Search results for the GEO ID: GSE11924 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM301396 | GPL1261 |
|
Tfh cells Roza1
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CXCR5+ TFH cells isolated from KLH-immunized mice
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strain: C57BL/6 mice
cell type: CXCR5+ TFH cells
|
none
|
Sample_geo_accession | GSM301396
| Sample_status | Public on Jul 03 2008
| Sample_submission_date | Jun 27 2008
| Sample_last_update_date | May 28 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | splenic CD4+CXCR5+ T cells were FACS-sorted 7 days after immunization
| Sample_growth_protocol_ch1 | TFH cells were restimulated in vitro with anti-CD3 for 4 hours before RNA prep
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was prepared using Tri-zol reagent
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Standard Affymetrix IVT labeling protocol. See Affymetrix Expression Analysis Technical Manual
| Sample_hyb_protocol | Following fragmentation, ten micrograms of adjusted cRNA fromeach sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
| Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
| Sample_data_processing | Raw CEL intensity data were GCRMA normalized using R/Bioconductor
| Sample_platform_id | GPL1261
| Sample_contact_name | Chen,,Dong
| Sample_contact_email | cdong@mdanderson.org
| Sample_contact_phone | 713-563-3263
| Sample_contact_fax | 713-563-0604
| Sample_contact_department | Immunology
| Sample_contact_institute | MD Anderson Cancer Center
| Sample_contact_address | 7455 Fannin
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM301nnn/GSM301396/suppl/GSM301396.CEL.gz
| Sample_series_id | GSE11924
| Sample_data_row_count | 45101
| |
|
GSM301397 | GPL1261 |
|
Tfh cells Roza2
|
CXCR5+ TFH cells isolated from KLH-immunized mice
|
strain: C57BL/6
cell type: CXCR5+ TFH cells
|
none
|
Sample_geo_accession | GSM301397
| Sample_status | Public on Jul 03 2008
| Sample_submission_date | Jun 27 2008
| Sample_last_update_date | May 28 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | splenic CD4+CXCR5+ T cells were FACS-sorted 7 days after immunization
| Sample_growth_protocol_ch1 | TFH cells were restimulated in vitro with anti-CD3 for 4 hours before RNA prep
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was prepared using Tri-zol reagent
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Standard Affymetrix IVT labeling protocol. See Affymetrix Expression Analysis Technical Manual
| Sample_hyb_protocol | Following fragmentation, ten micrograms of adjusted cRNA fromeach sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
| Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
| Sample_data_processing | Raw CEL intensity data were GCRMA normalized using R/Bioconductor
| Sample_platform_id | GPL1261
| Sample_contact_name | Chen,,Dong
| Sample_contact_email | cdong@mdanderson.org
| Sample_contact_phone | 713-563-3263
| Sample_contact_fax | 713-563-0604
| Sample_contact_department | Immunology
| Sample_contact_institute | MD Anderson Cancer Center
| Sample_contact_address | 7455 Fannin
| Sample_contact_city | Houston
| Sample_contact_state | TX
| Sample_contact_zip/postal_code | 77030
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM301nnn/GSM301397/suppl/GSM301397.CEL.gz
| Sample_series_id | GSE11924
| Sample_data_row_count | 45101
| |
|
GSM403598 | GPL1261 |
|
Th1 cells 1
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Naïve CD4 T cells were activated with OT-II peptide and APC under Th1 conditions for 5 days
|
strain: C57BL/6
cell type: Th1 cells
|
OT-II Tcr transgenic mice on C57BL/6 background, OT-II peptide and APC, age 6-8 weeks, spleen and limph node CD4 T cells, Th1
Naïve CD4 T cells were activated with OT-II peptide and APC under Th1 conditions for 5 days
|
Sample_geo_accession | GSM403598
| Sample_status | Public on May 28 2009
| Sample_submission_date | May 15 2009
| Sample_last_update_date | May 28 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | the cytokine stimuli for Th1 differentiation were 10 ng/ml of IL-12, 10 mg/ml of anti-IL-4
| Sample_growth_protocol_ch1 | Cells were cultured in RPMI-1640 medium containing 10% FBS, 1 % L-Glu and 1% Penicillin/streptomycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Standard Affymetrix IVT labeling protocol. See Affymetrix Expression Analysis Technical Manual
| Sample_hyb_protocol | Following fragmentation, ten micrograms of adjusted cRNA fromeach sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
| Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
| Sample_data_processing | Raw CEL intensity data were GCRMA normalized using R/Bioconductor
| Sample_platform_id | GPL1261
| Sample_contact_name | Qiang ,,Tian
| Sample_contact_email | qtian@systemsbiology.org
| Sample_contact_phone | 206-732-1308
| Sample_contact_fax | 206-732-1299
| Sample_contact_laboratory | Hood
| Sample_contact_institute | Institute for Systems Biology
| Sample_contact_address | 1441 N 34th St
| Sample_contact_city | Seattle
| Sample_contact_state | WA
| Sample_contact_zip/postal_code | 98115
| Sample_contact_country | USA
| Sample_contact_web_link | www.systemsbiology.org
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM403nnn/GSM403598/suppl/GSM403598.CEL.gz
| Sample_series_id | GSE11924
| Sample_data_row_count | 45101
| |
|
GSM403599 | GPL1261 |
|
Th17 cells 1
|
Naïve CD4 T cells were activated with OT-II peptide and APC under Th17 conditions for 5 days
|
strain: C57BL/6
cell type: Th17 cells
|
OT-II Tcr transgenic mice on C57BL/6 background, OT-II peptide and APC, age 6-8 weeks, spleen and limph node CD4 T cells, Th17
Naïve CD4 T cells were activated with OT-II peptide and APC under Th17 conditions for 5 days
|
Sample_geo_accession | GSM403599
| Sample_status | Public on May 28 2009
| Sample_submission_date | May 15 2009
| Sample_last_update_date | May 28 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | the cytokine stimuli for Th17 differentiation were 20 ng/ml of IL-6, 5 mg/ml TGFb and 10 mg/ml of anti-IL-4 and 10 mg/ml of anti-IFNg
| Sample_growth_protocol_ch1 | Cells were cultured in RPMI-1640 medium containing 10% FBS, 1 % L-Glu and 1% Penicillin/streptomycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Standard Affymetrix IVT labeling protocol. See Affymetrix Expression Analysis Technical Manual
| Sample_hyb_protocol | Following fragmentation, ten micrograms of adjusted cRNA fromeach sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
| Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
| Sample_data_processing | Raw CEL intensity data were GCRMA normalized using R/Bioconductor
| Sample_platform_id | GPL1261
| Sample_contact_name | Qiang ,,Tian
| Sample_contact_email | qtian@systemsbiology.org
| Sample_contact_phone | 206-732-1308
| Sample_contact_fax | 206-732-1299
| Sample_contact_laboratory | Hood
| Sample_contact_institute | Institute for Systems Biology
| Sample_contact_address | 1441 N 34th St
| Sample_contact_city | Seattle
| Sample_contact_state | WA
| Sample_contact_zip/postal_code | 98115
| Sample_contact_country | USA
| Sample_contact_web_link | www.systemsbiology.org
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM403nnn/GSM403599/suppl/GSM403599.CEL.gz
| Sample_series_id | GSE11924
| Sample_data_row_count | 45101
| |
|
GSM403600 | GPL1261 |
|
Th1 cells 2
|
Naïve CD4 T cells were activated with OT-II peptide and APC under Th1 conditions for 5 days
|
strain: C57BL/6
cell type: Th1 cells
|
OT-II Tcr transgenic mice on C57BL/6 background, OT-II peptide and APC, age 6-8 weeks, spleen and limph node CD4 T cells, Th1
Naïve CD4 T cells were activated with OT-II peptide and APC under Th1 conditions for 5 days
|
Sample_geo_accession | GSM403600
| Sample_status | Public on May 28 2009
| Sample_submission_date | May 15 2009
| Sample_last_update_date | May 28 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | the cytokine stimuli for Th1 differentiation were 10 ng/ml of IL-12, 10 mg/ml of anti-IL-4
| Sample_growth_protocol_ch1 | Cells were cultured in RPMI-1640 medium containing 10% FBS, 1 % L-Glu and 1% Penicillin/streptomycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Standard Affymetrix IVT labeling protocol. See Affymetrix Expression Analysis Technical Manual
| Sample_hyb_protocol | Following fragmentation, ten micrograms of adjusted cRNA fromeach sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
| Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
| Sample_data_processing | Raw CEL intensity data were GCRMA normalized using R/Bioconductor
| Sample_platform_id | GPL1261
| Sample_contact_name | Qiang ,,Tian
| Sample_contact_email | qtian@systemsbiology.org
| Sample_contact_phone | 206-732-1308
| Sample_contact_fax | 206-732-1299
| Sample_contact_laboratory | Hood
| Sample_contact_institute | Institute for Systems Biology
| Sample_contact_address | 1441 N 34th St
| Sample_contact_city | Seattle
| Sample_contact_state | WA
| Sample_contact_zip/postal_code | 98115
| Sample_contact_country | USA
| Sample_contact_web_link | www.systemsbiology.org
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM403nnn/GSM403600/suppl/GSM403600.CEL.gz
| Sample_series_id | GSE11924
| Sample_data_row_count | 45101
| |
|
GSM403601 | GPL1261 |
|
Th2 cells 1
|
Naïve CD4 T cells were activated with OT-II peptide and APC under Th2 conditions for 5 days
|
strain: C57BL/6
cell type: Th2 cells
|
OT-II Tcr transgenic mice on C57BL/6 background, OT-II peptide and APC, age 6-8 weeks, spleen and limph node CD4 T cells, Th2
Naïve CD4 T cells were activated with OT-II peptide and APC under Th2 conditions for 5 days
|
Sample_geo_accession | GSM403601
| Sample_status | Public on May 28 2009
| Sample_submission_date | May 15 2009
| Sample_last_update_date | May 28 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | the cytokine stimuli for Th2 differentiation were 10 ng/ml of IL-4, 10 mg/ml of anti-IFNg
| Sample_growth_protocol_ch1 | Cells were cultured in RPMI-1640 medium containing 10% FBS, 1 % L-Glu and 1% Penicillin/streptomycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Standard Affymetrix IVT labeling protocol. See Affymetrix Expression Analysis Technical Manual
| Sample_hyb_protocol | Following fragmentation, ten micrograms of adjusted cRNA fromeach sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
| Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
| Sample_data_processing | Raw CEL intensity data were GCRMA normalized using R/Bioconductor
| Sample_platform_id | GPL1261
| Sample_contact_name | Qiang ,,Tian
| Sample_contact_email | qtian@systemsbiology.org
| Sample_contact_phone | 206-732-1308
| Sample_contact_fax | 206-732-1299
| Sample_contact_laboratory | Hood
| Sample_contact_institute | Institute for Systems Biology
| Sample_contact_address | 1441 N 34th St
| Sample_contact_city | Seattle
| Sample_contact_state | WA
| Sample_contact_zip/postal_code | 98115
| Sample_contact_country | USA
| Sample_contact_web_link | www.systemsbiology.org
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM403nnn/GSM403601/suppl/GSM403601.CEL.gz
| Sample_series_id | GSE11924
| Sample_data_row_count | 45101
| |
|
GSM403602 | GPL1261 |
|
Th2 cells 2
|
Naïve CD4 T cells were activated with OT-II peptide and APC under Th2 conditions for 5 days
|
strain: C57BL/6
cell type: Th2 cells
|
OT-II Tcr transgenic mice on C57BL/6 background, OT-II peptide and APC, age 6-8 weeks, spleen and limph node CD4 T cells, Th2
Naïve CD4 T cells were activated with OT-II peptide and APC under Th2 conditions for 5 days
|
Sample_geo_accession | GSM403602
| Sample_status | Public on May 28 2009
| Sample_submission_date | May 15 2009
| Sample_last_update_date | May 28 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | the cytokine stimuli for Th2 differentiation were 10 ng/ml of IL-4, 10 mg/ml of anti-IFNg
| Sample_growth_protocol_ch1 | Cells were cultured in RPMI-1640 medium containing 10% FBS, 1 % L-Glu and 1% Penicillin/streptomycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Standard Affymetrix IVT labeling protocol. See Affymetrix Expression Analysis Technical Manual
| Sample_hyb_protocol | Following fragmentation, ten micrograms of adjusted cRNA fromeach sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
| Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
| Sample_data_processing | Raw CEL intensity data were GCRMA normalized using R/Bioconductor
| Sample_platform_id | GPL1261
| Sample_contact_name | Qiang ,,Tian
| Sample_contact_email | qtian@systemsbiology.org
| Sample_contact_phone | 206-732-1308
| Sample_contact_fax | 206-732-1299
| Sample_contact_laboratory | Hood
| Sample_contact_institute | Institute for Systems Biology
| Sample_contact_address | 1441 N 34th St
| Sample_contact_city | Seattle
| Sample_contact_state | WA
| Sample_contact_zip/postal_code | 98115
| Sample_contact_country | USA
| Sample_contact_web_link | www.systemsbiology.org
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM403nnn/GSM403602/suppl/GSM403602.CEL.gz
| Sample_series_id | GSE11924
| Sample_data_row_count | 45101
| |
|
GSM403603 | GPL1261 |
|
Th17 cells 2
|
Naïve CD4 T cells were activated with OT-II peptide and APC under Th17 conditions for 5 days
|
strain: C57BL/6
cell type: Th17 cells
|
OT-II Tcr transgenic mice on C57BL/6 background, OT-II peptide and APC, age 6-8 weeks, spleen and limph node CD4 T cells, Th17
Naïve CD4 T cells were activated with OT-II peptide and APC under Th17 conditions for 5 days
|
Sample_geo_accession | GSM403603
| Sample_status | Public on May 28 2009
| Sample_submission_date | May 15 2009
| Sample_last_update_date | May 28 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | the cytokine stimuli for Th17 differentiation were 20 ng/ml of IL-6, 5 mg/ml TGFb and 10 mg/ml of anti-IL-4 and 10 mg/ml of anti-IFNg
| Sample_growth_protocol_ch1 | Cells were cultured in RPMI-1640 medium containing 10% FBS, 1 % L-Glu and 1% Penicillin/streptomycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Standard Affymetrix IVT labeling protocol. See Affymetrix Expression Analysis Technical Manual
| Sample_hyb_protocol | Following fragmentation, ten micrograms of adjusted cRNA fromeach sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
| Sample_scan_protocol | Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
| Sample_data_processing | Raw CEL intensity data were GCRMA normalized using R/Bioconductor
| Sample_platform_id | GPL1261
| Sample_contact_name | Qiang ,,Tian
| Sample_contact_email | qtian@systemsbiology.org
| Sample_contact_phone | 206-732-1308
| Sample_contact_fax | 206-732-1299
| Sample_contact_laboratory | Hood
| Sample_contact_institute | Institute for Systems Biology
| Sample_contact_address | 1441 N 34th St
| Sample_contact_city | Seattle
| Sample_contact_state | WA
| Sample_contact_zip/postal_code | 98115
| Sample_contact_country | USA
| Sample_contact_web_link | www.systemsbiology.org
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM403nnn/GSM403603/suppl/GSM403603.CEL.gz
| Sample_series_id | GSE11924
| Sample_data_row_count | 45101
| |
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