Search results for the GEO ID: GSE12710  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM318889 | GPL570 |
|
Direct draw, Tempus tube, replicate R (Blood_direct_ABI_R)
|
Peripheral blood
|
n/a
|
direct draw, Tempus tube, replicate R
|
| Sample_geo_accession | GSM318889
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Sep 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318889/suppl/GSM318889.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
GSM318890 | GPL570 |
|
Direct draw, PAXgene tube, replicate R (Blood_direct_PAX_R)
|
Peripheral blood
|
n/a
|
direct draw, PAXgene tube, replicate R
|
| Sample_geo_accession | GSM318890
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Nov 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318890/suppl/GSM318890.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
GSM318891 | GPL570 |
|
Heparin, Tempus tube, replicate R (Blood_Heparin_ABI_R)
|
Peripheral blood
|
n/a
|
Heparin, Tempus tube, replicate R
|
| Sample_geo_accession | GSM318891
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Sep 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318891/suppl/GSM318891.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
GSM318892 | GPL570 |
|
Heparin, PAXgene tube, replicate R (Blood_Heparin_PAX_R)
|
Peripheral blood
|
n/a
|
Heparin, PAXgene tube, replicate R
|
| Sample_geo_accession | GSM318892
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Sep 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318892/suppl/GSM318892.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
GSM318893 | GPL570 |
|
Direct draw, Tempus tube, replicate S (Blood_direct_ABI_S)
|
Peripheral blood
|
n/a
|
direct draw, Tempus tube, replicate S
|
| Sample_geo_accession | GSM318893
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Sep 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318893/suppl/GSM318893.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
GSM318894 | GPL570 |
|
Direct draw, PAXgene tube, replicate S (Blood_direct_PAX_S)
|
Peripheral blood
|
n/a
|
direct draw, PAXgene tube, replicate S
|
| Sample_geo_accession | GSM318894
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Sep 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318894/suppl/GSM318894.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
GSM318895 | GPL570 |
|
Heparin, Tempus tube, replicate S (Blood_Heparin_ABI_S)
|
Peripheral blood
|
n/a
|
Heparin, Tempus tube, replicate S
|
| Sample_geo_accession | GSM318895
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Sep 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318895/suppl/GSM318895.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
GSM318896 | GPL570 |
|
Heparin, PAXgene tube, replicate S (Blood_Heparin_PAX_S)
|
Peripheral blood
|
n/a
|
Heparin, PAXgene tube, replicate S
|
| Sample_geo_accession | GSM318896
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Sep 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318896/suppl/GSM318896.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
GSM318897 | GPL570 |
|
Direct draw, Tempus tube, replicate T (Blood_direct_ABI_T)
|
Peripheral blood
|
n/a
|
direct draw, Tempus tube, replicate T
|
| Sample_geo_accession | GSM318897
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Sep 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318897/suppl/GSM318897.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
GSM318898 | GPL570 |
|
Direct draw, PAXgene tube, replicate T (Blood_direct_PAX_T)
|
Peripheral blood
|
n/a
|
direct draw, PAXgene tube, replicate T
|
| Sample_geo_accession | GSM318898
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Nov 07 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318898/suppl/GSM318898.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
GSM318899 | GPL570 |
|
Heparin, Tempus tube, replicate T (Blood_Heparin_ABI_T)
|
Peripheral blood
|
n/a
|
Heparin, Tempus tube, replicate T
|
| Sample_geo_accession | GSM318899
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Sep 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318899/suppl/GSM318899.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
GSM318900 | GPL570 |
|
Heparin, PAXgene tube, replicate T (Blood_Heparin_PAX_T)
|
Peripheral blood
|
n/a
|
Heparin, PAXgene tube, replicate T
|
| Sample_geo_accession | GSM318900
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Sep 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318900/suppl/GSM318900.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
GSM318901 | GPL570 |
|
Direct draw, Tempus tube, replicate U (Blood_direct_ABI_U)
|
Peripheral blood
|
n/a
|
direct draw, Tempus tube, replicate U
|
| Sample_geo_accession | GSM318901
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Sep 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318901/suppl/GSM318901.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
GSM318902 | GPL570 |
|
Direct draw, PAXgene tube, replicate U (Blood_direct_PAX_U)
|
Peripheral blood
|
n/a
|
direct draw, PAXgene tube, replicate U
|
| Sample_geo_accession | GSM318902
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Sep 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318902/suppl/GSM318902.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
GSM318903 | GPL570 |
|
Heparin, Tempus tube, replicate U (Blood_Heparin_ABI_U)
|
Peripheral blood
|
n/a
|
Heparin, Tempus tube, replicate U
|
| Sample_geo_accession | GSM318903
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Sep 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318903/suppl/GSM318903.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
GSM318904 | GPL570 |
|
Heparin, PAXgene tube, replicate U (Blood_Heparin_PAX_U)
|
Peripheral blood
|
n/a
|
Heparin, PAXgene tube, replicate U
|
| Sample_geo_accession | GSM318904
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Sep 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318904/suppl/GSM318904.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
GSM318905 | GPL570 |
|
Direct draw, Tempus tube, replicate Y (Blood_direct_ABI_Y)
|
Peripheral blood
|
n/a
|
direct draw, Tempus tube, replicate Y
|
| Sample_geo_accession | GSM318905
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Sep 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318905/suppl/GSM318905.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
GSM318906 | GPL570 |
|
Direct draw, PAXgene tube, replicate Y (Blood_direct_PAX_Y)
|
Peripheral blood
|
n/a
|
direct draw, PAXgene tube, replicate Y
|
| Sample_geo_accession | GSM318906
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Sep 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318906/suppl/GSM318906.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
GSM318907 | GPL570 |
|
Heparin, Tempus tube, replicate Y (Blood_Heparin_ABI_Y)
|
Peripheral blood
|
n/a
|
Heparin, Tempus tube, replicate Y
|
| Sample_geo_accession | GSM318907
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Sep 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318907/suppl/GSM318907.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
GSM318908 | GPL570 |
|
Heparin, PAXgene tube, replicate Y (Blood_Heparin_PAX_Y)
|
Peripheral blood
|
n/a
|
Heparin, PAXgene tube, replicate Y
|
| Sample_geo_accession | GSM318908
| | Sample_status | Public on Oct 30 2008
| | Sample_submission_date | Sep 09 2008
| | Sample_last_update_date | Sep 09 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Whole blood from 5 healthy individuals was drawn directly in PAXgene or Tempus tubes. The same 5 healthy control samples were also drawn in Li Heparin tubes with no PHA stimulation
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted at the ITN Central Nucleic Acid Isolation Core facility, Pittsburgh, PA according to the ITN modified method for Tempus. Whole blood samples collected into Tempus vacuette were extracted using ABI Prism 6100 Nucleic Acid PrepStation and using Tempus extraction reagents. The PAXgene whole blood sample were processed using the PAXgene Blood RNA Kit based on the Qiagen method for column purification of nucleic acid.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol. cRNA target amplificationwas performed using the Affymetrix In-vitro Transcription (IVT) Kit followed by a Globin Reduction step.
| | Sample_hyb_protocol | Hybridization on Affymetrix HG-U133 2.0 plus array according to manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data normalizaion was performed using Bioconductor package THREESTEP(background.method="MASIM", noemalize.method="scaling", summary.method="tukey.biweight").
| | Sample_platform_id | GPL570
| | Sample_contact_name | Zhong,,Gao
| | Sample_contact_institute | Johns Hopkins University
| | Sample_contact_address | Ross 844 720 Rutland Avenue
| | Sample_contact_city | Baltimore
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21205
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM318nnn/GSM318908/suppl/GSM318908.CEL.gz
| | Sample_series_id | GSE12710
| | Sample_series_id | GSE12711
| | Sample_data_row_count | 54675
| | |
|
| |
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