Search results for the GEO ID: GSE12596  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM312128 | GPL1355 |
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RGC-1-OD
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Retinal Ganglion Cells of Normal and Experimental Glaucoma Model of Brown Norway rats
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Retinal Ganglion Cells isolated from adult male Brown Norway rats (300 to 450 g; Charles River, Boston, MA).
|
The NIH Neuroscience Microarray Consortium processed the GeneChip hybridization and scanning.
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| Sample_geo_accession | GSM312128
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Aug 12 2008
| | Sample_last_update_date | Oct 01 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 = Retinal ganglion cells were bilaterally back-labeled by stereotaxic injection of Fluorogold into the superior colliculus. Sustained unilateral IOP elevation was produced by hypertonic saline episcleral vein injection in Brown Norway rats with the fellow eye serving as a control (n | 3). After IOP had been elevated for 10 days, the rats were killed by asphyxiation with carbon dioxide, the eyes were rapidly removed. The eyecups were collected and frozen in optimal cutting temperature compound and sectioned. Using laser capture microdissection, 2,000 retinal ganglion cells were captured from each eyes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Isolated RGCs were processed for RNA extraction using the PicoPure RNA isolation kit (Arcturus, Mountain View, CA) according to the manufacturer’s protocol. Isolated RNA was amplified using the linear amplification as provided in the RiboAmp HS RNA amplification kit (Arcturus, Mountain View, CA)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | 10 µg of total RNA was used for cRNA labeling according to the recommended Affymetrix protocol.
| | Sample_hyb_protocol | The arrays were hybridized for 16h at 45°C in a rotisserie oven. After hybridization, arrays were washed using the Affymetrix fluidics station and stained with streptavidin-phycoerythrin according to the Affymetrix technical manual.
| | Sample_scan_protocol | Washed arrays were scanned on a Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Scanned output files were visually inspected for hybridization artifacts and then analyzed by using Affymetrix GeneChip Operation Software (GCOS). Data obtained were analyzed with DChip.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Danyi,,Wang
| | Sample_contact_email | danyi_wang@meei.harvard.edu
| | Sample_contact_laboratory | Grosskreutz's lab
| | Sample_contact_department | Ophthalmology
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | 243 Charles St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02461
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312128/suppl/GSM312128.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM312nnn/GSM312128/suppl/GSM312128.dcp.gz
| | Sample_series_id | GSE12596
| | Sample_data_row_count | 31099
| | |
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GSM335227 | GPL1355 |
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RGC-1-OS
|
Retinal Ganglion Cells of Normal and Experimental Glaucoma Model of Brown Norway rats
|
Retinal Ganglion Cells isolated from adult male Brown Norway rats (300 to 450 g; Charles River, Boston, MA)
|
The NIH Neuroscience Microarray Consortium processed the GeneChip hybridization and scanning.
|
| Sample_geo_accession | GSM335227
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Oct 20 2008
| | Sample_last_update_date | Oct 01 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 = Retinal ganglion cells were bilaterally back-labeled by stereotaxic injection of Fluorogold into the superior colliculus. Sustained unilateral IOP elevation was produced by hypertonic saline episcleral vein injection in Brown Norway rats with the fellow eye serving as a control (n | 3). After IOP had been elevated for 10 days, the rats were killed by asphyxiation with carbon dioxide, the eyes were rapidly removed. The eyecups were collected and frozen in optimal cutting temperature compound and sectioned. Using laser capture microdissection, 2,000 retinal ganglion cells were captured from each eyes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Isolated RGCs was processed for RNA extraction using the PicoPure RNA isolation kit (Arcturus, Mountain View, CA) according to the manufacturer’s protocol. Isolated RNA was amplified using the linear amplification as provided in the RiboAmp HS RNA amplification kit (Arcturus, Mountain View, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 10 µg of total RNA were used for cRNA labeling according to the recommended Affymetrix protocol.
| | Sample_hyb_protocol | The arrays were hybridized for 16h at 45°C in a rotisserie oven. After hybridization, arrays were washed using the Affymetrix fluidics station and stained with streptavidin-phycoerythrin according to the Affymetrix technical manual.
| | Sample_scan_protocol | Washed arrays were scanned on a Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Scanned output files were visually inspected for hybridization artifacts and then analyzed by using Affymetrix GeneChip Operation Software (GCOS). Data obtained were analyzed with DChip.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Danyi,,Wang
| | Sample_contact_email | danyi_wang@meei.harvard.edu
| | Sample_contact_laboratory | Grosskreutz's lab
| | Sample_contact_department | Ophthalmology
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | 243 Charles St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02461
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335227/suppl/GSM335227.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335227/suppl/GSM335227.dcp.gz
| | Sample_series_id | GSE12596
| | Sample_data_row_count | 31099
| | |
|
GSM335228 | GPL1355 |
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RGC-2-OD
|
Retinal Ganglion Cells of Normal and Experimental Glaucoma Model of Brown Norway rats
|
Retinal Ganglion Cells isolated from adult male Brown Norway rats (300 to 450 g; Charles River, Boston, MA)
|
The NIH Neuroscience Microarray Consortium processed the GeneChip hybridization and scanning.
|
| Sample_geo_accession | GSM335228
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Oct 20 2008
| | Sample_last_update_date | Oct 01 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 = Retinal ganglion cells were bilaterally back-labeled by stereotaxic injection of Fluorogold into the superior colliculus. Sustained unilateral IOP elevation was produced by hypertonic saline episcleral vein injection in Brown Norway rats with the fellow eye serving as a control (n | 3). After IOP had been elevated for 10 days, the rats were killed by asphyxiation with carbon dioxide, the eyes were rapidly removed. The eyecups were collected and frozen in optimal cutting temperature compound and sectioned. Using laser capture microdissection, 2,000 retinal ganglion cells were captured from each eyes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Isolated RGCs was processed for RNA extraction using the PicoPure RNA isolation kit (Arcturus, Mountain View, CA) according to the manufacturer’s protocol. Isolated RNA was amplified using the linear amplification as provided in the RiboAmp HS RNA amplification kit (Arcturus, Mountain View, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 10 µg of total RNA were used for cRNA labeling according to the recommended Affymetrix protocol.
| | Sample_hyb_protocol | The arrays were hybridized for 16h at 45°C in a rotisserie oven. After hybridization, arrays were washed using the Affymetrix fluidics station and stained with streptavidin-phycoerythrin according to the Affymetrix technical manual.
| | Sample_scan_protocol | Washed arrays were scanned on a Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Scanned output files were visually inspected for hybridization artifacts and then analyzed by using Affymetrix GeneChip Operation Software (GCOS). Data obtained were analyzed with DChip.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Danyi,,Wang
| | Sample_contact_email | danyi_wang@meei.harvard.edu
| | Sample_contact_laboratory | Grosskreutz's lab
| | Sample_contact_department | Ophthalmology
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | 243 Charles St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02461
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335228/suppl/GSM335228.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335228/suppl/GSM335228.dcp.gz
| | Sample_series_id | GSE12596
| | Sample_data_row_count | 31099
| | |
|
GSM335229 | GPL1355 |
|
RGC-2-OS
|
Retinal Ganglion Cells of Normal and Experimental Glaucoma Model of Brown Norway rats
|
Retinal Ganglion Cells isolated from adult male Brown Norway rats (300 to 450 g; Charles River, Boston, MA)
|
The NIH Neuroscience Microarray Consortium processed the GeneChip hybridization and scanning.
|
| Sample_geo_accession | GSM335229
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Oct 20 2008
| | Sample_last_update_date | Oct 01 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 = Retinal ganglion cells were bilaterally back-labeled by stereotaxic injection of Fluorogold into the superior colliculus. Sustained unilateral IOP elevation was produced by hypertonic saline episcleral vein injection in Brown Norway rats with the fellow eye serving as a control (n | 3). After IOP had been elevated for 10 days, the rats were killed by asphyxiation with carbon dioxide, the eyes were rapidly removed. The eyecups were collected and frozen in optimal cutting temperature compound and sectioned. Using laser capture microdissection, 2,000 retinal ganglion cells were captured from each eyes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Isolated RGCs was processed for RNA extraction using the PicoPure RNA isolation kit (Arcturus, Mountain View, CA) according to the manufacturer’s protocol. Isolated RNA was amplified using the linear amplification as provided in the RiboAmp HS RNA amplification kit (Arcturus, Mountain View, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 10 µg of total RNA were used for cRNA labeling according to the recommended Affymetrix protocol.
| | Sample_hyb_protocol | The arrays were hybridized for 16h at 45°C in a rotisserie oven. After hybridization, arrays were washed using the Affymetrix fluidics station and stained with streptavidin-phycoerythrin according to the Affymetrix technical manual.
| | Sample_scan_protocol | Washed arrays were scanned on a Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Scanned output files were visually inspected for hybridization artifacts and then analyzed by using Affymetrix GeneChip Operation Software (GCOS). Data obtained were analyzed with DChip.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Danyi,,Wang
| | Sample_contact_email | danyi_wang@meei.harvard.edu
| | Sample_contact_laboratory | Grosskreutz's lab
| | Sample_contact_department | Ophthalmology
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | 243 Charles St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02461
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335229/suppl/GSM335229.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335229/suppl/GSM335229.dcp.gz
| | Sample_series_id | GSE12596
| | Sample_data_row_count | 31099
| | |
|
GSM335230 | GPL1355 |
|
RGC-3-OD
|
Retinal Ganglion Cells of Normal and Experimental Glaucoma Model of Brown Norway rats
|
Retinal Ganglion Cells isolated from adult male Brown Norway rats (300 to 450 g; Charles River, Boston, MA)
|
The NIH Neuroscience Microarray Consortium processed the GeneChip hybridization and scanning.
|
| Sample_geo_accession | GSM335230
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Oct 20 2008
| | Sample_last_update_date | Oct 01 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 = Retinal ganglion cells were bilaterally back-labeled by stereotaxic injection of Fluorogold into the superior colliculus. Sustained unilateral IOP elevation was produced by hypertonic saline episcleral vein injection in Brown Norway rats with the fellow eye serving as a control (n | 3). After IOP had been elevated for 10 days, the rats were killed by asphyxiation with carbon dioxide, the eyes were rapidly removed. The eyecups were collected and frozen in optimal cutting temperature compound and sectioned. Using laser capture microdissection, 2,000 retinal ganglion cells were captured from each eyes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Isolated RGCs was processed for RNA extraction using the PicoPure RNA isolation kit (Arcturus, Mountain View, CA) according to the manufacturer’s protocol. Isolated RNA was amplified using the linear amplification as provided in the RiboAmp HS RNA amplification kit (Arcturus, Mountain View, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 10 µg of total RNA were used for cRNA labeling according to the recommended Affymetrix protocol.
| | Sample_hyb_protocol | The arrays were hybridized for 16h at 45°C in a rotisserie oven. After hybridization, arrays were washed using the Affymetrix fluidics station and stained with streptavidin-phycoerythrin according to the Affymetrix technical manual.
| | Sample_scan_protocol | Washed arrays were scanned on a Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Scanned output files were visually inspected for hybridization artifacts and then analyzed by using Affymetrix GeneChip Operation Software (GCOS). Data obtained were analyzed with DChip.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Danyi,,Wang
| | Sample_contact_email | danyi_wang@meei.harvard.edu
| | Sample_contact_laboratory | Grosskreutz's lab
| | Sample_contact_department | Ophthalmology
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | 243 Charles St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02461
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335230/suppl/GSM335230.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335230/suppl/GSM335230.dcp.gz
| | Sample_series_id | GSE12596
| | Sample_data_row_count | 31099
| | |
|
GSM335231 | GPL1355 |
|
RGC-3-OS
|
Retinal Ganglion Cells of Normal and Experimental Glaucoma Model of Brown Norway rats
|
Retinal Ganglion Cells isolated from adult male Brown Norway rats (300 to 450 g; Charles River, Boston, MA)
|
The NIH Neuroscience Microarray Consortium processed the GeneChip hybridization and scanning.
|
| Sample_geo_accession | GSM335231
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Oct 20 2008
| | Sample_last_update_date | Oct 01 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 = Retinal ganglion cells were bilaterally back-labeled by stereotaxic injection of Fluorogold into the superior colliculus. Sustained unilateral IOP elevation was produced by hypertonic saline episcleral vein injection in Brown Norway rats with the fellow eye serving as a control (n | 3). After IOP had been elevated for 10 days, the rats were killed by asphyxiation with carbon dioxide, the eyes were rapidly removed. The eyecups were collected and frozen in optimal cutting temperature compound and sectioned. Using laser capture microdissection, 2,000 retinal ganglion cells were captured from each eyes.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Isolated RGCs was processed for RNA extraction using the PicoPure RNA isolation kit (Arcturus, Mountain View, CA) according to the manufacturer’s protocol. Isolated RNA was amplified using the linear amplification as provided in the RiboAmp HS RNA amplification kit (Arcturus, Mountain View, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 10 µg of total RNA were used for cRNA labeling according to the recommended Affymetrix protocol.
| | Sample_hyb_protocol | The arrays were hybridized for 16h at 45°C in a rotisserie oven. After hybridization, arrays were washed using the Affymetrix fluidics station and stained with streptavidin-phycoerythrin according to the Affymetrix technical manual.
| | Sample_scan_protocol | Washed arrays were scanned on a Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | Scanned output files were visually inspected for hybridization artifacts and then analyzed by using Affymetrix GeneChip Operation Software (GCOS). Data obtained were analyzed with DChip.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Danyi,,Wang
| | Sample_contact_email | danyi_wang@meei.harvard.edu
| | Sample_contact_laboratory | Grosskreutz's lab
| | Sample_contact_department | Ophthalmology
| | Sample_contact_institute | Harvard Medical School
| | Sample_contact_address | 243 Charles St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02461
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335231/suppl/GSM335231.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM335nnn/GSM335231/suppl/GSM335231.dcp.gz
| | Sample_series_id | GSE12596
| | Sample_data_row_count | 31099
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