Search results for the GEO ID: GSE12498  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM314049 | GPL1261 |
|
ctrl-low-1
|
Control vector expressing cells at low density
|
NIH3T3
|
Control virus-infected NIH3T3 cells harvested at 30% confluency
|
| Sample_geo_accession | GSM314049
| | Sample_status | Public on Nov 21 2008
| | Sample_submission_date | Aug 20 2008
| | Sample_last_update_date | Nov 14 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells at indicated density were washed with PBS(-) and lysed with the lysis buffer in RNeasy kit (Qiagen) following manufacturer's instructions.
| | Sample_growth_protocol_ch1 | NIH3T3 cells expressing each constructs were cultured in 35mm dishes and harvested at 37C untill they reach at the indicated cell density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with RNeasy kit (Qiagen) followed by further purification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the Affymetrix standard one-cycle target labeling protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to the Affymetrix GeneChip Mouse Genome 430 2.0 Array. Chips were washed and stained with Streptavidin R-phycoerythrin (Invitrogen) in the Fluidics Station 450 (Affymetrix).
| | Sample_scan_protocol | Chips were scanned with the GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | After scanning the chips, expression values of probe sets were summarized with the RMA algorithm (Irizarry et al., 2003).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mitsunori,,Ota
| | Sample_contact_email | otam@cdb.riken.jp
| | Sample_contact_phone | +81-78-306-3367
| | Sample_contact_fax | +81-78-306-3363
| | Sample_contact_laboratory | Laboratory for Embryonic Induction
| | Sample_contact_department | CENTER FOR DEVELOPMENTAL BIOLOGY
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | Minatojima-minamimachi 2-2-3
| | Sample_contact_city | Chuo-ku, Kobe
| | Sample_contact_state | Hyogo
| | Sample_contact_zip/postal_code | 650-0047
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314049/suppl/GSM314049.CEL.gz
| | Sample_series_id | GSE12498
| | Sample_data_row_count | 45101
| | |
|
GSM314050 | GPL1261 |
|
ctrl-low-2
|
Control vector expressing cells at low density
|
NIH3T3
|
Control virus-infected NIH3T3 cells harvested at 30% confluency
|
| Sample_geo_accession | GSM314050
| | Sample_status | Public on Nov 21 2008
| | Sample_submission_date | Aug 20 2008
| | Sample_last_update_date | Nov 14 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells at indicated density were washed with PBS(-) and lysed with the lysis buffer in RNeasy kit (Qiagen) following manufacturer's instructions.
| | Sample_growth_protocol_ch1 | NIH3T3 cells expressing each constructs were cultured in 35mm dishes and harvested at 37C untill they reach at the indicated cell density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with RNeasy kit (Qiagen) followed by further purification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the Affymetrix standard one-cycle target labeling protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to the Affymetrix GeneChip Mouse Genome 430 2.0 Array. Chips were washed and stained with Streptavidin R-phycoerythrin (Invitrogen) in the Fluidics Station 450 (Affymetrix).
| | Sample_scan_protocol | Chips were scanned with the GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | After scanning the chips, expression values of probe sets were summarized with the RMA algorithm (Irizarry et al., 2003).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mitsunori,,Ota
| | Sample_contact_email | otam@cdb.riken.jp
| | Sample_contact_phone | +81-78-306-3367
| | Sample_contact_fax | +81-78-306-3363
| | Sample_contact_laboratory | Laboratory for Embryonic Induction
| | Sample_contact_department | CENTER FOR DEVELOPMENTAL BIOLOGY
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | Minatojima-minamimachi 2-2-3
| | Sample_contact_city | Chuo-ku, Kobe
| | Sample_contact_state | Hyogo
| | Sample_contact_zip/postal_code | 650-0047
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314050/suppl/GSM314050.CEL.gz
| | Sample_series_id | GSE12498
| | Sample_data_row_count | 45101
| | |
|
GSM314051 | GPL1261 |
|
tead2VP16-conf-1
|
Tead2-VP16 expressing cells at high density
|
NIH3T3
|
Proliferating Tead2-VP16-expressing NIH3T3 cells harvested at a density slightly exceeding the confluency of normal cells
|
| Sample_geo_accession | GSM314051
| | Sample_status | Public on Nov 21 2008
| | Sample_submission_date | Aug 20 2008
| | Sample_last_update_date | Nov 14 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells at indicated density were washed with PBS(-) and lysed with the lysis buffer in RNeasy kit (Qiagen) following manufacturer's instructions.
| | Sample_growth_protocol_ch1 | NIH3T3 cells expressing each constructs were cultured in 35mm dishes and harvested at 37C untill they reach at the indicated cell density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with RNeasy kit (Qiagen) followed by further purification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the Affymetrix standard one-cycle target labeling protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to the Affymetrix GeneChip Mouse Genome 430 2.0 Array. Chips were washed and stained with Streptavidin R-phycoerythrin (Invitrogen) in the Fluidics Station 450 (Affymetrix).
| | Sample_scan_protocol | Chips were scanned with the GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | After scanning the chips, expression values of probe sets were summarized with the RMA algorithm (Irizarry et al., 2003).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mitsunori,,Ota
| | Sample_contact_email | otam@cdb.riken.jp
| | Sample_contact_phone | +81-78-306-3367
| | Sample_contact_fax | +81-78-306-3363
| | Sample_contact_laboratory | Laboratory for Embryonic Induction
| | Sample_contact_department | CENTER FOR DEVELOPMENTAL BIOLOGY
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | Minatojima-minamimachi 2-2-3
| | Sample_contact_city | Chuo-ku, Kobe
| | Sample_contact_state | Hyogo
| | Sample_contact_zip/postal_code | 650-0047
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314051/suppl/GSM314051.CEL.gz
| | Sample_series_id | GSE12498
| | Sample_data_row_count | 45101
| | |
|
GSM314052 | GPL1261 |
|
tead2VP16-conf-2
|
Tead2-VP16 expressing cells at high density
|
NIH3T3
|
Proliferating Tead2-VP16-expressing NIH3T3 cells harvested at a density slightly exceeding the confluency of normal cells
|
| Sample_geo_accession | GSM314052
| | Sample_status | Public on Nov 21 2008
| | Sample_submission_date | Aug 20 2008
| | Sample_last_update_date | Nov 14 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells at indicated density were washed with PBS(-) and lysed with the lysis buffer in RNeasy kit (Qiagen) following manufacturer's instructions.
| | Sample_growth_protocol_ch1 | NIH3T3 cells expressing each constructs were cultured in 35mm dishes and harvested at 37C untill they reach at the indicated cell density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with RNeasy kit (Qiagen) followed by further purification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the Affymetrix standard one-cycle target labeling protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to the Affymetrix GeneChip Mouse Genome 430 2.0 Array. Chips were washed and stained with Streptavidin R-phycoerythrin (Invitrogen) in the Fluidics Station 450 (Affymetrix).
| | Sample_scan_protocol | Chips were scanned with the GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | After scanning the chips, expression values of probe sets were summarized with the RMA algorithm (Irizarry et al., 2003).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mitsunori,,Ota
| | Sample_contact_email | otam@cdb.riken.jp
| | Sample_contact_phone | +81-78-306-3367
| | Sample_contact_fax | +81-78-306-3363
| | Sample_contact_laboratory | Laboratory for Embryonic Induction
| | Sample_contact_department | CENTER FOR DEVELOPMENTAL BIOLOGY
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | Minatojima-minamimachi 2-2-3
| | Sample_contact_city | Chuo-ku, Kobe
| | Sample_contact_state | Hyogo
| | Sample_contact_zip/postal_code | 650-0047
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314052/suppl/GSM314052.CEL.gz
| | Sample_series_id | GSE12498
| | Sample_data_row_count | 45101
| | |
|
GSM314053 | GPL1261 |
|
yap-conf-1
|
Yap expressing cells at high density
|
NIH3T3
|
Proliferating Yap-expressing NIH3T3 cells harvested at a density slightly exceeding the confluency of normal cells
|
| Sample_geo_accession | GSM314053
| | Sample_status | Public on Nov 21 2008
| | Sample_submission_date | Aug 20 2008
| | Sample_last_update_date | Nov 14 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells at indicated density were washed with PBS(-) and lysed with the lysis buffer in RNeasy kit (Qiagen) following manufacturer's instructions.
| | Sample_growth_protocol_ch1 | NIH3T3 cells expressing each constructs were cultured in 35mm dishes and harvested at 37C untill they reach at the indicated cell density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with RNeasy kit (Qiagen) followed by further purification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the Affymetrix standard one-cycle target labeling protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to the Affymetrix GeneChip Mouse Genome 430 2.0 Array. Chips were washed and stained with Streptavidin R-phycoerythrin (Invitrogen) in the Fluidics Station 450 (Affymetrix).
| | Sample_scan_protocol | Chips were scanned with the GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | After scanning the chips, expression values of probe sets were summarized with the RMA algorithm (Irizarry et al., 2003).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mitsunori,,Ota
| | Sample_contact_email | otam@cdb.riken.jp
| | Sample_contact_phone | +81-78-306-3367
| | Sample_contact_fax | +81-78-306-3363
| | Sample_contact_laboratory | Laboratory for Embryonic Induction
| | Sample_contact_department | CENTER FOR DEVELOPMENTAL BIOLOGY
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | Minatojima-minamimachi 2-2-3
| | Sample_contact_city | Chuo-ku, Kobe
| | Sample_contact_state | Hyogo
| | Sample_contact_zip/postal_code | 650-0047
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314053/suppl/GSM314053.CEL.gz
| | Sample_series_id | GSE12498
| | Sample_data_row_count | 45101
| | |
|
GSM314054 | GPL1261 |
|
yap-conf-2
|
Yap expressing cells at high density
|
NIH3T3
|
Proliferating Yap-expressing NIH3T3 cells harvested at a density slightly exceeding the confluency of normal cells
|
| Sample_geo_accession | GSM314054
| | Sample_status | Public on Nov 21 2008
| | Sample_submission_date | Aug 20 2008
| | Sample_last_update_date | Nov 14 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells at indicated density were washed with PBS(-) and lysed with the lysis buffer in RNeasy kit (Qiagen) following manufacturer's instructions.
| | Sample_growth_protocol_ch1 | NIH3T3 cells expressing each constructs were cultured in 35mm dishes and harvested at 37C untill they reach at the indicated cell density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with RNeasy kit (Qiagen) followed by further purification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the Affymetrix standard one-cycle target labeling protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to the Affymetrix GeneChip Mouse Genome 430 2.0 Array. Chips were washed and stained with Streptavidin R-phycoerythrin (Invitrogen) in the Fluidics Station 450 (Affymetrix).
| | Sample_scan_protocol | Chips were scanned with the GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | After scanning the chips, expression values of probe sets were summarized with the RMA algorithm (Irizarry et al., 2003).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mitsunori,,Ota
| | Sample_contact_email | otam@cdb.riken.jp
| | Sample_contact_phone | +81-78-306-3367
| | Sample_contact_fax | +81-78-306-3363
| | Sample_contact_laboratory | Laboratory for Embryonic Induction
| | Sample_contact_department | CENTER FOR DEVELOPMENTAL BIOLOGY
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | Minatojima-minamimachi 2-2-3
| | Sample_contact_city | Chuo-ku, Kobe
| | Sample_contact_state | Hyogo
| | Sample_contact_zip/postal_code | 650-0047
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314054/suppl/GSM314054.CEL.gz
| | Sample_series_id | GSE12498
| | Sample_data_row_count | 45101
| | |
|
GSM314055 | GPL1261 |
|
tead2EnR-low-1
|
Tead2-EnR expressing cells at low density
|
NIH3T3
|
Tead2-EnR-expressing NIH3T3 cells harvested at 30% confluency of these cells
|
| Sample_geo_accession | GSM314055
| | Sample_status | Public on Nov 21 2008
| | Sample_submission_date | Aug 20 2008
| | Sample_last_update_date | Nov 14 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells at indicated density were washed with PBS(-) and lysed with the lysis buffer in RNeasy kit (Qiagen) following manufacturer's instructions.
| | Sample_growth_protocol_ch1 | NIH3T3 cells expressing each constructs were cultured in 35mm dishes and harvested at 37C untill they reach at the indicated cell density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with RNeasy kit (Qiagen) followed by further purification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the Affymetrix standard one-cycle target labeling protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to the Affymetrix GeneChip Mouse Genome 430 2.0 Array. Chips were washed and stained with Streptavidin R-phycoerythrin (Invitrogen) in the Fluidics Station 450 (Affymetrix).
| | Sample_scan_protocol | Chips were scanned with the GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | After scanning the chips, expression values of probe sets were summarized with the RMA algorithm (Irizarry et al., 2003).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mitsunori,,Ota
| | Sample_contact_email | otam@cdb.riken.jp
| | Sample_contact_phone | +81-78-306-3367
| | Sample_contact_fax | +81-78-306-3363
| | Sample_contact_laboratory | Laboratory for Embryonic Induction
| | Sample_contact_department | CENTER FOR DEVELOPMENTAL BIOLOGY
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | Minatojima-minamimachi 2-2-3
| | Sample_contact_city | Chuo-ku, Kobe
| | Sample_contact_state | Hyogo
| | Sample_contact_zip/postal_code | 650-0047
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314055/suppl/GSM314055.CEL.gz
| | Sample_series_id | GSE12498
| | Sample_data_row_count | 45101
| | |
|
GSM314056 | GPL1261 |
|
tead2EnR-low-2
|
Tead2-EnR expressing cells at low density
|
NIH3T3
|
Tead2-EnR-expressing NIH3T3 cells harvested at 30% confluency of these cells
|
| Sample_geo_accession | GSM314056
| | Sample_status | Public on Nov 21 2008
| | Sample_submission_date | Aug 20 2008
| | Sample_last_update_date | Nov 14 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells at indicated density were washed with PBS(-) and lysed with the lysis buffer in RNeasy kit (Qiagen) following manufacturer's instructions.
| | Sample_growth_protocol_ch1 | NIH3T3 cells expressing each constructs were cultured in 35mm dishes and harvested at 37C untill they reach at the indicated cell density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with RNeasy kit (Qiagen) followed by further purification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the Affymetrix standard one-cycle target labeling protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to the Affymetrix GeneChip Mouse Genome 430 2.0 Array. Chips were washed and stained with Streptavidin R-phycoerythrin (Invitrogen) in the Fluidics Station 450 (Affymetrix).
| | Sample_scan_protocol | Chips were scanned with the GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | After scanning the chips, expression values of probe sets were summarized with the RMA algorithm (Irizarry et al., 2003).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mitsunori,,Ota
| | Sample_contact_email | otam@cdb.riken.jp
| | Sample_contact_phone | +81-78-306-3367
| | Sample_contact_fax | +81-78-306-3363
| | Sample_contact_laboratory | Laboratory for Embryonic Induction
| | Sample_contact_department | CENTER FOR DEVELOPMENTAL BIOLOGY
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | Minatojima-minamimachi 2-2-3
| | Sample_contact_city | Chuo-ku, Kobe
| | Sample_contact_state | Hyogo
| | Sample_contact_zip/postal_code | 650-0047
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314056/suppl/GSM314056.CEL.gz
| | Sample_series_id | GSE12498
| | Sample_data_row_count | 45101
| | |
|
GSM314057 | GPL1261 |
|
ctrl-over-1
|
Control vector expressing cells over confluent
|
NIH3T3
|
Control virus-infected NIH3T3 cells harvested at complete confluency
|
| Sample_geo_accession | GSM314057
| | Sample_status | Public on Nov 21 2008
| | Sample_submission_date | Aug 20 2008
| | Sample_last_update_date | Nov 14 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells at indicated density were washed with PBS(-) and lysed with the lysis buffer in RNeasy kit (Qiagen) following manufacturer's instructions.
| | Sample_growth_protocol_ch1 | NIH3T3 cells expressing each constructs were cultured in 35mm dishes and harvested at 37C untill they reach at the indicated cell density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with RNeasy kit (Qiagen) followed by further purification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the Affymetrix standard one-cycle target labeling protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to the Affymetrix GeneChip Mouse Genome 430 2.0 Array. Chips were washed and stained with Streptavidin R-phycoerythrin (Invitrogen) in the Fluidics Station 450 (Affymetrix).
| | Sample_scan_protocol | Chips were scanned with the GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | After scanning the chips, expression values of probe sets were summarized with the RMA algorithm (Irizarry et al., 2003).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mitsunori,,Ota
| | Sample_contact_email | otam@cdb.riken.jp
| | Sample_contact_phone | +81-78-306-3367
| | Sample_contact_fax | +81-78-306-3363
| | Sample_contact_laboratory | Laboratory for Embryonic Induction
| | Sample_contact_department | CENTER FOR DEVELOPMENTAL BIOLOGY
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | Minatojima-minamimachi 2-2-3
| | Sample_contact_city | Chuo-ku, Kobe
| | Sample_contact_state | Hyogo
| | Sample_contact_zip/postal_code | 650-0047
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314057/suppl/GSM314057.CEL.gz
| | Sample_series_id | GSE12498
| | Sample_data_row_count | 45101
| | |
|
GSM314058 | GPL1261 |
|
ctrl-over-2
|
Control vector expressing cells over confluent
|
NIH3T3
|
Control virus-infected NIH3T3 cells harvested at complete confluency
|
| Sample_geo_accession | GSM314058
| | Sample_status | Public on Nov 21 2008
| | Sample_submission_date | Aug 20 2008
| | Sample_last_update_date | Nov 14 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells at indicated density were washed with PBS(-) and lysed with the lysis buffer in RNeasy kit (Qiagen) following manufacturer's instructions.
| | Sample_growth_protocol_ch1 | NIH3T3 cells expressing each constructs were cultured in 35mm dishes and harvested at 37C untill they reach at the indicated cell density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with RNeasy kit (Qiagen) followed by further purification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the Affymetrix standard one-cycle target labeling protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to the Affymetrix GeneChip Mouse Genome 430 2.0 Array. Chips were washed and stained with Streptavidin R-phycoerythrin (Invitrogen) in the Fluidics Station 450 (Affymetrix).
| | Sample_scan_protocol | Chips were scanned with the GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | After scanning the chips, expression values of probe sets were summarized with the RMA algorithm (Irizarry et al., 2003).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mitsunori,,Ota
| | Sample_contact_email | otam@cdb.riken.jp
| | Sample_contact_phone | +81-78-306-3367
| | Sample_contact_fax | +81-78-306-3363
| | Sample_contact_laboratory | Laboratory for Embryonic Induction
| | Sample_contact_department | CENTER FOR DEVELOPMENTAL BIOLOGY
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | Minatojima-minamimachi 2-2-3
| | Sample_contact_city | Chuo-ku, Kobe
| | Sample_contact_state | Hyogo
| | Sample_contact_zip/postal_code | 650-0047
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314058/suppl/GSM314058.CEL.gz
| | Sample_series_id | GSE12498
| | Sample_data_row_count | 45101
| | |
|
GSM314059 | GPL1261 |
|
tead2VP16-over-1
|
Tead2-VP16 expressing cells over confluent
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NIH3T3
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Tead2-VP16-expressing NIH3T3 cells harvested at complete confluency
|
| Sample_geo_accession | GSM314059
| | Sample_status | Public on Nov 21 2008
| | Sample_submission_date | Aug 20 2008
| | Sample_last_update_date | Nov 14 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells at indicated density were washed with PBS(-) and lysed with the lysis buffer in RNeasy kit (Qiagen) following manufacturer's instructions.
| | Sample_growth_protocol_ch1 | NIH3T3 cells expressing each constructs were cultured in 35mm dishes and harvested at 37C untill they reach at the indicated cell density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with RNeasy kit (Qiagen) followed by further purification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the Affymetrix standard one-cycle target labeling protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to the Affymetrix GeneChip Mouse Genome 430 2.0 Array. Chips were washed and stained with Streptavidin R-phycoerythrin (Invitrogen) in the Fluidics Station 450 (Affymetrix).
| | Sample_scan_protocol | Chips were scanned with the GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | After scanning the chips, expression values of probe sets were summarized with the RMA algorithm (Irizarry et al., 2003).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mitsunori,,Ota
| | Sample_contact_email | otam@cdb.riken.jp
| | Sample_contact_phone | +81-78-306-3367
| | Sample_contact_fax | +81-78-306-3363
| | Sample_contact_laboratory | Laboratory for Embryonic Induction
| | Sample_contact_department | CENTER FOR DEVELOPMENTAL BIOLOGY
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | Minatojima-minamimachi 2-2-3
| | Sample_contact_city | Chuo-ku, Kobe
| | Sample_contact_state | Hyogo
| | Sample_contact_zip/postal_code | 650-0047
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314059/suppl/GSM314059.CEL.gz
| | Sample_series_id | GSE12498
| | Sample_data_row_count | 45101
| | |
|
GSM314060 | GPL1261 |
|
tead2VP16-over-2
|
Tead2-VP16 expressing cells over confluent
|
NIH3T3
|
Tead2-VP16-expressing NIH3T3 cells harvested at complete confluency
|
| Sample_geo_accession | GSM314060
| | Sample_status | Public on Nov 21 2008
| | Sample_submission_date | Aug 20 2008
| | Sample_last_update_date | Nov 14 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells at indicated density were washed with PBS(-) and lysed with the lysis buffer in RNeasy kit (Qiagen) following manufacturer's instructions.
| | Sample_growth_protocol_ch1 | NIH3T3 cells expressing each constructs were cultured in 35mm dishes and harvested at 37C untill they reach at the indicated cell density.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with RNeasy kit (Qiagen) followed by further purification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the Affymetrix standard one-cycle target labeling protocol.
| | Sample_hyb_protocol | Following fragmentation, cRNA were hybridized to the Affymetrix GeneChip Mouse Genome 430 2.0 Array. Chips were washed and stained with Streptavidin R-phycoerythrin (Invitrogen) in the Fluidics Station 450 (Affymetrix).
| | Sample_scan_protocol | Chips were scanned with the GeneChip Scanner 3000 7G (Affymetrix).
| | Sample_data_processing | After scanning the chips, expression values of probe sets were summarized with the RMA algorithm (Irizarry et al., 2003).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Mitsunori,,Ota
| | Sample_contact_email | otam@cdb.riken.jp
| | Sample_contact_phone | +81-78-306-3367
| | Sample_contact_fax | +81-78-306-3363
| | Sample_contact_laboratory | Laboratory for Embryonic Induction
| | Sample_contact_department | CENTER FOR DEVELOPMENTAL BIOLOGY
| | Sample_contact_institute | RIKEN
| | Sample_contact_address | Minatojima-minamimachi 2-2-3
| | Sample_contact_city | Chuo-ku, Kobe
| | Sample_contact_state | Hyogo
| | Sample_contact_zip/postal_code | 650-0047
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314060/suppl/GSM314060.CEL.gz
| | Sample_series_id | GSE12498
| | Sample_data_row_count | 45101
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