Search results for the GEO ID: GSE12153 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM306043 | GPL1355 |
|
FD19 lung, total RNA, biological replicate 1
|
total RNA from FD19 rat lung
|
Strain: Wistar
|
steady-state expression data from canalicular lung
|
Sample_geo_accession | GSM306043
| Sample_status | Public on Jul 07 2009
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 07 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | lungs excised and snap-frozen in liquid nitrogen
| Sample_growth_protocol_ch1 | fetuses and newborn rats from timed pregnant gestation. Fetuses delivered by Cesarean section. Newborns vaginally delivered.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA by Trizol extraction according to the manufacturer's instructions. Polysomal RNA from sucrose density gradient fractions where n> 7 ribosomes/mRNA, extracted by guanidine-HCl, phenol/CHCl3 extracted, and ethanol precipitated. All further purified with Qiagen RNeasy according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 5 ug RNA using Enzo IVT kit for in vitro transcription
| Sample_hyb_protocol | 15 ug of labeled cRNA were used for hybridization to Affymetrix RAE2302 chips using protocol EukGE-WS2v4
| Sample_scan_protocol | Scanned using Affymetrix GeneChip Scanner 3000
| Sample_data_processing | scaling target signal 150 using GCOS 1.4
| Sample_platform_id | GPL1355
| Sample_contact_name | Gail,,Otulakowski
| Sample_contact_department | Physiology & Experimental Medicine
| Sample_contact_institute | Hospital for Sick Children Research Institute
| Sample_contact_address | 555 University Ave
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM306nnn/GSM306043/suppl/GSM306043.CEL.gz
| Sample_series_id | GSE12153
| Sample_data_row_count | 31099
| |
|
GSM306044 | GPL1355 |
|
FD19 lung, total RNA, biological replicate 2
|
total RNA from FD19 rat lung
|
Strain: Wistar
|
steady-state expression data from canalicular lung
|
Sample_geo_accession | GSM306044
| Sample_status | Public on Jul 07 2009
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 07 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | lungs excised and snap-frozen in liquid nitrogen
| Sample_growth_protocol_ch1 | fetuses and newborn rats from timed pregnant gestation. Fetuses delivered by Cesarean section. Newborns vaginally delivered.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA by Trizol extraction according to the manufacturer's instructions. Polysomal RNA from sucrose density gradient fractions where n> 7 ribosomes/mRNA, extracted by guanidine-HCl, phenol/CHCl3 extracted, and ethanol precipitated. All further purified with Qiagen RNeasy according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 5 ug RNA using Enzo IVT kit for in vitro transcription
| Sample_hyb_protocol | 15 ug of labeled cRNA were used for hybridization to Affymetrix RAE2302 chips using protocol EukGE-WS2v4
| Sample_scan_protocol | Scanned using Affymetrix GeneChip Scanner 3000
| Sample_data_processing | scaling target signal 150 using GCOS 1.4
| Sample_platform_id | GPL1355
| Sample_contact_name | Gail,,Otulakowski
| Sample_contact_department | Physiology & Experimental Medicine
| Sample_contact_institute | Hospital for Sick Children Research Institute
| Sample_contact_address | 555 University Ave
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM306nnn/GSM306044/suppl/GSM306044.CEL.gz
| Sample_series_id | GSE12153
| Sample_data_row_count | 31099
| |
|
GSM306045 | GPL1355 |
|
FD19 lung, total RNA, biological replicate 3
|
total RNA from FD19 rat lung
|
Strain: Wistar
|
steady-state expression data from canalicular lung
|
Sample_geo_accession | GSM306045
| Sample_status | Public on Jul 07 2009
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 07 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | lungs excised and snap-frozen in liquid nitrogen
| Sample_growth_protocol_ch1 | fetuses and newborn rats from timed pregnant gestation. Fetuses delivered by Cesarean section. Newborns vaginally delivered.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA by Trizol extraction according to the manufacturer's instructions. Polysomal RNA from sucrose density gradient fractions where n> 7 ribosomes/mRNA, extracted by guanidine-HCl, phenol/CHCl3 extracted, and ethanol precipitated. All further purified with Qiagen RNeasy according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 5 ug RNA using Enzo IVT kit for in vitro transcription
| Sample_hyb_protocol | 15 ug of labeled cRNA were used for hybridization to Affymetrix RAE2302 chips using protocol EukGE-WS2v4
| Sample_scan_protocol | Scanned using Affymetrix GeneChip Scanner 3000
| Sample_data_processing | scaling target signal 150 using GCOS 1.4
| Sample_platform_id | GPL1355
| Sample_contact_name | Gail,,Otulakowski
| Sample_contact_department | Physiology & Experimental Medicine
| Sample_contact_institute | Hospital for Sick Children Research Institute
| Sample_contact_address | 555 University Ave
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM306nnn/GSM306045/suppl/GSM306045.CEL.gz
| Sample_series_id | GSE12153
| Sample_data_row_count | 31099
| |
|
GSM306046 | GPL1355 |
|
FD19 lung, polysomal RNA, biological replicate 1
|
RNA from large polysomes from FD19 rat lung
|
Strain: Wistar
|
polysome-associated RNA from canalicular lung
|
Sample_geo_accession | GSM306046
| Sample_status | Public on Jul 07 2009
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 07 2009
| Sample_type | other
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | lungs excised and snap-frozen in liquid nitrogen
| Sample_growth_protocol_ch1 | fetuses and newborn rats from timed pregnant gestation. Fetuses delivered by Cesarean section. Newborns vaginally delivered.
| Sample_molecule_ch1 | other
| Sample_extract_protocol_ch1 | Total RNA by Trizol extraction according to the manufacturer's instructions. Polysomal RNA from sucrose density gradient fractions where n> 7 ribosomes/mRNA, extracted by guanidine-HCl, phenol/CHCl3 extracted, and ethanol precipitated. All further purified with Qiagen RNeasy according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 5 ug RNA using Enzo IVT kit for in vitro transcription
| Sample_hyb_protocol | 15 ug of labeled cRNA were used for hybridization to Affymetrix RAE2302 chips using protocol EukGE-WS2v4
| Sample_scan_protocol | Scanned using Affymetrix GeneChip Scanner 3000
| Sample_data_processing | scaling target signal 150 using GCOS 1.4
| Sample_platform_id | GPL1355
| Sample_contact_name | Gail,,Otulakowski
| Sample_contact_department | Physiology & Experimental Medicine
| Sample_contact_institute | Hospital for Sick Children Research Institute
| Sample_contact_address | 555 University Ave
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM306nnn/GSM306046/suppl/GSM306046.CEL.gz
| Sample_series_id | GSE12153
| Sample_data_row_count | 31099
| |
|
GSM306047 | GPL1355 |
|
FD19 lung, polysomal RNA, biological replicate 2
|
RNA from large polysomes from FD19 rat lung
|
Strain: Wistar
|
polysome-associated RNA from canalicular lung
|
Sample_geo_accession | GSM306047
| Sample_status | Public on Jul 07 2009
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 07 2009
| Sample_type | other
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | lungs excised and snap-frozen in liquid nitrogen
| Sample_growth_protocol_ch1 | fetuses and newborn rats from timed pregnant gestation. Fetuses delivered by Cesarean section. Newborns vaginally delivered.
| Sample_molecule_ch1 | other
| Sample_extract_protocol_ch1 | Total RNA by Trizol extraction according to the manufacturer's instructions. Polysomal RNA from sucrose density gradient fractions where n> 7 ribosomes/mRNA, extracted by guanidine-HCl, phenol/CHCl3 extracted, and ethanol precipitated. All further purified with Qiagen RNeasy according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 5 ug RNA using Enzo IVT kit for in vitro transcription
| Sample_hyb_protocol | 15 ug of labeled cRNA were used for hybridization to Affymetrix RAE2302 chips using protocol EukGE-WS2v4
| Sample_scan_protocol | Scanned using Affymetrix GeneChip Scanner 3000
| Sample_data_processing | scaling target signal 150 using GCOS 1.4
| Sample_platform_id | GPL1355
| Sample_contact_name | Gail,,Otulakowski
| Sample_contact_department | Physiology & Experimental Medicine
| Sample_contact_institute | Hospital for Sick Children Research Institute
| Sample_contact_address | 555 University Ave
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM306nnn/GSM306047/suppl/GSM306047.CEL.gz
| Sample_series_id | GSE12153
| Sample_data_row_count | 31099
| |
|
GSM306048 | GPL1355 |
|
FD19 lung, polysomal RNA, biological replicate 3
|
RNA from large polysomes from FD19 rat lung
|
Strain: Wistar
|
polysome-associated RNA from canalicular lung
|
Sample_geo_accession | GSM306048
| Sample_status | Public on Jul 07 2009
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 07 2009
| Sample_type | other
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | lungs excised and snap-frozen in liquid nitrogen
| Sample_growth_protocol_ch1 | fetuses and newborn rats from timed pregnant gestation. Fetuses delivered by Cesarean section. Newborns vaginally delivered.
| Sample_molecule_ch1 | other
| Sample_extract_protocol_ch1 | Total RNA by Trizol extraction according to the manufacturer's instructions. Polysomal RNA from sucrose density gradient fractions where n> 7 ribosomes/mRNA, extracted by guanidine-HCl, phenol/CHCl3 extracted, and ethanol precipitated. All further purified with Qiagen RNeasy according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 5 ug RNA using Enzo IVT kit for in vitro transcription
| Sample_hyb_protocol | 15 ug of labeled cRNA were used for hybridization to Affymetrix RAE2302 chips using protocol EukGE-WS2v4
| Sample_scan_protocol | Scanned using Affymetrix GeneChip Scanner 3000
| Sample_data_processing | scaling target signal 150 using GCOS 1.4
| Sample_platform_id | GPL1355
| Sample_contact_name | Gail,,Otulakowski
| Sample_contact_department | Physiology & Experimental Medicine
| Sample_contact_institute | Hospital for Sick Children Research Institute
| Sample_contact_address | 555 University Ave
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM306nnn/GSM306048/suppl/GSM306048.CEL.gz
| Sample_series_id | GSE12153
| Sample_data_row_count | 31099
| |
|
GSM306049 | GPL1355 |
|
FD22 lung, total RNA, biological replicate 1
|
total RNA from FD22 rat lung
|
Strain: Wistar
|
steady-state expression data from prenatal saccular lung
|
Sample_geo_accession | GSM306049
| Sample_status | Public on Jul 07 2009
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 07 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | lungs excised and snap-frozen in liquid nitrogen
| Sample_growth_protocol_ch1 | fetuses and newborn rats from timed pregnant gestation. Fetuses delivered by Cesarean section. Newborns vaginally delivered.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA by Trizol extraction according to the manufacturer's instructions. Polysomal RNA from sucrose density gradient fractions where n> 7 ribosomes/mRNA, extracted by guanidine-HCl, phenol/CHCl3 extracted, and ethanol precipitated. All further purified with Qiagen RNeasy according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 5 ug RNA using Enzo IVT kit for in vitro transcription
| Sample_hyb_protocol | 15 ug of labeled cRNA were used for hybridization to Affymetrix RAE2302 chips using protocol EukGE-WS2v4
| Sample_scan_protocol | Scanned using Affymetrix GeneChip Scanner 3000
| Sample_data_processing | scaling target signal 150 using GCOS 1.4
| Sample_platform_id | GPL1355
| Sample_contact_name | Gail,,Otulakowski
| Sample_contact_department | Physiology & Experimental Medicine
| Sample_contact_institute | Hospital for Sick Children Research Institute
| Sample_contact_address | 555 University Ave
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM306nnn/GSM306049/suppl/GSM306049.CEL.gz
| Sample_series_id | GSE12153
| Sample_data_row_count | 31099
| |
|
GSM306050 | GPL1355 |
|
FD22 lung, total RNA, biological replicate 2
|
total RNA from FD22 rat lung
|
Strain: Wistar
|
steady-state expression data from prenatal saccular lung
|
Sample_geo_accession | GSM306050
| Sample_status | Public on Jul 07 2009
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 07 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | lungs excised and snap-frozen in liquid nitrogen
| Sample_growth_protocol_ch1 | fetuses and newborn rats from timed pregnant gestation. Fetuses delivered by Cesarean section. Newborns vaginally delivered.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA by Trizol extraction according to the manufacturer's instructions. Polysomal RNA from sucrose density gradient fractions where n> 7 ribosomes/mRNA, extracted by guanidine-HCl, phenol/CHCl3 extracted, and ethanol precipitated. All further purified with Qiagen RNeasy according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 5 ug RNA using Enzo IVT kit for in vitro transcription
| Sample_hyb_protocol | 15 ug of labeled cRNA were used for hybridization to Affymetrix RAE2302 chips using protocol EukGE-WS2v4
| Sample_scan_protocol | Scanned using Affymetrix GeneChip Scanner 3000
| Sample_data_processing | scaling target signal 150 using GCOS 1.4
| Sample_platform_id | GPL1355
| Sample_contact_name | Gail,,Otulakowski
| Sample_contact_department | Physiology & Experimental Medicine
| Sample_contact_institute | Hospital for Sick Children Research Institute
| Sample_contact_address | 555 University Ave
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM306nnn/GSM306050/suppl/GSM306050.CEL.gz
| Sample_series_id | GSE12153
| Sample_data_row_count | 31099
| |
|
GSM306051 | GPL1355 |
|
FD22 lung, total RNA, biological replicate 3
|
total RNA from FD22 rat lung
|
Strain: Wistar
|
steady-state expression data from prenatal saccular lung
|
Sample_geo_accession | GSM306051
| Sample_status | Public on Jul 07 2009
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 07 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | lungs excised and snap-frozen in liquid nitrogen
| Sample_growth_protocol_ch1 | fetuses and newborn rats from timed pregnant gestation. Fetuses delivered by Cesarean section. Newborns vaginally delivered.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA by Trizol extraction according to the manufacturer's instructions. Polysomal RNA from sucrose density gradient fractions where n> 7 ribosomes/mRNA, extracted by guanidine-HCl, phenol/CHCl3 extracted, and ethanol precipitated. All further purified with Qiagen RNeasy according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 5 ug RNA using Enzo IVT kit for in vitro transcription
| Sample_hyb_protocol | 15 ug of labeled cRNA were used for hybridization to Affymetrix RAE2302 chips using protocol EukGE-WS2v4
| Sample_scan_protocol | Scanned using Affymetrix GeneChip Scanner 3000
| Sample_data_processing | scaling target signal 150 using GCOS 1.4
| Sample_platform_id | GPL1355
| Sample_contact_name | Gail,,Otulakowski
| Sample_contact_department | Physiology & Experimental Medicine
| Sample_contact_institute | Hospital for Sick Children Research Institute
| Sample_contact_address | 555 University Ave
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM306nnn/GSM306051/suppl/GSM306051.CEL.gz
| Sample_series_id | GSE12153
| Sample_data_row_count | 31099
| |
|
GSM306052 | GPL1355 |
|
FD22 lung, polysomal RNA, biological replicate 1
|
RNA from large polysomes from FD22 rat lung
|
Strain: Wistar
|
polysome-associated RNA from prenatal saccular lung
|
Sample_geo_accession | GSM306052
| Sample_status | Public on Jul 07 2009
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 07 2009
| Sample_type | other
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | lungs excised and snap-frozen in liquid nitrogen
| Sample_growth_protocol_ch1 | fetuses and newborn rats from timed pregnant gestation. Fetuses delivered by Cesarean section. Newborns vaginally delivered.
| Sample_molecule_ch1 | other
| Sample_extract_protocol_ch1 | Total RNA by Trizol extraction according to the manufacturer's instructions. Polysomal RNA from sucrose density gradient fractions where n> 7 ribosomes/mRNA, extracted by guanidine-HCl, phenol/CHCl3 extracted, and ethanol precipitated. All further purified with Qiagen RNeasy according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 5 ug RNA using Enzo IVT kit for in vitro transcription
| Sample_hyb_protocol | 15 ug of labeled cRNA were used for hybridization to Affymetrix RAE2302 chips using protocol EukGE-WS2v4
| Sample_scan_protocol | Scanned using Affymetrix GeneChip Scanner 3000
| Sample_data_processing | scaling target signal 150 using GCOS 1.4
| Sample_platform_id | GPL1355
| Sample_contact_name | Gail,,Otulakowski
| Sample_contact_department | Physiology & Experimental Medicine
| Sample_contact_institute | Hospital for Sick Children Research Institute
| Sample_contact_address | 555 University Ave
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM306nnn/GSM306052/suppl/GSM306052.CEL.gz
| Sample_series_id | GSE12153
| Sample_data_row_count | 31099
| |
|
GSM306053 | GPL1355 |
|
FD22 lung, polysomal RNA, biological replicate 2
|
RNA from large polysomes from FD22 rat lung
|
Strain: Wistar
|
polysome-associated RNA from prenatal saccular lung
|
Sample_geo_accession | GSM306053
| Sample_status | Public on Jul 07 2009
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 07 2009
| Sample_type | other
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | lungs excised and snap-frozen in liquid nitrogen
| Sample_growth_protocol_ch1 | fetuses and newborn rats from timed pregnant gestation. Fetuses delivered by Cesarean section. Newborns vaginally delivered.
| Sample_molecule_ch1 | other
| Sample_extract_protocol_ch1 | Total RNA by Trizol extraction according to the manufacturer's instructions. Polysomal RNA from sucrose density gradient fractions where n> 7 ribosomes/mRNA, extracted by guanidine-HCl, phenol/CHCl3 extracted, and ethanol precipitated. All further purified with Qiagen RNeasy according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 5 ug RNA using Enzo IVT kit for in vitro transcription
| Sample_hyb_protocol | 15 ug of labeled cRNA were used for hybridization to Affymetrix RAE2302 chips using protocol EukGE-WS2v4
| Sample_scan_protocol | Scanned using Affymetrix GeneChip Scanner 3000
| Sample_data_processing | scaling target signal 150 using GCOS 1.4
| Sample_platform_id | GPL1355
| Sample_contact_name | Gail,,Otulakowski
| Sample_contact_department | Physiology & Experimental Medicine
| Sample_contact_institute | Hospital for Sick Children Research Institute
| Sample_contact_address | 555 University Ave
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM306nnn/GSM306053/suppl/GSM306053.CEL.gz
| Sample_series_id | GSE12153
| Sample_data_row_count | 31099
| |
|
GSM306054 | GPL1355 |
|
FD22 lung, polysomal RNA, biological replicate 3
|
RNA from large polysomes from FD22 rat lung
|
Strain: Wistar
|
polysome-associated RNA from prenatal saccular lung
|
Sample_geo_accession | GSM306054
| Sample_status | Public on Jul 07 2009
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 07 2009
| Sample_type | other
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | lungs excised and snap-frozen in liquid nitrogen
| Sample_growth_protocol_ch1 | fetuses and newborn rats from timed pregnant gestation. Fetuses delivered by Cesarean section. Newborns vaginally delivered.
| Sample_molecule_ch1 | other
| Sample_extract_protocol_ch1 | Total RNA by Trizol extraction according to the manufacturer's instructions. Polysomal RNA from sucrose density gradient fractions where n> 7 ribosomes/mRNA, extracted by guanidine-HCl, phenol/CHCl3 extracted, and ethanol precipitated. All further purified with Qiagen RNeasy according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 5 ug RNA using Enzo IVT kit for in vitro transcription
| Sample_hyb_protocol | 15 ug of labeled cRNA were used for hybridization to Affymetrix RAE2302 chips using protocol EukGE-WS2v4
| Sample_scan_protocol | Scanned using Affymetrix GeneChip Scanner 3000
| Sample_data_processing | scaling target signal 150 using GCOS 1.4
| Sample_platform_id | GPL1355
| Sample_contact_name | Gail,,Otulakowski
| Sample_contact_department | Physiology & Experimental Medicine
| Sample_contact_institute | Hospital for Sick Children Research Institute
| Sample_contact_address | 555 University Ave
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM306nnn/GSM306054/suppl/GSM306054.CEL.gz
| Sample_series_id | GSE12153
| Sample_data_row_count | 31099
| |
|
GSM306055 | GPL1355 |
|
P1 lung, total RNA, biological replicate 1
|
total RNA from P1 rat lung
|
Strain: Wistar
|
steady-state expression data from postnatal saccular lung
|
Sample_geo_accession | GSM306055
| Sample_status | Public on Jul 07 2009
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 07 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | lungs excised and snap-frozen in liquid nitrogen
| Sample_growth_protocol_ch1 | fetuses and newborn rats from timed pregnant gestation. Fetuses delivered by Cesarean section. Newborns vaginally delivered.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA by Trizol extraction according to the manufacturer's instructions. Polysomal RNA from sucrose density gradient fractions where n> 7 ribosomes/mRNA, extracted by guanidine-HCl, phenol/CHCl3 extracted, and ethanol precipitated. All further purified with Qiagen RNeasy according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 5 ug RNA using Enzo IVT kit for in vitro transcription
| Sample_hyb_protocol | 15 ug of labeled cRNA were used for hybridization to Affymetrix RAE2302 chips using protocol EukGE-WS2v4
| Sample_scan_protocol | Scanned using Affymetrix GeneChip Scanner 3000
| Sample_data_processing | scaling target signal 150 using GCOS 1.4
| Sample_platform_id | GPL1355
| Sample_contact_name | Gail,,Otulakowski
| Sample_contact_department | Physiology & Experimental Medicine
| Sample_contact_institute | Hospital for Sick Children Research Institute
| Sample_contact_address | 555 University Ave
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM306nnn/GSM306055/suppl/GSM306055.CEL.gz
| Sample_series_id | GSE12153
| Sample_data_row_count | 31099
| |
|
GSM306056 | GPL1355 |
|
P1 lung, total RNA, biological replicate 2
|
total RNA from P1 rat lung
|
Strain: Wistar
|
steady-state expression data from postnatal saccular lung
|
Sample_geo_accession | GSM306056
| Sample_status | Public on Jul 07 2009
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 07 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | lungs excised and snap-frozen in liquid nitrogen
| Sample_growth_protocol_ch1 | fetuses and newborn rats from timed pregnant gestation. Fetuses delivered by Cesarean section. Newborns vaginally delivered.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA by Trizol extraction according to the manufacturer's instructions. Polysomal RNA from sucrose density gradient fractions where n> 7 ribosomes/mRNA, extracted by guanidine-HCl, phenol/CHCl3 extracted, and ethanol precipitated. All further purified with Qiagen RNeasy according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 5 ug RNA using Enzo IVT kit for in vitro transcription
| Sample_hyb_protocol | 15 ug of labeled cRNA were used for hybridization to Affymetrix RAE2302 chips using protocol EukGE-WS2v4
| Sample_scan_protocol | Scanned using Affymetrix GeneChip Scanner 3000
| Sample_data_processing | scaling target signal 150 using GCOS 1.4
| Sample_platform_id | GPL1355
| Sample_contact_name | Gail,,Otulakowski
| Sample_contact_department | Physiology & Experimental Medicine
| Sample_contact_institute | Hospital for Sick Children Research Institute
| Sample_contact_address | 555 University Ave
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM306nnn/GSM306056/suppl/GSM306056.CEL.gz
| Sample_series_id | GSE12153
| Sample_data_row_count | 31099
| |
|
GSM306057 | GPL1355 |
|
P1 lung, total RNA, biological replicate 3
|
total RNA from P1 rat lung
|
Strain: Wistar
|
steady-state expression data from postnatal saccular lung
|
Sample_geo_accession | GSM306057
| Sample_status | Public on Jul 07 2009
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 07 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | lungs excised and snap-frozen in liquid nitrogen
| Sample_growth_protocol_ch1 | fetuses and newborn rats from timed pregnant gestation. Fetuses delivered by Cesarean section. Newborns vaginally delivered.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA by Trizol extraction according to the manufacturer's instructions. Polysomal RNA from sucrose density gradient fractions where n> 7 ribosomes/mRNA, extracted by guanidine-HCl, phenol/CHCl3 extracted, and ethanol precipitated. All further purified with Qiagen RNeasy according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 5 ug RNA using Enzo IVT kit for in vitro transcription
| Sample_hyb_protocol | 15 ug of labeled cRNA were used for hybridization to Affymetrix RAE2302 chips using protocol EukGE-WS2v4
| Sample_scan_protocol | Scanned using Affymetrix GeneChip Scanner 3000
| Sample_data_processing | scaling target signal 150 using GCOS 1.4
| Sample_platform_id | GPL1355
| Sample_contact_name | Gail,,Otulakowski
| Sample_contact_department | Physiology & Experimental Medicine
| Sample_contact_institute | Hospital for Sick Children Research Institute
| Sample_contact_address | 555 University Ave
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM306nnn/GSM306057/suppl/GSM306057.CEL.gz
| Sample_series_id | GSE12153
| Sample_data_row_count | 31099
| |
|
GSM306058 | GPL1355 |
|
P1 lung, polysomal RNA, biological replicate 1
|
RNA from large polysomes from P1 rat lung
|
Strain: Wistar
|
polysome-associated RNA from postnatal saccular lung
|
Sample_geo_accession | GSM306058
| Sample_status | Public on Jul 07 2009
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 07 2009
| Sample_type | other
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | lungs excised and snap-frozen in liquid nitrogen
| Sample_growth_protocol_ch1 | fetuses and newborn rats from timed pregnant gestation. Fetuses delivered by Cesarean section. Newborns vaginally delivered.
| Sample_molecule_ch1 | other
| Sample_extract_protocol_ch1 | Total RNA by Trizol extraction according to the manufacturer's instructions. Polysomal RNA from sucrose density gradient fractions where n> 7 ribosomes/mRNA, extracted by guanidine-HCl, phenol/CHCl3 extracted, and ethanol precipitated. All further purified with Qiagen RNeasy according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 5 ug RNA using Enzo IVT kit for in vitro transcription
| Sample_hyb_protocol | 15 ug of labeled cRNA were used for hybridization to Affymetrix RAE2302 chips using protocol EukGE-WS2v4
| Sample_scan_protocol | Scanned using Affymetrix GeneChip Scanner 3000
| Sample_data_processing | scaling target signal 150 using GCOS 1.4
| Sample_platform_id | GPL1355
| Sample_contact_name | Gail,,Otulakowski
| Sample_contact_department | Physiology & Experimental Medicine
| Sample_contact_institute | Hospital for Sick Children Research Institute
| Sample_contact_address | 555 University Ave
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM306nnn/GSM306058/suppl/GSM306058.CEL.gz
| Sample_series_id | GSE12153
| Sample_data_row_count | 31099
| |
|
GSM306059 | GPL1355 |
|
P1 lung, polysomal RNA, biological replicate 2
|
RNA from large polysomes from P1 rat lung
|
Strain: Wistar
|
polysome-associated RNA from postnatal saccular lung
|
Sample_geo_accession | GSM306059
| Sample_status | Public on Jul 07 2009
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 07 2009
| Sample_type | other
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | lungs excised and snap-frozen in liquid nitrogen
| Sample_growth_protocol_ch1 | fetuses and newborn rats from timed pregnant gestation. Fetuses delivered by Cesarean section. Newborns vaginally delivered.
| Sample_molecule_ch1 | other
| Sample_extract_protocol_ch1 | Total RNA by Trizol extraction according to the manufacturer's instructions. Polysomal RNA from sucrose density gradient fractions where n> 7 ribosomes/mRNA, extracted by guanidine-HCl, phenol/CHCl3 extracted, and ethanol precipitated. All further purified with Qiagen RNeasy according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 5 ug RNA using Enzo IVT kit for in vitro transcription
| Sample_hyb_protocol | 15 ug of labeled cRNA were used for hybridization to Affymetrix RAE2302 chips using protocol EukGE-WS2v4
| Sample_scan_protocol | Scanned using Affymetrix GeneChip Scanner 3000
| Sample_data_processing | scaling target signal 150 using GCOS 1.4
| Sample_platform_id | GPL1355
| Sample_contact_name | Gail,,Otulakowski
| Sample_contact_department | Physiology & Experimental Medicine
| Sample_contact_institute | Hospital for Sick Children Research Institute
| Sample_contact_address | 555 University Ave
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM306nnn/GSM306059/suppl/GSM306059.CEL.gz
| Sample_series_id | GSE12153
| Sample_data_row_count | 31099
| |
|
GSM306060 | GPL1355 |
|
P1 lung, polysomal RNA, biological replicate 3
|
RNA from large polysomes from P1 rat lung
|
Strain: Wistar
|
polysome-associated RNA from postnatal saccular lung
|
Sample_geo_accession | GSM306060
| Sample_status | Public on Jul 07 2009
| Sample_submission_date | Jul 17 2008
| Sample_last_update_date | Jul 07 2009
| Sample_type | other
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | lungs excised and snap-frozen in liquid nitrogen
| Sample_growth_protocol_ch1 | fetuses and newborn rats from timed pregnant gestation. Fetuses delivered by Cesarean section. Newborns vaginally delivered.
| Sample_molecule_ch1 | other
| Sample_extract_protocol_ch1 | Total RNA by Trizol extraction according to the manufacturer's instructions. Polysomal RNA from sucrose density gradient fractions where n> 7 ribosomes/mRNA, extracted by guanidine-HCl, phenol/CHCl3 extracted, and ethanol precipitated. All further purified with Qiagen RNeasy according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 5 ug RNA using Enzo IVT kit for in vitro transcription
| Sample_hyb_protocol | 15 ug of labeled cRNA were used for hybridization to Affymetrix RAE2302 chips using protocol EukGE-WS2v4
| Sample_scan_protocol | Scanned using Affymetrix GeneChip Scanner 3000
| Sample_data_processing | scaling target signal 150 using GCOS 1.4
| Sample_platform_id | GPL1355
| Sample_contact_name | Gail,,Otulakowski
| Sample_contact_department | Physiology & Experimental Medicine
| Sample_contact_institute | Hospital for Sick Children Research Institute
| Sample_contact_address | 555 University Ave
| Sample_contact_city | Toronto
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | M5G 1X8
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM306nnn/GSM306060/suppl/GSM306060.CEL.gz
| Sample_series_id | GSE12153
| Sample_data_row_count | 31099
| |
|
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Select GSMs and click on "Add groups" |
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