Search results for the GEO ID: GSE12764  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM320243 | GPL570 |
|
MCF10a_Null_Vector_rep1
|
MCF10a treated with null vector 24h
|
MCF10a immortalized, non-transformed human mammary epithelial cell-line overexpressing null vector
|
MCF10a_Null_Vector_rep1
|
| Sample_geo_accession | GSM320243
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Sep 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were optimally transfected with null, HRAS or MEK1 vectors. Cells lysed 24h post-transfection
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM/F12 with 15 mM hepes buffer supplemented with 5% horse serum, 10 ug/ml insulin, 20 ng/ml EGF, 100 ng/ml choleratoxin, 0.5 ug/ml hydrocortisone pen/strep
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy kit used for the extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133P 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mark,,Lackner
| | Sample_contact_email | lackner.mark@gene.com
| | Sample_contact_institute | Genentech
| | Sample_contact_address | 1 DNA Way
| | Sample_contact_city | South San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94080
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320243/suppl/GSM320243.CEL.gz
| | Sample_series_id | GSE12764
| | Sample_series_id | GSE12790
| | Sample_data_row_count | 54675
| | |
|
GSM320244 | GPL570 |
|
MCF10a_Null_Vector_rep2
|
MCF10a treated with null vector 24h
|
MCF10a immortalized, non-transformed human mammary epithelial cell-line overexpressing null vector
|
MCF10a_Null_Vector_rep2
|
| Sample_geo_accession | GSM320244
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Sep 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were optimally transfected with null, HRAS or MEK1 vectors. Cells lysed 24h post-transfection
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM/F12 with 15 mM hepes buffer supplemented with 5% horse serum, 10 ug/ml insulin, 20 ng/ml EGF, 100 ng/ml choleratoxin, 0.5 ug/ml hydrocortisone pen/strep
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy kit used for the extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133P 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mark,,Lackner
| | Sample_contact_email | lackner.mark@gene.com
| | Sample_contact_institute | Genentech
| | Sample_contact_address | 1 DNA Way
| | Sample_contact_city | South San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94080
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320244/suppl/GSM320244.CEL.gz
| | Sample_series_id | GSE12764
| | Sample_series_id | GSE12790
| | Sample_data_row_count | 54675
| | |
|
GSM320245 | GPL570 |
|
MCF10a_Null_Vector_rep3
|
MCF10a treated with null vector 24h
|
MCF10a immortalized, non-transformed human mammary epithelial cell-line overexpressing null vector
|
MCF10a_Null_Vector_rep3
|
| Sample_geo_accession | GSM320245
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Sep 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were optimally transfected with null, HRAS or MEK1 vectors. Cells lysed 24h post-transfection
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM/F12 with 15 mM hepes buffer supplemented with 5% horse serum, 10 ug/ml insulin, 20 ng/ml EGF, 100 ng/ml choleratoxin, 0.5 ug/ml hydrocortisone pen/strep
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy kit used for the extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133P 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mark,,Lackner
| | Sample_contact_email | lackner.mark@gene.com
| | Sample_contact_institute | Genentech
| | Sample_contact_address | 1 DNA Way
| | Sample_contact_city | South San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94080
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320245/suppl/GSM320245.CEL.gz
| | Sample_series_id | GSE12764
| | Sample_series_id | GSE12790
| | Sample_data_row_count | 54675
| | |
|
GSM320246 | GPL570 |
|
MCF10a_Null_Vector_rep4
|
MCF10a treated with null vector 24h
|
MCF10a immortalized, non-transformed human mammary epithelial cell-line overexpressing null vector
|
MCF10a_Null_Vector_rep4
|
| Sample_geo_accession | GSM320246
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Sep 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were optimally transfected with null, HRAS or MEK1 vectors. Cells lysed 24h post-transfection
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM/F12 with 15 mM hepes buffer supplemented with 5% horse serum, 10 ug/ml insulin, 20 ng/ml EGF, 100 ng/ml choleratoxin, 0.5 ug/ml hydrocortisone pen/strep
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy kit used for the extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133P 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mark,,Lackner
| | Sample_contact_email | lackner.mark@gene.com
| | Sample_contact_institute | Genentech
| | Sample_contact_address | 1 DNA Way
| | Sample_contact_city | South San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94080
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320246/suppl/GSM320246.CEL.gz
| | Sample_series_id | GSE12764
| | Sample_series_id | GSE12790
| | Sample_data_row_count | 54675
| | |
|
GSM320247 | GPL570 |
|
MCF10a_Null_Vector_rep5
|
MCF10a treated with null vector 24h
|
MCF10a immortalized, non-transformed human mammary epithelial cell-line overexpressing null vector
|
MCF10a_Null_Vector_rep5
|
| Sample_geo_accession | GSM320247
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Sep 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were optimally transfected with null, HRAS or MEK1 vectors. Cells lysed 24h post-transfection
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM/F12 with 15 mM hepes buffer supplemented with 5% horse serum, 10 ug/ml insulin, 20 ng/ml EGF, 100 ng/ml choleratoxin, 0.5 ug/ml hydrocortisone pen/strep
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy kit used for the extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133P 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mark,,Lackner
| | Sample_contact_email | lackner.mark@gene.com
| | Sample_contact_institute | Genentech
| | Sample_contact_address | 1 DNA Way
| | Sample_contact_city | South San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94080
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320247/suppl/GSM320247.CEL.gz
| | Sample_series_id | GSE12764
| | Sample_series_id | GSE12790
| | Sample_data_row_count | 54675
| | |
|
GSM320248 | GPL570 |
|
MCF10a_Null_Vector_rep6
|
MCF10a treated with null vector 24h
|
MCF10a immortalized, non-transformed human mammary epithelial cell-line overexpressing null vector
|
MCF10a_Null_Vector_rep6
|
| Sample_geo_accession | GSM320248
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Sep 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were optimally transfected with null, HRAS or MEK1 vectors. Cells lysed 24h post-transfection
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM/F12 with 15 mM hepes buffer supplemented with 5% horse serum, 10 ug/ml insulin, 20 ng/ml EGF, 100 ng/ml choleratoxin, 0.5 ug/ml hydrocortisone pen/strep
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy kit used for the extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133P 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mark,,Lackner
| | Sample_contact_email | lackner.mark@gene.com
| | Sample_contact_institute | Genentech
| | Sample_contact_address | 1 DNA Way
| | Sample_contact_city | South San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94080
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320248/suppl/GSM320248.CEL.gz
| | Sample_series_id | GSE12764
| | Sample_series_id | GSE12790
| | Sample_data_row_count | 54675
| | |
|
GSM320249 | GPL570 |
|
MCF10a_HRas_Vector_rep1
|
MCF10a treated with HRas vector 24h
|
MCF10a immortalized, non-transformed human mammary epithelial cell-line overexpressing RAS
|
MCF10a_HRas_Vector_rep1
|
| Sample_geo_accession | GSM320249
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Sep 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were optimally transfected with null, HRAS or MEK1 vectors. Cells lysed 24h post-transfection
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM/F12 with 15 mM hepes buffer supplemented with 5% horse serum, 10 ug/ml insulin, 20 ng/ml EGF, 100 ng/ml choleratoxin, 0.5 ug/ml hydrocortisone pen/strep
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy kit used for the extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133P 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mark,,Lackner
| | Sample_contact_email | lackner.mark@gene.com
| | Sample_contact_institute | Genentech
| | Sample_contact_address | 1 DNA Way
| | Sample_contact_city | South San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94080
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320249/suppl/GSM320249.CEL.gz
| | Sample_series_id | GSE12764
| | Sample_series_id | GSE12790
| | Sample_data_row_count | 54675
| | |
|
GSM320250 | GPL570 |
|
MCF10a_HRas_Vector_rep2
|
MCF10a treated with HRas vector 24h
|
MCF10a immortalized, non-transformed human mammary epithelial cell-line overexpressing RAS
|
MCF10a_HRas_Vector_rep2
|
| Sample_geo_accession | GSM320250
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Sep 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were optimally transfected with null, HRAS or MEK1 vectors. Cells lysed 24h post-transfection
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM/F12 with 15 mM hepes buffer supplemented with 5% horse serum, 10 ug/ml insulin, 20 ng/ml EGF, 100 ng/ml choleratoxin, 0.5 ug/ml hydrocortisone pen/strep
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy kit used for the extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133P 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mark,,Lackner
| | Sample_contact_email | lackner.mark@gene.com
| | Sample_contact_institute | Genentech
| | Sample_contact_address | 1 DNA Way
| | Sample_contact_city | South San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94080
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320250/suppl/GSM320250.CEL.gz
| | Sample_series_id | GSE12764
| | Sample_series_id | GSE12790
| | Sample_data_row_count | 54675
| | |
|
GSM320251 | GPL570 |
|
MCF10a_HRas_Vector_rep3
|
MCF10a treated with HRas vector 24h
|
MCF10a immortalized, non-transformed human mammary epithelial cell-line overexpressing RAS
|
MCF10a_HRas_Vector_rep3
|
| Sample_geo_accession | GSM320251
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Sep 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were optimally transfected with null, HRAS or MEK1 vectors. Cells lysed 24h post-transfection
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM/F12 with 15 mM hepes buffer supplemented with 5% horse serum, 10 ug/ml insulin, 20 ng/ml EGF, 100 ng/ml choleratoxin, 0.5 ug/ml hydrocortisone pen/strep
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy kit used for the extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133P 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mark,,Lackner
| | Sample_contact_email | lackner.mark@gene.com
| | Sample_contact_institute | Genentech
| | Sample_contact_address | 1 DNA Way
| | Sample_contact_city | South San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94080
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320251/suppl/GSM320251.CEL.gz
| | Sample_series_id | GSE12764
| | Sample_series_id | GSE12790
| | Sample_data_row_count | 54675
| | |
|
GSM320252 | GPL570 |
|
MCF10a_HRas_Vector_rep4
|
MCF10a treated with HRas vector 24h
|
MCF10a immortalized, non-transformed human mammary epithelial cell-line overexpressing RAS
|
MCF10a_HRas_Vector_rep4
|
| Sample_geo_accession | GSM320252
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Sep 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were optimally transfected with null, HRAS or MEK1 vectors. Cells lysed 24h post-transfection
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM/F12 with 15 mM hepes buffer supplemented with 5% horse serum, 10 ug/ml insulin, 20 ng/ml EGF, 100 ng/ml choleratoxin, 0.5 ug/ml hydrocortisone pen/strep
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy kit used for the extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133P 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mark,,Lackner
| | Sample_contact_email | lackner.mark@gene.com
| | Sample_contact_institute | Genentech
| | Sample_contact_address | 1 DNA Way
| | Sample_contact_city | South San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94080
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320252/suppl/GSM320252.CEL.gz
| | Sample_series_id | GSE12764
| | Sample_series_id | GSE12790
| | Sample_data_row_count | 54675
| | |
|
GSM320253 | GPL570 |
|
MCF10a_HRas_Vector_rep5
|
MCF10a treated with HRas vector 24h
|
MCF10a immortalized, non-transformed human mammary epithelial cell-line overexpressing RAS
|
MCF10a_HRas_Vector_rep5
|
| Sample_geo_accession | GSM320253
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Sep 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were optimally transfected with null, HRAS or MEK1 vectors. Cells lysed 24h post-transfection
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM/F12 with 15 mM hepes buffer supplemented with 5% horse serum, 10 ug/ml insulin, 20 ng/ml EGF, 100 ng/ml choleratoxin, 0.5 ug/ml hydrocortisone pen/strep
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy kit used for the extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133P 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mark,,Lackner
| | Sample_contact_email | lackner.mark@gene.com
| | Sample_contact_institute | Genentech
| | Sample_contact_address | 1 DNA Way
| | Sample_contact_city | South San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94080
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320253/suppl/GSM320253.CEL.gz
| | Sample_series_id | GSE12764
| | Sample_series_id | GSE12790
| | Sample_data_row_count | 54675
| | |
|
GSM320254 | GPL570 |
|
MCF10a_MEK1_Vector_rep1
|
MCF10a treated with MEK1 vector 24h
|
MCF10a immortalized, non-transformed human mammary epithelial cell-line overexpressing MEK
|
MCF10a_MEK1_Vector_rep1
|
| Sample_geo_accession | GSM320254
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Sep 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were optimally transfected with null, HRAS or MEK1 vectors. Cells lysed 24h post-transfection
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM/F12 with 15 mM hepes buffer supplemented with 5% horse serum, 10 ug/ml insulin, 20 ng/ml EGF, 100 ng/ml choleratoxin, 0.5 ug/ml hydrocortisone pen/strep
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy kit used for the extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133P 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mark,,Lackner
| | Sample_contact_email | lackner.mark@gene.com
| | Sample_contact_institute | Genentech
| | Sample_contact_address | 1 DNA Way
| | Sample_contact_city | South San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94080
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320254/suppl/GSM320254.CEL.gz
| | Sample_series_id | GSE12764
| | Sample_series_id | GSE12790
| | Sample_data_row_count | 54675
| | |
|
GSM320255 | GPL570 |
|
MCF10a_MEK1_Vector_rep2
|
MCF10a treated with MEK1 vector 24h
|
MCF10a immortalized, non-transformed human mammary epithelial cell-line overexpressing MEK
|
MCF10a_MEK1_Vector_rep2
|
| Sample_geo_accession | GSM320255
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Sep 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were optimally transfected with null, HRAS or MEK1 vectors. Cells lysed 24h post-transfection
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM/F12 with 15 mM hepes buffer supplemented with 5% horse serum, 10 ug/ml insulin, 20 ng/ml EGF, 100 ng/ml choleratoxin, 0.5 ug/ml hydrocortisone pen/strep
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy kit used for the extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133P 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mark,,Lackner
| | Sample_contact_email | lackner.mark@gene.com
| | Sample_contact_institute | Genentech
| | Sample_contact_address | 1 DNA Way
| | Sample_contact_city | South San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94080
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320255/suppl/GSM320255.CEL.gz
| | Sample_series_id | GSE12764
| | Sample_series_id | GSE12790
| | Sample_data_row_count | 54675
| | |
|
GSM320256 | GPL570 |
|
MCF10a_MEK1_Vector_rep3
|
MCF10a treated with MEK1 vector 24h
|
MCF10a immortalized, non-transformed human mammary epithelial cell-line overexpressing MEK
|
MCF10a_MEK1_Vector_rep3
|
| Sample_geo_accession | GSM320256
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Sep 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were optimally transfected with null, HRAS or MEK1 vectors. Cells lysed 24h post-transfection
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM/F12 with 15 mM hepes buffer supplemented with 5% horse serum, 10 ug/ml insulin, 20 ng/ml EGF, 100 ng/ml choleratoxin, 0.5 ug/ml hydrocortisone pen/strep
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy kit used for the extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133P 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mark,,Lackner
| | Sample_contact_email | lackner.mark@gene.com
| | Sample_contact_institute | Genentech
| | Sample_contact_address | 1 DNA Way
| | Sample_contact_city | South San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94080
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320256/suppl/GSM320256.CEL.gz
| | Sample_series_id | GSE12764
| | Sample_series_id | GSE12790
| | Sample_data_row_count | 54675
| | |
|
GSM320257 | GPL570 |
|
MCF10a_MEK1_Vector_rep4
|
MCF10a treated with MEK1 vector 24h
|
MCF10a immortalized, non-transformed human mammary epithelial cell-line overexpressing MEK
|
MCF10a_MEK1_Vector_rep4
|
| Sample_geo_accession | GSM320257
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Sep 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were optimally transfected with null, HRAS or MEK1 vectors. Cells lysed 24h post-transfection
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM/F12 with 15 mM hepes buffer supplemented with 5% horse serum, 10 ug/ml insulin, 20 ng/ml EGF, 100 ng/ml choleratoxin, 0.5 ug/ml hydrocortisone pen/strep
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy kit used for the extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133P 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mark,,Lackner
| | Sample_contact_email | lackner.mark@gene.com
| | Sample_contact_institute | Genentech
| | Sample_contact_address | 1 DNA Way
| | Sample_contact_city | South San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94080
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320257/suppl/GSM320257.CEL.gz
| | Sample_series_id | GSE12764
| | Sample_series_id | GSE12790
| | Sample_data_row_count | 54675
| | |
|
GSM320258 | GPL570 |
|
MCF10a_MEK1_Vector_rep5
|
MCF10a treated with MEK1 vector 24h
|
MCF10a immortalized, non-transformed human mammary epithelial cell-line overexpressing MEK
|
MCF10a_MEK1_Vector_rep5
|
| Sample_geo_accession | GSM320258
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Sep 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were optimally transfected with null, HRAS or MEK1 vectors. Cells lysed 24h post-transfection
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM/F12 with 15 mM hepes buffer supplemented with 5% horse serum, 10 ug/ml insulin, 20 ng/ml EGF, 100 ng/ml choleratoxin, 0.5 ug/ml hydrocortisone pen/strep
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy kit used for the extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133P 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mark,,Lackner
| | Sample_contact_email | lackner.mark@gene.com
| | Sample_contact_institute | Genentech
| | Sample_contact_address | 1 DNA Way
| | Sample_contact_city | South San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94080
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320258/suppl/GSM320258.CEL.gz
| | Sample_series_id | GSE12764
| | Sample_series_id | GSE12790
| | Sample_data_row_count | 54675
| | |
|
GSM320259 | GPL570 |
|
MCF10a_MEK1_Vector_rep6
|
MCF10a treated with MEK1 vector 24h
|
MCF10a immortalized, non-transformed human mammary epithelial cell-line overexpressing MEK
|
MCF10a_MEK1_Vector_rep6
|
| Sample_geo_accession | GSM320259
| | Sample_status | Public on Jan 12 2009
| | Sample_submission_date | Sep 12 2008
| | Sample_last_update_date | Sep 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were optimally transfected with null, HRAS or MEK1 vectors. Cells lysed 24h post-transfection
| | Sample_growth_protocol_ch1 | Cells were grown in DMEM/F12 with 15 mM hepes buffer supplemented with 5% horse serum, 10 ug/ml insulin, 20 ng/ml EGF, 100 ng/ml choleratoxin, 0.5 ug/ml hydrocortisone pen/strep
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen Rneasy kit used for the extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133P 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mark,,Lackner
| | Sample_contact_email | lackner.mark@gene.com
| | Sample_contact_institute | Genentech
| | Sample_contact_address | 1 DNA Way
| | Sample_contact_city | South San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94080
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM320nnn/GSM320259/suppl/GSM320259.CEL.gz
| | Sample_series_id | GSE12764
| | Sample_series_id | GSE12790
| | Sample_data_row_count | 54675
| | |
|
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