Search results for the GEO ID: GSE12758 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM319987 | GPL1355 |
|
Left atria shunt rep1 (set 1)
|
Left atrial tissue following 28 days aorto-caval shunt operation
|
strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM319987
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319987/suppl/GSM319987.CEL.gz
| Sample_series_id | GSE12754
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM319988 | GPL1355 |
|
Left atria shunt rep2 (set 1)
|
Left atrial tissue following 28 days aorto-caval shunt operation
|
strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM319988
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319988/suppl/GSM319988.CEL.gz
| Sample_series_id | GSE12754
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM319989 | GPL1355 |
|
Left atria shunt rep3 (set 1)
|
Left atrial tissue following 28 days aorto-caval shunt operation
|
strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM319989
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319989/suppl/GSM319989.CEL.gz
| Sample_series_id | GSE12754
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM319990 | GPL1355 |
|
Left atria sham rep1 (set 1)
|
Left atrial tissue following 28 days aorto-caval sham operation
|
strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM319990
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319990/suppl/GSM319990.CEL.gz
| Sample_series_id | GSE12754
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM319991 | GPL1355 |
|
Left atria sham rep2 (set 1)
|
Left atrial tissue following 28 days aorto-caval sham operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM319991
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319991/suppl/GSM319991.CEL.gz
| Sample_series_id | GSE12754
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM319992 | GPL1355 |
|
Left atria sham rep3 (set 1)
|
Left atrial tissue following 28 days aorto-caval sham operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM319992
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319992/suppl/GSM319992.CEL.gz
| Sample_series_id | GSE12754
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM319993 | GPL1355 |
|
Left ventricles shunt rep1 (set 1)
|
Left ventricular tissue following 28 days aorto-caval shunt operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM319993
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319993/suppl/GSM319993.CEL.gz
| Sample_series_id | GSE12754
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM319994 | GPL1355 |
|
Left ventricles shunt rep2 (set 1)
|
Left ventricular tissue following 28 days aorto-caval shunt operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM319994
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319994/suppl/GSM319994.CEL.gz
| Sample_series_id | GSE12754
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM319995 | GPL1355 |
|
Left ventricles shunt rep3 (set 1)
|
Left ventricular tissue following 28 days aorto-caval shunt operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM319995
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319995/suppl/GSM319995.CEL.gz
| Sample_series_id | GSE12754
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM319996 | GPL1355 |
|
Left ventricles sham rep1 (set 1)
|
Left ventricular tissue following 28 days aorto-caval sham operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM319996
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319996/suppl/GSM319996.CEL.gz
| Sample_series_id | GSE12754
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM319997 | GPL1355 |
|
Left ventricles sham rep2 (set 1)
|
Left ventricular tissue following 28 days aorto-caval sham operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM319997
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319997/suppl/GSM319997.CEL.gz
| Sample_series_id | GSE12754
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM319998 | GPL1355 |
|
Left ventricles sham rep3 (set 1)
|
Left ventricular tissue following 28 days aorto-caval sham operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM319998
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319998/suppl/GSM319998.CEL.gz
| Sample_series_id | GSE12754
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM319999 | GPL1355 |
|
Right atria shunt rep1 (set 2)
|
Right atrial tissue following 28 days aorto-caval shunt operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM319999
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM319nnn/GSM319999/suppl/GSM319999.CEL.gz
| Sample_series_id | GSE12755
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM320000 | GPL1355 |
|
Right atria shunt rep2 (set 2)
|
Right atrial tissue following 28 days aorto-caval shunt operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM320000
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320000/suppl/GSM320000.CEL.gz
| Sample_series_id | GSE12755
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM320001 | GPL1355 |
|
Right atria shunt rep3 (set 2)
|
Right atrial tissue following 28 days aorto-caval shunt operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM320001
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320001/suppl/GSM320001.CEL.gz
| Sample_series_id | GSE12755
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM320002 | GPL1355 |
|
Right atria sham rep1 (set 2)
|
Right atrial tissue following 28 days aorto-caval sham operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM320002
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320002/suppl/GSM320002.CEL.gz
| Sample_series_id | GSE12755
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM320003 | GPL1355 |
|
Right atria sham rep2 (set 2)
|
Right atrial tissue following 28 days aorto-caval sham operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM320003
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320003/suppl/GSM320003.CEL.gz
| Sample_series_id | GSE12755
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM320004 | GPL1355 |
|
Right atria sham rep3 (set 2)
|
Right atrial tissue following 28 days aorto-caval sham operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM320004
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320004/suppl/GSM320004.CEL.gz
| Sample_series_id | GSE12755
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM320005 | GPL1355 |
|
Left atria shunt rep1 (set 2)
|
Left atrial tissue following 28 days aorto-caval shunt operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM320005
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320005/suppl/GSM320005.CEL.gz
| Sample_series_id | GSE12755
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM320006 | GPL1355 |
|
Left atria shunt rep2 (set 2)
|
Left atrial tissue following 28 days aorto-caval shunt operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM320006
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320006/suppl/GSM320006.CEL.gz
| Sample_series_id | GSE12755
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM320007 | GPL1355 |
|
Left atria shunt rep3 (set 2)
|
Left atrial tissue following 28 days aorto-caval shunt operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM320007
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320007/suppl/GSM320007.CEL.gz
| Sample_series_id | GSE12755
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM320008 | GPL1355 |
|
Left atria sham rep1 (set 2)
|
Left atrial tissue following 28 days aorto-caval sham operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM320008
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320008/suppl/GSM320008.CEL.gz
| Sample_series_id | GSE12755
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM320009 | GPL1355 |
|
Left atria sham rep2 (set 2)
|
Left atrial tissue following 28 days aorto-caval sham operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM320009
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320009/suppl/GSM320009.CEL.gz
| Sample_series_id | GSE12755
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
GSM320010 | GPL1355 |
|
Left atria sham rep3 (set 2)
|
Left atrial tissue following 28 days aorto-caval sham operation
|
Strain: Sprague-Dawley; Gender: male; Weight: 225-275 g; Tissue: Heart atria and ventricles
|
n/a
|
Sample_geo_accession | GSM320010
| Sample_status | Public on Aug 01 2010
| Sample_submission_date | Sep 12 2008
| Sample_last_update_date | Aug 24 2009
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Tissue was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen mini columns according to the manufacturer's instructions.
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | A total of 20 ug of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
| Sample_data_processing | The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
| Sample_platform_id | GPL1355
| Sample_contact_name | Adolfo,J,de Bold
| Sample_contact_email | adebold@ottawaheart.ca
| Sample_contact_phone | (613) 761-4265
| Sample_contact_fax | (613) 761-1597
| Sample_contact_department | Cardiovascular Endocrinology
| Sample_contact_institute | University of Ottawa Heart Institute
| Sample_contact_address | 40 Ruskin St.
| Sample_contact_city | Ottawa
| Sample_contact_state | ON
| Sample_contact_zip/postal_code | K1Y 4W7
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320010/suppl/GSM320010.CEL.gz
| Sample_series_id | GSE12755
| Sample_series_id | GSE12758
| Sample_data_row_count | 31099
| |
|
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