Search results for the GEO ID: GSE12734  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM319505 | GPL570 |
|
CLL patient ZAP70 high, biological rep1
|
CD19+ purified cells from CLL patient ZAP70 high, biological rep1
|
Sex:F
Age:69
Binet Stage:A
Mutational status: UM
ZAP70 +
LPL +
CD38 +
|
Gene expression data from CLL patient expressing high level of ZAP70 measured by real time PCR
|
| Sample_geo_accession | GSM319505
| | Sample_status | Public on Apr 18 2009
| | Sample_submission_date | Sep 10 2008
| | Sample_last_update_date | Apr 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Peripheral Blood Mononuclear Cells (PBMC) were obtained from blood of Chronic lymphocytic leukemia patients.
| | Sample_extract_protocol_ch1 | PBMC were isolated by density-gradient centrifugation and B cells were purified with a CD19+ magnetic-bead system.
| | Sample_extract_protocol_ch1 | Total RNA was extracted from purified CD19+ cells in a single step using TriPure Isolation Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1.5 µg total RNA (www.affymetrix.com)
| | Sample_hyb_protocol | Following fragmentation, 16 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix gENESHIP Fluidics Station 450 regarding Affymetrix protocol (www.affymetrix.com)
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Geneship Scanner 3000 7G
| | Sample_data_processing | Image analysis and probe quantification were done with Affymetrix software, which produced raw probe intensity data in the Affymetrix CEL files. Normalization was done with the RMA method, which processed a group of CEL files simultaneously using BRB arrays tools.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Basile,,Stamatopoulos
| | Sample_contact_department | Experimental Hematology
| | Sample_contact_institute | Jules Bordet Institute
| | Sample_contact_address | Bd de Waterloo n°121
| | Sample_contact_city | Bruxelles
| | Sample_contact_zip/postal_code | 1000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319505/suppl/GSM319505.CEL.gz
| | Sample_series_id | GSE12734
| | Sample_data_row_count | 54675
| | |
|
GSM319506 | GPL570 |
|
CLL patient ZAP70 high, biological rep2
|
CD19+ purified cells from CLL patient ZAP70 high, biological rep2
|
Sex:M
Age:68
Binet Stage:C
Mutational status: UM
ZAP70 +
LPL +
CD38 +
|
Gene expression data from CLL patient expressing high level of ZAP70 measured by real time PCR
|
| Sample_geo_accession | GSM319506
| | Sample_status | Public on Apr 18 2009
| | Sample_submission_date | Sep 10 2008
| | Sample_last_update_date | Apr 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Peripheral Blood Mononuclear Cells (PBMC) were obtained from blood of Chronic lymphocytic leukemia patients.
| | Sample_extract_protocol_ch1 | PBMC were isolated by density-gradient centrifugation and B cells were purified with a CD19+ magnetic-bead system.
| | Sample_extract_protocol_ch1 | Total RNA was extracted from purified CD19+ cells in a single step using TriPure Isolation Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1.5 µg total RNA (www.affymetrix.com)
| | Sample_hyb_protocol | Following fragmentation, 16 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix gENESHIP Fluidics Station 450 regarding Affymetrix protocol (www.affymetrix.com)
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Geneship Scanner 3000 7G
| | Sample_data_processing | Image analysis and probe quantification were done with Affymetrix software, which produced raw probe intensity data in the Affymetrix CEL files. Normalization was done with the RMA method, which processed a group of CEL files simultaneously using BRB arrays tools.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Basile,,Stamatopoulos
| | Sample_contact_department | Experimental Hematology
| | Sample_contact_institute | Jules Bordet Institute
| | Sample_contact_address | Bd de Waterloo n°121
| | Sample_contact_city | Bruxelles
| | Sample_contact_zip/postal_code | 1000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319506/suppl/GSM319506.CEL.gz
| | Sample_series_id | GSE12734
| | Sample_data_row_count | 54675
| | |
|
GSM319507 | GPL570 |
|
CLL patient ZAP70 high, biological rep3
|
CD19+ purified cells from CLL patient ZAP70 high, biological rep3
|
Sex:F
Age:57
Binet Stage:B
Mutational status: UM
ZAP70 +
LPL -
CD38 +
|
Gene expression data from CLL patient expressing high level of ZAP70 measured by real time PCR
|
| Sample_geo_accession | GSM319507
| | Sample_status | Public on Apr 18 2009
| | Sample_submission_date | Sep 10 2008
| | Sample_last_update_date | Apr 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Peripheral Blood Mononuclear Cells (PBMC) were obtained from blood of Chronic lymphocytic leukemia patients.
| | Sample_extract_protocol_ch1 | PBMC were isolated by density-gradient centrifugation and B cells were purified with a CD19+ magnetic-bead system.
| | Sample_extract_protocol_ch1 | Total RNA was extracted from purified CD19+ cells in a single step using TriPure Isolation Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1.5 µg total RNA (www.affymetrix.com)
| | Sample_hyb_protocol | Following fragmentation, 16 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix gENESHIP Fluidics Station 450 regarding Affymetrix protocol (www.affymetrix.com)
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Geneship Scanner 3000 7G
| | Sample_data_processing | Image analysis and probe quantification were done with Affymetrix software, which produced raw probe intensity data in the Affymetrix CEL files. Normalization was done with the RMA method, which processed a group of CEL files simultaneously using BRB arrays tools.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Basile,,Stamatopoulos
| | Sample_contact_department | Experimental Hematology
| | Sample_contact_institute | Jules Bordet Institute
| | Sample_contact_address | Bd de Waterloo n°121
| | Sample_contact_city | Bruxelles
| | Sample_contact_zip/postal_code | 1000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319507/suppl/GSM319507.CEL.gz
| | Sample_series_id | GSE12734
| | Sample_data_row_count | 54675
| | |
|
GSM319508 | GPL570 |
|
CLL patient ZAP70 high, biological rep4
|
CD19+ purified cells from CLL patient ZAP70 high, biological rep4
|
Sex:F
Age:67
Binet Stage:A
Mutational status: M
ZAP70 +
LPL -
CD38 +
|
Gene expression data from CLL patient expressing high level of ZAP70 measured by real time PCR
|
| Sample_geo_accession | GSM319508
| | Sample_status | Public on Apr 18 2009
| | Sample_submission_date | Sep 10 2008
| | Sample_last_update_date | Apr 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Peripheral Blood Mononuclear Cells (PBMC) were obtained from blood of Chronic lymphocytic leukemia patients.
| | Sample_extract_protocol_ch1 | PBMC were isolated by density-gradient centrifugation and B cells were purified with a CD19+ magnetic-bead system.
| | Sample_extract_protocol_ch1 | Total RNA was extracted from purified CD19+ cells in a single step using TriPure Isolation Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1.5 µg total RNA (www.affymetrix.com)
| | Sample_hyb_protocol | Following fragmentation, 16 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix gENESHIP Fluidics Station 450 regarding Affymetrix protocol (www.affymetrix.com)
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Geneship Scanner 3000 7G
| | Sample_data_processing | Image analysis and probe quantification were done with Affymetrix software, which produced raw probe intensity data in the Affymetrix CEL files. Normalization was done with the RMA method, which processed a group of CEL files simultaneously using BRB arrays tools.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Basile,,Stamatopoulos
| | Sample_contact_department | Experimental Hematology
| | Sample_contact_institute | Jules Bordet Institute
| | Sample_contact_address | Bd de Waterloo n°121
| | Sample_contact_city | Bruxelles
| | Sample_contact_zip/postal_code | 1000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319508/suppl/GSM319508.CEL.gz
| | Sample_series_id | GSE12734
| | Sample_data_row_count | 54675
| | |
|
GSM319509 | GPL570 |
|
CLL patient ZAP70 high, biological rep5
|
CD19+ purified cells from CLL patient ZAP70 high, biological rep5
|
Sex:M
Age:61
Binet Stage:C
Mutational status: UM
ZAP70 +
LPL +
CD38 +
|
Gene expression data from CLL patient expressing high level of ZAP70 measured by real time PCR
|
| Sample_geo_accession | GSM319509
| | Sample_status | Public on Apr 18 2009
| | Sample_submission_date | Sep 10 2008
| | Sample_last_update_date | Apr 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Peripheral Blood Mononuclear Cells (PBMC) were obtained from blood of Chronic lymphocytic leukemia patients.
| | Sample_extract_protocol_ch1 | PBMC were isolated by density-gradient centrifugation and B cells were purified with a CD19+ magnetic-bead system.
| | Sample_extract_protocol_ch1 | Total RNA was extracted from purified CD19+ cells in a single step using TriPure Isolation Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1.5 µg total RNA (www.affymetrix.com)
| | Sample_hyb_protocol | Following fragmentation, 16 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix gENESHIP Fluidics Station 450 regarding Affymetrix protocol (www.affymetrix.com)
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Geneship Scanner 3000 7G
| | Sample_data_processing | Image analysis and probe quantification were done with Affymetrix software, which produced raw probe intensity data in the Affymetrix CEL files. Normalization was done with the RMA method, which processed a group of CEL files simultaneously using BRB arrays tools.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Basile,,Stamatopoulos
| | Sample_contact_department | Experimental Hematology
| | Sample_contact_institute | Jules Bordet Institute
| | Sample_contact_address | Bd de Waterloo n°121
| | Sample_contact_city | Bruxelles
| | Sample_contact_zip/postal_code | 1000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319509/suppl/GSM319509.CEL.gz
| | Sample_series_id | GSE12734
| | Sample_data_row_count | 54675
| | |
|
GSM319510 | GPL570 |
|
CLL patient ZAP70 high, biological rep6
|
CD19+ purified cells from CLL patient ZAP70 high, biological rep6
|
Sex:F
Age:74
Binet Stage:C
Mutational status: UM
ZAP70 +
LPL +
CD38 +
|
Gene expression data from CLL patient expressing high level of ZAP70 measured by real time PCR
|
| Sample_geo_accession | GSM319510
| | Sample_status | Public on Apr 18 2009
| | Sample_submission_date | Sep 10 2008
| | Sample_last_update_date | Apr 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Peripheral Blood Mononuclear Cells (PBMC) were obtained from blood of Chronic lymphocytic leukemia patients.
| | Sample_extract_protocol_ch1 | PBMC were isolated by density-gradient centrifugation and B cells were purified with a CD19+ magnetic-bead system.
| | Sample_extract_protocol_ch1 | Total RNA was extracted from purified CD19+ cells in a single step using TriPure Isolation Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1.5 µg total RNA (www.affymetrix.com)
| | Sample_hyb_protocol | Following fragmentation, 16 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix gENESHIP Fluidics Station 450 regarding Affymetrix protocol (www.affymetrix.com)
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Geneship Scanner 3000 7G
| | Sample_data_processing | Image analysis and probe quantification were done with Affymetrix software, which produced raw probe intensity data in the Affymetrix CEL files. Normalization was done with the RMA method, which processed a group of CEL files simultaneously using BRB arrays tools.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Basile,,Stamatopoulos
| | Sample_contact_department | Experimental Hematology
| | Sample_contact_institute | Jules Bordet Institute
| | Sample_contact_address | Bd de Waterloo n°121
| | Sample_contact_city | Bruxelles
| | Sample_contact_zip/postal_code | 1000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319510/suppl/GSM319510.CEL.gz
| | Sample_series_id | GSE12734
| | Sample_data_row_count | 54675
| | |
|
GSM319511 | GPL570 |
|
CLL patient ZAP70 high, biological rep7
|
CD19+ purified cells from CLL patient ZAP70 high, biological rep7
|
Sex:M
Age:73
Binet Stage:B
Mutational status: UM
ZAP70 +
LPL +
CD38 +
|
Gene expression data from CLL patient expressing high level of ZAP70 measured by real time PCR
|
| Sample_geo_accession | GSM319511
| | Sample_status | Public on Apr 18 2009
| | Sample_submission_date | Sep 10 2008
| | Sample_last_update_date | Apr 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Peripheral Blood Mononuclear Cells (PBMC) were obtained from blood of Chronic lymphocytic leukemia patients.
| | Sample_extract_protocol_ch1 | PBMC were isolated by density-gradient centrifugation and B cells were purified with a CD19+ magnetic-bead system.
| | Sample_extract_protocol_ch1 | Total RNA was extracted from purified CD19+ cells in a single step using TriPure Isolation Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1.5 µg total RNA (www.affymetrix.com)
| | Sample_hyb_protocol | Following fragmentation, 16 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix gENESHIP Fluidics Station 450 regarding Affymetrix protocol (www.affymetrix.com)
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Geneship Scanner 3000 7G
| | Sample_data_processing | Image analysis and probe quantification were done with Affymetrix software, which produced raw probe intensity data in the Affymetrix CEL files. Normalization was done with the RMA method, which processed a group of CEL files simultaneously using BRB arrays tools.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Basile,,Stamatopoulos
| | Sample_contact_department | Experimental Hematology
| | Sample_contact_institute | Jules Bordet Institute
| | Sample_contact_address | Bd de Waterloo n°121
| | Sample_contact_city | Bruxelles
| | Sample_contact_zip/postal_code | 1000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319511/suppl/GSM319511.CEL.gz
| | Sample_series_id | GSE12734
| | Sample_data_row_count | 54675
| | |
|
GSM319512 | GPL570 |
|
CLL patient ZAP70 low, biological rep1
|
CD19+ purified cells from CLL patient ZAP70 low, biological rep1
|
Sex:F
Age:67
Binet Stage:A
Mutational status: M
ZAP70 -
LPL -
CD38 +
|
Gene expression data from CLL patient expressing low level of ZAP70 measured by real time PCR
|
| Sample_geo_accession | GSM319512
| | Sample_status | Public on Apr 18 2009
| | Sample_submission_date | Sep 10 2008
| | Sample_last_update_date | Apr 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Peripheral Blood Mononuclear Cells (PBMC) were obtained from blood of Chronic lymphocytic leukemia patients.
| | Sample_extract_protocol_ch1 | PBMC were isolated by density-gradient centrifugation and B cells were purified with a CD19+ magnetic-bead system.
| | Sample_extract_protocol_ch1 | Total RNA was extracted from purified CD19+ cells in a single step using TriPure Isolation Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1.5 µg total RNA (www.affymetrix.com)
| | Sample_hyb_protocol | Following fragmentation, 16 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix gENESHIP Fluidics Station 450 regarding Affymetrix protocol (www.affymetrix.com)
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Geneship Scanner 3000 7G
| | Sample_data_processing | Image analysis and probe quantification were done with Affymetrix software, which produced raw probe intensity data in the Affymetrix CEL files. Normalization was done with the RMA method, which processed a group of CEL files simultaneously using BRB arrays tools.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Basile,,Stamatopoulos
| | Sample_contact_department | Experimental Hematology
| | Sample_contact_institute | Jules Bordet Institute
| | Sample_contact_address | Bd de Waterloo n°121
| | Sample_contact_city | Bruxelles
| | Sample_contact_zip/postal_code | 1000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319512/suppl/GSM319512.CEL.gz
| | Sample_series_id | GSE12734
| | Sample_data_row_count | 54675
| | |
|
GSM319513 | GPL570 |
|
CLL patient ZAP70 low, biological rep2
|
CD19+ purified cells from CLL patient ZAP70 low, biological rep2
|
Sex:F
Age:68
Binet Stage:A
Mutational status: M
ZAP70 -
LPL -
CD38 -
|
Gene expression data from CLL patient expressing low level of ZAP70 measured by real time PCR
|
| Sample_geo_accession | GSM319513
| | Sample_status | Public on Apr 18 2009
| | Sample_submission_date | Sep 10 2008
| | Sample_last_update_date | Apr 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Peripheral Blood Mononuclear Cells (PBMC) were obtained from blood of Chronic lymphocytic leukemia patients.
| | Sample_extract_protocol_ch1 | PBMC were isolated by density-gradient centrifugation and B cells were purified with a CD19+ magnetic-bead system.
| | Sample_extract_protocol_ch1 | Total RNA was extracted from purified CD19+ cells in a single step using TriPure Isolation Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1.5 µg total RNA (www.affymetrix.com)
| | Sample_hyb_protocol | Following fragmentation, 16 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix gENESHIP Fluidics Station 450 regarding Affymetrix protocol (www.affymetrix.com)
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Geneship Scanner 3000 7G
| | Sample_data_processing | Image analysis and probe quantification were done with Affymetrix software, which produced raw probe intensity data in the Affymetrix CEL files. Normalization was done with the RMA method, which processed a group of CEL files simultaneously using BRB arrays tools.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Basile,,Stamatopoulos
| | Sample_contact_department | Experimental Hematology
| | Sample_contact_institute | Jules Bordet Institute
| | Sample_contact_address | Bd de Waterloo n°121
| | Sample_contact_city | Bruxelles
| | Sample_contact_zip/postal_code | 1000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319513/suppl/GSM319513.CEL.gz
| | Sample_series_id | GSE12734
| | Sample_data_row_count | 54675
| | |
|
GSM319514 | GPL570 |
|
CLL patient ZAP70 low, biological rep3
|
CD19+ purified cells from CLL patient ZAP70 low, biological rep3
|
Sex:F
Age:59
Binet Stage:B
Mutational status: M
ZAP70 -
LPL -
CD38 -
|
Gene expression data from CLL patient expressing low level of ZAP70 measured by real time PCR
|
| Sample_geo_accession | GSM319514
| | Sample_status | Public on Apr 18 2009
| | Sample_submission_date | Sep 10 2008
| | Sample_last_update_date | Apr 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Peripheral Blood Mononuclear Cells (PBMC) were obtained from blood of Chronic lymphocytic leukemia patients.
| | Sample_extract_protocol_ch1 | PBMC were isolated by density-gradient centrifugation and B cells were purified with a CD19+ magnetic-bead system.
| | Sample_extract_protocol_ch1 | Total RNA was extracted from purified CD19+ cells in a single step using TriPure Isolation Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1.5 µg total RNA (www.affymetrix.com)
| | Sample_hyb_protocol | Following fragmentation, 16 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix gENESHIP Fluidics Station 450 regarding Affymetrix protocol (www.affymetrix.com)
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Geneship Scanner 3000 7G
| | Sample_data_processing | Image analysis and probe quantification were done with Affymetrix software, which produced raw probe intensity data in the Affymetrix CEL files. Normalization was done with the RMA method, which processed a group of CEL files simultaneously using BRB arrays tools.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Basile,,Stamatopoulos
| | Sample_contact_department | Experimental Hematology
| | Sample_contact_institute | Jules Bordet Institute
| | Sample_contact_address | Bd de Waterloo n°121
| | Sample_contact_city | Bruxelles
| | Sample_contact_zip/postal_code | 1000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319514/suppl/GSM319514.CEL.gz
| | Sample_series_id | GSE12734
| | Sample_data_row_count | 54675
| | |
|
GSM319515 | GPL570 |
|
CLL patient ZAP70 low, biological rep4
|
CD19+ purified cells from CLL patient ZAP70 low, biological rep4
|
Sex:M
Age:67
Binet Stage:B
Mutational status: M
ZAP70 -
LPL +
CD38 +
|
Gene expression data from CLL patient expressing low level of ZAP70 measured by real time PCR
|
| Sample_geo_accession | GSM319515
| | Sample_status | Public on Apr 18 2009
| | Sample_submission_date | Sep 10 2008
| | Sample_last_update_date | Apr 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Peripheral Blood Mononuclear Cells (PBMC) were obtained from blood of Chronic lymphocytic leukemia patients.
| | Sample_extract_protocol_ch1 | PBMC were isolated by density-gradient centrifugation and B cells were purified with a CD19+ magnetic-bead system.
| | Sample_extract_protocol_ch1 | Total RNA was extracted from purified CD19+ cells in a single step using TriPure Isolation Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1.5 µg total RNA (www.affymetrix.com)
| | Sample_hyb_protocol | Following fragmentation, 16 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix gENESHIP Fluidics Station 450 regarding Affymetrix protocol (www.affymetrix.com)
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Geneship Scanner 3000 7G
| | Sample_data_processing | Image analysis and probe quantification were done with Affymetrix software, which produced raw probe intensity data in the Affymetrix CEL files. Normalization was done with the RMA method, which processed a group of CEL files simultaneously using BRB arrays tools.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Basile,,Stamatopoulos
| | Sample_contact_department | Experimental Hematology
| | Sample_contact_institute | Jules Bordet Institute
| | Sample_contact_address | Bd de Waterloo n°121
| | Sample_contact_city | Bruxelles
| | Sample_contact_zip/postal_code | 1000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319515/suppl/GSM319515.CEL.gz
| | Sample_series_id | GSE12734
| | Sample_data_row_count | 54675
| | |
|
GSM319516 | GPL570 |
|
CLL patient ZAP70 low, biological rep5
|
CD19+ purified cells from CLL patient ZAP70 low, biological rep5
|
Sex:M
Age:72
Binet Stage:B
Mutational status: M
ZAP70 -
LPL -
CD38 -
|
Gene expression data from CLL patient expressing low level of ZAP70 measured by real time PCR
|
| Sample_geo_accession | GSM319516
| | Sample_status | Public on Apr 18 2009
| | Sample_submission_date | Sep 10 2008
| | Sample_last_update_date | Apr 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Peripheral Blood Mononuclear Cells (PBMC) were obtained from blood of Chronic lymphocytic leukemia patients.
| | Sample_extract_protocol_ch1 | PBMC were isolated by density-gradient centrifugation and B cells were purified with a CD19+ magnetic-bead system.
| | Sample_extract_protocol_ch1 | Total RNA was extracted from purified CD19+ cells in a single step using TriPure Isolation Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1.5 µg total RNA (www.affymetrix.com)
| | Sample_hyb_protocol | Following fragmentation, 16 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix gENESHIP Fluidics Station 450 regarding Affymetrix protocol (www.affymetrix.com)
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Geneship Scanner 3000 7G
| | Sample_data_processing | Image analysis and probe quantification were done with Affymetrix software, which produced raw probe intensity data in the Affymetrix CEL files. Normalization was done with the RMA method, which processed a group of CEL files simultaneously using BRB arrays tools.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Basile,,Stamatopoulos
| | Sample_contact_department | Experimental Hematology
| | Sample_contact_institute | Jules Bordet Institute
| | Sample_contact_address | Bd de Waterloo n°121
| | Sample_contact_city | Bruxelles
| | Sample_contact_zip/postal_code | 1000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319516/suppl/GSM319516.CEL.gz
| | Sample_series_id | GSE12734
| | Sample_data_row_count | 54675
| | |
|
GSM319517 | GPL570 |
|
CLL patient ZAP70 low, biological rep6
|
CD19+ purified cells from CLL patient ZAP70 low, biological rep6
|
Sex:M
Age:77
Binet Stage:A
Mutational status: M
ZAP70 -
LPL -
CD38 +
|
Gene expression data from CLL patient expressing low level of ZAP70 measured by real time PCR
|
| Sample_geo_accession | GSM319517
| | Sample_status | Public on Apr 18 2009
| | Sample_submission_date | Sep 10 2008
| | Sample_last_update_date | Apr 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Peripheral Blood Mononuclear Cells (PBMC) were obtained from blood of Chronic lymphocytic leukemia patients.
| | Sample_extract_protocol_ch1 | PBMC were isolated by density-gradient centrifugation and B cells were purified with a CD19+ magnetic-bead system.
| | Sample_extract_protocol_ch1 | Total RNA was extracted from purified CD19+ cells in a single step using TriPure Isolation Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1.5 µg total RNA (www.affymetrix.com)
| | Sample_hyb_protocol | Following fragmentation, 16 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix gENESHIP Fluidics Station 450 regarding Affymetrix protocol (www.affymetrix.com)
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Geneship Scanner 3000 7G
| | Sample_data_processing | Image analysis and probe quantification were done with Affymetrix software, which produced raw probe intensity data in the Affymetrix CEL files. Normalization was done with the RMA method, which processed a group of CEL files simultaneously using BRB arrays tools.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Basile,,Stamatopoulos
| | Sample_contact_department | Experimental Hematology
| | Sample_contact_institute | Jules Bordet Institute
| | Sample_contact_address | Bd de Waterloo n°121
| | Sample_contact_city | Bruxelles
| | Sample_contact_zip/postal_code | 1000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319517/suppl/GSM319517.CEL.gz
| | Sample_series_id | GSE12734
| | Sample_data_row_count | 54675
| | |
|
GSM319518 | GPL570 |
|
CLL patient ZAP70 low, biological rep7
|
CD19+ purified cells from CLL patient ZAP70 low, biological rep7
|
Sex:M
Age:69
Binet Stage:A
Mutational status: M
ZAP70 -
LPL -
CD38 -
|
Gene expression data from CLL patient expressing low level of ZAP70 measured by real time PCR
|
| Sample_geo_accession | GSM319518
| | Sample_status | Public on Apr 18 2009
| | Sample_submission_date | Sep 10 2008
| | Sample_last_update_date | Apr 18 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Peripheral Blood Mononuclear Cells (PBMC) were obtained from blood of Chronic lymphocytic leukemia patients.
| | Sample_extract_protocol_ch1 | PBMC were isolated by density-gradient centrifugation and B cells were purified with a CD19+ magnetic-bead system.
| | Sample_extract_protocol_ch1 | Total RNA was extracted from purified CD19+ cells in a single step using TriPure Isolation Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1.5 µg total RNA (www.affymetrix.com)
| | Sample_hyb_protocol | Following fragmentation, 16 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix gENESHIP Fluidics Station 450 regarding Affymetrix protocol (www.affymetrix.com)
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Geneship Scanner 3000 7G
| | Sample_data_processing | Image analysis and probe quantification were done with Affymetrix software, which produced raw probe intensity data in the Affymetrix CEL files. Normalization was done with the RMA method, which processed a group of CEL files simultaneously using BRB arrays tools.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Basile,,Stamatopoulos
| | Sample_contact_department | Experimental Hematology
| | Sample_contact_institute | Jules Bordet Institute
| | Sample_contact_address | Bd de Waterloo n°121
| | Sample_contact_city | Bruxelles
| | Sample_contact_zip/postal_code | 1000
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319518/suppl/GSM319518.CEL.gz
| | Sample_series_id | GSE12734
| | Sample_data_row_count | 54675
| | |
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