Search results for the GEO ID: GSE12266  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM308067 | GPL570 |
|
Confluent hMSC patient 3002 (time course III)
|
hMSC, confluence
|
male
|
gene expression data
|
| Sample_geo_accession | GSM308067
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | hMSC obtained from bone marrow were maintained in alpha MEM, supplemented with 10% FBS, 100 U/mL penicillin, 0.1 mg/mL streptomycin, 100 μmol/L ascorbic acid-2 phosphate and 10-8 M dexamethasone at 37°C in 5% CO2-humidified atmosphere. To obtain the formation of mineral nodules, 10 mM β-glycerophosphate was added to the hMSC cultures following the second confluence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45ºC on GeneChip Human U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix)
| | Sample_data_processing | The data were analyzed with GeneSpring GX software using RMA and global median normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lourdes,,Osaba
| | Sample_contact_email | losaba@progenika.com
| | Sample_contact_fax | 34044064526
| | Sample_contact_institute | Progenika Biopharma
| | Sample_contact_address | Parque Tecnologico de Bizkaia. Ed. 801
| | Sample_contact_city | Derio
| | Sample_contact_zip/postal_code | E-48160
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308067/suppl/GSM308067.CEL.gz
| | Sample_series_id | GSE12266
| | Sample_series_id | GSE12267
| | Sample_data_row_count | 54675
| | |
|
GSM308068 | GPL570 |
|
24 h after mineralization medium addition patient 3002 (time course III)
|
hMSC, mineralization, 24h
|
male
|
gene expression data
|
| Sample_geo_accession | GSM308068
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | hMSC obtained from bone marrow were maintained in alpha MEM, supplemented with 10% FBS, 100 U/mL penicillin, 0.1 mg/mL streptomycin, 100 μmol/L ascorbic acid-2 phosphate and 10-8 M dexamethasone at 37°C in 5% CO2-humidified atmosphere. To obtain the formation of mineral nodules, 10 mM β-glycerophosphate was added to the hMSC cultures following the second confluence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45ºC on GeneChip Human U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix)
| | Sample_data_processing | The data were analyzed with GeneSpring GX software using RMA and global median normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lourdes,,Osaba
| | Sample_contact_email | losaba@progenika.com
| | Sample_contact_fax | 34044064526
| | Sample_contact_institute | Progenika Biopharma
| | Sample_contact_address | Parque Tecnologico de Bizkaia. Ed. 801
| | Sample_contact_city | Derio
| | Sample_contact_zip/postal_code | E-48160
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308068/suppl/GSM308068.CEL.gz
| | Sample_series_id | GSE12266
| | Sample_series_id | GSE12267
| | Sample_data_row_count | 54675
| | |
|
GSM308069 | GPL570 |
|
7 days after mineralization medium addition patient 3002 (time course III)
|
hMSC, mineralization, 7 days
|
male
|
gene expression data
|
| Sample_geo_accession | GSM308069
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | hMSC obtained from bone marrow were maintained in alpha MEM, supplemented with 10% FBS, 100 U/mL penicillin, 0.1 mg/mL streptomycin, 100 μmol/L ascorbic acid-2 phosphate and 10-8 M dexamethasone at 37°C in 5% CO2-humidified atmosphere. To obtain the formation of mineral nodules, 10 mM β-glycerophosphate was added to the hMSC cultures following the second confluence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45ºC on GeneChip Human U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix)
| | Sample_data_processing | The data were analyzed with GeneSpring GX software using RMA and global median normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lourdes,,Osaba
| | Sample_contact_email | losaba@progenika.com
| | Sample_contact_fax | 34044064526
| | Sample_contact_institute | Progenika Biopharma
| | Sample_contact_address | Parque Tecnologico de Bizkaia. Ed. 801
| | Sample_contact_city | Derio
| | Sample_contact_zip/postal_code | E-48160
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308069/suppl/GSM308069.CEL.gz
| | Sample_series_id | GSE12266
| | Sample_series_id | GSE12267
| | Sample_data_row_count | 54675
| | |
|
GSM308070 | GPL570 |
|
10-14 days after mineralization medium addition patient 3002 (time course III)
|
hMSC, mineralization, 10-14 days
|
male
|
gene expression data
|
| Sample_geo_accession | GSM308070
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | hMSC obtained from bone marrow were maintained in alpha MEM, supplemented with 10% FBS, 100 U/mL penicillin, 0.1 mg/mL streptomycin, 100 μmol/L ascorbic acid-2 phosphate and 10-8 M dexamethasone at 37°C in 5% CO2-humidified atmosphere. To obtain the formation of mineral nodules, 10 mM β-glycerophosphate was added to the hMSC cultures following the second confluence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45ºC on GeneChip Human U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix)
| | Sample_data_processing | The data were analyzed with GeneSpring GX software using RMA and global median normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lourdes,,Osaba
| | Sample_contact_email | losaba@progenika.com
| | Sample_contact_fax | 34044064526
| | Sample_contact_institute | Progenika Biopharma
| | Sample_contact_address | Parque Tecnologico de Bizkaia. Ed. 801
| | Sample_contact_city | Derio
| | Sample_contact_zip/postal_code | E-48160
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308070/suppl/GSM308070.CEL.gz
| | Sample_series_id | GSE12266
| | Sample_series_id | GSE12267
| | Sample_data_row_count | 54675
| | |
|
GSM308071 | GPL570 |
|
Confluent hMSC patient 3080 (time course III)
|
hMSC, confluence
|
male
|
gene expression data
|
| Sample_geo_accession | GSM308071
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | hMSC obtained from bone marrow were maintained in alpha MEM, supplemented with 10% FBS, 100 U/mL penicillin, 0.1 mg/mL streptomycin, 100 μmol/L ascorbic acid-2 phosphate and 10-8 M dexamethasone at 37°C in 5% CO2-humidified atmosphere. To obtain the formation of mineral nodules, 10 mM β-glycerophosphate was added to the hMSC cultures following the second confluence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45ºC on GeneChip Human U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix)
| | Sample_data_processing | The data were analyzed with GeneSpring GX software using RMA and global median normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lourdes,,Osaba
| | Sample_contact_email | losaba@progenika.com
| | Sample_contact_fax | 34044064526
| | Sample_contact_institute | Progenika Biopharma
| | Sample_contact_address | Parque Tecnologico de Bizkaia. Ed. 801
| | Sample_contact_city | Derio
| | Sample_contact_zip/postal_code | E-48160
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308071/suppl/GSM308071.CEL.gz
| | Sample_series_id | GSE12266
| | Sample_series_id | GSE12267
| | Sample_data_row_count | 54675
| | |
|
GSM308072 | GPL570 |
|
24 h after mineralization medium addition patient 3080 (time course III)
|
hMSC, mineralization, 24h
|
male
|
gene expression data
|
| Sample_geo_accession | GSM308072
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | hMSC obtained from bone marrow were maintained in alpha MEM, supplemented with 10% FBS, 100 U/mL penicillin, 0.1 mg/mL streptomycin, 100 μmol/L ascorbic acid-2 phosphate and 10-8 M dexamethasone at 37°C in 5% CO2-humidified atmosphere. To obtain the formation of mineral nodules, 10 mM β-glycerophosphate was added to the hMSC cultures following the second confluence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45ºC on GeneChip Human U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix)
| | Sample_data_processing | The data were analyzed with GeneSpring GX software using RMA and global median normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lourdes,,Osaba
| | Sample_contact_email | losaba@progenika.com
| | Sample_contact_fax | 34044064526
| | Sample_contact_institute | Progenika Biopharma
| | Sample_contact_address | Parque Tecnologico de Bizkaia. Ed. 801
| | Sample_contact_city | Derio
| | Sample_contact_zip/postal_code | E-48160
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308072/suppl/GSM308072.CEL.gz
| | Sample_series_id | GSE12266
| | Sample_series_id | GSE12267
| | Sample_data_row_count | 54675
| | |
|
GSM308073 | GPL570 |
|
7 days after mineralization medium addition patient 3080 (time course III)
|
hMSC, mineralization, 7 days
|
male
|
gene expression data
|
| Sample_geo_accession | GSM308073
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | hMSC obtained from bone marrow were maintained in alpha MEM, supplemented with 10% FBS, 100 U/mL penicillin, 0.1 mg/mL streptomycin, 100 μmol/L ascorbic acid-2 phosphate and 10-8 M dexamethasone at 37°C in 5% CO2-humidified atmosphere. To obtain the formation of mineral nodules, 10 mM β-glycerophosphate was added to the hMSC cultures following the second confluence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45ºC on GeneChip Human U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix)
| | Sample_data_processing | The data were analyzed with GeneSpring GX software using RMA and global median normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lourdes,,Osaba
| | Sample_contact_email | losaba@progenika.com
| | Sample_contact_fax | 34044064526
| | Sample_contact_institute | Progenika Biopharma
| | Sample_contact_address | Parque Tecnologico de Bizkaia. Ed. 801
| | Sample_contact_city | Derio
| | Sample_contact_zip/postal_code | E-48160
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308073/suppl/GSM308073.CEL.gz
| | Sample_series_id | GSE12266
| | Sample_series_id | GSE12267
| | Sample_data_row_count | 54675
| | |
|
GSM308074 | GPL570 |
|
10-14 days after mineralization medium addition patient 3080 (time course III)
|
hMSC, mineralization, 10-14 days
|
male
|
gene expression data
|
| Sample_geo_accession | GSM308074
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | hMSC obtained from bone marrow were maintained in alpha MEM, supplemented with 10% FBS, 100 U/mL penicillin, 0.1 mg/mL streptomycin, 100 μmol/L ascorbic acid-2 phosphate and 10-8 M dexamethasone at 37°C in 5% CO2-humidified atmosphere. To obtain the formation of mineral nodules, 10 mM β-glycerophosphate was added to the hMSC cultures following the second confluence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45ºC on GeneChip Human U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix)
| | Sample_data_processing | The data were analyzed with GeneSpring GX software using RMA and global median normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lourdes,,Osaba
| | Sample_contact_email | losaba@progenika.com
| | Sample_contact_fax | 34044064526
| | Sample_contact_institute | Progenika Biopharma
| | Sample_contact_address | Parque Tecnologico de Bizkaia. Ed. 801
| | Sample_contact_city | Derio
| | Sample_contact_zip/postal_code | E-48160
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308074/suppl/GSM308074.CEL.gz
| | Sample_series_id | GSE12266
| | Sample_series_id | GSE12267
| | Sample_data_row_count | 54675
| | |
|
GSM308075 | GPL570 |
|
Confluent hMSC patient 3205 (time course III)
|
hMSC, confluence
|
male
|
gene expression data
|
| Sample_geo_accession | GSM308075
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | hMSC obtained from bone marrow were maintained in alpha MEM, supplemented with 10% FBS, 100 U/mL penicillin, 0.1 mg/mL streptomycin, 100 μmol/L ascorbic acid-2 phosphate and 10-8 M dexamethasone at 37°C in 5% CO2-humidified atmosphere. To obtain the formation of mineral nodules, 10 mM β-glycerophosphate was added to the hMSC cultures following the second confluence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45ºC on GeneChip Human U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix)
| | Sample_data_processing | The data were analyzed with GeneSpring GX software using RMA and global median normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lourdes,,Osaba
| | Sample_contact_email | losaba@progenika.com
| | Sample_contact_fax | 34044064526
| | Sample_contact_institute | Progenika Biopharma
| | Sample_contact_address | Parque Tecnologico de Bizkaia. Ed. 801
| | Sample_contact_city | Derio
| | Sample_contact_zip/postal_code | E-48160
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308075/suppl/GSM308075.CEL.gz
| | Sample_series_id | GSE12266
| | Sample_series_id | GSE12267
| | Sample_data_row_count | 54675
| | |
|
GSM308076 | GPL570 |
|
24 h after mineralization medium addition patient 3205 (time course III)
|
hMSC, mineralization, 24h
|
male
|
gene expression data
|
| Sample_geo_accession | GSM308076
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | hMSC obtained from bone marrow were maintained in alpha MEM, supplemented with 10% FBS, 100 U/mL penicillin, 0.1 mg/mL streptomycin, 100 μmol/L ascorbic acid-2 phosphate and 10-8 M dexamethasone at 37°C in 5% CO2-humidified atmosphere. To obtain the formation of mineral nodules, 10 mM β-glycerophosphate was added to the hMSC cultures following the second confluence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45ºC on GeneChip Human U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix)
| | Sample_data_processing | The data were analyzed with GeneSpring GX software using RMA and global median normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lourdes,,Osaba
| | Sample_contact_email | losaba@progenika.com
| | Sample_contact_fax | 34044064526
| | Sample_contact_institute | Progenika Biopharma
| | Sample_contact_address | Parque Tecnologico de Bizkaia. Ed. 801
| | Sample_contact_city | Derio
| | Sample_contact_zip/postal_code | E-48160
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308076/suppl/GSM308076.CEL.gz
| | Sample_series_id | GSE12266
| | Sample_series_id | GSE12267
| | Sample_data_row_count | 54675
| | |
|
GSM308077 | GPL570 |
|
7 days after mineralization medium addition patient 3205 (time course III)
|
hMSC, mineralization, 7 days
|
male
|
gene expression data
|
| Sample_geo_accession | GSM308077
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | hMSC obtained from bone marrow were maintained in alpha MEM, supplemented with 10% FBS, 100 U/mL penicillin, 0.1 mg/mL streptomycin, 100 μmol/L ascorbic acid-2 phosphate and 10-8 M dexamethasone at 37°C in 5% CO2-humidified atmosphere. To obtain the formation of mineral nodules, 10 mM β-glycerophosphate was added to the hMSC cultures following the second confluence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45ºC on GeneChip Human U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix)
| | Sample_data_processing | The data were analyzed with GeneSpring GX software using RMA and global median normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lourdes,,Osaba
| | Sample_contact_email | losaba@progenika.com
| | Sample_contact_fax | 34044064526
| | Sample_contact_institute | Progenika Biopharma
| | Sample_contact_address | Parque Tecnologico de Bizkaia. Ed. 801
| | Sample_contact_city | Derio
| | Sample_contact_zip/postal_code | E-48160
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308077/suppl/GSM308077.CEL.gz
| | Sample_series_id | GSE12266
| | Sample_series_id | GSE12267
| | Sample_data_row_count | 54675
| | |
|
GSM308078 | GPL570 |
|
10-14 days after mineralization medium addition patient 3205 (time course III)
|
hMSC, mineralization, 10-14 days
|
male
|
gene expression data
|
| Sample_geo_accession | GSM308078
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | hMSC obtained from bone marrow were maintained in alpha MEM, supplemented with 10% FBS, 100 U/mL penicillin, 0.1 mg/mL streptomycin, 100 μmol/L ascorbic acid-2 phosphate and 10-8 M dexamethasone at 37°C in 5% CO2-humidified atmosphere. To obtain the formation of mineral nodules, 10 mM β-glycerophosphate was added to the hMSC cultures following the second confluence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45ºC on GeneChip Human U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix)
| | Sample_data_processing | The data were analyzed with GeneSpring GX software using RMA and global median normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lourdes,,Osaba
| | Sample_contact_email | losaba@progenika.com
| | Sample_contact_fax | 34044064526
| | Sample_contact_institute | Progenika Biopharma
| | Sample_contact_address | Parque Tecnologico de Bizkaia. Ed. 801
| | Sample_contact_city | Derio
| | Sample_contact_zip/postal_code | E-48160
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308078/suppl/GSM308078.CEL.gz
| | Sample_series_id | GSE12266
| | Sample_series_id | GSE12267
| | Sample_data_row_count | 54675
| | |
|
GSM308079 | GPL570 |
|
Confluent hMSC patient 3206 (time course III)
|
hMSC, confluence
|
male
|
gene expression data
|
| Sample_geo_accession | GSM308079
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | hMSC obtained from bone marrow were maintained in alpha MEM, supplemented with 10% FBS, 100 U/mL penicillin, 0.1 mg/mL streptomycin, 100 μmol/L ascorbic acid-2 phosphate and 10-8 M dexamethasone at 37°C in 5% CO2-humidified atmosphere. To obtain the formation of mineral nodules, 10 mM β-glycerophosphate was added to the hMSC cultures following the second confluence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45ºC on GeneChip Human U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix)
| | Sample_data_processing | The data were analyzed with GeneSpring GX software using RMA and global median normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lourdes,,Osaba
| | Sample_contact_email | losaba@progenika.com
| | Sample_contact_fax | 34044064526
| | Sample_contact_institute | Progenika Biopharma
| | Sample_contact_address | Parque Tecnologico de Bizkaia. Ed. 801
| | Sample_contact_city | Derio
| | Sample_contact_zip/postal_code | E-48160
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308079/suppl/GSM308079.CEL.gz
| | Sample_series_id | GSE12266
| | Sample_series_id | GSE12267
| | Sample_data_row_count | 54675
| | |
|
GSM308080 | GPL570 |
|
24 h after mineralization medium addition patient 3206 (time course III)
|
hMSC, mineralization, 24h
|
male
|
gene expression data
|
| Sample_geo_accession | GSM308080
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | hMSC obtained from bone marrow were maintained in alpha MEM, supplemented with 10% FBS, 100 U/mL penicillin, 0.1 mg/mL streptomycin, 100 μmol/L ascorbic acid-2 phosphate and 10-8 M dexamethasone at 37°C in 5% CO2-humidified atmosphere. To obtain the formation of mineral nodules, 10 mM β-glycerophosphate was added to the hMSC cultures following the second confluence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45ºC on GeneChip Human U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix)
| | Sample_data_processing | The data were analyzed with GeneSpring GX software using RMA and global median normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lourdes,,Osaba
| | Sample_contact_email | losaba@progenika.com
| | Sample_contact_fax | 34044064526
| | Sample_contact_institute | Progenika Biopharma
| | Sample_contact_address | Parque Tecnologico de Bizkaia. Ed. 801
| | Sample_contact_city | Derio
| | Sample_contact_zip/postal_code | E-48160
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308080/suppl/GSM308080.CEL.gz
| | Sample_series_id | GSE12266
| | Sample_series_id | GSE12267
| | Sample_data_row_count | 54675
| | |
|
GSM308081 | GPL570 |
|
7 days after mineralization medium addition patient 3206 (time course III)
|
hMSC, mineralization, 7 days
|
male
|
gene expression data
|
| Sample_geo_accession | GSM308081
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | hMSC obtained from bone marrow were maintained in alpha MEM, supplemented with 10% FBS, 100 U/mL penicillin, 0.1 mg/mL streptomycin, 100 μmol/L ascorbic acid-2 phosphate and 10-8 M dexamethasone at 37°C in 5% CO2-humidified atmosphere. To obtain the formation of mineral nodules, 10 mM β-glycerophosphate was added to the hMSC cultures following the second confluence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45ºC on GeneChip Human U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix)
| | Sample_data_processing | The data were analyzed with GeneSpring GX software using RMA and global median normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lourdes,,Osaba
| | Sample_contact_email | losaba@progenika.com
| | Sample_contact_fax | 34044064526
| | Sample_contact_institute | Progenika Biopharma
| | Sample_contact_address | Parque Tecnologico de Bizkaia. Ed. 801
| | Sample_contact_city | Derio
| | Sample_contact_zip/postal_code | E-48160
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308081/suppl/GSM308081.CEL.gz
| | Sample_series_id | GSE12266
| | Sample_series_id | GSE12267
| | Sample_data_row_count | 54675
| | |
|
GSM308082 | GPL570 |
|
10-14 days after mineralization medium addition patient 3206 (time course III)
|
hMSC, mineralization, 10-14 days
|
male
|
gene expression data
|
| Sample_geo_accession | GSM308082
| | Sample_status | Public on Jul 29 2008
| | Sample_submission_date | Jul 28 2008
| | Sample_last_update_date | Jul 28 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | hMSC obtained from bone marrow were maintained in alpha MEM, supplemented with 10% FBS, 100 U/mL penicillin, 0.1 mg/mL streptomycin, 100 μmol/L ascorbic acid-2 phosphate and 10-8 M dexamethasone at 37°C in 5% CO2-humidified atmosphere. To obtain the formation of mineral nodules, 10 mM β-glycerophosphate was added to the hMSC cultures following the second confluence.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Rneasy Mini kit according to manufacturer's instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45ºC on GeneChip Human U133Plus2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix)
| | Sample_data_processing | The data were analyzed with GeneSpring GX software using RMA and global median normalization method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Lourdes,,Osaba
| | Sample_contact_email | losaba@progenika.com
| | Sample_contact_fax | 34044064526
| | Sample_contact_institute | Progenika Biopharma
| | Sample_contact_address | Parque Tecnologico de Bizkaia. Ed. 801
| | Sample_contact_city | Derio
| | Sample_contact_zip/postal_code | E-48160
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM308nnn/GSM308082/suppl/GSM308082.CEL.gz
| | Sample_series_id | GSE12266
| | Sample_series_id | GSE12267
| | Sample_data_row_count | 54675
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