Search results for the GEO ID: GSE12342  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM310023 | GPL341 |
|
pineal gland, mid-day, rep 1
|
pineal gland, mid-day
|
time: mid-day
strain: Sprague-Dawley
age: 2-3 months
genotype/variation: wild type
|
in vivo pineal gland
|
| Sample_geo_accession | GSM310023
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Aug 05 2008
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Pineal glands were stored at -80oC prior to RNA extraction.
| | Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 18 hr at 45oC to an Affymetrix rat RAE230A GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL341
| | Sample_contact_name | David,C.,Klein
| | Sample_contact_email | kleind@mail.nih.gov
| | Sample_contact_phone | 3014966915
| | Sample_contact_fax | 3014803526
| | Sample_contact_laboratory | Sect. Neuroendo.
| | Sample_contact_department | PDEGEN
| | Sample_contact_institute | NICHD/NIH
| | Sample_contact_address | 49 Convent Dr.
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310023/suppl/GSM310023.CEL.gz
| | Sample_series_id | GSE12342
| | Sample_series_id | GSE12344
| | Sample_data_row_count | 15923
| | |
|
GSM310024 | GPL341 |
|
pineal gland, mid-day, rep 2
|
pineal gland, mid-day
|
time: mid-day
strain: Sprague-Dawley
age: 2-3 months
genotype/variation: wild type
|
in vivo pineal gland
|
| Sample_geo_accession | GSM310024
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Aug 05 2008
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Pineal glands were stored at -80oC prior to RNA extraction.
| | Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 18 hr at 45oC to an Affymetrix rat RAE230A GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL341
| | Sample_contact_name | David,C.,Klein
| | Sample_contact_email | kleind@mail.nih.gov
| | Sample_contact_phone | 3014966915
| | Sample_contact_fax | 3014803526
| | Sample_contact_laboratory | Sect. Neuroendo.
| | Sample_contact_department | PDEGEN
| | Sample_contact_institute | NICHD/NIH
| | Sample_contact_address | 49 Convent Dr.
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310024/suppl/GSM310024.CEL.gz
| | Sample_series_id | GSE12342
| | Sample_series_id | GSE12344
| | Sample_data_row_count | 15923
| | |
|
GSM310025 | GPL341 |
|
pineal gland, mid-day, rep 3
|
pineal gland, mid-day
|
time: mid-day
strain: Sprague-Dawley
age: 2-3 months
genotype/variation: wild type
|
in vivo pineal gland
|
| Sample_geo_accession | GSM310025
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Aug 05 2008
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Pineal glands were stored at -80oC prior to RNA extraction.
| | Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 18 hr at 45oC to an Affymetrix rat RAE230A GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL341
| | Sample_contact_name | David,C.,Klein
| | Sample_contact_email | kleind@mail.nih.gov
| | Sample_contact_phone | 3014966915
| | Sample_contact_fax | 3014803526
| | Sample_contact_laboratory | Sect. Neuroendo.
| | Sample_contact_department | PDEGEN
| | Sample_contact_institute | NICHD/NIH
| | Sample_contact_address | 49 Convent Dr.
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310025/suppl/GSM310025.CEL.gz
| | Sample_series_id | GSE12342
| | Sample_series_id | GSE12344
| | Sample_data_row_count | 15923
| | |
|
GSM310026 | GPL341 |
|
pineal gland, mid-night, rep 1
|
pineal gland, mid-night
|
time: mid-night
strain: Sprague-Dawley
age: 2-3 months
genotype/variation: wild type
|
in vivo pineal gland
|
| Sample_geo_accession | GSM310026
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Aug 05 2008
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Pineal glands were stored at -80oC prior to RNA extraction.
| | Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 18 hr at 45oC to an Affymetrix rat RAE230A GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL341
| | Sample_contact_name | David,C.,Klein
| | Sample_contact_email | kleind@mail.nih.gov
| | Sample_contact_phone | 3014966915
| | Sample_contact_fax | 3014803526
| | Sample_contact_laboratory | Sect. Neuroendo.
| | Sample_contact_department | PDEGEN
| | Sample_contact_institute | NICHD/NIH
| | Sample_contact_address | 49 Convent Dr.
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310026/suppl/GSM310026.CEL.gz
| | Sample_series_id | GSE12342
| | Sample_series_id | GSE12344
| | Sample_data_row_count | 15923
| | |
|
GSM310027 | GPL341 |
|
pineal gland, mid-night, rep 2
|
pineal gland, mid-night
|
time: mid-night
strain: Sprague-Dawley
age: 2-3 months
genotype/variation: wild type
|
in vivo pineal gland
|
| Sample_geo_accession | GSM310027
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Aug 05 2008
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Pineal glands were stored at -80oC prior to RNA extraction.
| | Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 18 hr at 45oC to an Affymetrix rat RAE230A GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL341
| | Sample_contact_name | David,C.,Klein
| | Sample_contact_email | kleind@mail.nih.gov
| | Sample_contact_phone | 3014966915
| | Sample_contact_fax | 3014803526
| | Sample_contact_laboratory | Sect. Neuroendo.
| | Sample_contact_department | PDEGEN
| | Sample_contact_institute | NICHD/NIH
| | Sample_contact_address | 49 Convent Dr.
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310027/suppl/GSM310027.CEL.gz
| | Sample_series_id | GSE12342
| | Sample_series_id | GSE12344
| | Sample_data_row_count | 15923
| | |
|
GSM310028 | GPL341 |
|
pineal gland, mid-night, rep 3
|
pineal gland, mid-night
|
time: mid-night
strain: Sprague-Dawley
age: 2-3 months
genotype/variation: wild type
|
in vivo pineal gland
|
| Sample_geo_accession | GSM310028
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Aug 05 2008
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Pineal glands were stored at -80oC prior to RNA extraction.
| | Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 18 hr at 45oC to an Affymetrix rat RAE230A GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL341
| | Sample_contact_name | David,C.,Klein
| | Sample_contact_email | kleind@mail.nih.gov
| | Sample_contact_phone | 3014966915
| | Sample_contact_fax | 3014803526
| | Sample_contact_laboratory | Sect. Neuroendo.
| | Sample_contact_department | PDEGEN
| | Sample_contact_institute | NICHD/NIH
| | Sample_contact_address | 49 Convent Dr.
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310028/suppl/GSM310028.CEL.gz
| | Sample_series_id | GSE12342
| | Sample_series_id | GSE12344
| | Sample_data_row_count | 15923
| | |
|
GSM310029 | GPL341 |
|
fra-2 pineal gland, mid-day, rep 1
|
DN-Fra-2 pineal gland, mid-day
|
time: mid-day
strain: Sprague-Dawley
genotype/variation: transgenic line DN-Fra-2
age: 2-3 months
|
in vivo pineal gland
|
| Sample_geo_accession | GSM310029
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Aug 05 2008
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Pineal glands were stored at -80oC prior to RNA extraction.
| | Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 18 hr at 45oC to an Affymetrix rat RAE230A GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL341
| | Sample_contact_name | David,C.,Klein
| | Sample_contact_email | kleind@mail.nih.gov
| | Sample_contact_phone | 3014966915
| | Sample_contact_fax | 3014803526
| | Sample_contact_laboratory | Sect. Neuroendo.
| | Sample_contact_department | PDEGEN
| | Sample_contact_institute | NICHD/NIH
| | Sample_contact_address | 49 Convent Dr.
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310029/suppl/GSM310029.CEL.gz
| | Sample_series_id | GSE12342
| | Sample_series_id | GSE12344
| | Sample_data_row_count | 15923
| | |
|
GSM310030 | GPL341 |
|
fra-2 pineal gland, mid-day, rep 2
|
DN-Fra-2 pineal gland, mid-day
|
time: mid-day
strain: Sprague-Dawley
genotype/variation: transgenic line DN-Fra-2
age: 2-3 months
|
in vivo pineal gland
|
| Sample_geo_accession | GSM310030
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Aug 05 2008
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Pineal glands were stored at -80oC prior to RNA extraction.
| | Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 18 hr at 45oC to an Affymetrix rat RAE230A GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL341
| | Sample_contact_name | David,C.,Klein
| | Sample_contact_email | kleind@mail.nih.gov
| | Sample_contact_phone | 3014966915
| | Sample_contact_fax | 3014803526
| | Sample_contact_laboratory | Sect. Neuroendo.
| | Sample_contact_department | PDEGEN
| | Sample_contact_institute | NICHD/NIH
| | Sample_contact_address | 49 Convent Dr.
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310030/suppl/GSM310030.CEL.gz
| | Sample_series_id | GSE12342
| | Sample_series_id | GSE12344
| | Sample_data_row_count | 15923
| | |
|
GSM310031 | GPL341 |
|
fra-2 pineal gland mid-day rep 3
|
DN-Fra-2 pineal gland, mid-day
|
time: mid-day
strain: Sprague-Dawley
genotype/variation: transgenic line DN-Fra-2
age: 2-3 months
|
in vivo pineal gland
|
| Sample_geo_accession | GSM310031
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Aug 05 2008
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Pineal glands were stored at -80oC prior to RNA extraction.
| | Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 18 hr at 45oC to an Affymetrix rat RAE230A GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL341
| | Sample_contact_name | David,C.,Klein
| | Sample_contact_email | kleind@mail.nih.gov
| | Sample_contact_phone | 3014966915
| | Sample_contact_fax | 3014803526
| | Sample_contact_laboratory | Sect. Neuroendo.
| | Sample_contact_department | PDEGEN
| | Sample_contact_institute | NICHD/NIH
| | Sample_contact_address | 49 Convent Dr.
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310031/suppl/GSM310031.CEL.gz
| | Sample_series_id | GSE12342
| | Sample_series_id | GSE12344
| | Sample_data_row_count | 15923
| | |
|
GSM310032 | GPL341 |
|
fra-2 pineal gland, mid-night, rep 1
|
DN-Fra-2 pineal gland, mid-night
|
time: mid-night
strain: Sprague-Dawley
genotype/variation: transgenic line DN-Fra-2
age: 2-3 months
|
in vivo pineal gland
|
| Sample_geo_accession | GSM310032
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Aug 05 2008
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Pineal glands were stored at -80oC prior to RNA extraction.
| | Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 18 hr at 45oC to an Affymetrix rat RAE230A GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL341
| | Sample_contact_name | David,C.,Klein
| | Sample_contact_email | kleind@mail.nih.gov
| | Sample_contact_phone | 3014966915
| | Sample_contact_fax | 3014803526
| | Sample_contact_laboratory | Sect. Neuroendo.
| | Sample_contact_department | PDEGEN
| | Sample_contact_institute | NICHD/NIH
| | Sample_contact_address | 49 Convent Dr.
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310032/suppl/GSM310032.CEL.gz
| | Sample_series_id | GSE12342
| | Sample_series_id | GSE12344
| | Sample_data_row_count | 15923
| | |
|
GSM310033 | GPL341 |
|
fra-2 pineal gland, mid-night, rep 2
|
DN-Fra-2 pineal gland, mid-night
|
time: mid-night
strain: Sprague-Dawley
genotype/variation: transgenic line DN-Fra-2
age: 2-3 months
|
in vivo pineal gland
|
| Sample_geo_accession | GSM310033
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Aug 05 2008
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Pineal glands were stored at -80oC prior to RNA extraction.
| | Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 18 hr at 45oC to an Affymetrix rat RAE230A GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL341
| | Sample_contact_name | David,C.,Klein
| | Sample_contact_email | kleind@mail.nih.gov
| | Sample_contact_phone | 3014966915
| | Sample_contact_fax | 3014803526
| | Sample_contact_laboratory | Sect. Neuroendo.
| | Sample_contact_department | PDEGEN
| | Sample_contact_institute | NICHD/NIH
| | Sample_contact_address | 49 Convent Dr.
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310033/suppl/GSM310033.CEL.gz
| | Sample_series_id | GSE12342
| | Sample_series_id | GSE12344
| | Sample_data_row_count | 15923
| | |
|
GSM310034 | GPL341 |
|
fra-2 pineal gland, mid-night, rep 3
|
DN-Fra-2 pineal gland, mid-night
|
time: mid-night
strain: Sprague-Dawley
genotype/variation: transgenic line DN-Fra-2
age: 2-3 months
|
in vivo pineal gland
|
| Sample_geo_accession | GSM310034
| | Sample_status | Public on Jan 15 2009
| | Sample_submission_date | Aug 05 2008
| | Sample_last_update_date | Apr 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Pineal glands were stored at -80oC prior to RNA extraction.
| | Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 18 hr at 45oC to an Affymetrix rat RAE230A GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL341
| | Sample_contact_name | David,C.,Klein
| | Sample_contact_email | kleind@mail.nih.gov
| | Sample_contact_phone | 3014966915
| | Sample_contact_fax | 3014803526
| | Sample_contact_laboratory | Sect. Neuroendo.
| | Sample_contact_department | PDEGEN
| | Sample_contact_institute | NICHD/NIH
| | Sample_contact_address | 49 Convent Dr.
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310034/suppl/GSM310034.CEL.gz
| | Sample_series_id | GSE12342
| | Sample_series_id | GSE12344
| | Sample_data_row_count | 15923
| | |
|
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