Search results for the GEO ID: GSE12336  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM309953 | GPL570 |
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2MLN_GFP
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Xenografted 2MLN-GFP tumors.
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total rna: extracted from xenografted 2MLN-GFP tumors.
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Gene expression analysis of TGF-beta signaling-disrupting gastric cancer cell line 2MLN.
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| Sample_geo_accession | GSM309953
| | Sample_status | Public on Dec 30 2011
| | Sample_submission_date | Aug 05 2008
| | Sample_last_update_date | Dec 30 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2MLN-GFP cells were injected subcutaneously at the left flank of male Balb/c nude mice of 4-5 weeks.
| | Sample_growth_protocol_ch1 | Tumors were allowed to grow for one week.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Tumor tissue was digested with 1 mg/ml collagenase (Worthington, NJ, USA) and 0,25% Trypsin-EDTA (Invitrogen). The resulting single cell suspension was subjected to MACS cell separation using microbeads conjugated to CD326 antibody (Miltenyi Biotec, CA, USA) allowing the selection of CD326 expressing human cancer cells from CD326 negative mouse stromal cells. Total RNA from the isolated cancer cells were purified using the RNeasy Mini Kit (QIAGEN).
| | Sample_label_ch1 | Biotin-labelled.
| | Sample_label_protocol_ch1 | According to Affymetrix's instructions.
| | Sample_hyb_protocol | According to Affymetrix's instructions.
| | Sample_scan_protocol | According to Affymetrix's instructions.
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mitsunobu,R,Kano
| | Sample_contact_email | mikano-tky@umin.net
| | Sample_contact_phone | +81-3-5841-3356
| | Sample_contact_fax | +81-3-5841-3354
| | Sample_contact_laboratory | Department of Molecular Pathology
| | Sample_contact_department | Graduate School of Medicine
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | Hongo 7-3-1
| | Sample_contact_city | Bunkyo-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 113-0033
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM309nnn/GSM309953/suppl/GSM309953.CEL.gz
| | Sample_series_id | GSE12336
| | Sample_data_row_count | 54675
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GSM309954 | GPL570 |
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2MLN_dnTbRII
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Xenografted 2MLN-dnTbRII tumors.
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total rna: extracted from xenografted 2MLN-dnTbRII tumors.
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Gene expression analysis of TGF-beta signaling-disrupting gastric cancer cell line 2MLN.
|
| Sample_geo_accession | GSM309954
| | Sample_status | Public on Dec 30 2011
| | Sample_submission_date | Aug 05 2008
| | Sample_last_update_date | Dec 30 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2MLN-dnTbRII cells were injected subcutaneously at the left flank of male Balb/c nude mice of 4-5 weeks.
| | Sample_growth_protocol_ch1 | Tumors were allowed to grow for one week.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Tumor tissue was digested with 1 mg/ml collagenase (Worthington, NJ, USA) and 0,25% Trypsin-EDTA (Invitrogen). The resulting single cell suspension was subjected to MACS cell separation using microbeads conjugated to CD326 antibody (Miltenyi Biotec, CA, USA) allowing the selection of CD326 expressing human cancer cells from CD326 negative mouse stromal cells. Total RNA from the isolated cancer cells were purified using the RNeasy Mini Kit (QIAGEN).
| | Sample_label_ch1 | Biotin-labelled.
| | Sample_label_protocol_ch1 | According to Affymetrix's instructions.
| | Sample_hyb_protocol | According to Affymetrix's instructions.
| | Sample_scan_protocol | According to Affymetrix's instructions.
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mitsunobu,R,Kano
| | Sample_contact_email | mikano-tky@umin.net
| | Sample_contact_phone | +81-3-5841-3356
| | Sample_contact_fax | +81-3-5841-3354
| | Sample_contact_laboratory | Department of Molecular Pathology
| | Sample_contact_department | Graduate School of Medicine
| | Sample_contact_institute | The University of Tokyo
| | Sample_contact_address | Hongo 7-3-1
| | Sample_contact_city | Bunkyo-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 113-0033
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM309nnn/GSM309954/suppl/GSM309954.CEL.gz
| | Sample_series_id | GSE12336
| | Sample_data_row_count | 54675
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