Search results for the GEO ID: GSE12205 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM307029 | GPL570 |
|
HLF 1-1 (No)
|
Human lung fibroblasts
|
Normal diploid human lung fibroblast cell line, LL24, was originally isolated from normal autopsy tissue of an 11-year-old male.
|
no additional information
|
Sample_geo_accession | GSM307029
| Sample_status | Public on Dec 02 2008
| Sample_submission_date | Jul 22 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | culture medium for 24hr
| Sample_growth_protocol_ch1 | Cells were seeded at 2 million/150mm dish and incubated with Ham's F-12 nutrient mixture, supplemented with 15% FBS, 50 units/ml penicilin and 50 ug/ml streptomycin for 24h, total RNA was isolated after incubation with F12 medium for 24hr.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Following 24hr treatment with culture medium, RNA was isolated using RNA-Bee (Tel-Test, Friendswood, TX) followed by a secondary purification on Qiagen RNAeasy Mini columns (Qiagen, Valencia, CA). RNA quality was assessed by Agilent bioanalyzer and RNA Integrity Numbers (RIN) for samples were >9.9.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA (10 ug) was labeled by One-Cycle Target Labeling Kit as specified by Affymetrix.
| Sample_hyb_protocol | Hybridization, washing and staining were carried out according to the Affymetrix’s instructions.
| Sample_scan_protocol | GeneChip Human Genome U133 Plus 2.0 Arrays were scanned with an Agilent laser scanner.
| Sample_data_processing | Default normalization steps recommended by GeneSpring software were used:
| Sample_data_processing | (1) data transformation: set measurements less than 0.01 to 0.01
| Sample_data_processing | (2) per chip: normalize to 50th percentile
| Sample_data_processing | (3) per gene: normalize to median
| Sample_platform_id | GPL570
| Sample_contact_name | DONG-SOON,,BAE
| Sample_contact_email | phmdxb@gwumc.edu
| Sample_contact_phone | 202-994-0964
| Sample_contact_fax | 202-994-2870
| Sample_contact_department | Pharmacology & Physiology
| Sample_contact_institute | George Washinton Uni.
| Sample_contact_address | 2300 I Street, NW
| Sample_contact_city | Washington
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20037
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307029/suppl/GSM307029.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307029/suppl/GSM307029.CHP.gz
| Sample_series_id | GSE12205
| Sample_data_row_count | 54675
| |
|
GSM307033 | GPL570 |
|
HLF 1-2 (No)
|
Human lung fibroblats
|
Normal diploid human lung fibroblast cell line, LL24, was originally isolated from normal autopsy tissue of an 11-year-old male.
|
no additional information
|
Sample_geo_accession | GSM307033
| Sample_status | Public on Dec 02 2008
| Sample_submission_date | Jul 22 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | culture medium for 24hr
| Sample_growth_protocol_ch1 | Cells were seeded at 2 million/150mm dish and incubated with Ham's F-12 nutrient mixture, supplemented with 15% FBS, 50 units/ml penicilin and 50 ug/ml streptomycin for 24h, total RNA was isolated after incubation with F12 medium for 24hr.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Following 24hr treatment with culture medium, RNA was isolated using RNA-Bee (Tel-Test, Friendswood, TX) followed by a secondary purification on Qiagen RNAeasy Mini columns (Qiagen, Valencia, CA). RNA quality was assessed by Agilent bioanalyzer and RNA Integrity Numbers (RIN) for samples were >9.9.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA (10 ug) was labeled by One-Cycle Target Labeling Kit as specified by Affymetrix.
| Sample_hyb_protocol | Hybridization, washing and staining were carried out according to the Affymetrix’s instructions.
| Sample_scan_protocol | GeneChip Human Genome U133 Plus 2.0 Arrays were scanned with an Agilent laser scanner.
| Sample_data_processing | Default normalization steps recommended by GeneSpring software were used:
| Sample_data_processing | (1) data transformation: set measurements less than 0.01 to 0.01
| Sample_data_processing | (2) per chip: normalize to 50th percentile
| Sample_data_processing | (3) per gene: normalize to median
| Sample_platform_id | GPL570
| Sample_contact_name | DONG-SOON,,BAE
| Sample_contact_email | phmdxb@gwumc.edu
| Sample_contact_phone | 202-994-0964
| Sample_contact_fax | 202-994-2870
| Sample_contact_department | Pharmacology & Physiology
| Sample_contact_institute | George Washinton Uni.
| Sample_contact_address | 2300 I Street, NW
| Sample_contact_city | Washington
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20037
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307033/suppl/GSM307033.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307033/suppl/GSM307033.CHP.gz
| Sample_series_id | GSE12205
| Sample_data_row_count | 54675
| |
|
GSM307052 | GPL570 |
|
HLF 1-3 (No)
|
Human lung fibroblasts
|
Normal diploid human lung fibroblast cell line, LL24, was originally isolated from normal autopsy tissue of an 11-year-old male.
|
no additional information
|
Sample_geo_accession | GSM307052
| Sample_status | Public on Dec 02 2008
| Sample_submission_date | Jul 22 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | culture medium for 24hr
| Sample_growth_protocol_ch1 | Cells were seeded at 2 million/150mm dish and incubated with Ham's F-12 nutrient mixture, supplemented with 15% FBS, 50 units/ml penicilin and 50 ug/ml streptomycin for 24h, total RNA was isolated after incubation with F12 medium for 24hr.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Following 24hr treatment with culture medium, RNA was isolated using RNA-Bee (Tel-Test, Friendswood, TX) followed by a secondary purification on Qiagen RNAeasy Mini columns (Qiagen, Valencia, CA). RNA quality was assessed by Agilent bioanalyzer and RNA Integrity Numbers (RIN) for samples were >9.9.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA (10 ug) was labeled by One-Cycle Target Labeling Kit as specified by Affymetrix.
| Sample_hyb_protocol | Hybridization, washing and staining were carried out according to the Affymetrix’s instructions.
| Sample_scan_protocol | GeneChip Human Genome U133 Plus 2.0 Arrays were scanned with an Agilent laser scanner.
| Sample_data_processing | Default normalization steps recommended by GeneSpring software were used:
| Sample_data_processing | (1) data transformation: set measurements less than 0.01 to 0.01
| Sample_data_processing | (2) per chip: normalize to 50th percentile
| Sample_data_processing | (3) per gene: normalize to median
| Sample_platform_id | GPL570
| Sample_contact_name | DONG-SOON,,BAE
| Sample_contact_email | phmdxb@gwumc.edu
| Sample_contact_phone | 202-994-0964
| Sample_contact_fax | 202-994-2870
| Sample_contact_department | Pharmacology & Physiology
| Sample_contact_institute | George Washinton Uni.
| Sample_contact_address | 2300 I Street, NW
| Sample_contact_city | Washington
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20037
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307052/suppl/GSM307052.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307052/suppl/GSM307052.CHP.gz
| Sample_series_id | GSE12205
| Sample_data_row_count | 54675
| |
|
GSM307054 | GPL570 |
|
HLF 1-4 (No)
|
Human lung fibroblasts
|
Normal diploid human lung fibroblast cell line, LL24, was originally isolated from normal autopsy tissue of an 11-year-old male.
|
no additional information
|
Sample_geo_accession | GSM307054
| Sample_status | Public on Dec 02 2008
| Sample_submission_date | Jul 22 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | culture medium for 24hr
| Sample_growth_protocol_ch1 | Cells were seeded at 2 million/150mm dish and incubated with Ham's F-12 nutrient mixture, supplemented with 15% FBS, 50 units/ml penicilin and 50 ug/ml streptomycin for 24h, total RNA was isolated after incubation with F12 medium for 24hr.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Following 24hr treatment with culture medium, RNA was isolated using RNA-Bee (Tel-Test, Friendswood, TX) followed by a secondary purification on Qiagen RNAeasy Mini columns (Qiagen, Valencia, CA). RNA quality was assessed by Agilent bioanalyzer and RNA Integrity Numbers (RIN) for samples were >9.9.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA (10 ug) was labeled by One-Cycle Target Labeling Kit as specified by Affymetrix.
| Sample_hyb_protocol | Hybridization, washing and staining were carried out according to the Affymetrix’s instructions.
| Sample_scan_protocol | GeneChip Human Genome U133 Plus 2.0 Arrays were scanned with an Agilent laser scanner.
| Sample_data_processing | Default normalization steps recommended by GeneSpring software were used:
| Sample_data_processing | (1) data transformation: set measurements less than 0.01 to 0.01
| Sample_data_processing | (2) per chip: normalize to 50th percentile
| Sample_data_processing | (3) per gene: normalize to median
| Sample_platform_id | GPL570
| Sample_contact_name | DONG-SOON,,BAE
| Sample_contact_email | phmdxb@gwumc.edu
| Sample_contact_phone | 202-994-0964
| Sample_contact_fax | 202-994-2870
| Sample_contact_department | Pharmacology & Physiology
| Sample_contact_institute | George Washinton Uni.
| Sample_contact_address | 2300 I Street, NW
| Sample_contact_city | Washington
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20037
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307054/suppl/GSM307054.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307054/suppl/GSM307054.CHP.gz
| Sample_series_id | GSE12205
| Sample_data_row_count | 54675
| |
|
GSM307059 | GPL570 |
|
HLF 3-1 (Cr)
|
Human lung fibroblasts
|
Normal diploid human lung fibroblast cell line, LL24, was originally isolated from normal autopsy tissue of an 11-year-old male.
|
no additional information
|
Sample_geo_accession | GSM307059
| Sample_status | Public on Dec 02 2008
| Sample_submission_date | Jul 22 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | Sodium chromate [Cr(VI)] for 24hr
| Sample_growth_protocol_ch1 | Cells were seeded at 2 million/150mm dish and incubated with Ham's F-12 nutrient mixture, supplemented with 15% FBS, 50 units/ml penicilin and 50 ug/ml streptomycin for 24h, total RNA was isolated after incubation with 1uM Cr(VI) for 24hr.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Following 24hr treatment with 1uM Cr(VI), RNA was isolated using RNA-Bee (Tel-Test, Friendswood, TX) followed by a secondary purification on Qiagen RNAeasy Mini columns (Qiagen, Valencia, CA). RNA quality was assessed by Agilent bioanalyzer and RNA Integrity Numbers (RIN) for samples were >9.9.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA (10 ug) was labeled by One-Cycle Target Labeling Kit as specified by Affymetrix.
| Sample_hyb_protocol | Hybridization, washing and staining were carried out according to the Affymetrix’s instructions.
| Sample_scan_protocol | GeneChip Human Genome U133 Plus 2.0 Arrays were scanned with an Agilent laser scanner.
| Sample_data_processing | Default normalization steps recommended by GeneSpring software were used:
| Sample_data_processing | (1) data transformation: set measurements less than 0.01 to 0.01
| Sample_data_processing | (2) per chip: normalize to 50th percentile
| Sample_data_processing | (3) per gene: normalize to median
| Sample_platform_id | GPL570
| Sample_contact_name | DONG-SOON,,BAE
| Sample_contact_email | phmdxb@gwumc.edu
| Sample_contact_phone | 202-994-0964
| Sample_contact_fax | 202-994-2870
| Sample_contact_department | Pharmacology & Physiology
| Sample_contact_institute | George Washinton Uni.
| Sample_contact_address | 2300 I Street, NW
| Sample_contact_city | Washington
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20037
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307059/suppl/GSM307059.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307059/suppl/GSM307059.CHP.gz
| Sample_series_id | GSE12205
| Sample_data_row_count | 54675
| |
|
GSM307060 | GPL570 |
|
HLF 3-2 (Cr)
|
Human lung fibroblasts
|
Normal diploid human lung fibroblast cell line, LL24, was originally isolated from normal autopsy tissue of an 11-year-old male.
|
no additional information
|
Sample_geo_accession | GSM307060
| Sample_status | Public on Dec 02 2008
| Sample_submission_date | Jul 22 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | Sodium chromate [Cr(VI)] for 24hr
| Sample_growth_protocol_ch1 | Cells were seeded at 2 million/150mm dish and incubated with Ham's F-12 nutrient mixture, supplemented with 15% FBS, 50 units/ml penicilin and 50 ug/ml streptomycin for 24h, total RNA was isolated after incubation with 1uM Cr(VI) for 24hr.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Following 24hr treatment with 1uM Cr(VI), RNA was isolated using RNA-Bee (Tel-Test, Friendswood, TX) followed by a secondary purification on Qiagen RNAeasy Mini columns (Qiagen, Valencia, CA). RNA quality was assessed by Agilent bioanalyzer and RNA Integrity Numbers (RIN) for samples were >9.9.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA (10 ug) was labeled by One-Cycle Target Labeling Kit as specified by Affymetrix.
| Sample_hyb_protocol | Hybridization, washing and staining were carried out according to the Affymetrix’s instructions.
| Sample_scan_protocol | GeneChip Human Genome U133 Plus 2.0 Arrays were scanned with an Agilent laser scanner.
| Sample_data_processing | Default normalization steps recommended by GeneSpring software were used:
| Sample_data_processing | (1) data transformation: set measurements less than 0.01 to 0.01
| Sample_data_processing | (2) per chip: normalize to 50th percentile
| Sample_data_processing | (3) per gene: normalize to median
| Sample_platform_id | GPL570
| Sample_contact_name | DONG-SOON,,BAE
| Sample_contact_email | phmdxb@gwumc.edu
| Sample_contact_phone | 202-994-0964
| Sample_contact_fax | 202-994-2870
| Sample_contact_department | Pharmacology & Physiology
| Sample_contact_institute | George Washinton Uni.
| Sample_contact_address | 2300 I Street, NW
| Sample_contact_city | Washington
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20037
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307060/suppl/GSM307060.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307060/suppl/GSM307060.CHP.gz
| Sample_series_id | GSE12205
| Sample_data_row_count | 54675
| |
|
GSM307061 | GPL570 |
|
HLF 3-3 (Cr)
|
Human lung fibroblasts
|
Normal diploid human lung fibroblast cell line, LL24, was originally isolated from normal autopsy tissue of an 11-year-old male.
|
no additional information
|
Sample_geo_accession | GSM307061
| Sample_status | Public on Dec 02 2008
| Sample_submission_date | Jul 22 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | Sodium chromate [Cr(VI)] for 24hr
| Sample_growth_protocol_ch1 | Cells were seeded at 2 million/150mm dish and incubated with Ham's F-12 nutrient mixture, supplemented with 15% FBS, 50 units/ml penicilin and 50 ug/ml streptomycin for 24h, total RNA was isolated after incubation with 1uM Cr(VI) for 24hr.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Following 24hr treatment with 1uM Cr(VI), RNA was isolated using RNA-Bee (Tel-Test, Friendswood, TX) followed by a secondary purification on Qiagen RNAeasy Mini columns (Qiagen, Valencia, CA). RNA quality was assessed by Agilent bioanalyzer and RNA Integrity Numbers (RIN) for samples were >9.9.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA (10 ug) was labeled by One-Cycle Target Labeling Kit as specified by Affymetrix.
| Sample_hyb_protocol | Hybridization, washing and staining were carried out according to the Affymetrix’s instructions.
| Sample_scan_protocol | GeneChip Human Genome U133 Plus 2.0 Arrays were scanned with an Agilent laser scanner.
| Sample_data_processing | Default normalization steps recommended by GeneSpring software were used:
| Sample_data_processing | (1) data transformation: set measurements less than 0.01 to 0.01
| Sample_data_processing | (2) per chip: normalize to 50th percentile
| Sample_data_processing | (3) per gene: normalize to median
| Sample_platform_id | GPL570
| Sample_contact_name | DONG-SOON,,BAE
| Sample_contact_email | phmdxb@gwumc.edu
| Sample_contact_phone | 202-994-0964
| Sample_contact_fax | 202-994-2870
| Sample_contact_department | Pharmacology & Physiology
| Sample_contact_institute | George Washinton Uni.
| Sample_contact_address | 2300 I Street, NW
| Sample_contact_city | Washington
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20037
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307061/suppl/GSM307061.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307061/suppl/GSM307061.CHP.gz
| Sample_series_id | GSE12205
| Sample_data_row_count | 54675
| |
|
GSM307063 | GPL570 |
|
HLF 3-4 (Cr)
|
Human lung fibroblasts
|
Normal diploid human lung fibroblast cell line, LL24, was originally isolated from normal autopsy tissue of an 11-year-old male.
|
no additional information
|
Sample_geo_accession | GSM307063
| Sample_status | Public on Dec 02 2008
| Sample_submission_date | Jul 22 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | Sodium chromate [Cr(VI)] for 24hr
| Sample_growth_protocol_ch1 | Cells were seeded at 2 million/150mm dish and incubated with Ham's F-12 nutrient mixture, supplemented with 15% FBS, 50 units/ml penicilin and 50 ug/ml streptomycin for 24h, total RNA was isolated after incubation with 1uM Cr(VI) for 24hr.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Following 24hr treatment with 1uM Cr(VI), RNA was isolated using RNA-Bee (Tel-Test, Friendswood, TX) followed by a secondary purification on Qiagen RNAeasy Mini columns (Qiagen, Valencia, CA). RNA quality was assessed by Agilent bioanalyzer and RNA Integrity Numbers (RIN) for samples were >9.9.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA (10 ug) was labeled by One-Cycle Target Labeling Kit as specified by Affymetrix.
| Sample_hyb_protocol | Hybridization, washing and staining were carried out according to the Affymetrix’s instructions.
| Sample_scan_protocol | GeneChip Human Genome U133 Plus 2.0 Arrays were scanned with an Agilent laser scanner.
| Sample_data_processing | Default normalization steps recommended by GeneSpring software were used:
| Sample_data_processing | (1) data transformation: set measurements less than 0.01 to 0.01
| Sample_data_processing | (2) per chip: normalize to 50th percentile
| Sample_data_processing | (3) per gene: normalize to median
| Sample_platform_id | GPL570
| Sample_contact_name | DONG-SOON,,BAE
| Sample_contact_email | phmdxb@gwumc.edu
| Sample_contact_phone | 202-994-0964
| Sample_contact_fax | 202-994-2870
| Sample_contact_department | Pharmacology & Physiology
| Sample_contact_institute | George Washinton Uni.
| Sample_contact_address | 2300 I Street, NW
| Sample_contact_city | Washington
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20037
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307063/suppl/GSM307063.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307063/suppl/GSM307063.CHP.gz
| Sample_series_id | GSE12205
| Sample_data_row_count | 54675
| |
|
GSM307073 | GPL570 |
|
HLF 4-1 (SOV+Cr)
|
Human lung fibroblasts
|
Normal diploid human lung fibroblast cell line, LL24, was originally isolated from normal autopsy tissue of an 11-year-old male.
|
no additional information
|
Sample_geo_accession | GSM307073
| Sample_status | Public on Dec 02 2008
| Sample_submission_date | Jul 22 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | Sodium chromate [Cr(VI)] in the presence of SOV for 24hr
| Sample_growth_protocol_ch1 | Cells were seeded at 2 million/150mm dish and incubated with Ham's F-12 nutrient mixture, supplemented with 15% FBS, 50 units/ml penicilin and 50 ug/ml streptomycin for 24h, total RNA was isolated after incubation with 1uM Cr(VI) + 10uM SOV for 24hr. The SOV was added 30 min prior to Cr(VI) addition.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Following 24hr treatment with 1uM Cr(VI) + 10uM SOV, RNA was isolated using RNA-Bee (Tel-Test, Friendswood, TX) followed by a secondary purification on Qiagen RNAeasy Mini columns (Qiagen, Valencia, CA). RNA quality was assessed by Agilent bioanalyzer and RNA Integrity Numbers (RIN) for samples were >9.9.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA (10 ug) was labeled by One-Cycle Target Labeling Kit as specified by Affymetrix.
| Sample_hyb_protocol | Hybridization, washing and staining were carried out according to the Affymetrix’s instructions.
| Sample_scan_protocol | GeneChip Human Genome U133 Plus 2.0 Arrays were scanned with an Agilent laser scanner.
| Sample_data_processing | Default normalization steps recommended by GeneSpring software were used:
| Sample_data_processing | (1) data transformation: set measurements less than 0.01 to 0.01
| Sample_data_processing | (2) per chip: normalize to 50th percentile
| Sample_data_processing | (3) per gene: normalize to median
| Sample_platform_id | GPL570
| Sample_contact_name | DONG-SOON,,BAE
| Sample_contact_email | phmdxb@gwumc.edu
| Sample_contact_phone | 202-994-0964
| Sample_contact_fax | 202-994-2870
| Sample_contact_department | Pharmacology & Physiology
| Sample_contact_institute | George Washinton Uni.
| Sample_contact_address | 2300 I Street, NW
| Sample_contact_city | Washington
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20037
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307073/suppl/GSM307073.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307073/suppl/GSM307073.CHP.gz
| Sample_series_id | GSE12205
| Sample_data_row_count | 54675
| |
|
GSM307074 | GPL570 |
|
HLF 4-2 (SOV+Cr)
|
Human lung fibroblasts
|
Normal diploid human lung fibroblast cell line, LL24, was originally isolated from normal autopsy tissue of an 11-year-old male.
|
no additional information
|
Sample_geo_accession | GSM307074
| Sample_status | Public on Dec 02 2008
| Sample_submission_date | Jul 22 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | Sodium chromate [Cr(VI)] in the presence of SOV for 24hr
| Sample_growth_protocol_ch1 | Cells were seeded at 2 million/150mm dish and incubated with Ham's F-12 nutrient mixture, supplemented with 15% FBS, 50 units/ml penicilin and 50 ug/ml streptomycin for 24h, total RNA was isolated after incubation with 1uM Cr(VI) + 10uM SOV for 24hr. The SOV was added 30 min prior to Cr(VI) addition.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Following 24hr treatment with 1uM Cr(VI) + 10uM SOV, RNA was isolated using RNA-Bee (Tel-Test, Friendswood, TX) followed by a secondary purification on Qiagen RNAeasy Mini columns (Qiagen, Valencia, CA). RNA quality was assessed by Agilent bioanalyzer and RNA Integrity Numbers (RIN) for samples were >9.9.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA (10 ug) was labeled by One-Cycle Target Labeling Kit as specified by Affymetrix.
| Sample_hyb_protocol | Hybridization, washing and staining were carried out according to the Affymetrix’s instructions.
| Sample_scan_protocol | GeneChip Human Genome U133 Plus 2.0 Arrays were scanned with an Agilent laser scanner.
| Sample_data_processing | Default normalization steps recommended by GeneSpring software were used:
| Sample_data_processing | (1) data transformation: set measurements less than 0.01 to 0.01
| Sample_data_processing | (2) per chip: normalize to 50th percentile
| Sample_data_processing | (3) per gene: normalize to median
| Sample_platform_id | GPL570
| Sample_contact_name | DONG-SOON,,BAE
| Sample_contact_email | phmdxb@gwumc.edu
| Sample_contact_phone | 202-994-0964
| Sample_contact_fax | 202-994-2870
| Sample_contact_department | Pharmacology & Physiology
| Sample_contact_institute | George Washinton Uni.
| Sample_contact_address | 2300 I Street, NW
| Sample_contact_city | Washington
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20037
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307074/suppl/GSM307074.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307074/suppl/GSM307074.CHP.gz
| Sample_series_id | GSE12205
| Sample_data_row_count | 54675
| |
|
GSM307075 | GPL570 |
|
HLF 4-3 (SOV+Cr)
|
Human lung fibroblasts
|
Normal diploid human lung fibroblast cell line, LL24, was originally isolated from normal autopsy tissue of an 11-year-old male.
|
no additional information
|
Sample_geo_accession | GSM307075
| Sample_status | Public on Dec 02 2008
| Sample_submission_date | Jul 22 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | Sodium chromate [Cr(VI)] in the presence of SOV for 24hr
| Sample_growth_protocol_ch1 | Cells were seeded at 2 million/150mm dish and incubated with Ham's F-12 nutrient mixture, supplemented with 15% FBS, 50 units/ml penicilin and 50 ug/ml streptomycin for 24h, total RNA was isolated after incubation with 1uM Cr(VI) + 10uM SOV for 24hr. The SOV was added 30 min prior to Cr(VI) addition.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Following 24hr treatment with 1uM Cr(VI) + 10uM SOV, RNA was isolated using RNA-Bee (Tel-Test, Friendswood, TX) followed by a secondary purification on Qiagen RNAeasy Mini columns (Qiagen, Valencia, CA). RNA quality was assessed by Agilent bioanalyzer and RNA Integrity Numbers (RIN) for samples were >9.9.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA (10 ug) was labeled by One-Cycle Target Labeling Kit as specified by Affymetrix.
| Sample_hyb_protocol | Hybridization, washing and staining were carried out according to the Affymetrix’s instructions.
| Sample_scan_protocol | GeneChip Human Genome U133 Plus 2.0 Arrays were scanned with an Agilent laser scanner.
| Sample_data_processing | Default normalization steps recommended by GeneSpring software were used:
| Sample_data_processing | (1) data transformation: set measurements less than 0.01 to 0.01
| Sample_data_processing | (2) per chip: normalize to 50th percentile
| Sample_data_processing | (3) per gene: normalize to median
| Sample_platform_id | GPL570
| Sample_contact_name | DONG-SOON,,BAE
| Sample_contact_email | phmdxb@gwumc.edu
| Sample_contact_phone | 202-994-0964
| Sample_contact_fax | 202-994-2870
| Sample_contact_department | Pharmacology & Physiology
| Sample_contact_institute | George Washinton Uni.
| Sample_contact_address | 2300 I Street, NW
| Sample_contact_city | Washington
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20037
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307075/suppl/GSM307075.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307075/suppl/GSM307075.CHP.gz
| Sample_series_id | GSE12205
| Sample_data_row_count | 54675
| |
|
GSM307077 | GPL570 |
|
HLF 4-4 (SOV+Cr)
|
Human lung fibroblasts
|
Normal diploid human lung fibroblast cell line, LL24, was originally isolated from normal autopsy tissue of an 11-year-old male.
|
no additional information
|
Sample_geo_accession | GSM307077
| Sample_status | Public on Dec 02 2008
| Sample_submission_date | Jul 22 2008
| Sample_last_update_date | Dec 02 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | Sodium chromate [Cr(VI)] in the presence of SOV for 24hr
| Sample_growth_protocol_ch1 | Cells were seeded at 2 million/150mm dish and incubated with Ham's F-12 nutrient mixture, supplemented with 15% FBS, 50 units/ml penicilin and 50 ug/ml streptomycin for 24h, total RNA was isolated after incubation with 1uM Cr(VI) + 10uM SOV for 24hr. The SOV was added 30 min prior to Cr(VI) addition.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Following 24hr treatment with 1uM Cr(VI) + 10uM SOV, RNA was isolated using RNA-Bee (Tel-Test, Friendswood, TX) followed by a secondary purification on Qiagen RNAeasy Mini columns (Qiagen, Valencia, CA). RNA quality was assessed by Agilent bioanalyzer and RNA Integrity Numbers (RIN) for samples were >9.9.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA (10 ug) was labeled by One-Cycle Target Labeling Kit as specified by Affymetrix.
| Sample_hyb_protocol | Hybridization, washing and staining were carried out according to the Affymetrix’s instructions.
| Sample_scan_protocol | GeneChip Human Genome U133 Plus 2.0 Arrays were scanned with an Agilent laser scanner.
| Sample_data_processing | Default normalization steps recommended by GeneSpring software were used:
| Sample_data_processing | (1) data transformation: set measurements less than 0.01 to 0.01
| Sample_data_processing | (2) per chip: normalize to 50th percentile
| Sample_data_processing | (3) per gene: normalize to median
| Sample_platform_id | GPL570
| Sample_contact_name | DONG-SOON,,BAE
| Sample_contact_email | phmdxb@gwumc.edu
| Sample_contact_phone | 202-994-0964
| Sample_contact_fax | 202-994-2870
| Sample_contact_department | Pharmacology & Physiology
| Sample_contact_institute | George Washinton Uni.
| Sample_contact_address | 2300 I Street, NW
| Sample_contact_city | Washington
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20037
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307077/suppl/GSM307077.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307077/suppl/GSM307077.CHP.gz
| Sample_series_id | GSE12205
| Sample_data_row_count | 54675
| |
|
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Select GSMs and click on "Add groups" |
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