Search results for the GEO ID: GSE12131  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM305784 | GPL570 |
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HUVECs Non intoxicated Rep1
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HUVEC control monolayer
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tissue: primary human umbilical vein endothelial cells (HUVECs)
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Experiments performed by AROS Applied biotechnology
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| Sample_geo_accession | GSM305784
| | Sample_status | Public on Jan 26 2010
| | Sample_submission_date | Jul 16 2008
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | PromoCell, Heidelberg, Germany
| | Sample_treatment_protocol_ch1 | control condition
| | Sample_growth_protocol_ch1 | HUVECs (PromoCell, Heidelberg, Germany) were cultured in human endothelial SFM supplemented with 20 % FBS, 20 ng/ml bFGF, 10 ng/ml EGF (Invitrogen) and 1 microg/ml heparin (Sigma).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy MiniKit (Qiagen, Valencia, CA). Both integrity and purity of RNA were checked using the Bioanalyzer 2100 and RNA 6000 LabChip kit from Agilent Technologies (Palo Alto, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard affymetrix protocol
| | Sample_hyb_protocol | standard affymetrix protocol
| | Sample_scan_protocol | The probe arrays were scanned at 560 nm using a confocal laser-scanning microscope (Hewlett Packard GeneArray Scanner G2500A). The readings from the quantitative scanning were analysed by the Affymetrix Gene Expression Analysis Software 5.0 (MAS 5.0).
| | Sample_data_processing | Data from the individual samples was subjected to Pairwise comparison analyses in the Software MAS 5.0, based on empirical algorithms as described in Affymetrix's statistical reference guide. standard affymetrix protocol. Briefly, the Change Algorithm indicated a change in transcript level between a baseline and an experiment array, further indicating an increase, marginal increase, decrease, marginal decrease or no change in gene expression. On the other hand, the Signal Log Ratio (SLR) Algorithm estimated the magnitude and direction of change of a transcript when two arrays were compared (experiment versus baseline), expressed as the log2 ratio.
| | Sample_platform_id | GPL570
| | Sample_contact_name | emmanuel,,lemichez
| | Sample_contact_email | lemichez@unice.fr
| | Sample_contact_phone | 0489064261
| | Sample_contact_laboratory | INSERM U895
| | Sample_contact_department | Microbiology
| | Sample_contact_institute | INSERM
| | Sample_contact_address | 151 route Saint Antoine de Ginestière
| | Sample_contact_city | Nice
| | Sample_contact_zip/postal_code | 06204 NICE CEDEX 3
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM305nnn/GSM305784/suppl/GSM305784.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM305nnn/GSM305784/suppl/GSM305784.CHP.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE12131
| | Sample_data_row_count | 54675
| | |
|
GSM305785 | GPL570 |
|
HUVECs Non intoxicated Rep2
|
HUVEC control monolayer
|
tissue: primary human umbilical vein endothelial cells (HUVECs)
|
Experiments performed by AROS Applied biotechnology
|
| Sample_geo_accession | GSM305785
| | Sample_status | Public on Jan 26 2010
| | Sample_submission_date | Jul 16 2008
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | PromoCell, Heidelberg, Germany
| | Sample_treatment_protocol_ch1 | control condition
| | Sample_growth_protocol_ch1 | HUVECs (PromoCell, Heidelberg, Germany) were cultured in human endothelial SFM supplemented with 20 % FBS, 20 ng/ml bFGF, 10 ng/ml EGF (Invitrogen) and 1 microg/ml heparin (Sigma).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy MiniKit (Qiagen, Valencia, CA). Both integrity and purity of RNA were checked using the Bioanalyzer 2100 and RNA 6000 LabChip kit from Agilent Technologies (Palo Alto, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard affymetrix protocol
| | Sample_hyb_protocol | standard affymetrix protocol
| | Sample_scan_protocol | The probe arrays were scanned at 560 nm using a confocal laser-scanning microscope (Hewlett Packard GeneArray Scanner G2500A). The readings from the quantitative scanning were analysed by the Affymetrix Gene Expression Analysis Software 5.0 (MAS 5.0).
| | Sample_data_processing | Data from the individual samples was subjected to Pairwise comparison analyses in the Software MAS 5.0, based on empirical algorithms as described in Affymetrix's statistical reference guide. standard affymetrix protocol. Briefly, the Change Algorithm indicated a change in transcript level between a baseline and an experiment array, further indicating an increase, marginal increase, decrease, marginal decrease or no change in gene expression. On the other hand, the Signal Log Ratio (SLR) Algorithm estimated the magnitude and direction of change of a transcript when two arrays were compared (experiment versus baseline), expressed as the log2 ratio.
| | Sample_platform_id | GPL570
| | Sample_contact_name | emmanuel,,lemichez
| | Sample_contact_email | lemichez@unice.fr
| | Sample_contact_phone | 0489064261
| | Sample_contact_laboratory | INSERM U895
| | Sample_contact_department | Microbiology
| | Sample_contact_institute | INSERM
| | Sample_contact_address | 151 route Saint Antoine de Ginestière
| | Sample_contact_city | Nice
| | Sample_contact_zip/postal_code | 06204 NICE CEDEX 3
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM305nnn/GSM305785/suppl/GSM305785.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM305nnn/GSM305785/suppl/GSM305785.CHP.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE12131
| | Sample_data_row_count | 54675
| | |
|
GSM305786 | GPL570 |
|
HUVECs Intoxicated by LT _4h
|
LT-intoxicated HUVEC monolayer, 4h
|
tissue: primary human umbilical vein endothelial cells (HUVECs)
|
Experiments performed by AROS Applied biotechnology
|
| Sample_geo_accession | GSM305786
| | Sample_status | Public on Jan 26 2010
| | Sample_submission_date | Jul 16 2008
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | PromoCell, Heidelberg, Germany
| | Sample_treatment_protocol_ch1 | HUVEC monolayers were treated with LT (PA+LF, 3+1 microg/ml), 4 hours
| | Sample_growth_protocol_ch1 | HUVECs (PromoCell, Heidelberg, Germany) were cultured in human endothelial SFM supplemented with 20 % FBS, 20 ng/ml bFGF, 10 ng/ml EGF (Invitrogen) and 1 microg/ml heparin (Sigma).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy MiniKit (Qiagen, Valencia, CA). Both integrity and purity of RNA were checked using the Bioanalyzer 2100 and RNA 6000 LabChip kit from Agilent Technologies (Palo Alto, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard affymetrix protocol
| | Sample_hyb_protocol | standard affymetrix protocol
| | Sample_scan_protocol | The probe arrays were scanned at 560 nm using a confocal laser-scanning microscope (Hewlett Packard GeneArray Scanner G2500A). The readings from the quantitative scanning were analysed by the Affymetrix Gene Expression Analysis Software 5.0 (MAS 5.0).
| | Sample_data_processing | Data from the individual samples was subjected to Pairwise comparison analyses in the Software MAS 5.0, based on empirical algorithms as described in Affymetrix's statistical reference guide. standard affymetrix protocol. Briefly, the Change Algorithm indicated a change in transcript level between a baseline and an experiment array, further indicating an increase, marginal increase, decrease, marginal decrease or no change in gene expression. On the other hand, the Signal Log Ratio (SLR) Algorithm estimated the magnitude and direction of change of a transcript when two arrays were compared (experiment versus baseline), expressed as the log2 ratio.
| | Sample_platform_id | GPL570
| | Sample_contact_name | emmanuel,,lemichez
| | Sample_contact_email | lemichez@unice.fr
| | Sample_contact_phone | 0489064261
| | Sample_contact_laboratory | INSERM U895
| | Sample_contact_department | Microbiology
| | Sample_contact_institute | INSERM
| | Sample_contact_address | 151 route Saint Antoine de Ginestière
| | Sample_contact_city | Nice
| | Sample_contact_zip/postal_code | 06204 NICE CEDEX 3
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM305nnn/GSM305786/suppl/GSM305786.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM305nnn/GSM305786/suppl/GSM305786.CHP.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE12131
| | Sample_data_row_count | 54675
| | |
|
GSM305787 | GPL570 |
|
HUVECs Intoxicated by LT 8h
|
LT-intoxicated HUVEC monolayer, 8h
|
tissue: primary human umbilical vein endothelial cells (HUVECs)
|
Experiments performed by AROS Applied biotechnology
|
| Sample_geo_accession | GSM305787
| | Sample_status | Public on Jan 26 2010
| | Sample_submission_date | Jul 16 2008
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | PromoCell, Heidelberg, Germany
| | Sample_treatment_protocol_ch1 | HUVEC monolayers were treated with the letal toxin LT of Bacillus anthracis (PA+LF, 3+1 microg/ml), 8 hours
| | Sample_growth_protocol_ch1 | HUVECs (PromoCell, Heidelberg, Germany) were cultured in human endothelial SFM supplemented with 20 % FBS, 20 ng/ml bFGF, 10 ng/ml EGF (Invitrogen) and 1 microg/ml heparin (Sigma).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy MiniKit (Qiagen, Valencia, CA). Both integrity and purity of RNA were checked using the Bioanalyzer 2100 and RNA 6000 LabChip kit from Agilent Technologies (Palo Alto, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | standard affymetrix protocol
| | Sample_hyb_protocol | standard affymetrix protocol
| | Sample_scan_protocol | The probe arrays were scanned at 560 nm using a confocal laser-scanning microscope (Hewlett Packard GeneArray Scanner G2500A). The readings from the quantitative scanning were analysed by the Affymetrix Gene Expression Analysis Software 5.0 (MAS 5.0).
| | Sample_data_processing | Data from the individual samples was subjected to Pairwise comparison analyses in the Software MAS 5.0, based on empirical algorithms as described in Affymetrix's statistical reference guide. standard affymetrix protocol. Briefly, the Change Algorithm indicated a change in transcript level between a baseline and an experiment array, further indicating an increase, marginal increase, decrease, marginal decrease or no change in gene expression. On the other hand, the Signal Log Ratio (SLR) Algorithm estimated the magnitude and direction of change of a transcript when two arrays were compared (experiment versus baseline), expressed as the log2 ratio.
| | Sample_platform_id | GPL570
| | Sample_contact_name | emmanuel,,lemichez
| | Sample_contact_email | lemichez@unice.fr
| | Sample_contact_phone | 0489064261
| | Sample_contact_laboratory | INSERM U895
| | Sample_contact_department | Microbiology
| | Sample_contact_institute | INSERM
| | Sample_contact_address | 151 route Saint Antoine de Ginestière
| | Sample_contact_city | Nice
| | Sample_contact_zip/postal_code | 06204 NICE CEDEX 3
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM305nnn/GSM305787/suppl/GSM305787.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM305nnn/GSM305787/suppl/GSM305787.CHP.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE12131
| | Sample_data_row_count | 54675
| | |
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