Search results for the GEO ID: GSE12752  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM319963 | GPL1355 |
|
1st-vehicle
|
1st experiment: neonatal rat cardiomyocytes primary culture cells treated with ethanol for 3 h
|
one-day-old neonatal Wistar rats
|
Gene expression data from 1st experiment of ethanol-treated neonatal rat cardiomyocyte primary culture cells
|
| Sample_geo_accession | GSM319963
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Dec 05 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Primary cultures of cardiomyocytes, grown in OPTI-MEM for 24 h, were treated with vehicle (ethanol) or various ligands for 3 h with or without pretreatment of 10 µM RU486.
| | Sample_growth_protocol_ch1 | The ventricles of 1-day-old neonatal Wistar rats (CLEA Japan, Tokyo, Japan) were dissociated in 0.03% trypsin, 0.03% collagenase, and 20 µg/mL of DNase I. The cardiomyocytes and cardiac fibroblasts were separately prepared on the basis of their differential adhesiveness. Attached cells (mostly cardiac fibroblasts) were subcultured twice to deplete cardiomyocytes, and the third passage cells were used. Cardiomyocytes were seeded at a density of 1x105 cells/cm2 on gelatin-coated dishes and grown in medium 199/DMEM (Invitrogen) supplemented with 10% FCS and antibiotics in a humidified atmosphere at 37˚C with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIZOL-Reagent (Invitrogen) according to the manufacturer’s protocol and further purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3007G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Per-chip normalization was carried out with GeneSpringｨ7.3.1 (Agilent Technologies, Palo Alto, CA). A raw intensity value was divided by the median value of the chip measurements.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | NORITADA,,YOSHIKAWA
| | Sample_contact_email | n-yoshi@ims.u-tokyo.ac.jp
| | Sample_contact_phone | +81-3-5449-5548
| | Sample_contact_fax | +81-3-6409-2098
| | Sample_contact_laboratory | Division of Clinical Immunology
| | Sample_contact_department | Advanced Clinical Research Center
| | Sample_contact_institute | Institute of Medical Science, University of Tokyo
| | Sample_contact_address | Shirokanedai 4-6-1
| | Sample_contact_city | Minatoku, Tokyo
| | Sample_contact_zip/postal_code | 108-8639
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319963/suppl/GSM319963.CEL.gz
| | Sample_series_id | GSE12752
| | Sample_data_row_count | 31099
| | |
|
GSM319964 | GPL1355 |
|
1st-CVZ
|
1st experiment: neonatal rat cardiomyocytes primary culture cells treated with CVZ for 3 h
|
one-day-old neonatal Wistar rats
|
Gene expression data from 1st experiment of CVZ-treated neonatal rat cardiomyocyte primary culture cells
|
| Sample_geo_accession | GSM319964
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Dec 05 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Primary cultures of cardiomyocytes, grown in OPTI-MEM for 24 h, were treated with vehicle (ethanol) or various ligands for 3 h with or without pretreatment of 10 µM RU486.
| | Sample_growth_protocol_ch1 | The ventricles of 1-day-old neonatal Wistar rats (CLEA Japan, Tokyo, Japan) were dissociated in 0.03% trypsin, 0.03% collagenase, and 20 µg/mL of DNase I. The cardiomyocytes and cardiac fibroblasts were separately prepared on the basis of their differential adhesiveness. Attached cells (mostly cardiac fibroblasts) were subcultured twice to deplete cardiomyocytes, and the third passage cells were used. Cardiomyocytes were seeded at a density of 1x105 cells/cm2 on gelatin-coated dishes and grown in medium 199/DMEM (Invitrogen) supplemented with 10% FCS and antibiotics in a humidified atmosphere at 37˚C with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIZOL-Reagent (Invitrogen) according to the manufacturer’s protocol and further purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3007G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Per-chip normalization was carried out with GeneSpringｨ7.3.1 (Agilent Technologies, Palo Alto, CA). A raw intensity value was divided by the median value of the chip measurements.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | NORITADA,,YOSHIKAWA
| | Sample_contact_email | n-yoshi@ims.u-tokyo.ac.jp
| | Sample_contact_phone | +81-3-5449-5548
| | Sample_contact_fax | +81-3-6409-2098
| | Sample_contact_laboratory | Division of Clinical Immunology
| | Sample_contact_department | Advanced Clinical Research Center
| | Sample_contact_institute | Institute of Medical Science, University of Tokyo
| | Sample_contact_address | Shirokanedai 4-6-1
| | Sample_contact_city | Minatoku, Tokyo
| | Sample_contact_zip/postal_code | 108-8639
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319964/suppl/GSM319964.CEL.gz
| | Sample_series_id | GSE12752
| | Sample_data_row_count | 31099
| | |
|
GSM319965 | GPL1355 |
|
1st-COR
|
1st experiment: neonatal rat cardiomyocytes primary culture cells treated with COR for 3 h
|
one-day-old neonatal Wistar rats
|
Gene expression data from 1st experiment of COR-treated neonatal rat cardiomyocyte primary culture cells
|
| Sample_geo_accession | GSM319965
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Dec 05 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Primary cultures of cardiomyocytes, grown in OPTI-MEM for 24 h, were treated with vehicle (ethanol) or various ligands for 3 h with or without pretreatment of 10 µM RU486.
| | Sample_growth_protocol_ch1 | The ventricles of 1-day-old neonatal Wistar rats (CLEA Japan, Tokyo, Japan) were dissociated in 0.03% trypsin, 0.03% collagenase, and 20 µg/mL of DNase I. The cardiomyocytes and cardiac fibroblasts were separately prepared on the basis of their differential adhesiveness. Attached cells (mostly cardiac fibroblasts) were subcultured twice to deplete cardiomyocytes, and the third passage cells were used. Cardiomyocytes were seeded at a density of 1x105 cells/cm2 on gelatin-coated dishes and grown in medium 199/DMEM (Invitrogen) supplemented with 10% FCS and antibiotics in a humidified atmosphere at 37˚C with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIZOL-Reagent (Invitrogen) according to the manufacturer’s protocol and further purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3007G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Per-chip normalization was carried out with GeneSpringｨ7.3.1 (Agilent Technologies, Palo Alto, CA). A raw intensity value was divided by the median value of the chip measurements.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | NORITADA,,YOSHIKAWA
| | Sample_contact_email | n-yoshi@ims.u-tokyo.ac.jp
| | Sample_contact_phone | +81-3-5449-5548
| | Sample_contact_fax | +81-3-6409-2098
| | Sample_contact_laboratory | Division of Clinical Immunology
| | Sample_contact_department | Advanced Clinical Research Center
| | Sample_contact_institute | Institute of Medical Science, University of Tokyo
| | Sample_contact_address | Shirokanedai 4-6-1
| | Sample_contact_city | Minatoku, Tokyo
| | Sample_contact_zip/postal_code | 108-8639
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319965/suppl/GSM319965.CEL.gz
| | Sample_series_id | GSE12752
| | Sample_data_row_count | 31099
| | |
|
GSM319966 | GPL1355 |
|
1st-ALD
|
1st experiment: neonatal rat cardiomyocytes primary culture cells treated with ALD for 3 h
|
one-day-old neonatal Wistar rats
|
Gene expression data from 1st experiment of ALD-treated neonatal rat cardiomyocyte primary culture cells
|
| Sample_geo_accession | GSM319966
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Dec 05 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Primary cultures of cardiomyocytes, grown in OPTI-MEM for 24 h, were treated with vehicle (ethanol) or various ligands for 3 h with or without pretreatment of 10 µM RU486.
| | Sample_growth_protocol_ch1 | The ventricles of 1-day-old neonatal Wistar rats (CLEA Japan, Tokyo, Japan) were dissociated in 0.03% trypsin, 0.03% collagenase, and 20 µg/mL of DNase I. The cardiomyocytes and cardiac fibroblasts were separately prepared on the basis of their differential adhesiveness. Attached cells (mostly cardiac fibroblasts) were subcultured twice to deplete cardiomyocytes, and the third passage cells were used. Cardiomyocytes were seeded at a density of 1x105 cells/cm2 on gelatin-coated dishes and grown in medium 199/DMEM (Invitrogen) supplemented with 10% FCS and antibiotics in a humidified atmosphere at 37˚C with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIZOL-Reagent (Invitrogen) according to the manufacturer’s protocol and further purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3007G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Per-chip normalization was carried out with GeneSpringｨ7.3.1 (Agilent Technologies, Palo Alto, CA). A raw intensity value was divided by the median value of the chip measurements.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | NORITADA,,YOSHIKAWA
| | Sample_contact_email | n-yoshi@ims.u-tokyo.ac.jp
| | Sample_contact_phone | +81-3-5449-5548
| | Sample_contact_fax | +81-3-6409-2098
| | Sample_contact_laboratory | Division of Clinical Immunology
| | Sample_contact_department | Advanced Clinical Research Center
| | Sample_contact_institute | Institute of Medical Science, University of Tokyo
| | Sample_contact_address | Shirokanedai 4-6-1
| | Sample_contact_city | Minatoku, Tokyo
| | Sample_contact_zip/postal_code | 108-8639
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319966/suppl/GSM319966.CEL.gz
| | Sample_series_id | GSE12752
| | Sample_data_row_count | 31099
| | |
|
GSM319967 | GPL1355 |
|
2nd-vehicle
|
2nd experiment; neonatal rat cardiomyocytes primary culture cells treated with ethanol for 3 h
|
one-day-old neonatal Wistar rats
|
Gene expression data from 2nd experiment of ethanol-treated neonatal rat cardiomyocyte primary culture cells
|
| Sample_geo_accession | GSM319967
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Dec 05 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Primary cultures of cardiomyocytes, grown in OPTI-MEM for 24 h, were treated with vehicle (ethanol) or various ligands for 3 h with or without pretreatment of 10 µM RU486.
| | Sample_growth_protocol_ch1 | The ventricles of 1-day-old neonatal Wistar rats (CLEA Japan, Tokyo, Japan) were dissociated in 0.03% trypsin, 0.03% collagenase, and 20 µg/mL of DNase I. The cardiomyocytes and cardiac fibroblasts were separately prepared on the basis of their differential adhesiveness. Attached cells (mostly cardiac fibroblasts) were subcultured twice to deplete cardiomyocytes, and the third passage cells were used. Cardiomyocytes were seeded at a density of 1x105 cells/cm2 on gelatin-coated dishes and grown in medium 199/DMEM (Invitrogen) supplemented with 10% FCS and antibiotics in a humidified atmosphere at 37˚C with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIZOL-Reagent (Invitrogen) according to the manufacturer’s protocol and further purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3007G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Per-chip normalization was carried out with GeneSpringｨ7.3.1 (Agilent Technologies, Palo Alto, CA). A raw intensity value was divided by the median value of the chip measurements.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | NORITADA,,YOSHIKAWA
| | Sample_contact_email | n-yoshi@ims.u-tokyo.ac.jp
| | Sample_contact_phone | +81-3-5449-5548
| | Sample_contact_fax | +81-3-6409-2098
| | Sample_contact_laboratory | Division of Clinical Immunology
| | Sample_contact_department | Advanced Clinical Research Center
| | Sample_contact_institute | Institute of Medical Science, University of Tokyo
| | Sample_contact_address | Shirokanedai 4-6-1
| | Sample_contact_city | Minatoku, Tokyo
| | Sample_contact_zip/postal_code | 108-8639
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319967/suppl/GSM319967.CEL.gz
| | Sample_series_id | GSE12752
| | Sample_data_row_count | 31099
| | |
|
GSM319968 | GPL1355 |
|
2nd-CVZ
|
2nd experiment; neonatal rat cardiomyocytes primary culture cells treated with CVZ for 3 h
|
one-day-old neonatal Wistar rats
|
Gene expression data from 2nd experiment of CVZ-treated neonatal rat cardiomyocyte primary culture cells
|
| Sample_geo_accession | GSM319968
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Dec 05 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Primary cultures of cardiomyocytes, grown in OPTI-MEM for 24 h, were treated with vehicle (ethanol) or various ligands for 3 h with or without pretreatment of 10 µM RU486.
| | Sample_growth_protocol_ch1 | The ventricles of 1-day-old neonatal Wistar rats (CLEA Japan, Tokyo, Japan) were dissociated in 0.03% trypsin, 0.03% collagenase, and 20 µg/mL of DNase I. The cardiomyocytes and cardiac fibroblasts were separately prepared on the basis of their differential adhesiveness. Attached cells (mostly cardiac fibroblasts) were subcultured twice to deplete cardiomyocytes, and the third passage cells were used. Cardiomyocytes were seeded at a density of 1x105 cells/cm2 on gelatin-coated dishes and grown in medium 199/DMEM (Invitrogen) supplemented with 10% FCS and antibiotics in a humidified atmosphere at 37˚C with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIZOL-Reagent (Invitrogen) according to the manufacturer’s protocol and further purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3007G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Per-chip normalization was carried out with GeneSpringｨ7.3.1 (Agilent Technologies, Palo Alto, CA). A raw intensity value was divided by the median value of the chip measurements.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | NORITADA,,YOSHIKAWA
| | Sample_contact_email | n-yoshi@ims.u-tokyo.ac.jp
| | Sample_contact_phone | +81-3-5449-5548
| | Sample_contact_fax | +81-3-6409-2098
| | Sample_contact_laboratory | Division of Clinical Immunology
| | Sample_contact_department | Advanced Clinical Research Center
| | Sample_contact_institute | Institute of Medical Science, University of Tokyo
| | Sample_contact_address | Shirokanedai 4-6-1
| | Sample_contact_city | Minatoku, Tokyo
| | Sample_contact_zip/postal_code | 108-8639
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319968/suppl/GSM319968.CEL.gz
| | Sample_series_id | GSE12752
| | Sample_data_row_count | 31099
| | |
|
GSM319969 | GPL1355 |
|
2nd-COR
|
2nd experiment; neonatal rat cardiomyocytes primary culture cells treated with COR for 3 h
|
one-day-old neonatal Wistar rats
|
Gene expression data from 2nd experiment of COR-treated neonatal rat cardiomyocyte primary culture cells
|
| Sample_geo_accession | GSM319969
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Dec 05 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Primary cultures of cardiomyocytes, grown in OPTI-MEM for 24 h, were treated with vehicle (ethanol) or various ligands for 3 h with or without pretreatment of 10 µM RU486.
| | Sample_growth_protocol_ch1 | The ventricles of 1-day-old neonatal Wistar rats (CLEA Japan, Tokyo, Japan) were dissociated in 0.03% trypsin, 0.03% collagenase, and 20 µg/mL of DNase I. The cardiomyocytes and cardiac fibroblasts were separately prepared on the basis of their differential adhesiveness. Attached cells (mostly cardiac fibroblasts) were subcultured twice to deplete cardiomyocytes, and the third passage cells were used. Cardiomyocytes were seeded at a density of 1x105 cells/cm2 on gelatin-coated dishes and grown in medium 199/DMEM (Invitrogen) supplemented with 10% FCS and antibiotics in a humidified atmosphere at 37˚C with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIZOL-Reagent (Invitrogen) according to the manufacturer’s protocol and further purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3007G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Per-chip normalization was carried out with GeneSpringｨ7.3.1 (Agilent Technologies, Palo Alto, CA). A raw intensity value was divided by the median value of the chip measurements.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | NORITADA,,YOSHIKAWA
| | Sample_contact_email | n-yoshi@ims.u-tokyo.ac.jp
| | Sample_contact_phone | +81-3-5449-5548
| | Sample_contact_fax | +81-3-6409-2098
| | Sample_contact_laboratory | Division of Clinical Immunology
| | Sample_contact_department | Advanced Clinical Research Center
| | Sample_contact_institute | Institute of Medical Science, University of Tokyo
| | Sample_contact_address | Shirokanedai 4-6-1
| | Sample_contact_city | Minatoku, Tokyo
| | Sample_contact_zip/postal_code | 108-8639
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319969/suppl/GSM319969.CEL.gz
| | Sample_series_id | GSE12752
| | Sample_data_row_count | 31099
| | |
|
GSM319970 | GPL1355 |
|
2nd-ALD
|
2nd experiment; neonatal rat cardiomyocytes primary culture cells treated with ALD for 3 h
|
one-day-old neonatal Wistar rats
|
Gene expression data from 2nd experiment of ALD-treated neonatal rat cardiomyocyte primary culture cells
|
| Sample_geo_accession | GSM319970
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Dec 05 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Primary cultures of cardiomyocytes, grown in OPTI-MEM for 24 h, were treated with vehicle (ethanol) or various ligands for 3 h with or without pretreatment of 10 µM RU486.
| | Sample_growth_protocol_ch1 | The ventricles of 1-day-old neonatal Wistar rats (CLEA Japan, Tokyo, Japan) were dissociated in 0.03% trypsin, 0.03% collagenase, and 20 µg/mL of DNase I. The cardiomyocytes and cardiac fibroblasts were separately prepared on the basis of their differential adhesiveness. Attached cells (mostly cardiac fibroblasts) were subcultured twice to deplete cardiomyocytes, and the third passage cells were used. Cardiomyocytes were seeded at a density of 1x105 cells/cm2 on gelatin-coated dishes and grown in medium 199/DMEM (Invitrogen) supplemented with 10% FCS and antibiotics in a humidified atmosphere at 37˚C with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIZOL-Reagent (Invitrogen) according to the manufacturer’s protocol and further purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3007G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Per-chip normalization was carried out with GeneSpringｨ7.3.1 (Agilent Technologies, Palo Alto, CA). A raw intensity value was divided by the median value of the chip measurements.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | NORITADA,,YOSHIKAWA
| | Sample_contact_email | n-yoshi@ims.u-tokyo.ac.jp
| | Sample_contact_phone | +81-3-5449-5548
| | Sample_contact_fax | +81-3-6409-2098
| | Sample_contact_laboratory | Division of Clinical Immunology
| | Sample_contact_department | Advanced Clinical Research Center
| | Sample_contact_institute | Institute of Medical Science, University of Tokyo
| | Sample_contact_address | Shirokanedai 4-6-1
| | Sample_contact_city | Minatoku, Tokyo
| | Sample_contact_zip/postal_code | 108-8639
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319970/suppl/GSM319970.CEL.gz
| | Sample_series_id | GSE12752
| | Sample_data_row_count | 31099
| | |
|
GSM319971 | GPL1355 |
|
2nd-RU
|
2nd experiment; neonatal rat cardiomyocytes primary culture cells treated with RU486 for 3 h
|
one-day-old neonatal Wistar rats
|
Gene expression data from 2nd experiment of RU486-treated neonatal rat cardiomyocyte primary culture cells
|
| Sample_geo_accession | GSM319971
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Dec 05 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Primary cultures of cardiomyocytes, grown in OPTI-MEM for 24 h, were treated with vehicle (ethanol) or various ligands for 3 h with or without pretreatment of 10 µM RU486.
| | Sample_growth_protocol_ch1 | The ventricles of 1-day-old neonatal Wistar rats (CLEA Japan, Tokyo, Japan) were dissociated in 0.03% trypsin, 0.03% collagenase, and 20 µg/mL of DNase I. The cardiomyocytes and cardiac fibroblasts were separately prepared on the basis of their differential adhesiveness. Attached cells (mostly cardiac fibroblasts) were subcultured twice to deplete cardiomyocytes, and the third passage cells were used. Cardiomyocytes were seeded at a density of 1x105 cells/cm2 on gelatin-coated dishes and grown in medium 199/DMEM (Invitrogen) supplemented with 10% FCS and antibiotics in a humidified atmosphere at 37˚C with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIZOL-Reagent (Invitrogen) according to the manufacturer’s protocol and further purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3007G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Per-chip normalization was carried out with GeneSpringｨ7.3.1 (Agilent Technologies, Palo Alto, CA). A raw intensity value was divided by the median value of the chip measurements.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | NORITADA,,YOSHIKAWA
| | Sample_contact_email | n-yoshi@ims.u-tokyo.ac.jp
| | Sample_contact_phone | +81-3-5449-5548
| | Sample_contact_fax | +81-3-6409-2098
| | Sample_contact_laboratory | Division of Clinical Immunology
| | Sample_contact_department | Advanced Clinical Research Center
| | Sample_contact_institute | Institute of Medical Science, University of Tokyo
| | Sample_contact_address | Shirokanedai 4-6-1
| | Sample_contact_city | Minatoku, Tokyo
| | Sample_contact_zip/postal_code | 108-8639
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319971/suppl/GSM319971.CEL.gz
| | Sample_series_id | GSE12752
| | Sample_data_row_count | 31099
| | |
|
GSM319972 | GPL1355 |
|
2nd-CVZ+RU
|
2nd experiment; neonatal rat cardiomyocytes primary culture cells treated with CVZ and RU486 for 3 h
|
one-day-old neonatal Wistar rats
|
Gene expression data from 2nd experiment of CVZ and RU486-treated neonatal rat cardiomyocyte primary culture cells
|
| Sample_geo_accession | GSM319972
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Dec 05 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Primary cultures of cardiomyocytes, grown in OPTI-MEM for 24 h, were treated with vehicle (ethanol) or various ligands for 3 h with or without pretreatment of 10 µM RU486.
| | Sample_growth_protocol_ch1 | The ventricles of 1-day-old neonatal Wistar rats (CLEA Japan, Tokyo, Japan) were dissociated in 0.03% trypsin, 0.03% collagenase, and 20 µg/mL of DNase I. The cardiomyocytes and cardiac fibroblasts were separately prepared on the basis of their differential adhesiveness. Attached cells (mostly cardiac fibroblasts) were subcultured twice to deplete cardiomyocytes, and the third passage cells were used. Cardiomyocytes were seeded at a density of 1x105 cells/cm2 on gelatin-coated dishes and grown in medium 199/DMEM (Invitrogen) supplemented with 10% FCS and antibiotics in a humidified atmosphere at 37˚C with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIZOL-Reagent (Invitrogen) according to the manufacturer’s protocol and further purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3007G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Per-chip normalization was carried out with GeneSpringｨ7.3.1 (Agilent Technologies, Palo Alto, CA). A raw intensity value was divided by the median value of the chip measurements.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | NORITADA,,YOSHIKAWA
| | Sample_contact_email | n-yoshi@ims.u-tokyo.ac.jp
| | Sample_contact_phone | +81-3-5449-5548
| | Sample_contact_fax | +81-3-6409-2098
| | Sample_contact_laboratory | Division of Clinical Immunology
| | Sample_contact_department | Advanced Clinical Research Center
| | Sample_contact_institute | Institute of Medical Science, University of Tokyo
| | Sample_contact_address | Shirokanedai 4-6-1
| | Sample_contact_city | Minatoku, Tokyo
| | Sample_contact_zip/postal_code | 108-8639
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319972/suppl/GSM319972.CEL.gz
| | Sample_series_id | GSE12752
| | Sample_data_row_count | 31099
| | |
|
GSM319973 | GPL1355 |
|
2nd-COR+RU
|
2nd experiment; neonatal rat cardiomyocytes primary culture cells treated with COR and RU486 for 3 h
|
one-day-old neonatal Wistar rats
|
Gene expression data from 2nd experiment of COR and RU486-treated neonatal rat cardiomyocyte primary culture cells
|
| Sample_geo_accession | GSM319973
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Dec 05 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Primary cultures of cardiomyocytes, grown in OPTI-MEM for 24 h, were treated with vehicle (ethanol) or various ligands for 3 h with or without pretreatment of 10 µM RU486.
| | Sample_growth_protocol_ch1 | The ventricles of 1-day-old neonatal Wistar rats (CLEA Japan, Tokyo, Japan) were dissociated in 0.03% trypsin, 0.03% collagenase, and 20 µg/mL of DNase I. The cardiomyocytes and cardiac fibroblasts were separately prepared on the basis of their differential adhesiveness. Attached cells (mostly cardiac fibroblasts) were subcultured twice to deplete cardiomyocytes, and the third passage cells were used. Cardiomyocytes were seeded at a density of 1x105 cells/cm2 on gelatin-coated dishes and grown in medium 199/DMEM (Invitrogen) supplemented with 10% FCS and antibiotics in a humidified atmosphere at 37˚C with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIZOL-Reagent (Invitrogen) according to the manufacturer’s protocol and further purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3007G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Per-chip normalization was carried out with GeneSpringｨ7.3.1 (Agilent Technologies, Palo Alto, CA). A raw intensity value was divided by the median value of the chip measurements.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | NORITADA,,YOSHIKAWA
| | Sample_contact_email | n-yoshi@ims.u-tokyo.ac.jp
| | Sample_contact_phone | +81-3-5449-5548
| | Sample_contact_fax | +81-3-6409-2098
| | Sample_contact_laboratory | Division of Clinical Immunology
| | Sample_contact_department | Advanced Clinical Research Center
| | Sample_contact_institute | Institute of Medical Science, University of Tokyo
| | Sample_contact_address | Shirokanedai 4-6-1
| | Sample_contact_city | Minatoku, Tokyo
| | Sample_contact_zip/postal_code | 108-8639
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319973/suppl/GSM319973.CEL.gz
| | Sample_series_id | GSE12752
| | Sample_data_row_count | 31099
| | |
|
GSM319974 | GPL1355 |
|
2nd-ALD+RU
|
2nd experiment; neonatal rat cardiomyocytes primary culture cells treated with ALD and RU486 for 3 h
|
one-day-old neonatal Wistar rats
|
Gene expression data from 2nd experiment of ALD and RU486-treated neonatal rat cardiomyocyte primary culture cells
|
| Sample_geo_accession | GSM319974
| | Sample_status | Public on May 01 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Dec 05 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Primary cultures of cardiomyocytes, grown in OPTI-MEM for 24 h, were treated with vehicle (ethanol) or various ligands for 3 h with or without pretreatment of 10 µM RU486.
| | Sample_growth_protocol_ch1 | The ventricles of 1-day-old neonatal Wistar rats (CLEA Japan, Tokyo, Japan) were dissociated in 0.03% trypsin, 0.03% collagenase, and 20 µg/mL of DNase I. The cardiomyocytes and cardiac fibroblasts were separately prepared on the basis of their differential adhesiveness. Attached cells (mostly cardiac fibroblasts) were subcultured twice to deplete cardiomyocytes, and the third passage cells were used. Cardiomyocytes were seeded at a density of 1x105 cells/cm2 on gelatin-coated dishes and grown in medium 199/DMEM (Invitrogen) supplemented with 10% FCS and antibiotics in a humidified atmosphere at 37˚C with 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIZOL-Reagent (Invitrogen) according to the manufacturer’s protocol and further purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3007G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings. Per-chip normalization was carried out with GeneSpringｨ7.3.1 (Agilent Technologies, Palo Alto, CA). A raw intensity value was divided by the median value of the chip measurements.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | NORITADA,,YOSHIKAWA
| | Sample_contact_email | n-yoshi@ims.u-tokyo.ac.jp
| | Sample_contact_phone | +81-3-5449-5548
| | Sample_contact_fax | +81-3-6409-2098
| | Sample_contact_laboratory | Division of Clinical Immunology
| | Sample_contact_department | Advanced Clinical Research Center
| | Sample_contact_institute | Institute of Medical Science, University of Tokyo
| | Sample_contact_address | Shirokanedai 4-6-1
| | Sample_contact_city | Minatoku, Tokyo
| | Sample_contact_zip/postal_code | 108-8639
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319974/suppl/GSM319974.CEL.gz
| | Sample_series_id | GSE12752
| | Sample_data_row_count | 31099
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
