Search results for the GEO ID: GSE12142 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM305906 | GPL1261 |
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B16 at doxorubicnie 0h
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B16 Mouse Melanoma Cell at 0h
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C57BL/6
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Gene expression data from murine BMDM
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Sample_geo_accession | GSM305906
| Sample_status | Public on Jul 18 2008
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 17 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | On the experiment day, B16 cells were treated with 1 microgram/ml of doxorubicin.
| Sample_growth_protocol_ch1 | B16 mouse melanoma cells were cultured in DMEM medium containing antibiotics and 10% FBS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetix GeneChip® Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with GCOS 1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Chun,,Kim
| Sample_contact_email | chun.kim@cbrc2.mgh.harvard.edu
| Sample_contact_phone | 617-726-4456
| Sample_contact_fax | 617-726-4453
| Sample_contact_department | Cutaneous Biology Research Center
| Sample_contact_institute | Massachusetts General Hospital
| Sample_contact_address | Bldg 149, 13th Street
| Sample_contact_city | Charlestown
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02129
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM305nnn/GSM305906/suppl/GSM305906.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM305nnn/GSM305906/suppl/GSM305906.CHP.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM305nnn/GSM305906/suppl/GSM305906.EXP.gz
| Sample_series_id | GSE12142
| Sample_data_row_count | 45101
| |
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GSM305907 | GPL1261 |
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B16 at doxorubicine 2h
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B16 Mouse Melanoma Cell at 2h after ET treatment
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C57BL/6
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Gene expression data from murine BMDM treated with ET for 2h
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Sample_geo_accession | GSM305907
| Sample_status | Public on Jul 18 2008
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 17 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | On the experiment day, B16 cells were treated with 1 microgram/ml of doxorubicin.
| Sample_growth_protocol_ch1 | B16 mouse melanoma cells were cultured in DMEM medium containing antibiotics and 10% FBS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetix GeneChip® Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with GCOS 1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Chun,,Kim
| Sample_contact_email | chun.kim@cbrc2.mgh.harvard.edu
| Sample_contact_phone | 617-726-4456
| Sample_contact_fax | 617-726-4453
| Sample_contact_department | Cutaneous Biology Research Center
| Sample_contact_institute | Massachusetts General Hospital
| Sample_contact_address | Bldg 149, 13th Street
| Sample_contact_city | Charlestown
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02129
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM305nnn/GSM305907/suppl/GSM305907.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM305nnn/GSM305907/suppl/GSM305907.CHP.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM305nnn/GSM305907/suppl/GSM305907.EXP.gz
| Sample_series_id | GSE12142
| Sample_data_row_count | 45101
| |
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GSM305908 | GPL1261 |
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B16 at doxorubicine 4h
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B16 Mouse Melanoma Cell at 4h after ET treatment
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C57BL/6
|
Gene expression data from murine BMDM treated with ET for 4h
|
Sample_geo_accession | GSM305908
| Sample_status | Public on Jul 18 2008
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 17 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | On the experiment day, B16 cells were treated with 1 microgram/ml of doxorubicin.
| Sample_growth_protocol_ch1 | B16 mouse melanoma cells were cultured in DMEM medium containing antibiotics and 10% FBS.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetix GeneChip® Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with GCOS 1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1261
| Sample_contact_name | Chun,,Kim
| Sample_contact_email | chun.kim@cbrc2.mgh.harvard.edu
| Sample_contact_phone | 617-726-4456
| Sample_contact_fax | 617-726-4453
| Sample_contact_department | Cutaneous Biology Research Center
| Sample_contact_institute | Massachusetts General Hospital
| Sample_contact_address | Bldg 149, 13th Street
| Sample_contact_city | Charlestown
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02129
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM305nnn/GSM305908/suppl/GSM305908.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM305nnn/GSM305908/suppl/GSM305908.CHP.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM305nnn/GSM305908/suppl/GSM305908.EXP.gz
| Sample_series_id | GSE12142
| Sample_data_row_count | 45101
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