (Millennium Science, Box Hill, Vic Australia) according to the manufacturer's instructions
| | Sample_hyb_protocol | Probes were hybridised at 45 C for 16 h and then washed and doubly stained using the GeneChip 400 fluidics station according to the manufacturer's instructions
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner (Affymetrix- Millennium Science, Surrey Hill, VIC, Australia)
| | Sample_data_processing | Signals obtained were analysed with Microarray Suite expression algorithm (version 5.0.1; Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Liz,,Musgrove
| | Sample_contact_email | e.musgrove@garvan.org.au
| | Sample_contact_phone | +61 2 9295 8328
| | Sample_contact_fax | +61 2 9295 8321
| | Sample_contact_department | Cancer Research Program
| | Sample_contact_institute | Garvan Institute of Medical Research
| | Sample_contact_address | 384 Victoria Street, Darlinghurst
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2010
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298359/suppl/GSM298359.CEL.gz
| | Sample_series_id | GSE11791
| | Sample_data_row_count | 54675
| | |
|
GSM298360 | GPL570 |
|
Vector_Zn_rep2
|
Clonal MCF-7 ∆MT, ICI 182780 pretreatment then treated with Zn
|
Clonal derivative of MCF-7 transfected with empty vector (p∆MT)
|
Clonal MCF-7 cells (empty vector trasnsfected) pretreated with ICI 182780 then treated with zinc
|
| Sample_geo_accession | GSM298360
| | Sample_status | Public on Aug 21 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Aug 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Exponentially proliferating cells were growth arrested by pretreatment for 48 h with 10nM steroidal antiestrogen ICI 182780 and then treated with either 100nM E2 or 75 uM Zn (as ZnSO4). Vehicle controls for E2 or Zn were absolute ethanol and water respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from three independent experiments was isolated from cells using the RNeasy mini kit (Qiagen Pty Ltd, Clifton Hill, Vic Australia) following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fluorescent cRNA probes for Affymetrix oligonucleotide microarray analysis were generated using the One-cycle Target labelling and Control Reagent package (Millennium Science, Box Hill, Vic Australia) according to the manufacturer's instructions
| | Sample_hyb_protocol | Probes were hybridised at 45 C for 16 h and then washed and doubly stained using the GeneChip 400 fluidics station according to the manufacturer's instructions
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner (Affymetrix- Millennium Science, Surrey Hill, VIC, Australia)
| | Sample_data_processing | Signals obtained were analysed with Microarray Suite expression algorithm (version 5.0.1; Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Liz,,Musgrove
| | Sample_contact_email | e.musgrove@garvan.org.au
| | Sample_contact_phone | +61 2 9295 8328
| | Sample_contact_fax | +61 2 9295 8321
| | Sample_contact_department | Cancer Research Program
| | Sample_contact_institute | Garvan Institute of Medical Research
| | Sample_contact_address | 384 Victoria Street, Darlinghurst
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2010
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298360/suppl/GSM298360.CEL.gz
| | Sample_series_id | GSE11791
| | Sample_data_row_count | 54675
| | |
|
GSM298361 | GPL570 |
|
Vector_Zn_rep3
|
Clonal MCF-7 ∆MT, ICI 182780 pretreatment then treated with Zn
|
Clonal derivative of MCF-7 transfected with empty vector (p∆MT)
|
Clonal MCF-7 cells (empty vector trasnsfected) pretreated with ICI 182780 then treated with zinc
|
| Sample_geo_accession | GSM298361
| | Sample_status | Public on Aug 21 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Aug 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Exponentially proliferating cells were growth arrested by pretreatment for 48 h with 10nM steroidal antiestrogen ICI 182780 and then treated with either 100nM E2 or 75 uM Zn (as ZnSO4). Vehicle controls for E2 or Zn were absolute ethanol and water respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from three independent experiments was isolated from cells using the RNeasy mini kit (Qiagen Pty Ltd, Clifton Hill, Vic Australia) following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fluorescent cRNA probes for Affymetrix oligonucleotide microarray analysis were generated using the One-cycle Target labelling and Control Reagent package (Millennium Science, Box Hill, Vic Australia) according to the manufacturer's instructions
| | Sample_hyb_protocol | Probes were hybridised at 45 C for 16 h and then washed and doubly stained using the GeneChip 400 fluidics station according to the manufacturer's instructions
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner (Affymetrix- Millennium Science, Surrey Hill, VIC, Australia)
| | Sample_data_processing | Signals obtained were analysed with Microarray Suite expression algorithm (version 5.0.1; Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Liz,,Musgrove
| | Sample_contact_email | e.musgrove@garvan.org.au
| | Sample_contact_phone | +61 2 9295 8328
| | Sample_contact_fax | +61 2 9295 8321
| | Sample_contact_department | Cancer Research Program
| | Sample_contact_institute | Garvan Institute of Medical Research
| | Sample_contact_address | 384 Victoria Street, Darlinghurst
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2010
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298361/suppl/GSM298361.CEL.gz
| | Sample_series_id | GSE11791
| | Sample_data_row_count | 54675
| | |
|
GSM298362 | GPL570 |
|
Myc_Zn_rep1
|
Clonal MCF-7 ∆MT-Myc, ICI 182780 pretreatment then treated with Zn
|
Clonal derivative of MCF-7 transfected with inducible vector expressing c-Myc (p∆MT-Myc)
|
Clonal MCF-7 cells inducibly expressing c-Myc pretreated with ICI 182780 then treated with zinc
|
| Sample_geo_accession | GSM298362
| | Sample_status | Public on Aug 21 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Aug 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Exponentially proliferating cells were growth arrested by pretreatment for 48 h with 10nM steroidal antiestrogen ICI 182780 and then treated with either 100nM E2 or 75 uM Zn (as ZnSO4). Vehicle controls for E2 or Zn were absolute ethanol and water respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from three independent experiments was isolated from cells using the RNeasy mini kit (Qiagen Pty Ltd, Clifton Hill, Vic Australia) following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fluorescent cRNA probes for Affymetrix oligonucleotide microarray analysis were generated using the One-cycle Target labelling and Control Reagent package (Millennium Science, Box Hill, Vic Australia) according to the manufacturer's instructions
| | Sample_hyb_protocol | Probes were hybridised at 45 C for 16 h and then washed and doubly stained using the GeneChip 400 fluidics station according to the manufacturer's instructions
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner (Affymetrix- Millennium Science, Surrey Hill, VIC, Australia)
| | Sample_data_processing | Signals obtained were analysed with Microarray Suite expression algorithm (version 5.0.1; Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Liz,,Musgrove
| | Sample_contact_email | e.musgrove@garvan.org.au
| | Sample_contact_phone | +61 2 9295 8328
| | Sample_contact_fax | +61 2 9295 8321
| | Sample_contact_department | Cancer Research Program
| | Sample_contact_institute | Garvan Institute of Medical Research
| | Sample_contact_address | 384 Victoria Street, Darlinghurst
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2010
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298362/suppl/GSM298362.CEL.gz
| | Sample_series_id | GSE11791
| | Sample_data_row_count | 54675
| | |
|
GSM298363 | GPL570 |
|
Myc_Zn_rep2
|
Clonal MCF-7 ∆MT-Myc, ICI 182780 pretreatment then treated with Zn
|
Clonal derivative of MCF-7 transfected with inducible vector expressing c-Myc (p∆MT-Myc)
|
Clonal MCF-7 cells inducibly expressing c-Myc pretreated with ICI 182780 then treated with zinc
|
| Sample_geo_accession | GSM298363
| | Sample_status | Public on Aug 21 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Aug 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Exponentially proliferating cells were growth arrested by pretreatment for 48 h with 10nM steroidal antiestrogen ICI 182780 and then treated with either 100nM E2 or 75 uM Zn (as ZnSO4). Vehicle controls for E2 or Zn were absolute ethanol and water respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from three independent experiments was isolated from cells using the RNeasy mini kit (Qiagen Pty Ltd, Clifton Hill, Vic Australia) following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fluorescent cRNA probes for Affymetrix oligonucleotide microarray analysis were generated using the One-cycle Target labelling and Control Reagent package (Millennium Science, Box Hill, Vic Australia) according to the manufacturer's instructions
| | Sample_hyb_protocol | Probes were hybridised at 45 C for 16 h and then washed and doubly stained using the GeneChip 400 fluidics station according to the manufacturer's instructions
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner (Affymetrix- Millennium Science, Surrey Hill, VIC, Australia)
| | Sample_data_processing | Signals obtained were analysed with Microarray Suite expression algorithm (version 5.0.1; Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Liz,,Musgrove
| | Sample_contact_email | e.musgrove@garvan.org.au
| | Sample_contact_phone | +61 2 9295 8328
| | Sample_contact_fax | +61 2 9295 8321
| | Sample_contact_department | Cancer Research Program
| | Sample_contact_institute | Garvan Institute of Medical Research
| | Sample_contact_address | 384 Victoria Street, Darlinghurst
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2010
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298363/suppl/GSM298363.CEL.gz
| | Sample_series_id | GSE11791
| | Sample_data_row_count | 54675
| | |
|
GSM298364 | GPL570 |
|
Myc_Zn_rep3
|
Clonal MCF-7 ∆MT-Myc, ICI 182780 pretreatment then treated with Zn
|
Clonal derivative of MCF-7 transfected with inducible vector expressing c-Myc (p∆MT-Myc)
|
Clonal MCF-7 cells inducibly expressing c-Myc pretreated with ICI 182780 then treated with zinc
|
| Sample_geo_accession | GSM298364
| | Sample_status | Public on Aug 21 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Aug 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Exponentially proliferating cells were growth arrested by pretreatment for 48 h with 10nM steroidal antiestrogen ICI 182780 and then treated with either 100nM E2 or 75 uM Zn (as ZnSO4). Vehicle controls for E2 or Zn were absolute ethanol and water respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from three independent experiments was isolated from cells using the RNeasy mini kit (Qiagen Pty Ltd, Clifton Hill, Vic Australia) following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fluorescent cRNA probes for Affymetrix oligonucleotide microarray analysis were generated using the One-cycle Target labelling and Control Reagent package (Millennium Science, Box Hill, Vic Australia) according to the manufacturer's instructions
| | Sample_hyb_protocol | Probes were hybridised at 45 C for 16 h and then washed and doubly stained using the GeneChip 400 fluidics station according to the manufacturer's instructions
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner (Affymetrix- Millennium Science, Surrey Hill, VIC, Australia)
| | Sample_data_processing | Signals obtained were analysed with Microarray Suite expression algorithm (version 5.0.1; Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Liz,,Musgrove
| | Sample_contact_email | e.musgrove@garvan.org.au
| | Sample_contact_phone | +61 2 9295 8328
| | Sample_contact_fax | +61 2 9295 8321
| | Sample_contact_department | Cancer Research Program
| | Sample_contact_institute | Garvan Institute of Medical Research
| | Sample_contact_address | 384 Victoria Street, Darlinghurst
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2010
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298364/suppl/GSM298364.CEL.gz
| | Sample_series_id | GSE11791
| | Sample_data_row_count | 54675
| | |
|
GSM298365 | GPL570 |
|
Zip_Zn_rep1
|
Clonal MCF-7 ∆MT-Zip, ICI 182780 pretreatment then treated with Zn
|
Clonal derivative of MCF-7 transfected with inducible vector expressing c-Zip (p∆MT-Zip)
|
Clonal MCF-7 cells inducibly expressing c-Zip pretreated with ICI 182780 then treated with zinc
|
| Sample_geo_accession | GSM298365
| | Sample_status | Public on Aug 21 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Aug 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Exponentially proliferating cells were growth arrested by pretreatment for 48 h with 10nM steroidal antiestrogen ICI 182780 and then treated with either 100nM E2 or 75 uM Zn (as ZnSO4). Vehicle controls for E2 or Zn were absolute ethanol and water respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from three independent experiments was isolated from cells using the RNeasy mini kit (Qiagen Pty Ltd, Clifton Hill, Vic Australia) following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fluorescent cRNA probes for Affymetrix oligonucleotide microarray analysis were generated using the One-cycle Target labelling and Control Reagent package (Millennium Science, Box Hill, Vic Australia) according to the manufacturer's instructions
| | Sample_hyb_protocol | Probes were hybridised at 45 C for 16 h and then washed and doubly stained using the GeneChip 400 fluidics station according to the manufacturer's instructions
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner (Affymetrix- Millennium Science, Surrey Hill, VIC, Australia)
| | Sample_data_processing | Signals obtained were analysed with Microarray Suite expression algorithm (version 5.0.1; Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Liz,,Musgrove
| | Sample_contact_email | e.musgrove@garvan.org.au
| | Sample_contact_phone | +61 2 9295 8328
| | Sample_contact_fax | +61 2 9295 8321
| | Sample_contact_department | Cancer Research Program
| | Sample_contact_institute | Garvan Institute of Medical Research
| | Sample_contact_address | 384 Victoria Street, Darlinghurst
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2010
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298365/suppl/GSM298365.CEL.gz
| | Sample_series_id | GSE11791
| | Sample_data_row_count | 54675
| | |
|
GSM298366 | GPL570 |
|
Zip_Zn_rep2
|
Clonal MCF-7 ∆MT-Zip, ICI 182780 pretreatment then treated with Zn
|
Clonal derivative of MCF-7 transfected with inducible vector expressing c-Zip (p∆MT-Zip)
|
Clonal MCF-7 cells inducibly expressing c-Zip pretreated with ICI 182780 then treated with zinc
|
| Sample_geo_accession | GSM298366
| | Sample_status | Public on Aug 21 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Aug 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Exponentially proliferating cells were growth arrested by pretreatment for 48 h with 10nM steroidal antiestrogen ICI 182780 and then treated with either 100nM E2 or 75 uM Zn (as ZnSO4). Vehicle controls for E2 or Zn were absolute ethanol and water respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from three independent experiments was isolated from cells using the RNeasy mini kit (Qiagen Pty Ltd, Clifton Hill, Vic Australia) following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fluorescent cRNA probes for Affymetrix oligonucleotide microarray analysis were generated using the One-cycle Target labelling and Control Reagent package (Millennium Science, Box Hill, Vic Australia) according to the manufacturer's instructions
| | Sample_hyb_protocol | Probes were hybridised at 45 C for 16 h and then washed and doubly stained using the GeneChip 400 fluidics station according to the manufacturer's instructions
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner (Affymetrix- Millennium Science, Surrey Hill, VIC, Australia)
| | Sample_data_processing | Signals obtained were analysed with Microarray Suite expression algorithm (version 5.0.1; Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Liz,,Musgrove
| | Sample_contact_email | e.musgrove@garvan.org.au
| | Sample_contact_phone | +61 2 9295 8328
| | Sample_contact_fax | +61 2 9295 8321
| | Sample_contact_department | Cancer Research Program
| | Sample_contact_institute | Garvan Institute of Medical Research
| | Sample_contact_address | 384 Victoria Street, Darlinghurst
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2010
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298366/suppl/GSM298366.CEL.gz
| | Sample_series_id | GSE11791
| | Sample_data_row_count | 54675
| | |
|
GSM298367 | GPL570 |
|
Zip_Zn_rep3
|
Clonal MCF-7 ∆MT-Zip, ICI 182780 pretreatment then treated with Zn
|
Clonal derivative of MCF-7 transfected with inducible vector expressing c-ZIp (p∆MT-Zip)
|
Clonal MCF-7 cells inducibly expressing c-Zip pretreated with ICI 182780 then treated with zinc
|
| Sample_geo_accession | GSM298367
| | Sample_status | Public on Aug 21 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Aug 19 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Exponentially proliferating cells were growth arrested by pretreatment for 48 h with 10nM steroidal antiestrogen ICI 182780 and then treated with either 100nM E2 or 75 uM Zn (as ZnSO4). Vehicle controls for E2 or Zn were absolute ethanol and water respectively.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from three independent experiments was isolated from cells using the RNeasy mini kit (Qiagen Pty Ltd, Clifton Hill, Vic Australia) following the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Fluorescent cRNA probes for Affymetrix oligonucleotide microarray analysis were generated using the One-cycle Target labelling and Control Reagent package (Millennium Science, Box Hill, Vic Australia) according to the manufacturer's instructions
| | Sample_hyb_protocol | Probes were hybridised at 45 C for 16 h and then washed and doubly stained using the GeneChip 400 fluidics station according to the manufacturer's instructions
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner (Affymetrix- Millennium Science, Surrey Hill, VIC, Australia)
| | Sample_data_processing | Signals obtained were analysed with Microarray Suite expression algorithm (version 5.0.1; Affymetrix)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Liz,,Musgrove
| | Sample_contact_email | e.musgrove@garvan.org.au
| | Sample_contact_phone | +61 2 9295 8328
| | Sample_contact_fax | +61 2 9295 8321
| | Sample_contact_department | Cancer Research Program
| | Sample_contact_institute | Garvan Institute of Medical Research
| | Sample_contact_address | 384 Victoria Street, Darlinghurst
| | Sample_contact_city | Sydney
| | Sample_contact_state | NSW
| | Sample_contact_zip/postal_code | 2010
| | Sample_contact_country | Australia
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298367/suppl/GSM298367.CEL.gz
| | Sample_series_id | GSE11791
| | Sample_data_row_count | 54675
| | |
|
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(2 groups will be compared at a time) |
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