Search results for the GEO ID: GSE12513 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM314405 | GPL570 |
|
FA6 transiently transfected with AGR2 siRNA, transfection C
|
pancreatic cancer cell line FA6 transiently transfected with AGR2 siRNA
|
pancreatic cancer cell line FA6 transiently transfected with AGR2 siRNA
|
gene expression data frompancreatic cancer cell line FA6 transiently transfected with AGR2 siRNA
|
Sample_geo_accession | GSM314405
| Sample_status | Public on Jul 28 2013
| Sample_submission_date | Aug 21 2008
| Sample_last_update_date | Jul 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | cells were transfected with siRNA using INTERFERin 48 hours before RNA extraction.
| Sample_growth_protocol_ch1 | FA6 cells were maintained in DMEM with 10% FBS
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | extraction of total RNA was performed using Ambion RNAqueous kit according to the manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000.
| Sample_data_processing | RMA robust multi-array analysis using R
| Sample_platform_id | GPL570
| Sample_contact_name | Hannah,,Whiteman
| Sample_contact_email | Hannah.Whiteman@cancer.org.uk
| Sample_contact_institute | QMUL
| Sample_contact_address | Charterhouse Square
| Sample_contact_city | London
| Sample_contact_zip/postal_code | EC | Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314405/suppl/GSM314405.CEL.gz
| Sample_series_id | GSE12513
| Sample_data_row_count | 54675
| |
|
GSM314406 | GPL570 |
|
FA6 transiently transfected with AGR2 siRNA, transfection A
|
pancreatic cancer cell line FA6 transiently transfected with AGR2 siRNA
|
pancreatic cancer cell line FA6 transiently transfected with AGR2 siRNA
|
gene expression data frompancreatic cancer cell line FA6 transiently transfected with AGR2 siRNA
|
Sample_geo_accession | GSM314406
| Sample_status | Public on Jul 28 2013
| Sample_submission_date | Aug 21 2008
| Sample_last_update_date | Jul 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | cells were transfected with siRNA using INTERFERin 48 hours before RNA extraction.
| Sample_growth_protocol_ch1 | FA6 cells were maintained in DMEM with 10% FBS
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | extraction of total RNA was performed using Ambion RNAqueous kit according to the manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000.
| Sample_data_processing | RMA robust multi-array analysis using R
| Sample_platform_id | GPL570
| Sample_contact_name | Hannah,,Whiteman
| Sample_contact_email | Hannah.Whiteman@cancer.org.uk
| Sample_contact_institute | QMUL
| Sample_contact_address | Charterhouse Square
| Sample_contact_city | London
| Sample_contact_zip/postal_code | EC | Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314406/suppl/GSM314406.CEL.gz
| Sample_series_id | GSE12513
| Sample_data_row_count | 54675
| |
|
GSM314407 | GPL570 |
|
FA6 transiently transfected with AGR2 siRNA, transfection B
|
pancreatic cancer cell line FA6 transiently transfected with AGR2 siRNA
|
pancreatic cancer cell line FA6 transiently transfected with AGR2 siRNA
|
gene expression data frompancreatic cancer cell line FA6 transiently transfected with AGR2 siRNA
|
Sample_geo_accession | GSM314407
| Sample_status | Public on Jul 28 2013
| Sample_submission_date | Aug 21 2008
| Sample_last_update_date | Jul 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | cells were transfected with siRNA using INTERFERin 48 hours before RNA extraction.
| Sample_growth_protocol_ch1 | FA6 cells were maintained in DMEM with 10% FBS
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | extraction of total RNA was performed using Ambion RNAqueous kit according to the manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000.
| Sample_data_processing | RMA robust multi-array analysis using R
| Sample_platform_id | GPL570
| Sample_contact_name | Hannah,,Whiteman
| Sample_contact_email | Hannah.Whiteman@cancer.org.uk
| Sample_contact_institute | QMUL
| Sample_contact_address | Charterhouse Square
| Sample_contact_city | London
| Sample_contact_zip/postal_code | EC | Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314407/suppl/GSM314407.CEL.gz
| Sample_series_id | GSE12513
| Sample_data_row_count | 54675
| |
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GSM314408 | GPL570 |
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FA6 transiently transfected with non-targeting siRNA, transfection C
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pancreatic cancer cell line FA6 transiently transfected with non-targeting siRNA
|
pancreatic cancer cell line FA6 transiently transfected with non-targeting siRNA
|
gene expression data frompancreatic cancer cell line FA6 transiently transfected with non-targeting siRNA
|
Sample_geo_accession | GSM314408
| Sample_status | Public on Jul 28 2013
| Sample_submission_date | Aug 21 2008
| Sample_last_update_date | Jul 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | cells were transfected with siRNA using INTERFERin 48 hours before RNA extraction.
| Sample_growth_protocol_ch1 | FA6 cells were maintained in DMEM with 10% FBS
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | extraction of total RNA was performed using Ambion RNAqueous kit according to the manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000.
| Sample_data_processing | RMA robust multi-array analysis using R
| Sample_platform_id | GPL570
| Sample_contact_name | Hannah,,Whiteman
| Sample_contact_email | Hannah.Whiteman@cancer.org.uk
| Sample_contact_institute | QMUL
| Sample_contact_address | Charterhouse Square
| Sample_contact_city | London
| Sample_contact_zip/postal_code | EC | Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314408/suppl/GSM314408.CEL.gz
| Sample_series_id | GSE12513
| Sample_data_row_count | 54675
| |
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GSM314409 | GPL570 |
|
FA6 transiently transfected with non-targeting siRNA, transfection A
|
pancreatic cancer cell line FA6 transiently transfected with non-targeting siRNA
|
pancreatic cancer cell line FA6 transiently transfected with non-targeting siRNA
|
gene expression data frompancreatic cancer cell line FA6 transiently transfected with non-targeting siRNA
|
Sample_geo_accession | GSM314409
| Sample_status | Public on Jul 28 2013
| Sample_submission_date | Aug 21 2008
| Sample_last_update_date | Jul 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | cells were transfected with siRNA using INTERFERin 48 hours before RNA extraction.
| Sample_growth_protocol_ch1 | FA6 cells were maintained in DMEM with 10% FBS
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | extraction of total RNA was performed using Ambion RNAqueous kit according to the manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000.
| Sample_data_processing | RMA robust multi-array analysis using R
| Sample_platform_id | GPL570
| Sample_contact_name | Hannah,,Whiteman
| Sample_contact_email | Hannah.Whiteman@cancer.org.uk
| Sample_contact_institute | QMUL
| Sample_contact_address | Charterhouse Square
| Sample_contact_city | London
| Sample_contact_zip/postal_code | EC | Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314409/suppl/GSM314409.CEL.gz
| Sample_series_id | GSE12513
| Sample_data_row_count | 54675
| |
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GSM314410 | GPL570 |
|
FA6 transiently transfected with non-targeting siRNA, transfection B
|
pancreatic cancer cell line FA6 transiently transfected with non-targeting siRNA
|
pancreatic cancer cell line FA6 transiently transfected with non-targeting siRNA
|
gene expression data frompancreatic cancer cell line FA6 transiently transfected with non-targeting siRNA
|
Sample_geo_accession | GSM314410
| Sample_status | Public on Jul 28 2013
| Sample_submission_date | Aug 21 2008
| Sample_last_update_date | Jul 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | cells were transfected with siRNA using INTERFERin 48 hours before RNA extraction.
| Sample_growth_protocol_ch1 | FA6 cells were maintained in DMEM with 10% FBS
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | extraction of total RNA was performed using Ambion RNAqueous kit according to the manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000.
| Sample_data_processing | RMA robust multi-array analysis using R
| Sample_platform_id | GPL570
| Sample_contact_name | Hannah,,Whiteman
| Sample_contact_email | Hannah.Whiteman@cancer.org.uk
| Sample_contact_institute | QMUL
| Sample_contact_address | Charterhouse Square
| Sample_contact_city | London
| Sample_contact_zip/postal_code | EC | Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314410/suppl/GSM314410.CEL.gz
| Sample_series_id | GSE12513
| Sample_data_row_count | 54675
| |
|
GSM314411 | GPL570 |
|
MiaPaCa2 pCEP4 clone 1
|
pancreatic cancer cell line MiaPaCa2 containing empty vector pCEP4
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pancreatic cancer cell line MiaPaCa2 stabley transfected with empty vector pCEP4
|
gene expression data from pancreatic cancer cell line MiaPaCa2 stabley transfected with empty vector pCEP4
|
Sample_geo_accession | GSM314411
| Sample_status | Public on Jul 28 2013
| Sample_submission_date | Aug 21 2008
| Sample_last_update_date | Jul 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | cells were grown to 70-80% confluence before RNA extraction.
| Sample_growth_protocol_ch1 | MiaPaCa2 stable cell lines were maintained in DMEM with 10% FBS
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | extraction of total RNA was performed using Ambion RNAqueous kit according to the manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000.
| Sample_data_processing | RMA robust multi-array analysis using R
| Sample_platform_id | GPL570
| Sample_contact_name | Hannah,,Whiteman
| Sample_contact_email | Hannah.Whiteman@cancer.org.uk
| Sample_contact_institute | QMUL
| Sample_contact_address | Charterhouse Square
| Sample_contact_city | London
| Sample_contact_zip/postal_code | EC | Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314411/suppl/GSM314411.CEL.gz
| Sample_series_id | GSE12513
| Sample_data_row_count | 54675
| |
|
GSM314412 | GPL570 |
|
MiaPaCa2 pCEP4 clone 3
|
pancreatic cancer cell line MiaPaCa2 containing empty vector pCEP4
|
pancreatic cancer cell line MiaPaCa2 stabley transfected with empty vector pCEP4
|
gene expression data from pancreatic cancer cell line MiaPaCa2 stabley transfected with empty vector pCEP4
|
Sample_geo_accession | GSM314412
| Sample_status | Public on Jul 28 2013
| Sample_submission_date | Aug 21 2008
| Sample_last_update_date | Jul 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | cells were grown to 70-80% confluence before RNA extraction.
| Sample_growth_protocol_ch1 | MiaPaCa2 stable cell lines were maintained in DMEM with 10% FBS
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | extraction of total RNA was performed using Ambion RNAqueous kit according to the manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000.
| Sample_data_processing | RMA robust multi-array analysis using R
| Sample_platform_id | GPL570
| Sample_contact_name | Hannah,,Whiteman
| Sample_contact_email | Hannah.Whiteman@cancer.org.uk
| Sample_contact_institute | QMUL
| Sample_contact_address | Charterhouse Square
| Sample_contact_city | London
| Sample_contact_zip/postal_code | EC | Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314412/suppl/GSM314412.CEL.gz
| Sample_series_id | GSE12513
| Sample_data_row_count | 54675
| |
|
GSM314413 | GPL570 |
|
MiaPaCa2 pCEP4 clone 5
|
pancreatic cancer cell line MiaPaCa2 containing empty vector pCEP4
|
pancreatic cancer cell line MiaPaCa2 stabley transfected with empty vector pCEP4
|
gene expression data from pancreatic cancer cell line MiaPaCa2 stabley transfected with empty vector pCEP4
|
Sample_geo_accession | GSM314413
| Sample_status | Public on Jul 28 2013
| Sample_submission_date | Aug 21 2008
| Sample_last_update_date | Jul 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | cells were grown to 70-80% confluence before RNA extraction.
| Sample_growth_protocol_ch1 | MiaPaCa2 stable cell lines were maintained in DMEM with 10% FBS
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | extraction of total RNA was performed using Ambion RNAqueous kit according to the manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000.
| Sample_data_processing | RMA robust multi-array analysis using R
| Sample_platform_id | GPL570
| Sample_contact_name | Hannah,,Whiteman
| Sample_contact_email | Hannah.Whiteman@cancer.org.uk
| Sample_contact_institute | QMUL
| Sample_contact_address | Charterhouse Square
| Sample_contact_city | London
| Sample_contact_zip/postal_code | EC | Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314413/suppl/GSM314413.CEL.gz
| Sample_series_id | GSE12513
| Sample_data_row_count | 54675
| |
|
GSM314414 | GPL570 |
|
MiaPaCa2 pCEP4 - AGR2 clone 15
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pancreatic cancer cell line MiaPaCa2 containing AGR2 overexpressing vector pCEP4 -AGR2
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pancreatic cancer cell line MiaPaCa2 stabley transfected with AGR2 overexpressing vector pCEP4 -AGR2
|
gene expression data from pancreatic cancer cell line MiaPaCa2 stabley transfected with AGR2 overexpressing vector pCEP4 -AGR2
|
Sample_geo_accession | GSM314414
| Sample_status | Public on Jul 28 2013
| Sample_submission_date | Aug 21 2008
| Sample_last_update_date | Jul 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | cells were grown to 70-80% confluence before RNA extraction.
| Sample_growth_protocol_ch1 | MiaPaCa2 stable cell lines were maintained in DMEM with 10% FBS
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | extraction of total RNA was performed using Ambion RNAqueous kit according to the manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000.
| Sample_data_processing | RMA robust multi-array analysis using R
| Sample_platform_id | GPL570
| Sample_contact_name | Hannah,,Whiteman
| Sample_contact_email | Hannah.Whiteman@cancer.org.uk
| Sample_contact_institute | QMUL
| Sample_contact_address | Charterhouse Square
| Sample_contact_city | London
| Sample_contact_zip/postal_code | EC | Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314414/suppl/GSM314414.CEL.gz
| Sample_series_id | GSE12513
| Sample_data_row_count | 54675
| |
|
GSM314415 | GPL570 |
|
MiaPaCa2 pCEP4 - AGR2 clone 16
|
pancreatic cancer cell line MiaPaCa2 containing AGR2 overexpressing vector pCEP4 -AGR2
|
pancreatic cancer cell line MiaPaCa2 stabley transfected with AGR2 overexpressing vector pCEP4 -AGR2
|
gene expression data from pancreatic cancer cell line MiaPaCa2 stabley transfected with AGR2 overexpressing vector pCEP4 -AGR2
|
Sample_geo_accession | GSM314415
| Sample_status | Public on Jul 28 2013
| Sample_submission_date | Aug 21 2008
| Sample_last_update_date | Jul 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | cells were grown to 70-80% confluence before RNA extraction.
| Sample_growth_protocol_ch1 | MiaPaCa2 stable cell lines were maintained in DMEM with 10% FBS
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | extraction of total RNA was performed using Ambion RNAqueous kit according to the manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000.
| Sample_data_processing | RMA robust multi-array analysis using R
| Sample_platform_id | GPL570
| Sample_contact_name | Hannah,,Whiteman
| Sample_contact_email | Hannah.Whiteman@cancer.org.uk
| Sample_contact_institute | QMUL
| Sample_contact_address | Charterhouse Square
| Sample_contact_city | London
| Sample_contact_zip/postal_code | EC | Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314415/suppl/GSM314415.CEL.gz
| Sample_series_id | GSE12513
| Sample_data_row_count | 54675
| |
|
GSM314416 | GPL570 |
|
MiaPaCa2 pCEP4 - AGR2 clone 2
|
pancreatic cancer cell line MiaPaCa2 containing AGR2 overexpressing vector pCEP4 -AGR2
|
pancreatic cancer cell line MiaPaCa2 stabley transfected with AGR2 overexpressing vector pCEP4 -AGR2
|
gene expression data from pancreatic cancer cell line MiaPaCa2 stabley transfected with AGR2 overexpressing vector pCEP4 -AGR2
|
Sample_geo_accession | GSM314416
| Sample_status | Public on Jul 28 2013
| Sample_submission_date | Aug 21 2008
| Sample_last_update_date | Jul 28 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | cells were grown to 70-80% confluence before RNA extraction.
| Sample_growth_protocol_ch1 | MiaPaCa2 stable cell lines were maintained in DMEM with 10% FBS
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | extraction of total RNA was performed using Ambion RNAqueous kit according to the manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000.
| Sample_data_processing | RMA robust multi-array analysis using R
| Sample_platform_id | GPL570
| Sample_contact_name | Hannah,,Whiteman
| Sample_contact_email | Hannah.Whiteman@cancer.org.uk
| Sample_contact_institute | QMUL
| Sample_contact_address | Charterhouse Square
| Sample_contact_city | London
| Sample_contact_zip/postal_code | EC | Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314416/suppl/GSM314416.CEL.gz
| Sample_series_id | GSE12513
| Sample_data_row_count | 54675
| |
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