Search results for the GEO ID: GSE11884 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM300153 | GPL1261 |
|
Naive_Furin_Wild-type_1
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Naive_Furin_Wild-type
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mouse T cells
Age: 10-11 weeks
|
Purified naive (CD4+ CD62L+ CD44-) T cells from 10-11 weeks old T cell specific Furin knockout (CD4-cre fur flox/flox) and littermate wild type (fur flox/flox) control mice were profiled for gene expression using Affymetrix MOE 430 2.0 microarray platform.
|
Sample_geo_accession | GSM300153
| Sample_status | Public on Jun 26 2008
| Sample_submission_date | Jun 25 2008
| Sample_last_update_date | Jun 25 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Fresh isolated. Sorted for CD4+ CD62L+ CD44- T cells
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip MOE430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Lai,,Wei
| Sample_contact_email | weil2@mail.nih.gov
| Sample_contact_phone | 3014961480
| Sample_contact_institute | NIH/NEI/NCCAM
| Sample_contact_address | 10 Center Dr. Room 2B47
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM300nnn/GSM300153/suppl/GSM300153.CEL.gz
| Sample_series_id | GSE11884
| Sample_data_row_count | 45101
| |
|
GSM300154 | GPL1261 |
|
Naive_Furin_Wild-type_2
|
Naive_Furin_Wild-type
|
mouse T cells
Age: 10-11 weeks
|
Purified naive (CD4+ CD62L+ CD44-) T cells from 10-11 weeks old T cell specific Furin knockout (CD4-cre fur flox/flox) and littermate wild type (fur flox/flox) control mice were profiled for gene expression using Affymetrix MOE 430 2.0 microarray platform.
|
Sample_geo_accession | GSM300154
| Sample_status | Public on Jun 26 2008
| Sample_submission_date | Jun 25 2008
| Sample_last_update_date | Jun 25 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Fresh isolated. Sorted for CD4+ CD62L+ CD44- T cells
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip MOE430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Lai,,Wei
| Sample_contact_email | weil2@mail.nih.gov
| Sample_contact_phone | 3014961480
| Sample_contact_institute | NIH/NEI/NCCAM
| Sample_contact_address | 10 Center Dr. Room 2B47
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM300nnn/GSM300154/suppl/GSM300154.CEL.gz
| Sample_series_id | GSE11884
| Sample_data_row_count | 45101
| |
|
GSM300155 | GPL1261 |
|
Naive_Furin_Knockout_1
|
Naive_Furin_Knockout
|
mouse T cells
Age: 10-11 weeks
|
Purified naive (CD4+ CD62L+ CD44-) T cells from 10-11 weeks old T cell specific Furin knockout (CD4-cre fur flox/flox) and littermate wild type (fur flox/flox) control mice were profiled for gene expression using Affymetrix MOE 430 2.0 microarray platform.
|
Sample_geo_accession | GSM300155
| Sample_status | Public on Jun 26 2008
| Sample_submission_date | Jun 25 2008
| Sample_last_update_date | Jun 25 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Fresh isolated. Sorted for CD4+ CD62L+ CD44- T cells
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip MOE430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Lai,,Wei
| Sample_contact_email | weil2@mail.nih.gov
| Sample_contact_phone | 3014961480
| Sample_contact_institute | NIH/NEI/NCCAM
| Sample_contact_address | 10 Center Dr. Room 2B47
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM300nnn/GSM300155/suppl/GSM300155.CEL.gz
| Sample_series_id | GSE11884
| Sample_data_row_count | 45101
| |
|
GSM300156 | GPL1261 |
|
Naive_Furin_Knockout_2
|
Naive_Furin_Knockout
|
mouse T cells
Age: 10-11 weeks
|
Purified naive (CD4+ CD62L+ CD44-) T cells from 10-11 weeks old T cell specific Furin knockout (CD4-cre fur flox/flox) and littermate wild type (fur flox/flox) control mice were profiled for gene expression using Affymetrix MOE 430 2.0 microarray platform.
|
Sample_geo_accession | GSM300156
| Sample_status | Public on Jun 26 2008
| Sample_submission_date | Jun 25 2008
| Sample_last_update_date | Jun 25 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Fresh isolated. Sorted for CD4+ CD62L+ CD44- T cells
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip MOE430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Lai,,Wei
| Sample_contact_email | weil2@mail.nih.gov
| Sample_contact_phone | 3014961480
| Sample_contact_institute | NIH/NEI/NCCAM
| Sample_contact_address | 10 Center Dr. Room 2B47
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM300nnn/GSM300156/suppl/GSM300156.CEL.gz
| Sample_series_id | GSE11884
| Sample_data_row_count | 45101
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