Search results for the GEO ID: GSE11804  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM298558 | GPL1261 |
|
AICAR 1 (set 2)
|
Rodent quadricep muscles
|
10 wks old male C57Bl/6J mice
|
Treated with AICAR for 6 days
|
| Sample_geo_accession | GSM298558
| | Sample_status | Public on Jun 18 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Jun 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were treated with vehicle, AICAR (250mg/kg/day), GW1516 (5mg/kg/day) or the combination for 6 days. At the end of treatment, mice in each group were euthanized, quadricep muscle were immediately disceted and flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | N/A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from the frozen samples was extracted using Trizol method. For each sample, total RNA from 3 mice within the group was pooled.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Double straned DNA was prepared from 10ug of total RNA, which in turn was biotinylated using standard protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Expression Array 430A and Mouse Genome 430A 2.0 Array.
| | Sample_scan_protocol | Gene chips were scanned using Affymetrix High Resolution Genechip scanner 3000
| | Sample_data_processing | Data was processed using Affymetrix Microarray Suite 5.0 software. The trimmed mean signal of all probe sets was adjusted to a user specific target signal value for each array for global scaling.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ruth,T,Yu
| | Sample_contact_laboratory | Ronald Evans
| | Sample_contact_department | Gene Expression Lab
| | Sample_contact_institute | Salk Institute
| | Sample_contact_address | 10010 N Torrey Pines Rd
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298558/suppl/GSM298558.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298558/suppl/GSM298558.CHP.gz
| | Sample_series_id | GSE11804
| | Sample_series_id | GSE11805
| | Sample_data_row_count | 45101
| | |
|
GSM298559 | GPL1261 |
|
AICAR 2 (set 2)
|
Rodent quadricep muscles
|
10 wks old male C57Bl/6J mice
|
Treated with AICAR for 6 days
|
| Sample_geo_accession | GSM298559
| | Sample_status | Public on Jun 18 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Jun 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were treated with vehicle, AICAR (250mg/kg/day), GW1516 (5mg/kg/day) or the combination for 6 days. At the end of treatment, mice in each group were euthanized, quadricep muscle were immediately disceted and flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | N/A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from the frozen samples was extracted using Trizol method. For each sample, total RNA from 3 mice within the group was pooled.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Double straned DNA was prepared from 10ug of total RNA, which in turn was biotinylated using standard protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Expression Array 430A and Mouse Genome 430A 2.0 Array.
| | Sample_scan_protocol | Gene chips were scanned using Affymetrix High Resolution Genechip scanner 3000
| | Sample_data_processing | Data was processed using Affymetrix Microarray Suite 5.0 software. The trimmed mean signal of all probe sets was adjusted to a user specific target signal value for each array for global scaling.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ruth,T,Yu
| | Sample_contact_laboratory | Ronald Evans
| | Sample_contact_department | Gene Expression Lab
| | Sample_contact_institute | Salk Institute
| | Sample_contact_address | 10010 N Torrey Pines Rd
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298559/suppl/GSM298559.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298559/suppl/GSM298559.CHP.gz
| | Sample_series_id | GSE11804
| | Sample_series_id | GSE11805
| | Sample_data_row_count | 45101
| | |
|
GSM298560 | GPL1261 |
|
AICAR 3 (set 2)
|
Rodent quadricep muscles
|
10 wks old male C57Bl/6J mice
|
Treated with AICAR for 6 days
|
| Sample_geo_accession | GSM298560
| | Sample_status | Public on Jun 18 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Jun 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were treated with vehicle, AICAR (250mg/kg/day), GW1516 (5mg/kg/day) or the combination for 6 days. At the end of treatment, mice in each group were euthanized, quadricep muscle were immediately disceted and flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | N/A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from the frozen samples was extracted using Trizol method. For each sample, total RNA from 3 mice within the group was pooled.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Double straned DNA was prepared from 10ug of total RNA, which in turn was biotinylated using standard protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Expression Array 430A and Mouse Genome 430A 2.0 Array.
| | Sample_scan_protocol | Gene chips were scanned using Affymetrix High Resolution Genechip scanner 3000
| | Sample_data_processing | Data was processed using Affymetrix Microarray Suite 5.0 software. The trimmed mean signal of all probe sets was adjusted to a user specific target signal value for each array for global scaling.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ruth,T,Yu
| | Sample_contact_laboratory | Ronald Evans
| | Sample_contact_department | Gene Expression Lab
| | Sample_contact_institute | Salk Institute
| | Sample_contact_address | 10010 N Torrey Pines Rd
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298560/suppl/GSM298560.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298560/suppl/GSM298560.CHP.gz
| | Sample_series_id | GSE11804
| | Sample_series_id | GSE11805
| | Sample_data_row_count | 45101
| | |
|
GSM298561 | GPL1261 |
|
GW 1 (set 2)
|
Rodent quadricep muscles
|
10 wks old male C57Bl/6J mice
|
Treated with GW1516 for 6 days
|
| Sample_geo_accession | GSM298561
| | Sample_status | Public on Jun 18 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Jun 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were treated with vehicle, AICAR (250mg/kg/day), GW1516 (5mg/kg/day) or the combination for 6 days. At the end of treatment, mice in each group were euthanized, quadricep muscle were immediately disceted and flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | N/A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from the frozen samples was extracted using Trizol method. For each sample, total RNA from 3 mice within the group was pooled.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Double straned DNA was prepared from 10ug of total RNA, which in turn was biotinylated using standard protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Expression Array 430A and Mouse Genome 430A 2.0 Array.
| | Sample_scan_protocol | Gene chips were scanned using Affymetrix High Resolution Genechip scanner 3000
| | Sample_data_processing | Data was processed using Affymetrix Microarray Suite 5.0 software. The trimmed mean signal of all probe sets was adjusted to a user specific target signal value for each array for global scaling.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ruth,T,Yu
| | Sample_contact_laboratory | Ronald Evans
| | Sample_contact_department | Gene Expression Lab
| | Sample_contact_institute | Salk Institute
| | Sample_contact_address | 10010 N Torrey Pines Rd
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298561/suppl/GSM298561.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298561/suppl/GSM298561.CHP.gz
| | Sample_series_id | GSE11804
| | Sample_series_id | GSE11805
| | Sample_data_row_count | 45101
| | |
|
GSM298562 | GPL1261 |
|
GW 2 (set 2)
|
Rodent quadricep muscles
|
10 wks old male C57Bl/6J mice
|
Treated with GW1516 for 6 days
|
| Sample_geo_accession | GSM298562
| | Sample_status | Public on Jun 18 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Jun 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were treated with vehicle, AICAR (250mg/kg/day), GW1516 (5mg/kg/day) or the combination for 6 days. At the end of treatment, mice in each group were euthanized, quadricep muscle were immediately disceted and flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | N/A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from the frozen samples was extracted using Trizol method. For each sample, total RNA from 3 mice within the group was pooled.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Double straned DNA was prepared from 10ug of total RNA, which in turn was biotinylated using standard protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Expression Array 430A and Mouse Genome 430A 2.0 Array.
| | Sample_scan_protocol | Gene chips were scanned using Affymetrix High Resolution Genechip scanner 3000
| | Sample_data_processing | Data was processed using Affymetrix Microarray Suite 5.0 software. The trimmed mean signal of all probe sets was adjusted to a user specific target signal value for each array for global scaling.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ruth,T,Yu
| | Sample_contact_laboratory | Ronald Evans
| | Sample_contact_department | Gene Expression Lab
| | Sample_contact_institute | Salk Institute
| | Sample_contact_address | 10010 N Torrey Pines Rd
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298562/suppl/GSM298562.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298562/suppl/GSM298562.CHP.gz
| | Sample_series_id | GSE11804
| | Sample_series_id | GSE11805
| | Sample_data_row_count | 45101
| | |
|
GSM298563 | GPL1261 |
|
GW 3 (set 2)
|
Rodent quadricep muscles
|
10 wks old male C57Bl/6J mice
|
Treated with GW1516 for 6 days
|
| Sample_geo_accession | GSM298563
| | Sample_status | Public on Jun 18 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Jun 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were treated with vehicle, AICAR (250mg/kg/day), GW1516 (5mg/kg/day) or the combination for 6 days. At the end of treatment, mice in each group were euthanized, quadricep muscle were immediately disceted and flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | N/A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from the frozen samples was extracted using Trizol method. For each sample, total RNA from 3 mice within the group was pooled.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Double straned DNA was prepared from 10ug of total RNA, which in turn was biotinylated using standard protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Expression Array 430A and Mouse Genome 430A 2.0 Array.
| | Sample_scan_protocol | Gene chips were scanned using Affymetrix High Resolution Genechip scanner 3000
| | Sample_data_processing | Data was processed using Affymetrix Microarray Suite 5.0 software. The trimmed mean signal of all probe sets was adjusted to a user specific target signal value for each array for global scaling.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ruth,T,Yu
| | Sample_contact_laboratory | Ronald Evans
| | Sample_contact_department | Gene Expression Lab
| | Sample_contact_institute | Salk Institute
| | Sample_contact_address | 10010 N Torrey Pines Rd
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298563/suppl/GSM298563.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298563/suppl/GSM298563.CHP.gz
| | Sample_series_id | GSE11804
| | Sample_series_id | GSE11805
| | Sample_data_row_count | 45101
| | |
|
GSM298564 | GPL1261 |
|
GW + AICAR 1 (set 2)
|
Rodent quadricep muscles
|
10 wks old male C57Bl/6J mice
|
Treated with AICAR and GW for 6 days
|
| Sample_geo_accession | GSM298564
| | Sample_status | Public on Jun 18 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Jun 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were treated with vehicle, AICAR (250mg/kg/day), GW1516 (5mg/kg/day) or the combination for 6 days. At the end of treatment, mice in each group were euthanized, quadricep muscle were immediately disceted and flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | N/A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from the frozen samples was extracted using Trizol method. For each sample, total RNA from 3 mice within the group was pooled.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Double straned DNA was prepared from 10ug of total RNA, which in turn was biotinylated using standard protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Expression Array 430A and Mouse Genome 430A 2.0 Array.
| | Sample_scan_protocol | Gene chips were scanned using Affymetrix High Resolution Genechip scanner 3000
| | Sample_data_processing | Data was processed using Affymetrix Microarray Suite 5.0 software. The trimmed mean signal of all probe sets was adjusted to a user specific target signal value for each array for global scaling.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ruth,T,Yu
| | Sample_contact_laboratory | Ronald Evans
| | Sample_contact_department | Gene Expression Lab
| | Sample_contact_institute | Salk Institute
| | Sample_contact_address | 10010 N Torrey Pines Rd
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298564/suppl/GSM298564.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298564/suppl/GSM298564.CHP.gz
| | Sample_series_id | GSE11804
| | Sample_series_id | GSE11805
| | Sample_data_row_count | 45101
| | |
|
GSM298565 | GPL1261 |
|
GW + AICAR 2 (set 2)
|
Rodent quadricep muscles
|
10 wks old male C57Bl/6J mice
|
Treated with AICAR and GW for 6 days
|
| Sample_geo_accession | GSM298565
| | Sample_status | Public on Jun 18 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Jun 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were treated with vehicle, AICAR (250mg/kg/day), GW1516 (5mg/kg/day) or the combination for 6 days. At the end of treatment, mice in each group were euthanized, quadricep muscle were immediately disceted and flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | N/A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from the frozen samples was extracted using Trizol method. For each sample, total RNA from 3 mice within the group was pooled.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Double straned DNA was prepared from 10ug of total RNA, which in turn was biotinylated using standard protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Expression Array 430A and Mouse Genome 430A 2.0 Array.
| | Sample_scan_protocol | Gene chips were scanned using Affymetrix High Resolution Genechip scanner 3000
| | Sample_data_processing | Data was processed using Affymetrix Microarray Suite 5.0 software. The trimmed mean signal of all probe sets was adjusted to a user specific target signal value for each array for global scaling.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ruth,T,Yu
| | Sample_contact_laboratory | Ronald Evans
| | Sample_contact_department | Gene Expression Lab
| | Sample_contact_institute | Salk Institute
| | Sample_contact_address | 10010 N Torrey Pines Rd
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298565/suppl/GSM298565.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298565/suppl/GSM298565.CHP.gz
| | Sample_series_id | GSE11804
| | Sample_series_id | GSE11805
| | Sample_data_row_count | 45101
| | |
|
GSM298566 | GPL1261 |
|
GW + AICAR 3 (set 2)
|
Rodent quadricep muscles
|
10 wks old male C57Bl/6J mice
|
Treated with AICAR and GW for 6 days
|
| Sample_geo_accession | GSM298566
| | Sample_status | Public on Jun 18 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Jun 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were treated with vehicle, AICAR (250mg/kg/day), GW1516 (5mg/kg/day) or the combination for 6 days. At the end of treatment, mice in each group were euthanized, quadricep muscle were immediately disceted and flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | N/A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from the frozen samples was extracted using Trizol method. For each sample, total RNA from 3 mice within the group was pooled.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Double straned DNA was prepared from 10ug of total RNA, which in turn was biotinylated using standard protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Expression Array 430A and Mouse Genome 430A 2.0 Array.
| | Sample_scan_protocol | Gene chips were scanned using Affymetrix High Resolution Genechip scanner 3000
| | Sample_data_processing | Data was processed using Affymetrix Microarray Suite 5.0 software. The trimmed mean signal of all probe sets was adjusted to a user specific target signal value for each array for global scaling.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ruth,T,Yu
| | Sample_contact_laboratory | Ronald Evans
| | Sample_contact_department | Gene Expression Lab
| | Sample_contact_institute | Salk Institute
| | Sample_contact_address | 10010 N Torrey Pines Rd
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298566/suppl/GSM298566.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298566/suppl/GSM298566.CHP.gz
| | Sample_series_id | GSE11804
| | Sample_series_id | GSE11805
| | Sample_data_row_count | 45101
| | |
|
GSM298567 | GPL1261 |
|
Vehicle 1 (set 2)
|
Rodent quadricep muscles
|
10 wks old male C57Bl/6J mice
|
Treated with vehicle for 6 days
|
| Sample_geo_accession | GSM298567
| | Sample_status | Public on Jun 18 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Jun 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were treated with vehicle, AICAR (250mg/kg/day), GW1516 (5mg/kg/day) or the combination for 6 days. At the end of treatment, mice in each group were euthanized, quadricep muscle were immediately disceted and flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | N/A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from the frozen samples was extracted using Trizol method. For each sample, total RNA from 3 mice within the group was pooled.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Double straned DNA was prepared from 10ug of total RNA, which in turn was biotinylated using standard protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Expression Array 430A and Mouse Genome 430A 2.0 Array.
| | Sample_scan_protocol | Gene chips were scanned using Affymetrix High Resolution Genechip scanner 3000
| | Sample_data_processing | Data was processed using Affymetrix Microarray Suite 5.0 software. The trimmed mean signal of all probe sets was adjusted to a user specific target signal value for each array for global scaling.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ruth,T,Yu
| | Sample_contact_laboratory | Ronald Evans
| | Sample_contact_department | Gene Expression Lab
| | Sample_contact_institute | Salk Institute
| | Sample_contact_address | 10010 N Torrey Pines Rd
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298567/suppl/GSM298567.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298567/suppl/GSM298567.CHP.gz
| | Sample_series_id | GSE11804
| | Sample_series_id | GSE11805
| | Sample_data_row_count | 45101
| | |
|
GSM298568 | GPL1261 |
|
Vehicle 2 (set 2)
|
Rodent quadricep muscles
|
10 wks old male C57Bl/6J mice
|
Treated with vehicle for 6 days
|
| Sample_geo_accession | GSM298568
| | Sample_status | Public on Jun 18 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Jun 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were treated with vehicle, AICAR (250mg/kg/day), GW1516 (5mg/kg/day) or the combination for 6 days. At the end of treatment, mice in each group were euthanized, quadricep muscle were immediately disceted and flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | N/A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from the frozen samples was extracted using Trizol method. For each sample, total RNA from 3 mice within the group was pooled.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Double straned DNA was prepared from 10ug of total RNA, which in turn was biotinylated using standard protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Expression Array 430A and Mouse Genome 430A 2.0 Array.
| | Sample_scan_protocol | Gene chips were scanned using Affymetrix High Resolution Genechip scanner 3000
| | Sample_data_processing | Data was processed using Affymetrix Microarray Suite 5.0 software. The trimmed mean signal of all probe sets was adjusted to a user specific target signal value for each array for global scaling.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ruth,T,Yu
| | Sample_contact_laboratory | Ronald Evans
| | Sample_contact_department | Gene Expression Lab
| | Sample_contact_institute | Salk Institute
| | Sample_contact_address | 10010 N Torrey Pines Rd
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298568/suppl/GSM298568.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298568/suppl/GSM298568.CHP.gz
| | Sample_series_id | GSE11804
| | Sample_series_id | GSE11805
| | Sample_data_row_count | 45101
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GSM298569 | GPL1261 |
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Vehicle 3 (set 2)
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Rodent quadricep muscles
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10 wks old male C57Bl/6J mice
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Treated with vehicle for 6 days
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| Sample_geo_accession | GSM298569
| | Sample_status | Public on Jun 18 2008
| | Sample_submission_date | Jun 16 2008
| | Sample_last_update_date | Jun 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were treated with vehicle, AICAR (250mg/kg/day), GW1516 (5mg/kg/day) or the combination for 6 days. At the end of treatment, mice in each group were euthanized, quadricep muscle were immediately disceted and flash frozen in liquid nitrogen.
| | Sample_growth_protocol_ch1 | N/A
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from the frozen samples was extracted using Trizol method. For each sample, total RNA from 3 mice within the group was pooled.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Double straned DNA was prepared from 10ug of total RNA, which in turn was biotinylated using standard protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Expression Array 430A and Mouse Genome 430A 2.0 Array.
| | Sample_scan_protocol | Gene chips were scanned using Affymetrix High Resolution Genechip scanner 3000
| | Sample_data_processing | Data was processed using Affymetrix Microarray Suite 5.0 software. The trimmed mean signal of all probe sets was adjusted to a user specific target signal value for each array for global scaling.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Ruth,T,Yu
| | Sample_contact_laboratory | Ronald Evans
| | Sample_contact_department | Gene Expression Lab
| | Sample_contact_institute | Salk Institute
| | Sample_contact_address | 10010 N Torrey Pines Rd
| | Sample_contact_city | La Jolla
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92037
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298569/suppl/GSM298569.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298569/suppl/GSM298569.CHP.gz
| | Sample_series_id | GSE11804
| | Sample_series_id | GSE11805
| | Sample_data_row_count | 45101
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