Search results for the GEO ID: GSE12583  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM315617 | GPL570 |
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Human foreskin fibroblast 1
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Fibroblast 1
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Human foreskin fibroblast
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Gene expression of human foreskin fibroblasts
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| Sample_geo_accession | GSM315617
| | Sample_status | Public on Oct 27 2008
| | Sample_submission_date | Aug 27 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | dermis of foreskin biopsy
| | Sample_treatment_protocol_ch1 | Fibroblasts were isolated by explant outgrowth from dermis of foreskin biopsy using DMEM, 10% bovine serum, Pen/Strep, 1% Glutamax. Passage 4 growing on uncoated dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from fibroblasts from individual 1 using Trizol (Invitrogen). The amplification was done following the Nugen protocol (Ovation RNA Amplification System V2) with 25ng starting material.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling of 3.75 ug ssDNA following the Nugen protocol FL-Ovation cDNA Biotin Module V2.
| | Sample_hyb_protocol | Standard affymetrix protocol
| | Sample_scan_protocol | Default settings for the Affymetrix GeneChip Scanner (7G upgrade)
| | Sample_data_processing | Data extraction is done by the Affymetrix GCOS software v1.4. Raw data were imported in R and normalized using the GCRMA algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephanie,,Boue
| | Sample_contact_institute | CMRB
| | Sample_contact_address | Dr Aiguader 88
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08003
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM315nnn/GSM315617/suppl/GSM315617.CEL.gz
| | Sample_series_id | GSE12583
| | Sample_data_row_count | 54675
| | |
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GSM315618 | GPL570 |
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Human foreskin fibroblast 2
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Human foreskin fibroblast 2
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Human foreskin fibroblast
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Gene expression of human foreskin fibroblasts
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| Sample_geo_accession | GSM315618
| | Sample_status | Public on Oct 27 2008
| | Sample_submission_date | Aug 27 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | dermis of foreskin biopsy
| | Sample_treatment_protocol_ch1 | Fibroblasts were isolated by explant outgrowth from dermis of foreskin biopsy using DMEM, 10% bovine serum, Pen/Strep, 1% Glutamax. Passage 4 growing on uncoated dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from fibroblasts from individual 1 using Trizol (Invitrogen). The amplification was done following the Nugen protocol (Ovation RNA Amplification System V2) with 25ng starting material.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling of 3.75 ug ssDNA following the Nugen protocol FL-Ovation cDNA Biotin Module V2.
| | Sample_hyb_protocol | Standard affymetrix protocol
| | Sample_scan_protocol | settings for the Affymetrix GeneChip Scanner (7G upgrade)
| | Sample_data_processing | Data extraction was done by the Affymetrix GCOS software v1.4. Raw data were imported in R and normalized using the GCRMA algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephanie,,Boue
| | Sample_contact_institute | CMRB
| | Sample_contact_address | Dr Aiguader 88
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08003
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM315nnn/GSM315618/suppl/GSM315618.CEL.gz
| | Sample_series_id | GSE12583
| | Sample_data_row_count | 54675
| | |
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GSM315619 | GPL570 |
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Human foreskin keratinocyte 1
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Human foreskin keratinocyte - 16 years old patient
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Human foreskin keratinocyte from a 16-year-old male
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Gene expression of human foreskin keratinocytes
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| Sample_geo_accession | GSM315619
| | Sample_status | Public on Oct 27 2008
| | Sample_submission_date | Aug 27 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | dermis of foreskin biopsy
| | Sample_treatment_protocol_ch1 | Keratinocytes were isolated from juvenile foreskins using dispase to remove the dermis from the epidermis followed by trypsinization.
| | Sample_growth_protocol_ch1 | culture in serum free low calcium medium (Epilife, Invitrogen). Used at passage 4 growing on uncoated dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from fibroblasts from individual 1 using Trizol (Invitrogen). The amplification was done following the Nugen protocol (Ovation RNA Amplification System V2) with 25ng starting material.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling of 3.75 ug ssDNA following the Nugen protocol FL-Ovation cDNA Biotin Module V2.
| | Sample_hyb_protocol | Standard affymetrix protocol
| | Sample_scan_protocol | settings for the Affymetrix GeneChip Scanner (7G upgrade)
| | Sample_data_processing | Data extraction was done by the Affymetrix GCOS software v1.4. Raw data were imported in R and normalized using the GCRMA algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephanie,,Boue
| | Sample_contact_institute | CMRB
| | Sample_contact_address | Dr Aiguader 88
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08003
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM315nnn/GSM315619/suppl/GSM315619.CEL.gz
| | Sample_series_id | GSE12583
| | Sample_data_row_count | 54675
| | |
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GSM315620 | GPL570 |
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Human foreskin keratinocyte 2
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Human foreskin keratinocyte - 4 years old male
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Human foreskin keratinocyte from a 4-year-old male
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Gene expression of human foreskin keratinocytes
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| Sample_geo_accession | GSM315620
| | Sample_status | Public on Oct 27 2008
| | Sample_submission_date | Aug 27 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | dermis of foreskin biopsy
| | Sample_treatment_protocol_ch1 | Keratinocytes were isolated from juvenile foreskins using dispase to remove the dermis from the epidermis followed by trypsinization.
| | Sample_growth_protocol_ch1 | culture in serum free low calcium medium (Epilife, Invitrogen). Used at passage 4 growing on uncoated dishes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from fibroblasts from individual 1 using Trizol (Invitrogen). The amplification was done following the Nugen protocol (Ovation RNA Amplification System V2) with 25ng starting material.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling of 3.75 ug ssDNA following the Nugen protocol FL-Ovation cDNA Biotin Module V2.
| | Sample_hyb_protocol | Standard affymetrix protocol
| | Sample_scan_protocol | settings for the Affymetrix GeneChip Scanner (7G upgrade)
| | Sample_data_processing | Data extraction was done by the Affymetrix GCOS software v1.4. Raw data were imported in R and normalized using the GCRMA algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephanie,,Boue
| | Sample_contact_institute | CMRB
| | Sample_contact_address | Dr Aiguader 88
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08003
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM315nnn/GSM315620/suppl/GSM315620.CEL.gz
| | Sample_series_id | GSE12583
| | Sample_data_row_count | 54675
| | |
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GSM315621 | GPL570 |
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Human ES2 cell line
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Human ES2 cell line
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human ES2 cells
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Human ES2 cell line
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| Sample_geo_accession | GSM315621
| | Sample_status | Public on Oct 27 2008
| | Sample_submission_date | Aug 27 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | ES2 cells were cultured on top of irradiated
| | Sample_growth_protocol_ch1 | human fibroblasts and picked mechanically. Knockout DMEM
| | Sample_growth_protocol_ch1 | ES media was supplemented with 20% knockout serum replacement,
| | Sample_growth_protocol_ch1 | non-essential amino acids, 2-Mercaptoethanol, Penicillin/Streptomycin,
| | Sample_growth_protocol_ch1 | GlutaMAX, bFGF (all Gibco) and Human albumin (Grifols). Feeder cells
| | Sample_growth_protocol_ch1 | used: HFF-1: Human Foreskin Fibroblasts, ATCC Number SCRC-1041.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from ES2 cells using Trizol (Invitrogen). The amplification was done following the Nugen protocol (Ovation RNA Amplification System V2) with 25ng starting material.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling of 3.75 ug ssDNA following the Nugen protocol FL-Ovation cDNA Biotin Module V2.
| | Sample_hyb_protocol | Standard affymetrix protocol
| | Sample_scan_protocol | Default settings for the Affymetrix GeneChip Scanner (7G upgrade)
| | Sample_data_processing | Data extraction is done by the Affymetrix GCOS software v1.4. Raw data were imported in R and normalized using the GCRMA algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephanie,,Boue
| | Sample_contact_institute | CMRB
| | Sample_contact_address | Dr Aiguader 88
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08003
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM315nnn/GSM315621/suppl/GSM315621.CEL.gz
| | Sample_series_id | GSE12583
| | Sample_data_row_count | 54675
| | |
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GSM315622 | GPL570 |
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Human ES4 cell line
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Human ES4 cell line
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Human ES4 cell line
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Human ES4 cell line
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| Sample_geo_accession | GSM315622
| | Sample_status | Public on Oct 27 2008
| | Sample_submission_date | Aug 27 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | ES4 cells were cultured on matrigel by
| | Sample_growth_protocol_ch1 | trypsinization (using mouse fibroblast conditioned media). Knockout DMEM
| | Sample_growth_protocol_ch1 | ES media was supplemented with 20% knockout serum replacement,
| | Sample_growth_protocol_ch1 | non-essential amino acids, 2-Mercaptoethanol, Penicillin/Streptomycin,
| | Sample_growth_protocol_ch1 | GlutaMAX, bFGF (all Gibco) and Human albumin (Grifols). Feeder cells
| | Sample_growth_protocol_ch1 | used: HFF-1: Human Foreskin Fibroblasts, ATCC Number SCRC-1041.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from ES4 cells using trizol (Invitrogen). The amplification was done following the Nugen protocol (Ovation RNA Amplification System V2) with 25ng starting material.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling of 3.75 ug ssDNA following the Nugen protocol FL-Ovation cDNA Biotin Module V2.
| | Sample_hyb_protocol | Standard affymetrix protocol
| | Sample_scan_protocol | Default settings for the Affymetrix GeneChip Scanner (7G upgrade)
| | Sample_data_processing | Data extraction is done by the Affymetrix GCOS software v1.4. Raw data were imported in R and normalized using the GCRMA algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephanie,,Boue
| | Sample_contact_institute | CMRB
| | Sample_contact_address | Dr Aiguader 88
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08003
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM315nnn/GSM315622/suppl/GSM315622.CEL.gz
| | Sample_series_id | GSE12583
| | Sample_data_row_count | 54675
| | |
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GSM315623 | GPL570 |
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KiPS obtained from reprogramming human keratinocytes with 3 factors (Oct4, Sox2, Klf4)
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KiPS3F1
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KiPS obtained from reprogramming keratinocytes with 3 factors (Oct4, Sox2, Klf4)
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Human pluripotent KiPS3F1 cell line
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| Sample_geo_accession | GSM315623
| | Sample_status | Public on Oct 27 2008
| | Sample_submission_date | Aug 27 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | KiPS were obtained after infection of human foreskin keratinocytes with 3 retroviruses (Oct4, Sox2, Klf4).
| | Sample_growth_protocol_ch1 | KiPS3F1 cells were cultured on matrigel by
| | Sample_growth_protocol_ch1 | trypsinization (using mouse fibroblast conditioned media). Knockout DMEM
| | Sample_growth_protocol_ch1 | ES media was supplemented with 20% knockout serum replacement,
| | Sample_growth_protocol_ch1 | non-essential amino acids, 2-Mercaptoethanol, Penicillin/Streptomycin,
| | Sample_growth_protocol_ch1 | GlutaMAX, bFGF (all Gibco) and Human albumin (Grifols). Feeder cells
| | Sample_growth_protocol_ch1 | used: HFF-1: Human Foreskin Fibroblasts, ATCC Number SCRC-1041.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from KiPS3F1 cells using trizol (Invitrogen). The amplification was done following the Nugen protocol (Ovation RNA Amplification System V2) with 25ng starting material.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling of 3.75 ug ssDNA following the Nugen protocol FL-Ovation cDNA Biotin Module V2.
| | Sample_hyb_protocol | Standard affymetrix protocol
| | Sample_scan_protocol | Default settings for the Affymetrix GeneChip Scanner (7G upgrade)
| | Sample_data_processing | Data extraction is done by the Affymetrix GCOS software v1.4. Raw data were imported in R and normalized using the GCRMA algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephanie,,Boue
| | Sample_contact_institute | CMRB
| | Sample_contact_address | Dr Aiguader 88
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08003
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM315nnn/GSM315623/suppl/GSM315623.CEL.gz
| | Sample_series_id | GSE12583
| | Sample_data_row_count | 54675
| | |
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GSM315624 | GPL570 |
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KiPS obtained from reprogramming human keratinocytes with 4 factors (Oct4, Sox2, Klf4, cmyc) 1
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KiPS4F1
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Pluripotent KiPS cell lines obtained from reprogramming human foreskin keratinocytes with 4 factors (Oct4, Sox2, Klf4, cmyc)
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Human pluripotent KiPS4F1 cell line
|
| Sample_geo_accession | GSM315624
| | Sample_status | Public on Oct 27 2008
| | Sample_submission_date | Aug 27 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | KiPS were obtained after infection of human foreskin keratinocytes with 4 retroviruses (Oct4, Sox2, Klf4, cmyc).
| | Sample_growth_protocol_ch1 | KiPS4F1 cells were cultured on matrigel by
| | Sample_growth_protocol_ch1 | trypsinization (using mouse fibroblast conditioned media). Knockout DMEM
| | Sample_growth_protocol_ch1 | ES media was supplemented with 20% knockout serum replacement,
| | Sample_growth_protocol_ch1 | non-essential amino acids, 2-Mercaptoethanol, Penicillin/Streptomycin,
| | Sample_growth_protocol_ch1 | GlutaMAX, bFGF (all Gibco) and Human albumin (Grifols). Feeder cells
| | Sample_growth_protocol_ch1 | used: HFF-1: Human Foreskin Fibroblasts, ATCC Number SCRC-1041.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from KiPS4F1 cells using trizol (Invitrogen). The amplification was done following the Nugen protocol (Ovation RNA Amplification System V2) with 25ng starting material.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling of 3.75 ug ssDNA following the Nugen protocol FL-Ovation cDNA Biotin Module V2.
| | Sample_hyb_protocol | Standard affymetrix protocol
| | Sample_scan_protocol | Default settings for the Affymetrix GeneChip Scanner (7G upgrade)
| | Sample_data_processing | Data extraction is done by the Affymetrix GCOS software v1.4. Raw data were imported in R and normalized using the GCRMA algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephanie,,Boue
| | Sample_contact_institute | CMRB
| | Sample_contact_address | Dr Aiguader 88
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08003
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM315nnn/GSM315624/suppl/GSM315624.CEL.gz
| | Sample_series_id | GSE12583
| | Sample_data_row_count | 54675
| | |
|
GSM315625 | GPL570 |
|
KiPS obtained from reprogramming human keratinocytes with 4 factors (Oct4, Sox2, Klf4, cmyc) 2
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KiPS4F2
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Pluripotent KiPS cell line obtained from reprogramming human foreskin keratinocytes with 4 factors (Oct4, Sox2, Klf4, cmyc), cultured on feeders.
|
Human pluripotent KiPS4F2 cell line
|
| Sample_geo_accession | GSM315625
| | Sample_status | Public on Oct 27 2008
| | Sample_submission_date | Aug 27 2008
| | Sample_last_update_date | Oct 27 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | KiPS were obtained after infection of human foreskin keratinocytes with 4 retroviruses (Oct4, Sox2, Klf4, cmyc).
| | Sample_growth_protocol_ch1 | KiPS4F1 cells were cultured on top of irradiated
| | Sample_growth_protocol_ch1 | human fibroblasts and picked mechanically. Knockout DMEM
| | Sample_growth_protocol_ch1 | ES media was supplemented with 20% knockout serum replacement,
| | Sample_growth_protocol_ch1 | non-essential amino acids, 2-Mercaptoethanol, Penicillin/Streptomycin,
| | Sample_growth_protocol_ch1 | GlutaMAX, bFGF (all Gibco) and Human albumin (Grifols). Feeder cells
| | Sample_growth_protocol_ch1 | used: HFF-1: Human Foreskin Fibroblasts, ATCC Number SCRC-1041.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from KiPS4F2 cells using trizol (Invitrogen). The amplification was done following the Nugen protocol (Ovation RNA Amplification System V2) with 25ng starting material.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling of 3.75 ug ssDNA following the Nugen protocol FL-Ovation cDNA Biotin Module V2.
| | Sample_hyb_protocol | Standard affymetrix protocol
| | Sample_scan_protocol | Default settings for the Affymetrix GeneChip Scanner (7G upgrade)
| | Sample_data_processing | Data extraction is done by the Affymetrix GCOS software v1.4. Raw data were imported in R and normalized using the GCRMA algorithm.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Stephanie,,Boue
| | Sample_contact_institute | CMRB
| | Sample_contact_address | Dr Aiguader 88
| | Sample_contact_city | Barcelona
| | Sample_contact_zip/postal_code | 08003
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM315nnn/GSM315625/suppl/GSM315625.CEL.gz
| | Sample_series_id | GSE12583
| | Sample_data_row_count | 54675
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