Search results for the GEO ID: GSE12343 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM310035 | GPL1355 |
|
pineal gland, mid-day, rep 1
|
pineal gland, mid-day
|
tissue: pineal gland
time: mid-day
strain: Sprague-Dawley
age: 2-3 months
|
in vivo pineal gland
|
Sample_geo_accession | GSM310035
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310035/suppl/GSM310035.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310036 | GPL1355 |
|
pineal gland, mid-day, rep 2
|
pineal gland, mid-day
|
tissue: pineal gland
time: mid-day
strain: Sprague-Dawley
age: 2-3 months
|
in vivo pineal gland
|
Sample_geo_accession | GSM310036
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310036/suppl/GSM310036.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310037 | GPL1355 |
|
pineal gland, mid-day, rep 3
|
pineal gland, mid-day
|
tissue: pineal gland
time: mid-day
strain: Sprague-Dawley
age: 2-3 months
|
in vivo pineal gland
|
Sample_geo_accession | GSM310037
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310037/suppl/GSM310037.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310038 | GPL1355 |
|
pineal gland, mid-day, rep 4
|
pineal gland, mid-day
|
tissue: pineal gland
time: mid-day
strain: Sprague-Dawley
age: 2-3 months
|
in vivo pineal gland
|
Sample_geo_accession | GSM310038
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310038/suppl/GSM310038.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310039 | GPL1355 |
|
pineal gland, mid-night, rep 1
|
pineal gland, mid-night
|
tissue: pineal gland
time: mid-night
strain: Sprague-Dawley
age: 2-3 months
|
in vivo pineal gland
|
Sample_geo_accession | GSM310039
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310039/suppl/GSM310039.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310040 | GPL1355 |
|
pineal gland, mid-night, rep 2
|
pineal gland, mid-night
|
tissue: pineal gland
time: mid-night
strain: Sprague-Dawley
age: 2-3 months
|
in vivo pineal gland
|
Sample_geo_accession | GSM310040
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310040/suppl/GSM310040.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310041 | GPL1355 |
|
pineal gland, mid-night, rep 3
|
pineal gland, mid-night
|
tissue: pineal gland
time: mid-night
strain: Sprague-Dawley
age: 2-3 months
|
in vivo pineal gland
|
Sample_geo_accession | GSM310041
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310041/suppl/GSM310041.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310042 | GPL1355 |
|
pineal gland, mid-night, rep 4
|
pineal gland, mid-night
|
tissue: pineal gland
time: mid-night
strain: Sprague-Dawley
age: 2-3 months
|
in vivo pineal gland
|
Sample_geo_accession | GSM310042
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310042/suppl/GSM310042.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310043 | GPL1355 |
|
retina, mid-day, rep 1
|
retina, mid-day
|
tissue: retina
time: mid-day
strain: Sprague-Dawley
age: 2-3 months
|
in vivo retina
|
Sample_geo_accession | GSM310043
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310043/suppl/GSM310043.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310044 | GPL1355 |
|
retina, mid-night, rep 2
|
retina, mid-night
|
tissue: retina
time: mid-night
strain: Sprague-Dawley
age: 2-3 months
|
in vivo retina
|
Sample_geo_accession | GSM310044
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310044/suppl/GSM310044.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310045 | GPL1355 |
|
cerebellum, mid-day, rep 1
|
cerebellum, mid-day
|
tissue: cerebellum
time: mid-day
strain: Sprague-Dawley
age: 2-3 months
|
in vivo cerebellum
|
Sample_geo_accession | GSM310045
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310045/suppl/GSM310045.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310046 | GPL1355 |
|
cerebellum, mid-night, rep 2
|
cerebellum, mid-night
|
tissue: cerebellum
time: mid-night
strain: Sprague-Dawley
age: 2-3 months
|
in vivo cerebellum
|
Sample_geo_accession | GSM310046
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310046/suppl/GSM310046.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310047 | GPL1355 |
|
cortex, mid-day, rep 1
|
cortex, mid-day
|
tissue: cortex
time: mid-day
strain: Sprague-Dawley
age: 2-3 months
|
in vivo cortex
|
Sample_geo_accession | GSM310047
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310047/suppl/GSM310047.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310048 | GPL1355 |
|
cortex, mid-night, rep 2
|
cortex, mid-night
|
tissue: cortex
time: mid-night
strain: Sprague-Dawley
age: 2-3 months
|
in vivo cortex
|
Sample_geo_accession | GSM310048
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310048/suppl/GSM310048.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310049 | GPL1355 |
|
hypothalamus, mid-day, rep 1
|
hypothalamus, mid-day
|
tissue: hypothalamus
time: mid-day
strain: Sprague-Dawley
age: 2-3 months
|
in vivo hypothalamus
|
Sample_geo_accession | GSM310049
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310049/suppl/GSM310049.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310050 | GPL1355 |
|
hypothalamus, mid-night, rep 2
|
hypothalamus, mid-night
|
tissue: hypothalamus
time: mid-night
strain: Sprague-Dawley
age: 2-3 months
|
in vivo hypothalamus
|
Sample_geo_accession | GSM310050
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310050/suppl/GSM310050.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310051 | GPL1355 |
|
liver, mid-day, rep 1
|
liver, mid-day
|
tissue: liver
time: mid-day
strain: Sprague-Dawley
age: 2-3 months
|
in vivo liver
|
Sample_geo_accession | GSM310051
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310051/suppl/GSM310051.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310052 | GPL1355 |
|
liver, mid-night, rep 2
|
liver, mid-night
|
tissue: liver
time: mid-night
strain: Sprague-Dawley
age: 2-3 months
|
in vivo liver
|
Sample_geo_accession | GSM310052
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310052/suppl/GSM310052.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310053 | GPL1355 |
|
heart, mid-day, rep 1
|
heart, mid-day
|
tissue: heart
time: mid-day
strain: Sprague-Dawley
age: 2-3 months
|
in vivo heart
|
Sample_geo_accession | GSM310053
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310053/suppl/GSM310053.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310054 | GPL1355 |
|
heart, mid-night, rep 2
|
heart, mid-night
|
tissue: heart
time: mid-night
strain: Sprague-Dawley
age: 2-3 months
|
in vivo heart
|
Sample_geo_accession | GSM310054
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310054/suppl/GSM310054.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310055 | GPL1355 |
|
cultured pineal, control, rep 1
|
cultured pineal, untreated
|
tissue: cultured pineal
agent: untreated
strain: Sprague-Dawley
age: 2-3 months
|
cultured pineal gland
|
Sample_geo_accession | GSM310055
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310055/suppl/GSM310055.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310056 | GPL1355 |
|
cultured pineal, control, rep 2
|
cultured pineal, untreated
|
tissue: cultured pineal
agent: untreated
strain: Sprague-Dawley
age: 2-3 months
|
cultured pineal gland
|
Sample_geo_accession | GSM310056
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310056/suppl/GSM310056.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310057 | GPL1355 |
|
cultured pineal, control, rep 3
|
cultured pineal, untreated
|
tissue: cultured pineal
agent: untreated
strain: Sprague-Dawley
age: 2-3 months
|
cultured pineal gland
|
Sample_geo_accession | GSM310057
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310057/suppl/GSM310057.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310058 | GPL1355 |
|
cultured pineal, norepinephrine, rep 1
|
cultured pineal, norepinephrine
|
tissue: cultured pineal
agent: norepinephrine
strain: Sprague-Dawley
age: 2-3 months
|
cultured pineal gland
|
Sample_geo_accession | GSM310058
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310058/suppl/GSM310058.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310059 | GPL1355 |
|
cultured pineal, norepinephrine, rep 2
|
cultured pineal, norepinephrine
|
tissue: cultured pineal
agent: norepinephrine
strain: Sprague-Dawley
age: 2-3 months
|
cultured pineal gland
|
Sample_geo_accession | GSM310059
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310059/suppl/GSM310059.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310060 | GPL1355 |
|
cultured pineal, norepinephrine, rep 3
|
cultured pineal, norepinephrine
|
tissue: cultured pineal
agent: norepinephrine
strain: Sprague-Dawley
age: 2-3 months
|
cultured pineal gland
|
Sample_geo_accession | GSM310060
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310060/suppl/GSM310060.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310061 | GPL1355 |
|
cultured pineal, DBcAMP, rep 1
|
cultured pineal, dibutyryl-cAMP
|
tissue: cultured pineal
agent: dibutyryl-cAMP
strain: Sprague-Dawley
age: 2-3 months
|
cultured pineal gland
|
Sample_geo_accession | GSM310061
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310061/suppl/GSM310061.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310062 | GPL1355 |
|
cultured pineal, DBcAMP, rep 2
|
cultured pineal, dibutyryl-cAMP
|
tissue: cultured pineal
agent: dibutyryl-cAMP
strain: Sprague-Dawley
age: 2-3 months
|
cultured pineal gland
|
Sample_geo_accession | GSM310062
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310062/suppl/GSM310062.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310063 | GPL1355 |
|
cultured pineal, DBcAMP, rep 3
|
cultured pineal, dibutyryl-cAMP
|
tissue: cultured pineal
agent: dibutyryl-cAMP
strain: Sprague-Dawley
age: 2-3 months
|
cultured pineal gland
|
Sample_geo_accession | GSM310063
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310063/suppl/GSM310063.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310064 | GPL1355 |
|
cultured pineal, forskolin, rep 1
|
cultured pineal, forskolin
|
tissue: cultured pineal
agent: forskolin
strain: Sprague-Dawley
age: 2-3 months
|
cultured pineal gland
|
Sample_geo_accession | GSM310064
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310064/suppl/GSM310064.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310065 | GPL1355 |
|
cultured pineal, forskolin, rep 2
|
cultured pineal, forskolin
|
tissue: cultured pineal
agent: forskolin
strain: Sprague-Dawley
age: 2-3 months
|
cultured pineal gland
|
Sample_geo_accession | GSM310065
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310065/suppl/GSM310065.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
GSM310066 | GPL1355 |
|
cultured pineal, forskolin, rep 3
|
cultured pineal, forskolin
|
tissue: cultured pineal
agent: forskolin
strain: Sprague-Dawley
age: 2-3 months
|
cultured pineal gland
|
Sample_geo_accession | GSM310066
| Sample_status | Public on Jan 15 2009
| Sample_submission_date | Aug 05 2008
| Sample_last_update_date | Apr 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Pineal glands and tissues were stored at -80oC prior to RNA extraction.
| Sample_growth_protocol_ch1 | Rats were kept on a 14 hr light:10 hr dark (14:10 LD) cycle. Pineals were taken at Zeitgeber time (ZT) 7 and ZT19. Cultured pineal glands were kept in BGJb medium for 48 hours before being treated with a vehicle (norepinephrine (1 μM), dibutyryl cAMP (0.5 or 1 mM), or forskolin (10 μM)) for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was subjected to DNase treatment using Turbo DNA-free.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45oC to an Affymetrix rat RAE230 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL1355
| Sample_contact_name | David,C.,Klein
| Sample_contact_email | kleind@mail.nih.gov
| Sample_contact_phone | 3014966915
| Sample_contact_fax | 3014803526
| Sample_contact_laboratory | Sect. Neuroendo.
| Sample_contact_department | PDEGEN
| Sample_contact_institute | NICHD/NIH
| Sample_contact_address | 49 Convent Dr.
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310066/suppl/GSM310066.CEL.gz
| Sample_series_id | GSE12343
| Sample_series_id | GSE12344
| Sample_data_row_count | 31099
| |
|
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