Search results for the GEO ID: GSE12618  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM315975 | GPL1261 |
|
mesenchyme1
|
bladder mesenchymal cells
|
gene reported: wild-type
strain: FVB/N
Sex: unknown
developmental stage: E13
theiler stage: 21
somite count: NA
developmental landmark: NA
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:10723
|
| Sample_geo_accession | GSM315975
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | Aug 29 2008
| | Sample_last_update_date | Dec 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | pool size: NA
| | Sample_treatment_protocol_ch1 | Pooled sample: no
| | Sample_treatment_protocol_ch1 | Dissection Method: Bladder microdissected and treated with 20 µM EDTA to dissociate the mesenchyme from the epithelium.
| | Sample_treatment_protocol_ch1 | FVB/N mice were time mated. At embryonic day 13 mice were euthanized by decapitation and the bladders were micro-dissected, cut just above the ureters and treated with 20 µM EDTA for 20 minutes. Mesenchymal and epithelial compartments were then separated by rimming the bladder with a needle and harvested in RLT. Total RNA was isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen:RNEASY
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_hyb_protocol | Amount labeled target hybridization to array: 10ug
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Analysis method: Affymetrix GCOS and Genespring and Avadis programs GCOS Tgt value: 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | GUDMAP,,Developers
| | Sample_contact_email | gudmap-editors@gudmap.org
| | Sample_contact_phone | +44 131 3322471
| | Sample_contact_laboratory | GUDMAP Database Group
| | Sample_contact_institute | MRC Human Genetics Unit
| | Sample_contact_address | Crewe Road
| | Sample_contact_city | Edinburgh
| | Sample_contact_zip/postal_code | EH4 2XU
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM315nnn/GSM315975/suppl/GSM315975.CEL.gz
| | Sample_series_id | GSE12618
| | Sample_data_row_count | 45101
| | |
|
GSM315976 | GPL1261 |
|
mesenchyme2
|
bladder mesenchymal cells
|
gene reported: wild-type
strain: FVB/N
Sex: unknown
developmental stage: E13
theiler stage: 21
somite count: NA
developmental landmark: NA
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:10724
|
| Sample_geo_accession | GSM315976
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | Aug 29 2008
| | Sample_last_update_date | Dec 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | pool size: NA
| | Sample_treatment_protocol_ch1 | Pooled sample: no
| | Sample_treatment_protocol_ch1 | Dissection Method: Bladder microdissected and treated with 20 µM EDTA to dissociate the mesenchyme from the epithelium.
| | Sample_treatment_protocol_ch1 | FVB/N mice were time mated. At embryonic day 13 mice were euthanized by decapitation and the bladders were micro-dissected, cut just above the ureters and treated with 20 µM EDTA for 20 minutes. Mesenchymal and epithelial compartments were then separated by rimming the bladder with a needle and harvested in RLT. Total RNA was isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen:RNEASY
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_hyb_protocol | Amount labeled target hybridization to array: 10ug
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Analysis method: Affymetrix GCOS and Genespring and Avadis programs GCOS Tgt value: 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | GUDMAP,,Developers
| | Sample_contact_email | gudmap-editors@gudmap.org
| | Sample_contact_phone | +44 131 3322471
| | Sample_contact_laboratory | GUDMAP Database Group
| | Sample_contact_institute | MRC Human Genetics Unit
| | Sample_contact_address | Crewe Road
| | Sample_contact_city | Edinburgh
| | Sample_contact_zip/postal_code | EH4 2XU
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM315nnn/GSM315976/suppl/GSM315976.CEL.gz
| | Sample_series_id | GSE12618
| | Sample_data_row_count | 45101
| | |
|
GSM315977 | GPL1261 |
|
mesenchyme4
|
bladder mesenchymal cells
|
gene reported: wild-type
strain: FVB/N
Sex: unknown
developmental stage: E13
theiler stage: 21
somite count: NA
developmental landmark: NA
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:10725
|
| Sample_geo_accession | GSM315977
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | Aug 29 2008
| | Sample_last_update_date | Dec 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | pool size: NA
| | Sample_treatment_protocol_ch1 | Pooled sample: no
| | Sample_treatment_protocol_ch1 | Dissection Method: Bladder microdissected and treated with 20 µM EDTA to dissociate the mesenchyme from the epithelium.
| | Sample_treatment_protocol_ch1 | FVB/N mice were time mated. At embryonic day 13 mice were euthanized by decapitation and the bladders were micro-dissected, cut just above the ureters and treated with 20 µM EDTA for 20 minutes. Mesenchymal and epithelial compartments were then separated by rimming the bladder with a needle and harvested in RLT. Total RNA was isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen:RNEASY
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_hyb_protocol | Amount labeled target hybridization to array: 10ug
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Analysis method: Affymetrix GCOS and Genespring and Avadis programs GCOS Tgt value: 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | GUDMAP,,Developers
| | Sample_contact_email | gudmap-editors@gudmap.org
| | Sample_contact_phone | +44 131 3322471
| | Sample_contact_laboratory | GUDMAP Database Group
| | Sample_contact_institute | MRC Human Genetics Unit
| | Sample_contact_address | Crewe Road
| | Sample_contact_city | Edinburgh
| | Sample_contact_zip/postal_code | EH4 2XU
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM315nnn/GSM315977/suppl/GSM315977.CEL.gz
| | Sample_series_id | GSE12618
| | Sample_data_row_count | 45101
| | |
|
GSM315978 | GPL1261 |
|
urothelium5
|
bladder epithelial cells
|
gene reported: wild-type
strain: FVB/N
Sex: unknown
developmental stage: E13
theiler stage: 21
somite count: NA
developmental landmark: NA
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:10726
|
| Sample_geo_accession | GSM315978
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | Aug 29 2008
| | Sample_last_update_date | Dec 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | pool size: NA
| | Sample_treatment_protocol_ch1 | Pooled sample: no
| | Sample_treatment_protocol_ch1 | Dissection Method: Bladder microdissected and treated with 20 µM EDTA to dissociate the mesenchyme from the epithelium.
| | Sample_treatment_protocol_ch1 | FVB/N mice were time mated. At embryonic day 13 mice were euthanized by decapitation and the bladders were micro-dissected, cut just above the ureters and treated with 20 µM EDTA for 20 minutes. Mesenchymal and epithelial compartments were then separated by rimming the bladder with a needle and harvested in RLT. Total RNA was isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen:RNEASY
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_hyb_protocol | Amount labeled target hybridization to array: 10ug
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Analysis method: Affymetrix GCOS and Genespring and Avadis programs GCOS Tgt value: 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | GUDMAP,,Developers
| | Sample_contact_email | gudmap-editors@gudmap.org
| | Sample_contact_phone | +44 131 3322471
| | Sample_contact_laboratory | GUDMAP Database Group
| | Sample_contact_institute | MRC Human Genetics Unit
| | Sample_contact_address | Crewe Road
| | Sample_contact_city | Edinburgh
| | Sample_contact_zip/postal_code | EH4 2XU
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM315nnn/GSM315978/suppl/GSM315978.CEL.gz
| | Sample_series_id | GSE12618
| | Sample_data_row_count | 45101
| | |
|
GSM315979 | GPL1261 |
|
urothelium7
|
bladder epithelial cells
|
gene reported: wild-type
strain: FVB/N
Sex: unknown
developmental stage: E13
theiler stage: 21
somite count: NA
developmental landmark: NA
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:10727
|
| Sample_geo_accession | GSM315979
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | Aug 29 2008
| | Sample_last_update_date | Dec 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | pool size: NA
| | Sample_treatment_protocol_ch1 | Pooled sample: no
| | Sample_treatment_protocol_ch1 | Dissection Method: Bladder microdissected and treated with 20 µM EDTA to dissociate the mesenchyme from the epithelium.
| | Sample_treatment_protocol_ch1 | FVB/N mice were time mated. At embryonic day 13 mice were euthanized by decapitation and the bladders were micro-dissected, cut just above the ureters and treated with 20 µM EDTA for 20 minutes. Mesenchymal and epithelial compartments were then separated by rimming the bladder with a needle and harvested in RLT. Total RNA was isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen:RNEASY
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_hyb_protocol | Amount labeled target hybridization to array: 10ug
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Analysis method: Affymetrix GCOS and Genespring and Avadis programs GCOS Tgt value: 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | GUDMAP,,Developers
| | Sample_contact_email | gudmap-editors@gudmap.org
| | Sample_contact_phone | +44 131 3322471
| | Sample_contact_laboratory | GUDMAP Database Group
| | Sample_contact_institute | MRC Human Genetics Unit
| | Sample_contact_address | Crewe Road
| | Sample_contact_city | Edinburgh
| | Sample_contact_zip/postal_code | EH4 2XU
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM315nnn/GSM315979/suppl/GSM315979.CEL.gz
| | Sample_series_id | GSE12618
| | Sample_data_row_count | 45101
| | |
|
GSM315980 | GPL1261 |
|
urothelium8
|
bladder epithelial cells
|
gene reported: wild-type
strain: FVB/N
Sex: unknown
developmental stage: E13
theiler stage: 21
somite count: NA
developmental landmark: NA
|
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter Protocols
Rounds of amplification: 2
URL: http://www.gudmap.org/gudmap/pages/mic_submission.html?id=GUDMAP:10728
|
| Sample_geo_accession | GSM315980
| | Sample_status | Public on Jan 16 2009
| | Sample_submission_date | Aug 29 2008
| | Sample_last_update_date | Dec 29 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | pool size: NA
| | Sample_treatment_protocol_ch1 | Pooled sample: no
| | Sample_treatment_protocol_ch1 | Dissection Method: Bladder microdissected and treated with 20 µM EDTA to dissociate the mesenchyme from the epithelium.
| | Sample_treatment_protocol_ch1 | FVB/N mice were time mated. At embryonic day 13 mice were euthanized by decapitation and the bladders were micro-dissected, cut just above the ureters and treated with 20 µM EDTA for 20 minutes. Mesenchymal and epithelial compartments were then separated by rimming the bladder with a needle and harvested in RLT. Total RNA was isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen:RNEASY
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-X-X-NHS (EPICENTRE Biotechnologies)
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_hyb_protocol | Amount labeled target hybridization to array: 10ug
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | Analysis method: Affymetrix GCOS and Genespring and Avadis programs GCOS Tgt value: 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | GUDMAP,,Developers
| | Sample_contact_email | gudmap-editors@gudmap.org
| | Sample_contact_phone | +44 131 3322471
| | Sample_contact_laboratory | GUDMAP Database Group
| | Sample_contact_institute | MRC Human Genetics Unit
| | Sample_contact_address | Crewe Road
| | Sample_contact_city | Edinburgh
| | Sample_contact_zip/postal_code | EH4 2XU
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM315nnn/GSM315980/suppl/GSM315980.CEL.gz
| | Sample_series_id | GSE12618
| | Sample_data_row_count | 45101
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