Result

Search results for the GEO ID: GSE12389

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM310323

GPL1261

Untreated NOD.Scid (d0)

Laser-capture microdissected islets from untreated NOD.Scid mice

Pooled from 5 untreated, adult NOD.Scid mice

Untreated NOD.Scid (d0)

GSM310419

GPL1261

Diabetogenic, d3

Laser-capture microdissected islets from NOD.Scid injected at d0 with BDC2.5 transgenic, diabetogenic T cells

pooled from 5 NOD.Scid mice induced to develop diabetes by transfer of diabetogenic T cells

Diabetogenic, d3

GSM310441

GPL1261

Protected, d3

Laser-capture microdissected islets from NOD.Scid injected at d0 with BDC2.5 transgenic, diabetogenic T cells and also injected twice with anti-Ifng antibodies

pooled from 5 NOD.Scid mice protected from developing diabetes by Ifng antibody injection

Protected, d3

Sample_geo_accession	GSM310441
Sample_status	Public on Oct 31 2008
Sample_submission_date	Aug 06 2008
Sample_last_update_date	Oct 10 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_biomaterial_provider_ch1	Emil Unanue and Jason C. Mills
Sample_treatment_protocol_ch1	Captured RNA was extracted using Agilent PicoPure columns, pooled and ~30 ng was amplified using one round of Arcturus RiboAmp HS kit amplification followed by the RNA Amplification and Labeling Kit from Enzo Life Sciences
Sample_growth_protocol_ch1	mice were sacrificed 3 days after transfer of CD4+ BDC2.5 diabetogenic T cells and following two injections of anti-Ifng antibody (at day -1 and day 2 relative to T cell transfer)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Islets were captured from frozen sections that had been fixed in methanol, briefly eosin-stained in ethanol and immediately dehydrated without exposure to aqueous solution at any time. Agilent PicoPure column with DNAse treatment was used to extract RNA from captured laser-capture caps.
Sample_label_ch1	biotin and phycoerythrin
Sample_label_protocol_ch1	Affymetrix-recommended cRNA synthesis with biotinylated nucleotides followed by fragmentation
Sample_hyb_protocol	Affymetrix standard
Sample_scan_protocol	Affymetrix standard
Sample_data_processing	dChip used to normalize and model all chips in this series
Sample_platform_id	GPL1261
Sample_contact_name	Jason,C,Mills
Sample_contact_email	jmills@wustl.edu
Sample_contact_phone	314-362-4258
Sample_contact_department	Gastroenterology, Medicine
Sample_contact_institute	Washington University
Sample_contact_address	660 S. Euclid Avenue, Box 8118
Sample_contact_city	St. Louis
Sample_contact_state	MO
Sample_contact_zip/postal_code	63110
Sample_contact_country	USA
Sample_contact_web_link	http://pathology.wustl.edu/~millslab/
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310441/suppl/GSM310441.CEL.gz
Sample_series_id	GSE12389
Sample_data_row_count	45101

GSM310442

GPL1261

Diabetogenic, d4

Laser-capture microdissected islets from NOD.Scid injected at d0 with BDC2.5 transgenic, diabetogenic T cells

pooled from 5 NOD.Scid mice after diabetogenic stimulus

Diabetogenic, d4

GSM310443

GPL1261

Protected, d4

Laser-capture microdissected islets from NOD.Scid injected at d0 with BDC2.5 transgenic, diabetogenic T cells and also injected twice with anti-Ifng antibodies

pooled from 5 NOD.Scid mice protected from developing diabetes by Ifng antibody injection

Protected, d4

Sample_geo_accession	GSM310443
Sample_status	Public on Oct 31 2008
Sample_submission_date	Aug 06 2008
Sample_last_update_date	Oct 10 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_biomaterial_provider_ch1	Emil Unanue and Jason C. Mills
Sample_treatment_protocol_ch1	Captured RNA was extracted using Agilent PicoPure columns, pooled and ~30 ng was amplified using one round of Arcturus RiboAmp HS kit amplification followed by the RNA Amplification and Labeling Kit from Enzo Life Sciences
Sample_growth_protocol_ch1	mice were sacrificed 4 days after transfer of CD4+ BDC2.5 diabetogenic T cells and following two injections of anti-Ifng antibody (at day -1 and day 2 relative to T cell transfer)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Islets were captured from frozen sections that had been fixed in methanol, briefly eosin-stained in ethanol and immediately dehydrated without exposure to aqueous solution at any time. Agilent PicoPure column with DNAse treatment was used to extract RNA from captured laser-capture caps.
Sample_label_ch1	biotin and phycoerythrin
Sample_label_protocol_ch1	Affymetrix-recommended cRNA synthesis with biotinylated nucleotides followed by fragmentation
Sample_hyb_protocol	Affymetrix standard
Sample_scan_protocol	Affymetrix standard
Sample_data_processing	dChip used to normalize and model all chips in this series
Sample_platform_id	GPL1261
Sample_contact_name	Jason,C,Mills
Sample_contact_email	jmills@wustl.edu
Sample_contact_phone	314-362-4258
Sample_contact_department	Gastroenterology, Medicine
Sample_contact_institute	Washington University
Sample_contact_address	660 S. Euclid Avenue, Box 8118
Sample_contact_city	St. Louis
Sample_contact_state	MO
Sample_contact_zip/postal_code	63110
Sample_contact_country	USA
Sample_contact_web_link	http://pathology.wustl.edu/~millslab/
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310443/suppl/GSM310443.CEL.gz
Sample_series_id	GSE12389
Sample_data_row_count	45101

GSM310444

GPL1261

Protected, d5

Laser-capture microdissected islets from NOD.Scid injected at d0 with BDC2.5 transgenic, diabetogenic T cells and also injected twice with anti-Ifng antibodies

pooled from 5 NOD.Scid mice protected from developing diabetes by Ifng antibody injection

Protected, d5

Sample_geo_accession	GSM310444
Sample_status	Public on Oct 31 2008
Sample_submission_date	Aug 06 2008
Sample_last_update_date	Oct 10 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_biomaterial_provider_ch1	Emil Unanue and Jason C. Mills
Sample_treatment_protocol_ch1	Captured RNA was extracted using Agilent PicoPure columns, pooled and ~30 ng was amplified using one round of Arcturus RiboAmp HS kit amplification followed by the RNA Amplification and Labeling Kit from Enzo Life Sciences
Sample_growth_protocol_ch1	mice were sacrificed 5 days after transfer of CD4+ BDC2.5 diabetogenic T cells and following two injections of anti-Ifng antibody (at day -1 and day 2 relative to T cell transfer)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Islets were captured from frozen sections that had been fixed in methanol, briefly eosin-stained in ethanol and immediately dehydrated without exposure to aqueous solution at any time. Agilent PicoPure column with DNAse treatment was used to extract RNA from captured laser-capture caps.
Sample_label_ch1	biotin and phycoerythrin
Sample_label_protocol_ch1	Affymetrix-recommended cRNA synthesis with biotinylated nucleotides followed by fragmentation
Sample_hyb_protocol	Affymetrix standard
Sample_scan_protocol	Affymetrix standard
Sample_data_processing	dChip used to normalize and model all chips in this series
Sample_platform_id	GPL1261
Sample_contact_name	Jason,C,Mills
Sample_contact_email	jmills@wustl.edu
Sample_contact_phone	314-362-4258
Sample_contact_department	Gastroenterology, Medicine
Sample_contact_institute	Washington University
Sample_contact_address	660 S. Euclid Avenue, Box 8118
Sample_contact_city	St. Louis
Sample_contact_state	MO
Sample_contact_zip/postal_code	63110
Sample_contact_country	USA
Sample_contact_web_link	http://pathology.wustl.edu/~millslab/
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310444/suppl/GSM310444.CEL.gz
Sample_series_id	GSE12389
Sample_data_row_count	45101

GSM310445

GPL1261

Diabetogenic, d8

Laser-capture microdissected islets from NOD.Scid injected at d0 with BDC2.5 transgenic, diabetogenic T cells

pooled from 5 NOD.Scid mice after diabetogenic stimulus

Diabetogenic, d8

GSM310446

GPL1261

Protected, d8

Laser-capture microdissected islets from NOD.Scid injected at d0 with BDC2.5 transgenic, diabetogenic T cells and also injected twice with anti-Ifng antibodies

pooled from 5 NOD.Scid mice protected from developing diabetes by Ifng antibody injection

Protected, d8

Sample_geo_accession	GSM310446
Sample_status	Public on Oct 31 2008
Sample_submission_date	Aug 06 2008
Sample_last_update_date	Oct 10 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_biomaterial_provider_ch1	Emil Unanue and Jason C. Mills
Sample_treatment_protocol_ch1	Captured RNA was extracted using Agilent PicoPure columns, pooled and ~30 ng was amplified using one round of Arcturus RiboAmp HS kit amplification followed by the RNA Amplification and Labeling Kit from Enzo Life Sciences
Sample_growth_protocol_ch1	mice were sacrificed 8 days after transfer of CD4+ BDC2.5 diabetogenic T cells and following two injections of anti-Ifng antibody (at day -1 and day 2 relative to T cell transfer)
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Islets were captured from frozen sections that had been fixed in methanol, briefly eosin-stained in ethanol and immediately dehydrated without exposure to aqueous solution at any time. Agilent PicoPure column with DNAse treatment was used to extract RNA from captured laser-capture caps.
Sample_label_ch1	biotin and phycoerythrin
Sample_label_protocol_ch1	Affymetrix-recommended cRNA synthesis with biotinylated nucleotides followed by fragmentation
Sample_hyb_protocol	Affymetrix standard
Sample_scan_protocol	Affymetrix standard
Sample_data_processing	dChip used to normalize and model all chips in this series
Sample_platform_id	GPL1261
Sample_contact_name	Jason,C,Mills
Sample_contact_email	jmills@wustl.edu
Sample_contact_phone	314-362-4258
Sample_contact_department	Gastroenterology, Medicine
Sample_contact_institute	Washington University
Sample_contact_address	660 S. Euclid Avenue, Box 8118
Sample_contact_city	St. Louis
Sample_contact_state	MO
Sample_contact_zip/postal_code	63110
Sample_contact_country	USA
Sample_contact_web_link	http://pathology.wustl.edu/~millslab/
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM310nnn/GSM310446/suppl/GSM310446.CEL.gz
Sample_series_id	GSE12389
Sample_data_row_count	45101

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