Search results for the GEO ID: GSE12309  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM309471 | GPL1261 |
|
MAFB_E15.5_1
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E15.5 Podocyte Layer
|
gene reported: Tg(Mafb-EGFP)79G / MMRRC:011834-UCD
strain: CD-1
sex: unknown
developmental stage: E15.5
theiler stage: 23
somite count: ND
developmental landmark:
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:10711
|
| Sample_geo_accession | GSM309471
| | Sample_status | Public on Aug 04 2008
| | Sample_submission_date | Jul 31 2008
| | Sample_last_update_date | Dec 31 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | pool size: approx 5-10 E15.5 kidneys
| | Sample_treatment_protocol_ch1 | Pooled sample: Yes
| | Sample_treatment_protocol_ch1 | Dissection Method: Podocyte cells were isolated from E15.5 Mafb transgenic mice kidneys using a combination of tyrpsinization and FACS.
| | Sample_treatment_protocol_ch1 | Mafb transgenic mice are time-mated. Pregnant Mafb transgenic mice are euthanized by standard carbon dioxide asphyxiation. All fetuses are killed by decapitation with a scalpel. Fetal kidneys are dissected from fetuses and placed in ice-cold PBS. Embryonic kidneys are incubated in the presence of 300µl of trypsin for 5 minutes at 37 ºC. Kidneys are then dissociated by titurating in the presence of 600µl of ice-cold 10%FBS/PBS. The dissociated cells from the kidneys are pelleted at 5000 rpm, 4 ºC for 5 minutes. The media is aspirated and the cell pellet is resuspended in 200µl of ice-cold 2%FBS/PBS. Filter cells through 70 micron mesh filter and the GFP positive cells are further isolated using FACS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Potter protocol: 'RNA purification'
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_hyb_protocol | Amount labeled target hybridization to array: 5ug
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | GUDMAP,,Developers
| | Sample_contact_email | gudmap-editors@gudmap.org
| | Sample_contact_phone | +44 131 3322471
| | Sample_contact_laboratory | GUDMAP Database Group
| | Sample_contact_institute | MRC Human Genetics Unit
| | Sample_contact_address | Crewe Road
| | Sample_contact_city | Edinburgh
| | Sample_contact_zip/postal_code | EH4 2XU
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM309nnn/GSM309471/suppl/GSM309471.CEL.gz
| | Sample_series_id | GSE12309
| | Sample_data_row_count | 45101
| | |
|
GSM309472 | GPL1261 |
|
MAFB_E15.5_2
|
E15.5 Podocyte Layer
|
gene reported: Tg(Mafb-EGFP)79G / MMRRC:011834-UCD
strain: CD-1
sex: unknown
developmental stage: E15.5
theiler stage: 23
somite count: ND
developmental landmark:
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:10712
|
| Sample_geo_accession | GSM309472
| | Sample_status | Public on Aug 04 2008
| | Sample_submission_date | Jul 31 2008
| | Sample_last_update_date | Dec 31 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | pool size: approx 5-10 E15.5 kidneys
| | Sample_treatment_protocol_ch1 | Pooled sample: Yes
| | Sample_treatment_protocol_ch1 | Dissection Method: Podocyte cells were isolated from E15.5 Mafb transgenic mice kidneys using a combination of tyrpsinization and FACS.
| | Sample_treatment_protocol_ch1 | Mafb transgenic mice are time-mated. Pregnant Mafb transgenic mice are euthanized by standard carbon dioxide asphyxiation. All fetuses are killed by decapitation with a scalpel. Fetal kidneys are dissected from fetuses and placed in ice-cold PBS. Embryonic kidneys are incubated in the presence of 300µl of trypsin for 5 minutes at 37 ºC. Kidneys are then dissociated by titurating in the presence of 600µl of ice-cold 10%FBS/PBS. The dissociated cells from the kidneys are pelleted at 5000 rpm, 4 ºC for 5 minutes. The media is aspirated and the cell pellet is resuspended in 200µl of ice-cold 2%FBS/PBS. Filter cells through 70 micron mesh filter and the GFP positive cells are further isolated using FACS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Potter protocol: 'RNA purification'
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_hyb_protocol | Amount labeled target hybridization to array: 5ug
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | GUDMAP,,Developers
| | Sample_contact_email | gudmap-editors@gudmap.org
| | Sample_contact_phone | +44 131 3322471
| | Sample_contact_laboratory | GUDMAP Database Group
| | Sample_contact_institute | MRC Human Genetics Unit
| | Sample_contact_address | Crewe Road
| | Sample_contact_city | Edinburgh
| | Sample_contact_zip/postal_code | EH4 2XU
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM309nnn/GSM309472/suppl/GSM309472.CEL.gz
| | Sample_series_id | GSE12309
| | Sample_data_row_count | 45101
| | |
|
GSM309473 | GPL1261 |
|
MAFB_E15.5_3
|
E15.5 Podocyte Layer
|
gene reported: Tg(Mafb-EGFP)79Gsat / MMRRC:011834-UCD
strain: CD-1
sex: unknown
developmental stage: E15.5
theiler stage: 23
somite count: ND
developmental landmark:
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:10713
|
| Sample_geo_accession | GSM309473
| | Sample_status | Public on Aug 04 2008
| | Sample_submission_date | Jul 31 2008
| | Sample_last_update_date | Dec 31 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | pool size: approx 5-10 E15.5 kidneys
| | Sample_treatment_protocol_ch1 | Pooled sample: Yes
| | Sample_treatment_protocol_ch1 | Dissection Method: Podocyte cells were isolated from E15.5 Mafb transgenic mice kidneys using a combination of tyrpsinization and FACS.
| | Sample_treatment_protocol_ch1 | Mafb transgenic mice are time-mated. Pregnant Mafb transgenic mice are euthanized by standard carbon dioxide asphyxiation. All fetuses are killed by decapitation with a scalpel. Fetal kidneys are dissected from fetuses and placed in ice-cold PBS. Embryonic kidneys are incubated in the presence of 300µl of trypsin for 5 minutes at 37 ºC. Kidneys are then dissociated by titurating in the presence of 600µl of ice-cold 10%FBS/PBS. The dissociated cells from the kidneys are pelleted at 5000 rpm, 4 ºC for 5 minutes. The media is aspirated and the cell pellet is resuspended in 200µl of ice-cold 2%FBS/PBS. Filter cells through 70 micron mesh filter and the GFP positive cells are further isolated using FACS.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Potter protocol: 'RNA purification'
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_hyb_protocol | Amount labeled target hybridization to array: 5ug
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | GUDMAP,,Developers
| | Sample_contact_email | gudmap-editors@gudmap.org
| | Sample_contact_phone | +44 131 3322471
| | Sample_contact_laboratory | GUDMAP Database Group
| | Sample_contact_institute | MRC Human Genetics Unit
| | Sample_contact_address | Crewe Road
| | Sample_contact_city | Edinburgh
| | Sample_contact_zip/postal_code | EH4 2XU
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM309nnn/GSM309473/suppl/GSM309473.CEL.gz
| | Sample_series_id | GSE12309
| | Sample_data_row_count | 45101
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