Search results for the GEO ID: GSE12034 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM304260 | GPL570 |
|
human oocyte, biological rep1
|
Pool of 10 human oocytes
|
Genotype: Human Oocytes from reproductively healthy individuals with regular ovulatory cycles, male factor as the only cause for infertility
Age: < 35 yrs.
|
Gene expression data from human oocyte.
|
Sample_geo_accession | GSM304260
| Sample_status | Public on Jul 09 2008
| Sample_submission_date | Jul 08 2008
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Ovarian stimulation was performed under Gn-RH analog suppression [leuprolide acetate (Lupron); Abbott, Abbott Park, IL] in a daily s.c. dose of 0.5 mg. Recombinant FSH (rFSH, Gonal-f-Serono or Puregon; Organon, Roseland, NJ) was administered in daily doses that ranged between 200 and 300 units, starting the second day of the mense. Follicular growth and estradiol levels were monitored every 2 to 3 days until follicles had a mean diameter between 18 and 20 mm. Oocyte maturation was achieved by an injection of 10,000 units of hCG (Pregnyl; Organon). Oocytes were retrieved from the ovary by aspiration using guided transvaginal ultrasound 36 h after hCG administration.
| Sample_growth_protocol_ch1 | Three hours after retrieval, oocytes were denuded from surrounding corona and cumulus cells by a brief incubation (10-30 s) in 80 units/ml hyaluronidase solution (LifeGlobal, Guilford, CT) and subsequent pipetting to completely eliminate other cells. Oocytes were then observed at high magnification to confirm maturity (metaphase II stage) and to confirm the absence of other cells. Each oocyte was rinsed in sterile PBS and lysed in 100 ml of extraction buffer (XB, Arcturus, Mountain View, CA) in an RNase/DNase/Pyrogen-free 0.5-ml microcentrifuge tube. Each sample was incubated for 30 min at 42°C, centrifuged at 3,000 ´ g for 2 min, and stored in liquid nitrogen until use.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated following the guanidium thiocyanate method by using the PicoPure RNA isolation kit (Arcturus, Sunnyvale, CA) following the manufacturer's instructions. However, only 6.5 ?l of elution buffer (Arcturus) was used, and the elution was repeated at least three times by using the first eluate. All RNA samples within the purification column were treated with the RNase-Free DNase (Qiagen, Valencia, CA). Extracted RNA was stored at ?80°C until used as template for cDNA synthesis.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Hybridization cocktails of 300 ml containing 15 mg of fragmented biotin-labeled aRNA and biotinylated exogenous hybridization controls (50 pM control Oligo B2, Eukaryotic hybridization controls) (BioB at 1.5 pM, BioC at 5 pM, BioD at 25 pM, and CreX at 100 pM), herring sperm DNA (0.1 mg/ml), BSA (0.5 mg/ml) in buffer (100 mM Mes/1M NaCl/20 mM EDTA/0.01% Tween 20) were hybridized to the GeneChip Human Genome U133 plus 2.0 array (Affymetrix, Santa Clara, CA). Hybridizations were performed automatically, and each array was prehybridized with all components except the fragmented biotin-labeled aRNA in a chamber at 45°C for 15 min with rotation at 60 rpm. The prehybridized array was then hybridized with the aRNA mixture for 16 h under the prehybridization conditions.
| Sample_scan_protocol | The arrays were scanned using Affymetrix's high density GeneArray Scanner 3000 and imaged using Affymetrix GeneChip Operating Software (GCOS).
| Sample_data_processing | The data were analyzed with dChip using smoothing spline invariant set method with the model based expression algorithm following the perfect match (PM)-mismatch (MM) difference model. In the dChip analysis, array outliers are not treated as missing expression values and no truncation on signal values is applied.
| Sample_platform_id | GPL570
| Sample_contact_name | Arif,Murat,Kocabas
| Sample_contact_email | kocabas@msu.edu, hotu@bidmc.harvard.edu
| Sample_contact_phone | 517-432-8254
| Sample_contact_fax | 517-432-8742
| Sample_contact_laboratory | Cellular Reprogramming Laboratory
| Sample_contact_department | Animal Science
| Sample_contact_institute | Michigan State University
| Sample_contact_address | B270 Anthony Hall
| Sample_contact_city | East Lansing
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48824
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM304nnn/GSM304260/suppl/GSM304260.CEL.gz
| Sample_relation | Reanalyzed by: GSM871485
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE12034
| Sample_data_row_count | 54675
| |
|
GSM304261 | GPL570 |
|
human oocyte, biological rep2
|
Pool of 10 human oocytes
|
Genotype: Human Oocytes from reproductively healthy individuals with regular ovulatory cycles, male factor as the only cause for infertility
Age: < 35 yrs.
|
Gene expression data from human oocyte.
|
Sample_geo_accession | GSM304261
| Sample_status | Public on Jul 09 2008
| Sample_submission_date | Jul 08 2008
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Ovarian stimulation was performed under Gn-RH analog suppression [leuprolide acetate (Lupron); Abbott, Abbott Park, IL] in a daily s.c. dose of 0.5 mg. Recombinant FSH (rFSH, Gonal-f-Serono or Puregon; Organon, Roseland, NJ) was administered in daily doses that ranged between 200 and 300 units, starting the second day of the mense. Follicular growth and estradiol levels were monitored every 2 to 3 days until follicles had a mean diameter between 18 and 20 mm. Oocyte maturation was achieved by an injection of 10,000 units of hCG (Pregnyl; Organon). Oocytes were retrieved from the ovary by aspiration using guided transvaginal ultrasound 36 h after hCG administration.
| Sample_growth_protocol_ch1 | Three hours after retrieval, oocytes were denuded from surrounding corona and cumulus cells by a brief incubation (10-30 s) in 80 units/ml hyaluronidase solution (LifeGlobal, Guilford, CT) and subsequent pipetting to completely eliminate other cells. Oocytes were then observed at high magnification to confirm maturity (metaphase II stage) and to confirm the absence of other cells. Each oocyte was rinsed in sterile PBS and lysed in 100 ml of extraction buffer (XB, Arcturus, Mountain View, CA) in an RNase/DNase/Pyrogen-free 0.5-ml microcentrifuge tube. Each sample was incubated for 30 min at 42°C, centrifuged at 3,000 ´ g for 2 min, and stored in liquid nitrogen until use.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated following the guanidium thiocyanate method by using the PicoPure RNA isolation kit (Arcturus, Sunnyvale, CA) following the manufacturer's instructions. However, only 6.5 ?l of elution buffer (Arcturus) was used, and the elution was repeated at least three times by using the first eluate. All RNA samples within the purification column were treated with the RNase-Free DNase (Qiagen, Valencia, CA). Extracted RNA was stored at ?80°C until used as template for cDNA synthesis.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Hybridization cocktails of 300 ml containing 15 mg of fragmented biotin-labeled aRNA and biotinylated exogenous hybridization controls (50 pM control Oligo B2, Eukaryotic hybridization controls) (BioB at 1.5 pM, BioC at 5 pM, BioD at 25 pM, and CreX at 100 pM), herring sperm DNA (0.1 mg/ml), BSA (0.5 mg/ml) in buffer (100 mM Mes/1M NaCl/20 mM EDTA/0.01% Tween 20) were hybridized to the GeneChip Human Genome U133 plus 2.0 array (Affymetrix, Santa Clara, CA). Hybridizations were performed automatically, and each array was prehybridized with all components except the fragmented biotin-labeled aRNA in a chamber at 45°C for 15 min with rotation at 60 rpm. The prehybridized array was then hybridized with the aRNA mixture for 16 h under the prehybridization conditions.
| Sample_scan_protocol | The arrays were scanned using Affymetrix's high density GeneArray Scanner 3000 and imaged using Affymetrix GeneChip Operating Software (GCOS).
| Sample_data_processing | The data were analyzed with dChip using smoothing spline invariant set method with the model based expression algorithm following the perfect match (PM)-mismatch (MM) difference model. In the dChip analysis, array outliers are not treated as missing expression values and no truncation on signal values is applied.
| Sample_platform_id | GPL570
| Sample_contact_name | Arif,Murat,Kocabas
| Sample_contact_email | kocabas@msu.edu, hotu@bidmc.harvard.edu
| Sample_contact_phone | 517-432-8254
| Sample_contact_fax | 517-432-8742
| Sample_contact_laboratory | Cellular Reprogramming Laboratory
| Sample_contact_department | Animal Science
| Sample_contact_institute | Michigan State University
| Sample_contact_address | B270 Anthony Hall
| Sample_contact_city | East Lansing
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48824
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM304nnn/GSM304261/suppl/GSM304261.CEL.gz
| Sample_relation | Reanalyzed by: GSM871486
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE12034
| Sample_data_row_count | 54675
| |
|
GSM304262 | GPL570 |
|
human oocyte, biological rep3
|
Pool of 10 human oocytes
|
Genotype: Human Oocytes from reproductively healthy individuals with regular ovulatory cycles, male factor as the only cause for infertility
Age: < 35 yrs.
|
Gene expression data from human oocyte.
|
Sample_geo_accession | GSM304262
| Sample_status | Public on Jul 09 2008
| Sample_submission_date | Jul 08 2008
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Ovarian stimulation was performed under Gn-RH analog suppression [leuprolide acetate (Lupron); Abbott, Abbott Park, IL] in a daily s.c. dose of 0.5 mg. Recombinant FSH (rFSH, Gonal-f-Serono or Puregon; Organon, Roseland, NJ) was administered in daily doses that ranged between 200 and 300 units, starting the second day of the mense. Follicular growth and estradiol levels were monitored every 2 to 3 days until follicles had a mean diameter between 18 and 20 mm. Oocyte maturation was achieved by an injection of 10,000 units of hCG (Pregnyl; Organon). Oocytes were retrieved from the ovary by aspiration using guided transvaginal ultrasound 36 h after hCG administration.
| Sample_growth_protocol_ch1 | Three hours after retrieval, oocytes were denuded from surrounding corona and cumulus cells by a brief incubation (10-30 s) in 80 units/ml hyaluronidase solution (LifeGlobal, Guilford, CT) and subsequent pipetting to completely eliminate other cells. Oocytes were then observed at high magnification to confirm maturity (metaphase II stage) and to confirm the absence of other cells. Each oocyte was rinsed in sterile PBS and lysed in 100 ml of extraction buffer (XB, Arcturus, Mountain View, CA) in an RNase/DNase/Pyrogen-free 0.5-ml microcentrifuge tube. Each sample was incubated for 30 min at 42°C, centrifuged at 3,000 ´ g for 2 min, and stored in liquid nitrogen until use.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated following the guanidium thiocyanate method by using the PicoPure RNA isolation kit (Arcturus, Sunnyvale, CA) following the manufacturer's instructions. However, only 6.5 ?l of elution buffer (Arcturus) was used, and the elution was repeated at least three times by using the first eluate. All RNA samples within the purification column were treated with the RNase-Free DNase (Qiagen, Valencia, CA). Extracted RNA was stored at ?80°C until used as template for cDNA synthesis.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Hybridization cocktails of 300 ml containing 15 mg of fragmented biotin-labeled aRNA and biotinylated exogenous hybridization controls (50 pM control Oligo B2, Eukaryotic hybridization controls) (BioB at 1.5 pM, BioC at 5 pM, BioD at 25 pM, and CreX at 100 pM), herring sperm DNA (0.1 mg/ml), BSA (0.5 mg/ml) in buffer (100 mM Mes/1M NaCl/20 mM EDTA/0.01% Tween 20) were hybridized to the GeneChip Human Genome U133 plus 2.0 array (Affymetrix, Santa Clara, CA). Hybridizations were performed automatically, and each array was prehybridized with all components except the fragmented biotin-labeled aRNA in a chamber at 45°C for 15 min with rotation at 60 rpm. The prehybridized array was then hybridized with the aRNA mixture for 16 h under the prehybridization conditions.
| Sample_scan_protocol | The arrays were scanned using Affymetrix's high density GeneArray Scanner 3000 and imaged using Affymetrix GeneChip Operating Software (GCOS).
| Sample_data_processing | The data were analyzed with dChip using smoothing spline invariant set method with the model based expression algorithm following the perfect match (PM)-mismatch (MM) difference model. In the dChip analysis, array outliers are not treated as missing expression values and no truncation on signal values is applied.
| Sample_platform_id | GPL570
| Sample_contact_name | Arif,Murat,Kocabas
| Sample_contact_email | kocabas@msu.edu, hotu@bidmc.harvard.edu
| Sample_contact_phone | 517-432-8254
| Sample_contact_fax | 517-432-8742
| Sample_contact_laboratory | Cellular Reprogramming Laboratory
| Sample_contact_department | Animal Science
| Sample_contact_institute | Michigan State University
| Sample_contact_address | B270 Anthony Hall
| Sample_contact_city | East Lansing
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48824
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM304nnn/GSM304262/suppl/GSM304262.CEL.gz
| Sample_relation | Reanalyzed by: GSM871487
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE12034
| Sample_data_row_count | 54675
| |
|
GSM304263 | GPL570 |
|
human reference, biological rep1
|
Pool of ten normal human tissues for reference
|
Genotype: 10 different normal human tissues, including skeletal muscle, kidney, lung, colon, liver, spleen, breast, brain, heart, and stomach.
Age: N/A
|
Gene expression data from pool of 10 normal human tissue.
|
Sample_geo_accession | GSM304263
| Sample_status | Public on Jul 09 2008
| Sample_submission_date | Jul 08 2008
| Sample_last_update_date | Jul 14 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Samples are obtained from Ambion
| Sample_growth_protocol_ch1 | Samples are obtained from Ambion.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The samples are processed using Ambion's RNA isolation reagents to produce highly pure, intact RNA. A stringent DNase treatment is performed to ensure that the RNA is ready for use in any downstream application, including RT-PCR.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Hybridization cocktails of 300 ml containing 15 mg of fragmented biotin-labeled aRNA and biotinylated exogenous hybridization controls (50 pM control Oligo B2, Eukaryotic hybridization controls) (BioB at 1.5 pM, BioC at 5 pM, BioD at 25 pM, and CreX at 100 pM), herring sperm DNA (0.1 mg/ml), BSA (0.5 mg/ml) in buffer (100 mM Mes/1M NaCl/20 mM EDTA/0.01% Tween 20) were hybridized to the GeneChip Human Genome U133 plus 2.0 array (Affymetrix, Santa Clara, CA). Hybridizations were performed automatically, and each array was prehybridized with all components except the fragmented biotin-labeled aRNA in a chamber at 45°C for 15 min with rotation at 60 rpm. The prehybridized array was then hybridized with the aRNA mixture for 16 h under the prehybridization conditions.
| Sample_scan_protocol | The arrays were scanned using Affymetrix's high density GeneArray Scanner 3000 and imaged using Affymetrix GeneChip Operating Software (GCOS).
| Sample_data_processing | The data were analyzed with dChip using smoothing spline invariant set method with the model based expression algorithm following the perfect match (PM)-mismatch (MM) difference model. In the dChip analysis, array outliers are not treated as missing expression values and no truncation on signal values is applied.
| Sample_platform_id | GPL570
| Sample_contact_name | Arif,Murat,Kocabas
| Sample_contact_email | kocabas@msu.edu, hotu@bidmc.harvard.edu
| Sample_contact_phone | 517-432-8254
| Sample_contact_fax | 517-432-8742
| Sample_contact_laboratory | Cellular Reprogramming Laboratory
| Sample_contact_department | Animal Science
| Sample_contact_institute | Michigan State University
| Sample_contact_address | B270 Anthony Hall
| Sample_contact_city | East Lansing
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48824
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM304nnn/GSM304263/suppl/GSM304263.CEL.gz
| Sample_series_id | GSE12034
| Sample_data_row_count | 54675
| |
|
GSM304264 | GPL570 |
|
human reference, biological rep2
|
Pool of ten normal human tissues for reference
|
Genotype: 10 different normal human tissues, including skeletal muscle, kidney, lung, colon, liver, spleen, breast, brain, heart, and stomach.
Age: N/A
|
Gene expression data from pool of 10 normal human tissue.
|
Sample_geo_accession | GSM304264
| Sample_status | Public on Jul 09 2008
| Sample_submission_date | Jul 08 2008
| Sample_last_update_date | Jul 14 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Samples are obtained from Ambion
| Sample_growth_protocol_ch1 | Samples are obtained from Ambion.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The samples are processed using Ambion's RNA isolation reagents to produce highly pure, intact RNA. A stringent DNase treatment is performed to ensure that the RNA is ready for use in any downstream application, including RT-PCR.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Hybridization cocktails of 300 ml containing 15 mg of fragmented biotin-labeled aRNA and biotinylated exogenous hybridization controls (50 pM control Oligo B2, Eukaryotic hybridization controls) (BioB at 1.5 pM, BioC at 5 pM, BioD at 25 pM, and CreX at 100 pM), herring sperm DNA (0.1 mg/ml), BSA (0.5 mg/ml) in buffer (100 mM Mes/1M NaCl/20 mM EDTA/0.01% Tween 20) were hybridized to the GeneChip Human Genome U133 plus 2.0 array (Affymetrix, Santa Clara, CA). Hybridizations were performed automatically, and each array was prehybridized with all components except the fragmented biotin-labeled aRNA in a chamber at 45°C for 15 min with rotation at 60 rpm. The prehybridized array was then hybridized with the aRNA mixture for 16 h under the prehybridization conditions.
| Sample_scan_protocol | The arrays were scanned using Affymetrix's high density GeneArray Scanner 3000 and imaged using Affymetrix GeneChip Operating Software (GCOS).
| Sample_data_processing | The data were analyzed with dChip using smoothing spline invariant set method with the model based expression algorithm following the perfect match (PM)-mismatch (MM) difference model. In the dChip analysis, array outliers are not treated as missing expression values and no truncation on signal values is applied.
| Sample_platform_id | GPL570
| Sample_contact_name | Arif,Murat,Kocabas
| Sample_contact_email | kocabas@msu.edu, hotu@bidmc.harvard.edu
| Sample_contact_phone | 517-432-8254
| Sample_contact_fax | 517-432-8742
| Sample_contact_laboratory | Cellular Reprogramming Laboratory
| Sample_contact_department | Animal Science
| Sample_contact_institute | Michigan State University
| Sample_contact_address | B270 Anthony Hall
| Sample_contact_city | East Lansing
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48824
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM304nnn/GSM304264/suppl/GSM304264.CEL.gz
| Sample_series_id | GSE12034
| Sample_data_row_count | 54675
| |
|
GSM304265 | GPL570 |
|
human reference, biological rep3
|
Pool of ten normal human tissues for reference
|
Genotype: 10 different normal human tissues, including skeletal muscle, kidney, lung, colon, liver, spleen, breast, brain, heart, and stomach.
Age: N/A
|
Gene expression data from pool of 10 normal human tissue.
|
Sample_geo_accession | GSM304265
| Sample_status | Public on Jul 09 2008
| Sample_submission_date | Jul 08 2008
| Sample_last_update_date | Jul 14 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Samples are obtained from Ambion
| Sample_growth_protocol_ch1 | Samples are obtained from Ambion.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | The samples are processed using Ambion's RNA isolation reagents to produce highly pure, intact RNA. A stringent DNase treatment is performed to ensure that the RNA is ready for use in any downstream application, including RT-PCR.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Hybridization cocktails of 300 ml containing 15 mg of fragmented biotin-labeled aRNA and biotinylated exogenous hybridization controls (50 pM control Oligo B2, Eukaryotic hybridization controls) (BioB at 1.5 pM, BioC at 5 pM, BioD at 25 pM, and CreX at 100 pM), herring sperm DNA (0.1 mg/ml), BSA (0.5 mg/ml) in buffer (100 mM Mes/1M NaCl/20 mM EDTA/0.01% Tween 20) were hybridized to the GeneChip Human Genome U133 plus 2.0 array (Affymetrix, Santa Clara, CA). Hybridizations were performed automatically, and each array was prehybridized with all components except the fragmented biotin-labeled aRNA in a chamber at 45°C for 15 min with rotation at 60 rpm. The prehybridized array was then hybridized with the aRNA mixture for 16 h under the prehybridization conditions.
| Sample_scan_protocol | The arrays were scanned using Affymetrix's high density GeneArray Scanner 3000 and imaged using Affymetrix GeneChip Operating Software (GCOS).
| Sample_data_processing | The data were analyzed with dChip using smoothing spline invariant set method with the model based expression algorithm following the perfect match (PM)-mismatch (MM) difference model. In the dChip analysis, array outliers are not treated as missing expression values and no truncation on signal values is applied.
| Sample_platform_id | GPL570
| Sample_contact_name | Arif,Murat,Kocabas
| Sample_contact_email | kocabas@msu.edu, hotu@bidmc.harvard.edu
| Sample_contact_phone | 517-432-8254
| Sample_contact_fax | 517-432-8742
| Sample_contact_laboratory | Cellular Reprogramming Laboratory
| Sample_contact_department | Animal Science
| Sample_contact_institute | Michigan State University
| Sample_contact_address | B270 Anthony Hall
| Sample_contact_city | East Lansing
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48824
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM304nnn/GSM304265/suppl/GSM304265.CEL.gz
| Sample_series_id | GSE12034
| Sample_data_row_count | 54675
| |
|
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