Search results for the GEO ID: GSE11851  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM298719 | GPL1355 |
|
Week 6 vehicle control rat, heart right ventricle apex, biological rep1
|
Gene expression data from right ventricle apex RNA after 6 weeks of vehicle treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 vehicle control rat, heart right ventricle apex, biological rep1
|
| Sample_geo_accession | GSM298719
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of 0.01% Tween-20 as "vehicle" control treatment
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "A" for apex.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298719/suppl/GSM298719.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298719/suppl/GSM298719.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
|
GSM298720 | GPL1355 |
|
Week 6 vehicle control rat, heart right ventricle apex, biological rep2.
|
Gene expression data from right ventricle apex RNA after 6 weeks of vehicle treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 vehicle control rat, heart right ventricle apex, biological rep2.
|
| Sample_geo_accession | GSM298720
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of 0.01% Tween-20 as "vehicle" control treatment
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "A" for apex.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298720/suppl/GSM298720.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298720/suppl/GSM298720.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
|
GSM298721 | GPL1355 |
|
Week 6 vehicle control rat, heart right ventricle apex, biological rep3.
|
Gene expression data from right ventricle apex RNA after 6 weeks of vehicle treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 vehicle control rat, heart right ventricle apex, biological rep3.
|
| Sample_geo_accession | GSM298721
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of 0.01% Tween-20 as "vehicle" control treatment
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "A" for apex.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298721/suppl/GSM298721.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298721/suppl/GSM298721.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
|
GSM298722 | GPL1355 |
|
Week 6 vehicle control rat, heart right ventricle apex, biological rep4.
|
Gene expression data from right ventricle apex RNA after 6 weeks of vehicle treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 vehicle control rat, heart right ventricle apex, biological rep4.
|
| Sample_geo_accession | GSM298722
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of 0.01% Tween-20 as "vehicle" control treatment
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "A" for apex.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298722/suppl/GSM298722.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298722/suppl/GSM298722.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
|
GSM298723 | GPL1355 |
|
Week 6 vehicle control rat, heart right ventricle apex, biological rep5
|
Gene expression data from right ventricle apex RNA after 6 weeks of vehicle treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 vehicle control rat, heart right ventricle apex, biological rep5
|
| Sample_geo_accession | GSM298723
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of 0.01% Tween-20 as "vehicle" control treatment
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "A" for apex.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298723/suppl/GSM298723.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298723/suppl/GSM298723.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
|
GSM298724 | GPL1355 |
|
Week 6 vehicle control rat, heart right ventricle outflow, biological rep1
|
Gene expression data from right ventricle outflow RNA after 6 weeks of vehicle treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 vehicle control rat, heart right ventricle outflow, biological rep1
|
| Sample_geo_accession | GSM298724
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of 0.01% Tween-20 as "vehicle" control treatment
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "O" for outflow tract.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298724/suppl/GSM298724.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298724/suppl/GSM298724.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
|
GSM298725 | GPL1355 |
|
Week 6 vehicle control rat, heart right ventricle outflow, biological rep2
|
Gene expression data from right ventricle outflow RNA after 6 weeks of vehicle treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 vehicle control rat, heart right ventricle outflow, biological rep2
|
| Sample_geo_accession | GSM298725
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of 0.01% Tween-20 as "vehicle" control treatment
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "O" for outflow tract.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298725/suppl/GSM298725.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298725/suppl/GSM298725.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
|
GSM298726 | GPL1355 |
|
Week 6 vehicle control rat, heart right ventricle outflow, biological rep3
|
Gene expression data from right ventricle outflow RNA after 6 weeks of vehicle treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 vehicle control rat, heart right ventricle outflow, biological rep3
|
| Sample_geo_accession | GSM298726
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of 0.01% Tween-20 as "vehicle" control treatment
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "O" for outflow tract.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298726/suppl/GSM298726.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298726/suppl/GSM298726.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
|
GSM298727 | GPL1355 |
|
Week 6 vehicle control rat, heart right ventricle outflow, biological rep4
|
Gene expression data from right ventricle outflow RNA after 6 weeks of vehicle treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 vehicle control rat, heart right ventricle outflow, biological rep4
|
| Sample_geo_accession | GSM298727
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of 0.01% Tween-20 as "vehicle" control treatment
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "O" for outflow tract.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298727/suppl/GSM298727.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298727/suppl/GSM298727.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
|
GSM298728 | GPL1355 |
|
Week 6 vehicle control rat, heart right ventricle outflow, biological rep5
|
Gene expression data from right ventricle outflow RNA after 6 weeks of vehicle treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 vehicle control rat, heart right ventricle outflow, biological rep5
|
| Sample_geo_accession | GSM298728
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 0.15 ml/100 grams weight of 0.01% Tween-20 as "vehicle" control treatment
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "O" for outflow tract.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298728/suppl/GSM298728.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298728/suppl/GSM298728.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
|
GSM298729 | GPL1355 |
|
Week 6 PE2.0 rat, heart right ventricle apex, biological rep1
|
Gene expression data from right ventricle apex RNA after 6 weeks of microsphere treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 PE2.0 rat, heart right ventricle apex, biological rep1
|
| Sample_geo_accession | GSM298729
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 2 million (25 micron) polystyrene microspheres/100 grams weight at a concentration of 13 million microspheres/ml in 0.01% tween-20.
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "A" for apex.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298729/suppl/GSM298729.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298729/suppl/GSM298729.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
|
GSM298730 | GPL1355 |
|
Week 6 PE2.0 rat, heart right ventricle apex, biological rep2
|
Gene expression data from right ventricle apex RNA after 6 weeks of microsphere treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 PE2.0 rat, heart right ventricle apex, biological rep2
|
| Sample_geo_accession | GSM298730
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 2 million (25 micron) polystyrene microspheres/100 grams weight at a concentration of 13 million microspheres/ml in 0.01% tween-20.
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "A" for apex.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298730/suppl/GSM298730.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298730/suppl/GSM298730.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
|
GSM298731 | GPL1355 |
|
Week 6 PE2.0 rat, heart right ventricle apex, biological rep3
|
Gene expression data from right ventricle apex RNA after 6 weeks of microsphere treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 PE2.0 rat, heart right ventricle apex, biological rep3
|
| Sample_geo_accession | GSM298731
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 2 million (25 micron) polystyrene microspheres/100 grams weight at a concentration of 13 million microspheres/ml in 0.01% tween-20.
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "A" for apex.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298731/suppl/GSM298731.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298731/suppl/GSM298731.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
|
GSM298732 | GPL1355 |
|
Week 6 PE2.0 rat, heart right ventricle apex, biological rep4
|
Gene expression data from right ventricle apex RNA after 6 weeks of microsphere treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 PE2.0 rat, heart right ventricle apex, biological rep4
|
| Sample_geo_accession | GSM298732
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 2 million (25 micron) polystyrene microspheres/100 grams weight at a concentration of 13 million microspheres/ml in 0.01% tween-20.
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "A" for apex.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298732/suppl/GSM298732.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298732/suppl/GSM298732.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
|
GSM298733 | GPL1355 |
|
Week 6 PE2.0 rat, heart right ventricle apex, biological rep5
|
Gene expression data from right ventricle apex RNA after 6 weeks of microsphere treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 PE2.0 rat, heart right ventricle apex, biological rep5
|
| Sample_geo_accession | GSM298733
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 2 million (25 micron) polystyrene microspheres/100 grams weight at a concentration of 13 million microspheres/ml in 0.01% tween-20.
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "A" for apex.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298733/suppl/GSM298733.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298733/suppl/GSM298733.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
|
GSM298734 | GPL1355 |
|
Week 6 PE2.0 rat, heart right ventricle outflow, biological rep1
|
Gene expression data from right ventricle outflow RNA after 6 weeks of microsphere treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 PE2.0 rat, heart right ventricle outflow, biological rep1
|
| Sample_geo_accession | GSM298734
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 2 million (25 micron) polystyrene microspheres/100 grams weight at a concentration of 13 million microspheres/ml in 0.01% tween-20.
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "O" for outflow tract.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298734/suppl/GSM298734.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298734/suppl/GSM298734.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
|
GSM298735 | GPL1355 |
|
Week 6 PE2.0 rat, heart right ventricle outflow, biological rep2
|
Gene expression data from right ventricle outflow RNA after 6 weeks of microsphere treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 PE2.0 rat, heart right ventricle outflow, biological rep2
|
| Sample_geo_accession | GSM298735
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 2 million (25 micron) polystyrene microspheres/100 grams weight at a concentration of 13 million microspheres/ml in 0.01% tween-20.
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "O" for outflow tract.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298735/suppl/GSM298735.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298735/suppl/GSM298735.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
|
GSM298736 | GPL1355 |
|
Week 6 PE2.0 rat, heart right ventricle outflow, biological rep3
|
Gene expression data from right ventricle outflow RNA after 6 weeks of microsphere treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 PE2.0 rat, heart right ventricle outflow, biological rep3
|
| Sample_geo_accession | GSM298736
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 2 million (25 micron) polystyrene microspheres/100 grams weight at a concentration of 13 million microspheres/ml in 0.01% tween-20.
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "O" for outflow tract.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298736/suppl/GSM298736.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298736/suppl/GSM298736.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
|
GSM298737 | GPL1355 |
|
Week 6 PE2.0 rat, heart right ventricle outflow, biological rep4
|
Gene expression data from right ventricle outflow RNA after 6 weeks of microsphere treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 PE2.0 rat, heart right ventricle outflow, biological rep4
|
| Sample_geo_accession | GSM298737
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 2 million (25 micron) polystyrene microspheres/100 grams weight at a concentration of 13 million microspheres/ml in 0.01% tween-20.
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "O" for outflow tract.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298737/suppl/GSM298737.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298737/suppl/GSM298737.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
|
GSM298738 | GPL1355 |
|
Week 6 PE2.0 rat, heart right ventricle outflow, biological rep5
|
Gene expression data from right ventricle outflow RNA after 6 weeks of microsphere treatment.
|
strain: Sprague Dawley
gender: male
weight: approximately 350 grams at time of experiment
|
Experimental pulmonary embolism: Week 6 PE2.0 rat, heart right ventricle outflow, biological rep5
|
| Sample_geo_accession | GSM298738
| | Sample_status | Public on Sep 01 2009
| | Sample_submission_date | Jun 17 2008
| | Sample_last_update_date | Aug 10 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rat was injected in the right jugular vein with 2 million (25 micron) polystyrene microspheres/100 grams weight at a concentration of 13 million microspheres/ml in 0.01% tween-20.
| | Sample_growth_protocol_ch1 | Rat was euthanized 6 weeks after vehicle treatment. Heart was excised surgicaly and briefly perfused with saline buffer on a Langendorff heart perfusion apparatus. The right venticle was disected and separated into outflow tract (base) and apex samples. This sample is "O" for outflow tract.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Heart tissue was snap frozen in liquid nitrogen and crushed to a fine powder with a mortar and pestle in liquid nitrogen. Aliquots of powders were homogenized in guanidinium isothiocyanate and extracted with acidic phenol/chloroform. Crude RNA was collected by ethanol precipitation and purified using Qiagen RNeasy spin columns.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to Affymetrix protocols from 5 microgram total RNA (Carolinas Medical Center-modified Affymetrix "Expression Analysis Technical Manual", 2001 using Superscript II cDNA module, Affymetrix IVT module, Affymetrix/Qiagen sample clean-up module).
| | Sample_hyb_protocol | 25 microg of fragmented cRNA was hybridized for 16 hr at 45C on Affymetrix Rat Genome 230 v2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned twice using an Agilent Gene Array Scanner G2500A
| | Sample_data_processing | The data were generated with Affymetrix GeneChip Operating Software using a scaling target of 500, user defined normalization of 1.0, no probe mask, no baseline, and default "parameter" settings.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | John ,,Zagorski
| | Sample_contact_email | john.zagorski@carolinas.org, j_zagorski@hotmail.com
| | Sample_contact_phone | 704-355-3663
| | Sample_contact_laboratory | Cannon Research Center
| | Sample_contact_department | Emergency Medicine
| | Sample_contact_institute | Carolinas Medical Center
| | Sample_contact_address | 1542 Garden Terrace, 301
| | Sample_contact_city | Charlotte
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 28203
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298738/suppl/GSM298738.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM298nnn/GSM298738/suppl/GSM298738.CHP.gz
| | Sample_series_id | GSE11851
| | Sample_data_row_count | 31099
| | |
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