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Search results for the GEO ID: GSE12333
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GPL ID
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Title
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Description
Characteristics
GSM309928
GPL1261
embryoid body, microspheres, control, 10 days, replicate #1 embryoid body, microspheres, control, 10 days, replicate #1 ESCs (D3 line) were maintained in an undifferentiated state on gelatin-coated tissue culture plates in LIF-containing media as described previously. EBs were formed from 2x106 ESCs inoculated in LIF-free media and cultured under rotary conditions. EBs containing microspheres were produced by coating CellTracker Red or RA-loaded microspheres in 0.1% gelatin solution for 3 hours prior to mixing with ESCs in various microsphere to cell ratios and a range of rotary speeds. EB media was exchanged every 1-2 days as needed. CEL and CHP files were generated by the Affymetrix Gene Chip Operating System (GCOS) default settings.
GSM309929
GPL1261
embryoid body, microspheres, control, 10 days, replicate #2 embryoid body, microspheres, control, 10 days, replicate #2 ESCs (D3 line) were maintained in an undifferentiated state on gelatin-coated tissue culture plates in LIF-containing media as described previously. EBs were formed from 2x106 ESCs inoculated in LIF-free media and cultured under rotary conditions. EBs containing microspheres were produced by coating CellTracker Red or RA-loaded microspheres in 0.1% gelatin solution for 3 hours prior to mixing with ESCs in various microsphere to cell ratios and a range of rotary speeds. EB media was exchanged every 1-2 days as needed. CEL and CHP files were generated by the Affymetrix Gene Chip Operating System (GCOS) default settings.
GSM309930
GPL1261
embryoid body, microspheres, control, 10 days, replicate #3 embryoid body, microspheres, control, 10 days, replicate #3 ESCs (D3 line) were maintained in an undifferentiated state on gelatin-coated tissue culture plates in LIF-containing media as described previously. EBs were formed from 2x106 ESCs inoculated in LIF-free media and cultured under rotary conditions. EBs containing microspheres were produced by coating CellTracker Red or RA-loaded microspheres in 0.1% gelatin solution for 3 hours prior to mixing with ESCs in various microsphere to cell ratios and a range of rotary speeds. EB media was exchanged every 1-2 days as needed. CEL and CHP files were generated by the Affymetrix Gene Chip Operating System (GCOS) default settings.
GSM309931
GPL1261
embryoid body, microspheres containing Retinoic Acid, 10 days, replicate #1 embryoid body, microspheres containing Retinoic Acid, 10 days, replicate #1 ESCs (D3 line) were maintained in an undifferentiated state on gelatin-coated tissue culture plates in LIF-containing media as described previously. EBs were formed from 2x106 ESCs inoculated in LIF-free media and cultured under rotary conditions. EBs containing microspheres were produced by coating CellTracker Red or RA-loaded microspheres in 0.1% gelatin solution for 3 hours prior to mixing with ESCs in various microsphere to cell ratios and a range of rotary speeds. EB media was exchanged every 1-2 days as needed. CEL and CHP files were generated by the Affymetrix Gene Chip Operating System (GCOS) default settings.
GSM309932
GPL1261
embryoid body, microspheres containing Retinoic Acid, 10 days, replicate #2 embryoid body, microspheres containing Retinoic Acid, 10 days, replicate #2 ESCs (D3 line) were maintained in an undifferentiated state on gelatin-coated tissue culture plates in LIF-containing media as described previously. EBs were formed from 2x106 ESCs inoculated in LIF-free media and cultured under rotary conditions. EBs containing microspheres were produced by coating CellTracker Red or RA-loaded microspheres in 0.1% gelatin solution for 3 hours prior to mixing with ESCs in various microsphere to cell ratios and a range of rotary speeds. EB media was exchanged every 1-2 days as needed. CEL and CHP files were generated by the Affymetrix Gene Chip Operating System (GCOS) default settings.
GSM309933
GPL1261
embryoid body, microspheres containing Retinoic Acid, 10 days, replicate #3 embryoid body, microspheres containing Retinoic Acid, 10 days, replicate #3 ESCs (D3 line) were maintained in an undifferentiated state on gelatin-coated tissue culture plates in LIF-containing media as described previously. EBs were formed from 2x106 ESCs inoculated in LIF-free media and cultured under rotary conditions. EBs containing microspheres were produced by coating CellTracker Red or RA-loaded microspheres in 0.1% gelatin solution for 3 hours prior to mixing with ESCs in various microsphere to cell ratios and a range of rotary speeds. EB media was exchanged every 1-2 days as needed. CEL and CHP files were generated by the Affymetrix Gene Chip Operating System (GCOS) default settings.
 
 
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