Search results for the GEO ID: GSE12444  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM312556 | GPL570 |
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PrSC, control siRNA, array C
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Human primary prostate stromal cells, negative control siRNA
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Control primary prostate stromal cells.
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Gene expression of PrSC cells treated with negative control siRNA.
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| Sample_geo_accession | GSM312556
| | Sample_status | Public on Nov 01 2008
| | Sample_submission_date | Aug 14 2008
| | Sample_last_update_date | Sep 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Incubation with negative control siRNA, 72h, special PRSC medium without serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated from the prostate stromal cells by lysing the cells (PCU, Kinematica GmbH, Luzern, Switzerland) in RNAbee reagent (Tel-test Inc, Friendswood, USA). Chloroform was added and centrifuged at 12,000 g. The water phase was extracted and precipitated with isopropanol. The RNA was washed with 75 % ethanol and dissolved in RNAse free water.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 5ug of total RNA was used to prepare antisense biotinylated RNA according to manufacturer's one-cycle instructions (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Hybridization to Affymetrix Human U133plus2.0 GeneChips (54,614 probe sets, representing approximately 47,000 transcripts), staining, and washing were performed as described by Affymetrix (Affymetrix, Santa Clara, CA, USA) and performed by Erasmus MC Center for Biomics.
| | Sample_scan_protocol | Scanning procedures were performed as described by Affymetrix (Affymetrix, Santa Clara, CA, USA) and performed by Erasmus MC Center for Biomics.
| | Sample_data_processing | Data was processed using Affymetrix GCOS software (MAS5.0 algorithm).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leonie,,van der Heul
| | Sample_contact_institute | Erasmus MC
| | Sample_contact_address | Dr. Molewaterplein 50
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3015 GE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312556/suppl/GSM312556.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312556/suppl/GSM312556.CHP.gz
| | Sample_series_id | GSE12444
| | Sample_data_row_count | 54675
| | |
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GSM312558 | GPL570 |
|
PrSC, FOXF2 siRNA, array A
|
Human primary prostate stromal cells, siRNA directed against FOXF2
|
Primary prostate stromal cells in which FOXF2 is knocked down.
|
Gene expression of PrSC cells treated with FOXF2 siRNA.
|
| Sample_geo_accession | GSM312558
| | Sample_status | Public on Nov 01 2008
| | Sample_submission_date | Aug 14 2008
| | Sample_last_update_date | Sep 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Incubation with siRNA directed against FOXF2, 72h, special PRSC medium without serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated from the prostate stromal cells by lysing the cells (PCU, Kinematica GmbH, Luzern, Switzerland) in RNAbee reagent (Tel-test Inc, Friendswood, USA). Chloroform was added and centrifuged at 12,000 g. The water phase was extracted and precipitated with isopropanol. The RNA was washed with 75 % ethanol and dissolved in RNAse free water.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 5ug of total RNA was used to prepare antisense biotinylated RNA according to manufacturer's one-cycle instructions (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Hybridization to Affymetrix Human U133plus2.0 GeneChips (54,614 probe sets, representing approximately 47,000 transcripts), staining, and washing were performed as described by Affymetrix (Affymetrix, Santa Clara, CA, USA) and performed by Erasmus MC Center for Biomics.
| | Sample_scan_protocol | Scanning procedures were performed as described by Affymetrix (Affymetrix, Santa Clara, CA, USA) and performed by Erasmus MC Center for Biomics.
| | Sample_data_processing | Data was processed using Affymetrix GCOS software (MAS5.0 algorithm).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leonie,,van der Heul
| | Sample_contact_institute | Erasmus MC
| | Sample_contact_address | Dr. Molewaterplein 50
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3015 GE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312558/suppl/GSM312558.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312558/suppl/GSM312558.CHP.gz
| | Sample_series_id | GSE12444
| | Sample_data_row_count | 54675
| | |
|
GSM312562 | GPL570 |
|
PrSC, control siRNA, array A
|
Human primary prostate stromal cells, negative control siRNA
|
Control primary prostate stromal cells.
|
Gene expression of PrSC cells treated with negative control siRNA.
|
| Sample_geo_accession | GSM312562
| | Sample_status | Public on Nov 01 2008
| | Sample_submission_date | Aug 14 2008
| | Sample_last_update_date | Sep 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Incubation with negative control siRNA, 72h, special PRSC medium without serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated from the prostate stromal cells by lysing the cells (PCU, Kinematica GmbH, Luzern, Switzerland) in RNAbee reagent (Tel-test Inc, Friendswood, USA). Chloroform was added and centrifuged at 12,000 g. The water phase was extracted and precipitated with isopropanol. The RNA was washed with 75 % ethanol and dissolved in RNAse free water.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 5ug of total RNA was used to prepare antisense biotinylated RNA according to manufacturer's one-cycle instructions (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Hybridization to Affymetrix Human U133plus2.0 GeneChips (54,614 probe sets, representing approximately 47,000 transcripts), staining, and washing were performed as described by Affymetrix (Affymetrix, Santa Clara, CA, USA) and performed by Erasmus MC Center for Biomics.
| | Sample_scan_protocol | Scanning procedures were performed as described by Affymetrix (Affymetrix, Santa Clara, CA, USA) and performed by Erasmus MC Center for Biomics.
| | Sample_data_processing | Data was processed using Affymetrix GCOS software (MAS5.0 algorithm).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leonie,,van der Heul
| | Sample_contact_institute | Erasmus MC
| | Sample_contact_address | Dr. Molewaterplein 50
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3015 GE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312562/suppl/GSM312562.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312562/suppl/GSM312562.CHP.gz
| | Sample_series_id | GSE12444
| | Sample_data_row_count | 54675
| | |
|
GSM312670 | GPL570 |
|
PrSC, FOXF2 siRNA, array B
|
Human primary prostate stromal cells, siRNA directed against FOXF2
|
Primary prostate stromal cells in which FOXF2 is knocked down.
|
Gene expression of PrSC cells treated with FOXF2 siRNA.
|
| Sample_geo_accession | GSM312670
| | Sample_status | Public on Nov 01 2008
| | Sample_submission_date | Aug 14 2008
| | Sample_last_update_date | Sep 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Incubation with siRNA directed against FOXF2, 72h, special PRSC medium without serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated from the prostate stromal cells by lysing the cells (PCU, Kinematica GmbH, Luzern, Switzerland) in RNAbee reagent (Tel-test Inc, Friendswood, USA). Chloroform was added and centrifuged at 12,000 g. The water phase was extracted and precipitated with isopropanol. The RNA was washed with 75 % ethanol and dissolved in RNAse free water.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 5ug of total RNA was used to prepare antisense biotinylated RNA according to manufacturer's one-cycle instructions (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Hybridization to Affymetrix Human U133plus2.0 GeneChips (54,614 probe sets, representing approximately 47,000 transcripts), staining, and washing were performed as described by Affymetrix (Affymetrix, Santa Clara, CA, USA) and performed by Erasmus MC Center for Biomics.
| | Sample_scan_protocol | Scanning procedures were performed as described by Affymetrix (Affymetrix, Santa Clara, CA, USA) and performed by Erasmus MC Center for Biomics.
| | Sample_data_processing | Data was processed using Affymetrix GCOS software (MAS5.0 algorithm).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leonie,,van der Heul
| | Sample_contact_institute | Erasmus MC
| | Sample_contact_address | Dr. Molewaterplein 50
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3015 GE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312670/suppl/GSM312670.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312670/suppl/GSM312670.CHP.gz
| | Sample_series_id | GSE12444
| | Sample_data_row_count | 54675
| | |
|
GSM312672 | GPL570 |
|
PrSC, control siRNA, array B
|
Human primary prostate stromal cells, negative control siRNA
|
Control primary prostate stromal cells.
|
Gene expression of PrSC cells treated with negative control siRNA.
|
| Sample_geo_accession | GSM312672
| | Sample_status | Public on Nov 01 2008
| | Sample_submission_date | Aug 14 2008
| | Sample_last_update_date | Sep 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Incubation with negative control siRNA, 72h, special PRSC medium without serum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated from the prostate stromal cells by lysing the cells (PCU, Kinematica GmbH, Luzern, Switzerland) in RNAbee reagent (Tel-test Inc, Friendswood, USA). Chloroform was added and centrifuged at 12,000 g. The water phase was extracted and precipitated with isopropanol. The RNA was washed with 75 % ethanol and dissolved in RNAse free water.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 5ug of total RNA was used to prepare antisense biotinylated RNA according to manufacturer's one-cycle instructions (Affymetrix, Santa Clara, CA, USA).
| | Sample_hyb_protocol | Hybridization to Affymetrix Human U133plus2.0 GeneChips (54,614 probe sets, representing approximately 47,000 transcripts), staining, and washing were performed as described by Affymetrix (Affymetrix, Santa Clara, CA, USA) and performed by Erasmus MC Center for Biomics.
| | Sample_scan_protocol | Scanning procedures were performed as described by Affymetrix (Affymetrix, Santa Clara, CA, USA) and performed by Erasmus MC Center for Biomics.
| | Sample_data_processing | Data was processed using Affymetrix GCOS software (MAS5.0 algorithm).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leonie,,van der Heul
| | Sample_contact_institute | Erasmus MC
| | Sample_contact_address | Dr. Molewaterplein 50
| | Sample_contact_city | Rotterdam
| | Sample_contact_zip/postal_code | 3015 GE
| | Sample_contact_country | Netherlands
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312672/suppl/GSM312672.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312672/suppl/GSM312672.CHP.gz
| | Sample_series_id | GSE12444
| | Sample_data_row_count | 54675
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