Search results for the GEO ID: GSE12488  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM312774 | GPL570 |
|
Case 1Sp, patient with TCRVbeta13.1+/CD4+/Nka+/CD8-/+ dim T-LGL lymphocytosis
|
purified CD69+ hCMV-stimulated monoclonal CD4+ T-LGL from human peripheral blood
|
Patient identifier: 15148 CMV 69+
Sex: male
Cell purity of ≥ 98%
purified CD69+ hCMV-stimulated monoclonal CD4+ T-LGL from peripheral blood of male patient with TCRVbeta13.1+/CD4+ T-LGL lymphocytosis
|
purified CD69+ hCMV-stimulated monoclonal CD4+ T-LGL from peripheral blood of male patient with TCRVbeta13.1+/CD4+ T-LGL lymphocytosis
|
| Sample_geo_accession | GSM312774
| | Sample_status | Public on Sep 01 2008
| | Sample_submission_date | Aug 14 2008
| | Sample_last_update_date | Aug 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from magnetic-activated cell sorter (MACS)-freshly purified unstimulated monoclonal CD4+ T-LGL lymphocytes from PB sample (purity of ≥98%). To get pure and highly concentrated RNA, the silica membrane technology NucleoSpin® RNA XS (Macherey-Nagel, Düren, Germany) was used.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total RNA from the purified cell fractions was amplified and labeled using the GeneChip two cycle cDNA synthesis kit and the GeneChip IVT labeling kit (Affymetrix Inc., Santa Clara, CA), respectively.
| | Sample_hyb_protocol | Following fragmentation, 25 microg of cRNA were hybridized to the HG-U133 Plus 2.0 Array chip (Affymetrix) according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | RNA expression values for each probe set were calculated using the RMA (“Robust Multi-array Average”) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Arancha,,Rodríguez-Caballero
| | Sample_contact_email | arocab@usal.es
| | Sample_contact_institute | Cancer Research Center. University of Salamanca.
| | Sample_contact_address | Avda. Universidad de Coimbra. Campus Unamuno
| | Sample_contact_city | Salamanca
| | Sample_contact_zip/postal_code | 37007
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312774/suppl/GSM312774.CEL.gz
| | Sample_series_id | GSE12488
| | Sample_data_row_count | 54675
| | |
|
GSM312775 | GPL570 |
|
Case 1Sn, patient with TCRVbeta13.1+/CD4+/Nka+/CD8-/+ dim T-LGL lymphocytosis
|
purified CD69- hCMV-stimulated monoclonal CD4+ T-LGL from human peripheral blood
|
Patient identifier: 15148 CMV 56+ 69-
Sex: male
Cell purity of ≥ 98%
purified CD69- hCMV-stimulated monoclonal CD4+ T-LGL from peripheral blood of male patient with TCRVbeta13.1+/CD4+ T-LGL lymphocytosis
|
purified CD69- hCMV-stimulated monoclonal CD4+ T-LGL from peripheral blood of male patient with TCRVbeta13.1+/CD4+ T-LGL lymphocytosis
|
| Sample_geo_accession | GSM312775
| | Sample_status | Public on Sep 01 2008
| | Sample_submission_date | Aug 14 2008
| | Sample_last_update_date | Aug 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from magnetic-activated cell sorter (MACS)-freshly purified unstimulated monoclonal CD4+ T-LGL lymphocytes from PB sample (purity of ≥98%). To get pure and highly concentrated RNA, the silica membrane technology NucleoSpin® RNA XS (Macherey-Nagel, Düren, Germany) was used.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total RNA from the purified cell fractions was amplified and labeled using the GeneChip two cycle cDNA synthesis kit and the GeneChip IVT labeling kit (Affymetrix Inc., Santa Clara, CA), respectively.
| | Sample_hyb_protocol | Following fragmentation, 25 microg of cRNA were hybridized to the HG-U133 Plus 2.0 Array chip (Affymetrix) according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | RNA expression values for each probe set were calculated using the RMA (“Robust Multi-array Average”) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Arancha,,Rodríguez-Caballero
| | Sample_contact_email | arocab@usal.es
| | Sample_contact_institute | Cancer Research Center. University of Salamanca.
| | Sample_contact_address | Avda. Universidad de Coimbra. Campus Unamuno
| | Sample_contact_city | Salamanca
| | Sample_contact_zip/postal_code | 37007
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312775/suppl/GSM312775.CEL.gz
| | Sample_series_id | GSE12488
| | Sample_data_row_count | 54675
| | |
|
GSM312776 | GPL570 |
|
Case 2F, patient with TCRVbeta13.1+/CD4+/Nka+/CD8-/+ dim T-LGL lymphocytosis
|
Freshly isolated monoclonal CD4+ T-LGL from human peripheral blood
|
Patient identifier: 15163
Sex: Female
Cell purity of ≥ 98%
Freshly isolated monoclonal CD4+ T-LGL from peripheral blood of female patient with TCRVbeta13.1+/CD4+ T-LGL lymphocytosis
|
Freshly isolated monoclonal CD4+ T-LGL from peripheral blood of female patient with TCRVbeta13.1+/CD4+ T-LGL lymphocytosis
|
| Sample_geo_accession | GSM312776
| | Sample_status | Public on Sep 01 2008
| | Sample_submission_date | Aug 14 2008
| | Sample_last_update_date | Aug 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from magnetic-activated cell sorter (MACS)-freshly purified unstimulated monoclonal CD4+ T-LGL lymphocytes from PB sample (purity of ≥98%). To get pure and highly concentrated RNA, the silica membrane technology NucleoSpin® RNA XS (Macherey-Nagel, Düren, Germany) was used.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total RNA from the purified cell fractions was amplified and labeled using the GeneChip two cycle cDNA synthesis kit and the GeneChip IVT labeling kit (Affymetrix Inc., Santa Clara, CA), respectively.
| | Sample_hyb_protocol | Following fragmentation, 25 microg of cRNA were hybridized to the HG-U133 Plus 2.0 Array chip (Affymetrix) according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | RNA expression values for each probe set were calculated using the RMA (“Robust Multi-array Average”) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Arancha,,Rodríguez-Caballero
| | Sample_contact_email | arocab@usal.es
| | Sample_contact_institute | Cancer Research Center. University of Salamanca.
| | Sample_contact_address | Avda. Universidad de Coimbra. Campus Unamuno
| | Sample_contact_city | Salamanca
| | Sample_contact_zip/postal_code | 37007
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312776/suppl/GSM312776.CEL.gz
| | Sample_series_id | GSE12488
| | Sample_data_row_count | 54675
| | |
|
GSM312777 | GPL570 |
|
Case 2Sp, patient with TCRVbeta13.1+/CD4+/Nka+/CD8-/+ dim T-LGL lymphocytosis
|
purified CD69+ hCMV-stimulated monoclonal CD4+ T-LGL from human peripheral blood
|
Patient identifier: 15163 CMV 69+
Sex: Female
Cell purity of ≥ 98%
purified CD69+ hCMV-stimulated monoclonal CD4+ T-LGL from peripheral blood of female patient with TCRVbeta13.1+/CD4+ T-LGL lymphocytosis
|
purified CD69+ hCMV-stimulated monoclonal CD4+ T-LGL from peripheral blood of female patient with TCRVbeta13.1+/CD4+ T-LGL lymphocytosis
|
| Sample_geo_accession | GSM312777
| | Sample_status | Public on Sep 01 2008
| | Sample_submission_date | Aug 14 2008
| | Sample_last_update_date | Aug 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from magnetic-activated cell sorter (MACS)-freshly purified unstimulated monoclonal CD4+ T-LGL lymphocytes from PB sample (purity of ≥98%). To get pure and highly concentrated RNA, the silica membrane technology NucleoSpin® RNA XS (Macherey-Nagel, Düren, Germany) was used.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total RNA from the purified cell fractions was amplified and labeled using the GeneChip two cycle cDNA synthesis kit and the GeneChip IVT labeling kit (Affymetrix Inc., Santa Clara, CA), respectively.
| | Sample_hyb_protocol | Following fragmentation, 25 microg of cRNA were hybridized to the HG-U133 Plus 2.0 Array chip (Affymetrix) according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | RNA expression values for each probe set were calculated using the RMA (“Robust Multi-array Average”) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Arancha,,Rodríguez-Caballero
| | Sample_contact_email | arocab@usal.es
| | Sample_contact_institute | Cancer Research Center. University of Salamanca.
| | Sample_contact_address | Avda. Universidad de Coimbra. Campus Unamuno
| | Sample_contact_city | Salamanca
| | Sample_contact_zip/postal_code | 37007
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312777/suppl/GSM312777.CEL.gz
| | Sample_series_id | GSE12488
| | Sample_data_row_count | 54675
| | |
|
GSM312778 | GPL570 |
|
Case 2Sn, patient with TCRVbeta13.1+/CD4+/Nka+/CD8-/+ dim T-LGL lymphocytosis
|
purified CD69- hCMV-stimulated monoclonal CD4+ T-LGL from human peripheral blood
|
Patient identifier: 15163 CMV CD4+ 69-
Sex: Female
Cell purity of ≥ 98%
purified CD69- hCMV-stimulated monoclonal CD4+ T-LGL from peripheral blood of female patient with TCRVbeta13.1+/CD4+ T-LGL lymphocytosis
|
purified CD69- hCMV-stimulated monoclonal CD4+ T-LGL from peripheral blood of female patient with TCRVbeta13.1+/CD4+ T-LGL lymphocytosis
|
| Sample_geo_accession | GSM312778
| | Sample_status | Public on Sep 01 2008
| | Sample_submission_date | Aug 14 2008
| | Sample_last_update_date | Aug 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from magnetic-activated cell sorter (MACS)-freshly purified unstimulated monoclonal CD4+ T-LGL lymphocytes from PB sample (purity of ≥98%). To get pure and highly concentrated RNA, the silica membrane technology NucleoSpin® RNA XS (Macherey-Nagel, Düren, Germany) was used.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total RNA from the purified cell fractions was amplified and labeled using the GeneChip two cycle cDNA synthesis kit and the GeneChip IVT labeling kit (Affymetrix Inc., Santa Clara, CA), respectively.
| | Sample_hyb_protocol | Following fragmentation, 25 microg of cRNA were hybridized to the HG-U133 Plus 2.0 Array chip (Affymetrix) according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | RNA expression values for each probe set were calculated using the RMA (“Robust Multi-array Average”) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Arancha,,Rodríguez-Caballero
| | Sample_contact_email | arocab@usal.es
| | Sample_contact_institute | Cancer Research Center. University of Salamanca.
| | Sample_contact_address | Avda. Universidad de Coimbra. Campus Unamuno
| | Sample_contact_city | Salamanca
| | Sample_contact_zip/postal_code | 37007
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312778/suppl/GSM312778.CEL.gz
| | Sample_series_id | GSE12488
| | Sample_data_row_count | 54675
| | |
|
GSM312779 | GPL570 |
|
Case 3F, patient with TCRVbeta11+/CD4+/Nka+/CD8-/+ dim T-LGL lymphocytosis
|
Freshly isolated monoclonal CD4+ T-LGL from human peripheral blood
|
Patient identifier: 13364 FRAC+2
Sex: male
Cell purity of ≥ 98%
Freshly isolated monoclonal CD4+ T-LGL from peripheral blood of male patient with TCRVbeta11+/CD4+ T-LGL lymphocytosis
|
Freshly isolated monoclonal CD4+ T-LGL from peripheral blood of male patient with TCRVbeta11+/CD4+ T-LGL lymphocytosis
|
| Sample_geo_accession | GSM312779
| | Sample_status | Public on Sep 01 2008
| | Sample_submission_date | Aug 14 2008
| | Sample_last_update_date | Aug 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from magnetic-activated cell sorter (MACS)-freshly purified unstimulated monoclonal CD4+ T-LGL lymphocytes from PB sample (purity of ≥98%). To get pure and highly concentrated RNA, the silica membrane technology NucleoSpin® RNA XS (Macherey-Nagel, Düren, Germany) was used.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total RNA from the purified cell fractions was amplified and labeled using the GeneChip two cycle cDNA synthesis kit and the GeneChip IVT labeling kit (Affymetrix Inc., Santa Clara, CA), respectively.
| | Sample_hyb_protocol | Following fragmentation, 25 microg of cRNA were hybridized to the HG-U133 Plus 2.0 Array chip (Affymetrix) according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | RNA expression values for each probe set were calculated using the RMA (“Robust Multi-array Average”) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Arancha,,Rodríguez-Caballero
| | Sample_contact_email | arocab@usal.es
| | Sample_contact_institute | Cancer Research Center. University of Salamanca.
| | Sample_contact_address | Avda. Universidad de Coimbra. Campus Unamuno
| | Sample_contact_city | Salamanca
| | Sample_contact_zip/postal_code | 37007
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312779/suppl/GSM312779.CEL.gz
| | Sample_series_id | GSE12488
| | Sample_data_row_count | 54675
| | |
|
GSM312781 | GPL570 |
|
Case 3Sp, patient with TCRVbeta11+/CD4+/Nka+/CD8-/+ dim T-LGL lymphocytosis
|
purified CD69+ hCMV-stimulated monoclonal CD4+ T-LGL from human peripheral blood
|
Patient identifier: 13364 CMV 69+
Sex: male
Cell purity of ≥ 98%
purified CD69+ hCMV-stimulated monoclonal CD4+ T-LGL from peripheral blood of male patient with TCRVbeta11+/CD4+ T-LGL lymphocytosis
|
purified CD69+ hCMV-stimulated monoclonal CD4+ T-LGL from peripheral blood of male patient with TCRVbeta11+/CD4+ T-LGL lymphocytosis
|
| Sample_geo_accession | GSM312781
| | Sample_status | Public on Sep 01 2008
| | Sample_submission_date | Aug 14 2008
| | Sample_last_update_date | Aug 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from magnetic-activated cell sorter (MACS)-freshly purified unstimulated monoclonal CD4+ T-LGL lymphocytes from PB sample (purity of ≥98%). To get pure and highly concentrated RNA, the silica membrane technology NucleoSpin® RNA XS (Macherey-Nagel, Düren, Germany) was used.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total RNA from the purified cell fractions was amplified and labeled using the GeneChip two cycle cDNA synthesis kit and the GeneChip IVT labeling kit (Affymetrix Inc., Santa Clara, CA), respectively.
| | Sample_hyb_protocol | Following fragmentation, 25 microg of cRNA were hybridized to the HG-U133 Plus 2.0 Array chip (Affymetrix) according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | RNA expression values for each probe set were calculated using the RMA (“Robust Multi-array Average”) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Arancha,,Rodríguez-Caballero
| | Sample_contact_email | arocab@usal.es
| | Sample_contact_institute | Cancer Research Center. University of Salamanca.
| | Sample_contact_address | Avda. Universidad de Coimbra. Campus Unamuno
| | Sample_contact_city | Salamanca
| | Sample_contact_zip/postal_code | 37007
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312781/suppl/GSM312781.CEL.gz
| | Sample_series_id | GSE12488
| | Sample_data_row_count | 54675
| | |
|
GSM312782 | GPL570 |
|
Case 3Sn, patient with TCRVbeta11+/CD4+/Nka+/CD8-/+ dim T-LGL lymphocytosis
|
purified CD69- hCMV-stimulated monoclonal CD4+ T-LGL from human peripheral blood
|
Patient identifier: 13364 CMV 56+ 69-
Sex: male
Cell purity of ≥ 98%
purified CD69- hCMV-stimulated monoclonal CD4+ T-LGL from peripheral blood of male patient with TCRVbeta11+/CD4+ T-LGL lymphocytosis
|
purified CD69- hCMV-stimulated monoclonal CD4+ T-LGL from peripheral blood of male patient with TCRVbeta11+/CD4+ T-LGL lymphocytosis
|
| Sample_geo_accession | GSM312782
| | Sample_status | Public on Sep 01 2008
| | Sample_submission_date | Aug 14 2008
| | Sample_last_update_date | Aug 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from magnetic-activated cell sorter (MACS)-freshly purified unstimulated monoclonal CD4+ T-LGL lymphocytes from PB sample (purity of ≥98%). To get pure and highly concentrated RNA, the silica membrane technology NucleoSpin® RNA XS (Macherey-Nagel, Düren, Germany) was used.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total RNA from the purified cell fractions was amplified and labeled using the GeneChip two cycle cDNA synthesis kit and the GeneChip IVT labeling kit (Affymetrix Inc., Santa Clara, CA), respectively.
| | Sample_hyb_protocol | Following fragmentation, 25 microg of cRNA were hybridized to the HG-U133 Plus 2.0 Array chip (Affymetrix) according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | RNA expression values for each probe set were calculated using the RMA (“Robust Multi-array Average”) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Arancha,,Rodríguez-Caballero
| | Sample_contact_email | arocab@usal.es
| | Sample_contact_institute | Cancer Research Center. University of Salamanca.
| | Sample_contact_address | Avda. Universidad de Coimbra. Campus Unamuno
| | Sample_contact_city | Salamanca
| | Sample_contact_zip/postal_code | 37007
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312782/suppl/GSM312782.CEL.gz
| | Sample_series_id | GSE12488
| | Sample_data_row_count | 54675
| | |
|
GSM312783 | GPL570 |
|
Case 4F, patient with TCRVbeta13.1+/CD4+/Nka+/CD8-/+ dim T-LGL lymphocytosis
|
Freshly isolated monoclonal CD4+ T-LGL from human peripheral blood
|
Patient identifier: 15718 FRAC+ 56+
Sex: Female
Cell purity of ≥ 98%
Freshly isolated monoclonal CD4+ T-LGL from peripheral blood of female patient with TCRVbeta13.1+/CD4+ T-LGL lymphocytosis
|
Freshly isolated monoclonal CD4+ T-LGL from peripheral blood of female patient with TCRVbeta13.1+/CD4+ T-LGL lymphocytosis
|
| Sample_geo_accession | GSM312783
| | Sample_status | Public on Sep 01 2008
| | Sample_submission_date | Aug 14 2008
| | Sample_last_update_date | Aug 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from magnetic-activated cell sorter (MACS)-freshly purified unstimulated monoclonal CD4+ T-LGL lymphocytes from PB sample (purity of ≥98%). To get pure and highly concentrated RNA, the silica membrane technology NucleoSpin® RNA XS (Macherey-Nagel, Düren, Germany) was used.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total RNA from the purified cell fractions was amplified and labeled using the GeneChip two cycle cDNA synthesis kit and the GeneChip IVT labeling kit (Affymetrix Inc., Santa Clara, CA), respectively.
| | Sample_hyb_protocol | Following fragmentation, 25 microg of cRNA were hybridized to the HG-U133 Plus 2.0 Array chip (Affymetrix) according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | RNA expression values for each probe set were calculated using the RMA (“Robust Multi-array Average”) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Arancha,,Rodríguez-Caballero
| | Sample_contact_email | arocab@usal.es
| | Sample_contact_institute | Cancer Research Center. University of Salamanca.
| | Sample_contact_address | Avda. Universidad de Coimbra. Campus Unamuno
| | Sample_contact_city | Salamanca
| | Sample_contact_zip/postal_code | 37007
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312783/suppl/GSM312783.CEL.gz
| | Sample_series_id | GSE12488
| | Sample_data_row_count | 54675
| | |
|
GSM312784 | GPL570 |
|
Case 4Sp, patient with TCRVbeta13.1+/CD4+/Nka+/CD8-/+ dim T-LGL lymphocytosis
|
purified CD69+ hCMV-stimulated monoclonal CD4+ T-LGL from human peripheral blood
|
Patient identifier: 15109 CMV 69+
Sex: Female
Cell purity of ≥ 98%
purified CD69+ hCMV-stimulated monoclonal CD4+ T-LGL from peripheral blood of female patient with TCRVbeta13.1+/CD4+ T-LGL lymphocytosis
|
purified CD69+ hCMV-stimulated monoclonal CD4+ T-LGL from peripheral blood of female patient with TCRVbeta13.1+/CD4+ T-LGL lymphocytosis
|
| Sample_geo_accession | GSM312784
| | Sample_status | Public on Sep 01 2008
| | Sample_submission_date | Aug 14 2008
| | Sample_last_update_date | Aug 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from magnetic-activated cell sorter (MACS)-freshly purified unstimulated monoclonal CD4+ T-LGL lymphocytes from PB sample (purity of ≥98%). To get pure and highly concentrated RNA, the silica membrane technology NucleoSpin® RNA XS (Macherey-Nagel, Düren, Germany) was used.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total RNA from the purified cell fractions was amplified and labeled using the GeneChip two cycle cDNA synthesis kit and the GeneChip IVT labeling kit (Affymetrix Inc., Santa Clara, CA), respectively.
| | Sample_hyb_protocol | Following fragmentation, 25 microg of cRNA were hybridized to the HG-U133 Plus 2.0 Array chip (Affymetrix) according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | RNA expression values for each probe set were calculated using the RMA (“Robust Multi-array Average”) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Arancha,,Rodríguez-Caballero
| | Sample_contact_email | arocab@usal.es
| | Sample_contact_institute | Cancer Research Center. University of Salamanca.
| | Sample_contact_address | Avda. Universidad de Coimbra. Campus Unamuno
| | Sample_contact_city | Salamanca
| | Sample_contact_zip/postal_code | 37007
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312784/suppl/GSM312784.CEL.gz
| | Sample_series_id | GSE12488
| | Sample_data_row_count | 54675
| | |
|
GSM312786 | GPL570 |
|
Case 4Sn, patient with TCRVbeta13.1+/CD4+/Nka+/CD8-/+ dim T-LGL lymphocytosis
|
purified CD69- hCMV-stimulated monoclonal CD4+ T-LGL from human peripheral blood
|
Patient identifier: 15718 CMV 69- 56+
Sex: Female
Cell purity of ≥ 98%
purified CD69- hCMV-stimulated monoclonal CD4+ T-LGL from peripheral blood of female patient with TCRVbeta13.1+/CD4+ T-LGL lymphocytosis
|
purified CD69- hCMV-stimulated monoclonal CD4+ T-LGL from peripheral blood of female patient with TCRVbeta13.1+/CD4+ T-LGL lymphocytosis
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| Sample_geo_accession | GSM312786
| | Sample_status | Public on Sep 01 2008
| | Sample_submission_date | Aug 14 2008
| | Sample_last_update_date | Aug 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from magnetic-activated cell sorter (MACS)-freshly purified unstimulated monoclonal CD4+ T-LGL lymphocytes from PB sample (purity of ≥98%). To get pure and highly concentrated RNA, the silica membrane technology NucleoSpin® RNA XS (Macherey-Nagel, Düren, Germany) was used.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total RNA from the purified cell fractions was amplified and labeled using the GeneChip two cycle cDNA synthesis kit and the GeneChip IVT labeling kit (Affymetrix Inc., Santa Clara, CA), respectively.
| | Sample_hyb_protocol | Following fragmentation, 25 microg of cRNA were hybridized to the HG-U133 Plus 2.0 Array chip (Affymetrix) according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | RNA expression values for each probe set were calculated using the RMA (“Robust Multi-array Average”) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Arancha,,Rodríguez-Caballero
| | Sample_contact_email | arocab@usal.es
| | Sample_contact_institute | Cancer Research Center. University of Salamanca.
| | Sample_contact_address | Avda. Universidad de Coimbra. Campus Unamuno
| | Sample_contact_city | Salamanca
| | Sample_contact_zip/postal_code | 37007
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312786/suppl/GSM312786.CEL.gz
| | Sample_series_id | GSE12488
| | Sample_data_row_count | 54675
| | |
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GSM313672 | GPL570 |
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Donor1, hCMV seropositive healthy donor
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hCMV-stimulated (specific) CD69+ CD4+ T-lymphocytes isolated from human peripheral blood
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Donor identifier: Jose Mo
Sex: male
Cell purity of 98%
hCMV-stimulated (specific) CD69+ CD4+ T-lymphocytes isolated from human peripheral blood of male hCMV-seropositive healthy donor
|
hCMV-stimulated (specific) CD69+ CD4+ T-lymphocytes isolated from human peripheral blood of male hCMV-seropositive healthy donor
|
| Sample_geo_accession | GSM313672
| | Sample_status | Public on Sep 01 2008
| | Sample_submission_date | Aug 19 2008
| | Sample_last_update_date | Aug 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the silica membrane technology NucleoSpinRNA XS (Macherey-Nagel, Düren, Germany)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total RNA from the purified cell fractions was amplified and labeled using the GeneChip two cycle cDNA synthesis kit and the GeneChip IVT labeling kit (Affymetrix Inc., Santa Clara, CA), respectively.
| | Sample_hyb_protocol | Following fragmentation, 25 microg of cRNA were hybridized to the HG-U133 Plus 2.0 Array chip (Affymetrix) according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | RNA expression values for each probe set were calculated using the RMA (“Robust Multi-array Average”) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Arancha,,Rodríguez-Caballero
| | Sample_contact_email | arocab@usal.es
| | Sample_contact_institute | Cancer Research Center. University of Salamanca.
| | Sample_contact_address | Avda. Universidad de Coimbra. Campus Unamuno
| | Sample_contact_city | Salamanca
| | Sample_contact_zip/postal_code | 37007
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM313nnn/GSM313672/suppl/GSM313672.CEL.gz
| | Sample_series_id | GSE12488
| | Sample_data_row_count | 54675
| | |
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GSM313675 | GPL570 |
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Donor2, hCMV seropositive healthy donor
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hCMV-stimulated (specific) CD69+ CD4+ T-lymphocytes isolated from human peripheral blood
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Donor identifier: Anc1
Sex: male
Cell purity of 98%
hCMV-stimulated (specific) CD69+ CD4+ T-lymphocytes isolated from human peripheral blood of male hCMV-seropositive healthy donor
|
hCMV-stimulated (specific) CD69+ CD4+ T-lymphocytes isolated from human peripheral blood of male hCMV-seropositive healthy donor
|
| Sample_geo_accession | GSM313675
| | Sample_status | Public on Sep 01 2008
| | Sample_submission_date | Aug 19 2008
| | Sample_last_update_date | Aug 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the silica membrane technology NucleoSpinRNA XS (Macherey-Nagel, Düren, Germany)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total RNA from the purified cell fractions was amplified and labeled using the GeneChip two cycle cDNA synthesis kit and the GeneChip IVT labeling kit (Affymetrix Inc., Santa Clara, CA), respectively.
| | Sample_hyb_protocol | Following fragmentation, 25 microg of cRNA were hybridized to the HG-U133 Plus 2.0 Array chip (Affymetrix) according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | RNA expression values for each probe set were calculated using the RMA (“Robust Multi-array Average”) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Arancha,,Rodríguez-Caballero
| | Sample_contact_email | arocab@usal.es
| | Sample_contact_institute | Cancer Research Center. University of Salamanca.
| | Sample_contact_address | Avda. Universidad de Coimbra. Campus Unamuno
| | Sample_contact_city | Salamanca
| | Sample_contact_zip/postal_code | 37007
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM313nnn/GSM313675/suppl/GSM313675.CEL.gz
| | Sample_series_id | GSE12488
| | Sample_data_row_count | 54675
| | |
|
GSM313676 | GPL570 |
|
Donor3, hCMV seropositive healthy donor
|
hCMV-stimulated (specific) CD69+ CD4+ T-lymphocytes isolated from human peripheral blood
|
Donor identifier: Martin1
Sex: male
Cell purity of 98%
hCMV-stimulated (specific) CD69+ CD4+ T-lymphocytes isolated from human peripheral blood of male hCMV-seropositive healthy donor
|
hCMV-stimulated (specific) CD69+ CD4+ T-lymphocytes isolated from human peripheral blood of male hCMV-seropositive healthy donor
|
| Sample_geo_accession | GSM313676
| | Sample_status | Public on Sep 01 2008
| | Sample_submission_date | Aug 19 2008
| | Sample_last_update_date | Aug 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the silica membrane technology NucleoSpinRNA XS (Macherey-Nagel, Düren, Germany)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total RNA from the purified cell fractions was amplified and labeled using the GeneChip two cycle cDNA synthesis kit and the GeneChip IVT labeling kit (Affymetrix Inc., Santa Clara, CA), respectively.
| | Sample_hyb_protocol | Following fragmentation, 25 microg of cRNA were hybridized to the HG-U133 Plus 2.0 Array chip (Affymetrix) according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | RNA expression values for each probe set were calculated using the RMA (“Robust Multi-array Average”) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Arancha,,Rodríguez-Caballero
| | Sample_contact_email | arocab@usal.es
| | Sample_contact_institute | Cancer Research Center. University of Salamanca.
| | Sample_contact_address | Avda. Universidad de Coimbra. Campus Unamuno
| | Sample_contact_city | Salamanca
| | Sample_contact_zip/postal_code | 37007
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM313nnn/GSM313676/suppl/GSM313676.CEL.gz
| | Sample_series_id | GSE12488
| | Sample_data_row_count | 54675
| | |
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GSM313680 | GPL570 |
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Donor4, hCMV seropositive healthy donor
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hCMV-stimulated (specific) CD69+ CD4+ T-lymphocytes isolated from human peripheral blood
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Donor identifier: Chema1
Sex: male
Cell purity of 98%
hCMV-stimulated (specific) CD69+ CD4+ T-lymphocytes isolated from human peripheral blood of male hCMV-seropositive healthy donor
|
hCMV-stimulated (specific) CD69+ CD4+ T-lymphocytes isolated from human peripheral blood of male hCMV-seropositive healthy donor
|
| Sample_geo_accession | GSM313680
| | Sample_status | Public on Sep 01 2008
| | Sample_submission_date | Aug 19 2008
| | Sample_last_update_date | Aug 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the silica membrane technology NucleoSpinRNA XS (Macherey-Nagel, Düren, Germany)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total RNA from the purified cell fractions was amplified and labeled using the GeneChip two cycle cDNA synthesis kit and the GeneChip IVT labeling kit (Affymetrix Inc., Santa Clara, CA), respectively.
| | Sample_hyb_protocol | Following fragmentation, 25 microg of cRNA were hybridized to the HG-U133 Plus 2.0 Array chip (Affymetrix) according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | RNA expression values for each probe set were calculated using the RMA (“Robust Multi-array Average”) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Arancha,,Rodríguez-Caballero
| | Sample_contact_email | arocab@usal.es
| | Sample_contact_institute | Cancer Research Center. University of Salamanca.
| | Sample_contact_address | Avda. Universidad de Coimbra. Campus Unamuno
| | Sample_contact_city | Salamanca
| | Sample_contact_zip/postal_code | 37007
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM313nnn/GSM313680/suppl/GSM313680.CEL.gz
| | Sample_series_id | GSE12488
| | Sample_data_row_count | 54675
| | |
|
GSM313682 | GPL570 |
|
Donor5, hCMV seropositive healthy donor
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hCMV-stimulated (specific) CD69+ CD4+ T-lymphocytes isolated from human peripheral blood
|
Donor identifier: Javier1
Sex: male
Cell purity of 98%
hCMV-stimulated (specific) CD69+ CD4+ T-lymphocytes isolated from human peripheral blood of male hCMV-seropositive healthy donor
|
hCMV-stimulated (specific) CD69+ CD4+ T-lymphocytes isolated from human peripheral blood of male hCMV-seropositive healthy donor
|
| Sample_geo_accession | GSM313682
| | Sample_status | Public on Sep 01 2008
| | Sample_submission_date | Aug 19 2008
| | Sample_last_update_date | Aug 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the silica membrane technology NucleoSpinRNA XS (Macherey-Nagel, Düren, Germany)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 100 ng of total RNA from the purified cell fractions was amplified and labeled using the GeneChip two cycle cDNA synthesis kit and the GeneChip IVT labeling kit (Affymetrix Inc., Santa Clara, CA), respectively.
| | Sample_hyb_protocol | Following fragmentation, 25 microg of cRNA were hybridized to the HG-U133 Plus 2.0 Array chip (Affymetrix) according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | RNA expression values for each probe set were calculated using the RMA (“Robust Multi-array Average”) algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Arancha,,Rodríguez-Caballero
| | Sample_contact_email | arocab@usal.es
| | Sample_contact_institute | Cancer Research Center. University of Salamanca.
| | Sample_contact_address | Avda. Universidad de Coimbra. Campus Unamuno
| | Sample_contact_city | Salamanca
| | Sample_contact_zip/postal_code | 37007
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM313nnn/GSM313682/suppl/GSM313682.CEL.gz
| | Sample_series_id | GSE12488
| | Sample_data_row_count | 54675
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