Result

Search results for the GEO ID: GSE12524

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM314669

GPL570

mock3

293 cells without tRNase ZL

human embryonic kidney 293 cell

293 without tRNase ZL

Sample_geo_accession	GSM314669
Sample_status	Public on Jun 15 2009
Sample_submission_date	Aug 22 2008
Sample_last_update_date	Jun 15 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The 293 cells were transfected with the tRNase ZL plasmid using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol.
Sample_growth_protocol_ch1	The 293 cells were cultured in Dulbecco’s modified Eagle’s medium (Sigma) supplemented with 10% fetal bovine serum (MP Biomedicals) and 1% penicillin-streptomycin (Invitrogen) at 37 °C in 5% CO2 humidified incubator.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with ISOGEN according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cDNA were synthesized by GeneChip T7-Oligo(dT) Promoter Primer Kit（Affymetrix) and TaKaRa cDNA Synthesis Kit （TaKaRa Bio） from 3ug total RNA. Biotinylated cRNA were synthesized by IVT Labeling Kit(Affymetrix)
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 7G.
Sample_data_processing	Single Array Analysis were calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Masayuki,,Nashimoto
Sample_contact_email	mnashimoto@nupals.ac.jp
Sample_contact_phone	+81 250 25 5119
Sample_contact_fax	+81 250 25 5021
Sample_contact_institute	Niigata University of Pharmacy and Applied Life Sciences
Sample_contact_address	Higashijima 265-1
Sample_contact_city	Niigata
Sample_contact_zip/postal_code	956 8603
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314669/suppl/GSM314669.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314669/suppl/GSM314669.CHP.gz
Sample_series_id	GSE12524
Sample_data_row_count	54675

GSM314670

GPL570

OE/ZL3

293 cells with tRNase ZL

human embryonic kidney 293 cell

293 with tRNase ZL

Sample_geo_accession	GSM314670
Sample_status	Public on Jun 15 2009
Sample_submission_date	Aug 22 2008
Sample_last_update_date	Jun 15 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The 293 cells were transfected with the tRNase ZL plasmid using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol.
Sample_growth_protocol_ch1	The 293 cells were cultured in Dulbecco’s modified Eagle’s medium (Sigma) supplemented with 10% fetal bovine serum (MP Biomedicals) and 1% penicillin-streptomycin (Invitrogen) at 37 °C in 5% CO2 humidified incubator.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with ISOGEN according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	cDNA were synthesized by GeneChip T7-Oligo(dT) Promoter Primer Kit（Affymetrix) and TaKaRa cDNA Synthesis Kit （TaKaRa Bio） from 3ug total RNA. Biotinylated cRNA were synthesized by IVT Labeling Kit(Affymetrix)
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using GeneChip Scanner 3000 7G.
Sample_data_processing	Single Array Analysis were calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Masayuki,,Nashimoto
Sample_contact_email	mnashimoto@nupals.ac.jp
Sample_contact_phone	+81 250 25 5119
Sample_contact_fax	+81 250 25 5021
Sample_contact_institute	Niigata University of Pharmacy and Applied Life Sciences
Sample_contact_address	Higashijima 265-1
Sample_contact_city	Niigata
Sample_contact_zip/postal_code	956 8603
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314670/suppl/GSM314670.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM314nnn/GSM314670/suppl/GSM314670.CHP.gz
Sample_series_id	GSE12524
Sample_data_row_count	54675

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