Search results for the GEO ID: GSE12261  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM307999 | GPL570 |
|
Control 4h culture, biological rep1
|
human aortic smooth muscle cells (HASMCs)
|
control
4h
|
Gene expression data from control cultures (4h, vehicle)
|
| Sample_geo_accession | GSM307999
| | Sample_status | Public on Dec 31 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultures were treated with 0.0 (vehicle) or 1.0 uM 2-ME in the presence of 2.5% fetal calf serum (to stimulate proliferation) for 4 hours (acute phase response/early gene induction) and for 30 hrs (late phase).
| | Sample_growth_protocol_ch1 | HASMCs were grown to subconfluence and growth arrested for 24 hrs
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNAeasy Mini kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® U_133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Normalization and computation of expression values was performed using the Robust Multichip Average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Zaugg
| | Sample_contact_email | michael.zaugg@usz.ch
| | Sample_contact_phone | +41-1-255 11 11
| | Sample_contact_laboratory | Perioperative Cardioprotection Research Group
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | Anesthesiology, University Hospital Zurich
| | Sample_contact_address | Raemistrasse 100
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | 8091
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM307nnn/GSM307999/suppl/GSM307999.CEL.gz
| | Sample_series_id | GSE12261
| | Sample_data_row_count | 54675
| | |
|
GSM308000 | GPL570 |
|
Control 4h culture, biological rep2
|
human aortic smooth muscle cells (HASMCs)
|
control
4h
|
Gene expression data from control cultures (4h, vehicle)
|
| Sample_geo_accession | GSM308000
| | Sample_status | Public on Dec 31 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultures were treated with 0.0 (vehicle) or 1.0 uM 2-ME in the presence of 2.5% fetal calf serum (to stimulate proliferation) for 4 hours (acute phase response/early gene induction) and for 30 hrs (late phase).
| | Sample_growth_protocol_ch1 | HASMCs were grown to subconfluence and growth arrested for 24 hrs
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNAeasy Mini kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® U_133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Normalization and computation of expression values was performed using the Robust Multichip Average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Zaugg
| | Sample_contact_email | michael.zaugg@usz.ch
| | Sample_contact_phone | +41-1-255 11 11
| | Sample_contact_laboratory | Perioperative Cardioprotection Research Group
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | Anesthesiology, University Hospital Zurich
| | Sample_contact_address | Raemistrasse 100
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | 8091
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308000/suppl/GSM308000.CEL.gz
| | Sample_series_id | GSE12261
| | Sample_data_row_count | 54675
| | |
|
GSM308001 | GPL570 |
|
Control 4h culture, biological rep3
|
human aortic smooth muscle cells (HASMCs)
|
control
4h
|
Gene expression data from control cultures (4h, vehicle)
|
| Sample_geo_accession | GSM308001
| | Sample_status | Public on Dec 31 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultures were treated with 0.0 (vehicle) or 1.0 uM 2-ME in the presence of 2.5% fetal calf serum (to stimulate proliferation) for 4 hours (acute phase response/early gene induction) and for 30 hrs (late phase).
| | Sample_growth_protocol_ch1 | HASMCs were grown to subconfluence and growth arrested for 24 hrs
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNAeasy Mini kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® U_133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Normalization and computation of expression values was performed using the Robust Multichip Average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Zaugg
| | Sample_contact_email | michael.zaugg@usz.ch
| | Sample_contact_phone | +41-1-255 11 11
| | Sample_contact_laboratory | Perioperative Cardioprotection Research Group
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | Anesthesiology, University Hospital Zurich
| | Sample_contact_address | Raemistrasse 100
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | 8091
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308001/suppl/GSM308001.CEL.gz
| | Sample_series_id | GSE12261
| | Sample_data_row_count | 54675
| | |
|
GSM308002 | GPL570 |
|
ME-treated 4h culture, biological rep1
|
human aortic smooth muscle cells (HASMCs)
|
3uM
4h
|
Gene expression data from control cultures (4h, 2-ME)
|
| Sample_geo_accession | GSM308002
| | Sample_status | Public on Dec 31 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultures were treated with 0.0 (vehicle) or 1.0 uM 2-ME in the presence of 2.5% fetal calf serum (to stimulate proliferation) for 4 hours (acute phase response/early gene induction) and for 30 hrs (late phase).
| | Sample_growth_protocol_ch1 | HASMCs were grown to subconfluence and growth arrested for 24 hrs
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNAeasy Mini kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® U_133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Normalization and computation of expression values was performed using the Robust Multichip Average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Zaugg
| | Sample_contact_email | michael.zaugg@usz.ch
| | Sample_contact_phone | +41-1-255 11 11
| | Sample_contact_laboratory | Perioperative Cardioprotection Research Group
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | Anesthesiology, University Hospital Zurich
| | Sample_contact_address | Raemistrasse 100
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | 8091
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308002/suppl/GSM308002.CEL.gz
| | Sample_series_id | GSE12261
| | Sample_data_row_count | 54675
| | |
|
GSM308003 | GPL570 |
|
ME-treated 4h culture, biological rep2
|
human aortic smooth muscle cells (HASMCs)
|
3uM
4h
|
Gene expression data from control cultures (4h, 2-ME)
|
| Sample_geo_accession | GSM308003
| | Sample_status | Public on Dec 31 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultures were treated with 0.0 (vehicle) or 1.0 uM 2-ME in the presence of 2.5% fetal calf serum (to stimulate proliferation) for 4 hours (acute phase response/early gene induction) and for 30 hrs (late phase).
| | Sample_growth_protocol_ch1 | HASMCs were grown to subconfluence and growth arrested for 24 hrs
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNAeasy Mini kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® U_133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Normalization and computation of expression values was performed using the Robust Multichip Average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Zaugg
| | Sample_contact_email | michael.zaugg@usz.ch
| | Sample_contact_phone | +41-1-255 11 11
| | Sample_contact_laboratory | Perioperative Cardioprotection Research Group
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | Anesthesiology, University Hospital Zurich
| | Sample_contact_address | Raemistrasse 100
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | 8091
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308003/suppl/GSM308003.CEL.gz
| | Sample_series_id | GSE12261
| | Sample_data_row_count | 54675
| | |
|
GSM308004 | GPL570 |
|
ME-treated 4h culture, biological rep3
|
human aortic smooth muscle cells (HASMCs)
|
3uM
4h
|
Gene expression data from control cultures (4h, 2-ME)
|
| Sample_geo_accession | GSM308004
| | Sample_status | Public on Dec 31 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultures were treated with 0.0 (vehicle) or 1.0 uM 2-ME in the presence of 2.5% fetal calf serum (to stimulate proliferation) for 4 hours (acute phase response/early gene induction) and for 30 hrs (late phase).
| | Sample_growth_protocol_ch1 | HASMCs were grown to subconfluence and growth arrested for 24 hrs
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNAeasy Mini kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® U_133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Normalization and computation of expression values was performed using the Robust Multichip Average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Zaugg
| | Sample_contact_email | michael.zaugg@usz.ch
| | Sample_contact_phone | +41-1-255 11 11
| | Sample_contact_laboratory | Perioperative Cardioprotection Research Group
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | Anesthesiology, University Hospital Zurich
| | Sample_contact_address | Raemistrasse 100
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | 8091
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308004/suppl/GSM308004.CEL.gz
| | Sample_series_id | GSE12261
| | Sample_data_row_count | 54675
| | |
|
GSM308005 | GPL570 |
|
Control 30 h culture, biological rep1
|
human aortic smooth muscle cells (HASMCs)
|
control
30h
|
Gene expression data from control cultures (30h, vehicle)
|
| Sample_geo_accession | GSM308005
| | Sample_status | Public on Dec 31 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultures were treated with 0.0 (vehicle) or 1.0 uM 2-ME in the presence of 2.5% fetal calf serum (to stimulate proliferation) for 4 hours (acute phase response/early gene induction) and for 30 hrs (late phase).
| | Sample_growth_protocol_ch1 | HASMCs were grown to subconfluence and growth arrested for 24 hrs
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNAeasy Mini kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® U_133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Normalization and computation of expression values was performed using the Robust Multichip Average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Zaugg
| | Sample_contact_email | michael.zaugg@usz.ch
| | Sample_contact_phone | +41-1-255 11 11
| | Sample_contact_laboratory | Perioperative Cardioprotection Research Group
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | Anesthesiology, University Hospital Zurich
| | Sample_contact_address | Raemistrasse 100
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | 8091
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308005/suppl/GSM308005.CEL.gz
| | Sample_series_id | GSE12261
| | Sample_data_row_count | 54675
| | |
|
GSM308006 | GPL570 |
|
Control 30 h culture, biological rep2
|
human aortic smooth muscle cells (HASMCs)
|
control
30h
|
Gene expression data from control cultures (30h, vehicle)
|
| Sample_geo_accession | GSM308006
| | Sample_status | Public on Dec 31 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultures were treated with 0.0 (vehicle) or 1.0 uM 2-ME in the presence of 2.5% fetal calf serum (to stimulate proliferation) for 4 hours (acute phase response/early gene induction) and for 30 hrs (late phase).
| | Sample_growth_protocol_ch1 | HASMCs were grown to subconfluence and growth arrested for 24 hrs
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNAeasy Mini kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® U_133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Normalization and computation of expression values was performed using the Robust Multichip Average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Zaugg
| | Sample_contact_email | michael.zaugg@usz.ch
| | Sample_contact_phone | +41-1-255 11 11
| | Sample_contact_laboratory | Perioperative Cardioprotection Research Group
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | Anesthesiology, University Hospital Zurich
| | Sample_contact_address | Raemistrasse 100
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | 8091
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308006/suppl/GSM308006.CEL.gz
| | Sample_series_id | GSE12261
| | Sample_data_row_count | 54675
| | |
|
GSM308007 | GPL570 |
|
Control 30 h culture, biological rep3
|
human aortic smooth muscle cells (HASMCs)
|
control
30h
|
Gene expression data from control cultures (30h, vehicle)
|
| Sample_geo_accession | GSM308007
| | Sample_status | Public on Dec 31 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultures were treated with 0.0 (vehicle) or 1.0 uM 2-ME in the presence of 2.5% fetal calf serum (to stimulate proliferation) for 4 hours (acute phase response/early gene induction) and for 30 hrs (late phase).
| | Sample_growth_protocol_ch1 | HASMCs were grown to subconfluence and growth arrested for 24 hrs
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNAeasy Mini kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® U_133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Normalization and computation of expression values was performed using the Robust Multichip Average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Zaugg
| | Sample_contact_email | michael.zaugg@usz.ch
| | Sample_contact_phone | +41-1-255 11 11
| | Sample_contact_laboratory | Perioperative Cardioprotection Research Group
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | Anesthesiology, University Hospital Zurich
| | Sample_contact_address | Raemistrasse 100
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | 8091
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308007/suppl/GSM308007.CEL.gz
| | Sample_series_id | GSE12261
| | Sample_data_row_count | 54675
| | |
|
GSM308008 | GPL570 |
|
ME-treated 30 h culture, biological rep2
|
human aortic smooth muscle cells (HASMCs)
|
3uM
30h
|
Gene expression data from control cultures (30h, 2-ME)
|
| Sample_geo_accession | GSM308008
| | Sample_status | Public on Dec 31 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultures were treated with 0.0 (vehicle) or 1.0 uM 2-ME in the presence of 2.5% fetal calf serum (to stimulate proliferation) for 4 hours (acute phase response/early gene induction) and for 30 hrs (late phase).
| | Sample_growth_protocol_ch1 | HASMCs were grown to subconfluence and growth arrested for 24 hrs
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNAeasy Mini kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® U_133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Normalization and computation of expression values was performed using the Robust Multichip Average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Zaugg
| | Sample_contact_email | michael.zaugg@usz.ch
| | Sample_contact_phone | +41-1-255 11 11
| | Sample_contact_laboratory | Perioperative Cardioprotection Research Group
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | Anesthesiology, University Hospital Zurich
| | Sample_contact_address | Raemistrasse 100
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | 8091
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308008/suppl/GSM308008.CEL.gz
| | Sample_series_id | GSE12261
| | Sample_data_row_count | 54675
| | |
|
GSM308009 | GPL570 |
|
ME-treated 30 h culture, biological rep3
|
human aortic smooth muscle cells (HASMCs)
|
3uM
30h
|
Gene expression data from control cultures (30h, 2-ME)
|
| Sample_geo_accession | GSM308009
| | Sample_status | Public on Dec 31 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultures were treated with 0.0 (vehicle) or 1.0 uM 2-ME in the presence of 2.5% fetal calf serum (to stimulate proliferation) for 4 hours (acute phase response/early gene induction) and for 30 hrs (late phase).
| | Sample_growth_protocol_ch1 | HASMCs were grown to subconfluence and growth arrested for 24 hrs
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNAeasy Mini kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® U_133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Normalization and computation of expression values was performed using the Robust Multichip Average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Zaugg
| | Sample_contact_email | michael.zaugg@usz.ch
| | Sample_contact_phone | +41-1-255 11 11
| | Sample_contact_laboratory | Perioperative Cardioprotection Research Group
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | Anesthesiology, University Hospital Zurich
| | Sample_contact_address | Raemistrasse 100
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | 8091
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308009/suppl/GSM308009.CEL.gz
| | Sample_series_id | GSE12261
| | Sample_data_row_count | 54675
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GSM308010 | GPL570 |
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ME-treated 30 h culture, biological rep4
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human aortic smooth muscle cells (HASMCs)
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3uM
30h
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Gene expression data from control cultures (30h, 2-ME)
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| Sample_geo_accession | GSM308010
| | Sample_status | Public on Dec 31 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The cultures were treated with 0.0 (vehicle) or 1.0 uM 2-ME in the presence of 2.5% fetal calf serum (to stimulate proliferation) for 4 hours (acute phase response/early gene induction) and for 30 hrs (late phase).
| | Sample_growth_protocol_ch1 | HASMCs were grown to subconfluence and growth arrested for 24 hrs
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNAeasy Mini kit (Qiagen) according to the manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® U_133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| | Sample_data_processing | Normalization and computation of expression values was performed using the Robust Multichip Average (RMA) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michael,,Zaugg
| | Sample_contact_email | michael.zaugg@usz.ch
| | Sample_contact_phone | +41-1-255 11 11
| | Sample_contact_laboratory | Perioperative Cardioprotection Research Group
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | Anesthesiology, University Hospital Zurich
| | Sample_contact_address | Raemistrasse 100
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | 8091
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308010/suppl/GSM308010.CEL.gz
| | Sample_series_id | GSE12261
| | Sample_data_row_count | 54675
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