Search results for the GEO ID: GSE12799  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM321540 | GPL85 |
|
Myometrium late pregnant Sample 1
|
Myometrium late pregnant
|
Strain: Wistar, Female, 3 month old, 21 days
|
Uteri were dissected from late pregnant rats (21 days, LP) (three individuals, two replicates of sample 1) and rats during labor (DL) after the expulsion of the second pup (three individuals, two replicates of sample 1).
|
| Sample_geo_accession | GSM321540
| | Sample_status | Public on Sep 23 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After dissection, uteri were placed immediately in cold Ca2+-free PBS buffer and peripheral large blood vessels, external surrounding connective tissue and placenta were removed. The endometrium superficial layer was separated by gentle scraping. The myometrium was weighted, finely minced and homogenized with a polytron (Polytron PT3000, Brinkmann, Switzerland). To extract total RNA the Ambion Totally RNA kit (Ambion, Austin, TX) was used. RNA concentration was determined with a spectrophotometer at 260 nm OD. The RNA purity was assessed by the OD ratio 260 nm/280 nm and the RNA integrity by the comparison of the 28S and 18S bands from 1 ug total RNA in 0.8% agarose gels in Tris-borate EDTA buffer with 0.5ug/ml ethydium bromide.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized with the SuperScript Choice System (Life Technologies, Grand Island, NY) using an oligo-dT primer containing the sequence of the T7 promoter region (Genset, La Jolla, CA). The cDNA was then used to perform in vitro transcription incorporating biotin-labeled nucleotides with the Enzo BioArray kit (Enzo Biochem, CA).
| | Sample_hyb_protocol | cRNA biotin-labeled was fragmented and hybridized to Affymetrix Rat RG U34-A GeneChip containing 8740 probe sets targeting different sequences.
| | Sample_scan_protocol | The arrays were washed, stained, and scanned with Fluidics Station 400 (Affymetrix, Santa Clara, CA) and Agilent Scanner (Hewlett–Packard, USA).
| | Sample_data_processing | To determine the presence or absence for each sequence in the experimental cRNA, microarray data were initially processed with Affymetrix Microarray suite 5.0. Since microarrays may differ in their hybridization patterns, to quantify cRNA sequence expression levels, the values of each cell intensity was calculated using the Li and Wong statistical model applied to all the microarrays (dChip software)
| | Sample_platform_id | GPL85
| | Sample_contact_name | Enrico,,Stefani
| | Sample_contact_laboratory | Stefani's lab
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | David Geffen School of Medicine at UCLA
| | Sample_contact_address | 650 Charles Young Dr
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321540/suppl/GSM321540.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321540/suppl/GSM321540.CHP.gz
| | Sample_series_id | GSE12799
| | Sample_data_row_count | 8799
| | |
|
GSM321541 | GPL85 |
|
Myometrium Late Pregnant Rep. 1 of Sample 1
|
Myometrium Late Pregnant Rep. 1 of Sample 1
|
Strain: Wistar, female 3 month old, 21 days late pregnant
|
Uteri were dissected from late pregnant rats (21 days, LP) (three individuals, two replicates of sample 1) and rats during labor (DL) after the expulsion of the second pup (three individuals, two replicates of sample 1).
|
| Sample_geo_accession | GSM321541
| | Sample_status | Public on Sep 23 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After dissection, uteri were placed immediately in cold Ca2+-free PBS buffer and peripheral large blood vessels, external surrounding connective tissue and placenta were removed. The endometrium superficial layer was separated by gentle scraping. The myometrium was weighted, finely minced and homogenized with a polytron (Polytron PT3000, Brinkmann, Switzerland). To extract total RNA the Ambion Totally RNA kit (Ambion, Austin, TX) was used. RNA concentration was determined with a spectrophotometer at 260 nm OD. The RNA purity was assessed by the OD ratio 260 nm/280 nm and the RNA integrity by the comparison of the 28S and 18S bands from 1 ug total RNA in 0.8% agarose gels in Tris-borate EDTA buffer with 0.5ug/ml ethydium bromide.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized with the SuperScript Choice System (Life Technologies, Grand Island, NY) using an oligo-dT primer containing the sequence of the T7 promoter region (Genset, La Jolla, CA). The cDNA was then used to perform in vitro transcription incorporating biotin-labeled nucleotides with the Enzo BioArray kit (Enzo Biochem, CA).
| | Sample_hyb_protocol | cRNA biotin-labeled was fragmented and hybridized to Affymetrix Rat RG U34-A GeneChip containing 8740 probe sets targeting different sequences.
| | Sample_scan_protocol | The arrays were washed, stained, and scanned with Fluidics Station 400 (Affymetrix, Santa Clara, CA) and Agilent Scanner (Hewlett–Packard, USA).
| | Sample_data_processing | To determine the presence or absence for each sequence in the experimental cRNA, microarray data were initially processed with Affymetrix Microarray suite 5.0. Since microarrays may differ in their hybridization patterns, to quantify cRNA sequence expression levels, the values of each cell intensity was calculated using the Li and Wong statistical model applied to all the microarrays (dChip software)
| | Sample_platform_id | GPL85
| | Sample_contact_name | Enrico,,Stefani
| | Sample_contact_laboratory | Stefani's lab
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | David Geffen School of Medicine at UCLA
| | Sample_contact_address | 650 Charles Young Dr
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321541/suppl/GSM321541.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321541/suppl/GSM321541.CHP.gz
| | Sample_series_id | GSE12799
| | Sample_data_row_count | 8799
| | |
|
GSM321542 | GPL85 |
|
Myometrium Late Pregnant Rep. 2 of Sample 1
|
Myometrium Late Pregnant Rep. 2 of Sample 1
|
Strain: Wistar, female 3 month old, 21 days late pregnant
|
Uteri were dissected from late pregnant rats (21 days, LP) (three individuals, two replicates of sample 1) and rats during labor (DL) after the expulsion of the second pup (three individuals, two replicates of sample 1).
|
| Sample_geo_accession | GSM321542
| | Sample_status | Public on Sep 23 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After dissection, uteri were placed immediately in cold Ca2+-free PBS buffer and peripheral large blood vessels, external surrounding connective tissue and placenta were removed. The endometrium superficial layer was separated by gentle scraping. The myometrium was weighted, finely minced and homogenized with a polytron (Polytron PT3000, Brinkmann, Switzerland). To extract total RNA the Ambion Totally RNA kit (Ambion, Austin, TX) was used. RNA concentration was determined with a spectrophotometer at 260 nm OD. The RNA purity was assessed by the OD ratio 260 nm/280 nm and the RNA integrity by the comparison of the 28S and 18S bands from 1 ug total RNA in 0.8% agarose gels in Tris-borate EDTA buffer with 0.5ug/ml ethydium bromide.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized with the SuperScript Choice System (Life Technologies, Grand Island, NY) using an oligo-dT primer containing the sequence of the T7 promoter region (Genset, La Jolla, CA). The cDNA was then used to perform in vitro transcription incorporating biotin-labeled nucleotides with the Enzo BioArray kit (Enzo Biochem, CA).
| | Sample_hyb_protocol | cRNA biotin-labeled was fragmented and hybridized to Affymetrix Rat RG U34-A GeneChip containing 8740 probe sets targeting different sequences.
| | Sample_scan_protocol | The arrays were washed, stained, and scanned with Fluidics Station 400 (Affymetrix, Santa Clara, CA) and Agilent Scanner (Hewlett–Packard, USA).
| | Sample_data_processing | To determine the presence or absence for each sequence in the experimental cRNA, microarray data were initially processed with Affymetrix Microarray suite 5.0. Since microarrays may differ in their hybridization patterns, to quantify cRNA sequence expression levels, the values of each cell intensity was calculated using the Li and Wong statistical model applied to all the microarrays (dChip software)
| | Sample_platform_id | GPL85
| | Sample_contact_name | Enrico,,Stefani
| | Sample_contact_laboratory | Stefani's lab
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | David Geffen School of Medicine at UCLA
| | Sample_contact_address | 650 Charles Young Dr
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321542/suppl/GSM321542.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321542/suppl/GSM321542.CHP.gz
| | Sample_series_id | GSE12799
| | Sample_data_row_count | 8799
| | |
|
GSM321543 | GPL85 |
|
Myometrium Late Pregnant Sample 2
|
Myometrium Late Pregnant Sample 2
|
Strain: Wistar, female 3 month old, 21 days late pregnant
|
Uteri were dissected from late pregnant rats (21 days, LP) (three individuals, two replicates of sample 1) and rats during labor (DL) after the expulsion of the second pup (three individuals, two replicates of sample 1).
|
| Sample_geo_accession | GSM321543
| | Sample_status | Public on Sep 23 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After dissection, uteri were placed immediately in cold Ca2+-free PBS buffer and peripheral large blood vessels, external surrounding connective tissue and placenta were removed. The endometrium superficial layer was separated by gentle scraping. The myometrium was weighted, finely minced and homogenized with a polytron (Polytron PT3000, Brinkmann, Switzerland). To extract total RNA the Ambion Totally RNA kit (Ambion, Austin, TX) was used. RNA concentration was determined with a spectrophotometer at 260 nm OD. The RNA purity was assessed by the OD ratio 260 nm/280 nm and the RNA integrity by the comparison of the 28S and 18S bands from 1 ug total RNA in 0.8% agarose gels in Tris-borate EDTA buffer with 0.5ug/ml ethydium bromide.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized with the SuperScript Choice System (Life Technologies, Grand Island, NY) using an oligo-dT primer containing the sequence of the T7 promoter region (Genset, La Jolla, CA). The cDNA was then used to perform in vitro transcription incorporating biotin-labeled nucleotides with the Enzo BioArray kit (Enzo Biochem, CA).
| | Sample_hyb_protocol | cRNA biotin-labeled was fragmented and hybridized to Affymetrix Rat RG U34-A GeneChip containing 8740 probe sets targeting different sequences.
| | Sample_scan_protocol | The arrays were washed, stained, and scanned with Fluidics Station 400 (Affymetrix, Santa Clara, CA) and Agilent Scanner (Hewlett–Packard, USA).
| | Sample_data_processing | To determine the presence or absence for each sequence in the experimental cRNA, microarray data were initially processed with Affymetrix Microarray suite 5.0. Since microarrays may differ in their hybridization patterns, to quantify cRNA sequence expression levels, the values of each cell intensity was calculated using the Li and Wong statistical model applied to all the microarrays (dChip software)
| | Sample_platform_id | GPL85
| | Sample_contact_name | Enrico,,Stefani
| | Sample_contact_laboratory | Stefani's lab
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | David Geffen School of Medicine at UCLA
| | Sample_contact_address | 650 Charles Young Dr
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321543/suppl/GSM321543.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321543/suppl/GSM321543.CHP.gz
| | Sample_series_id | GSE12799
| | Sample_data_row_count | 8799
| | |
|
GSM321544 | GPL85 |
|
Myometrium Late Pregnant Sample 3
|
Myometrium Late Pregnant Sample 3
|
Strain: Wistar, female 3 month old, 21 days late pregnant
|
Uteri were dissected from late pregnant rats (21 days, LP) (three individuals, two replicates of sample 1) and rats during labor (DL) after the expulsion of the second pup (three individuals, two replicates of sample 1).
|
| Sample_geo_accession | GSM321544
| | Sample_status | Public on Sep 23 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After dissection, uteri were placed immediately in cold Ca2+-free PBS buffer and peripheral large blood vessels, external surrounding connective tissue and placenta were removed. The endometrium superficial layer was separated by gentle scraping. The myometrium was weighted, finely minced and homogenized with a polytron (Polytron PT3000, Brinkmann, Switzerland). To extract total RNA the Ambion Totally RNA kit (Ambion, Austin, TX) was used. RNA concentration was determined with a spectrophotometer at 260 nm OD. The RNA purity was assessed by the OD ratio 260 nm/280 nm and the RNA integrity by the comparison of the 28S and 18S bands from 1 ug total RNA in 0.8% agarose gels in Tris-borate EDTA buffer with 0.5ug/ml ethydium bromide.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized with the SuperScript Choice System (Life Technologies, Grand Island, NY) using an oligo-dT primer containing the sequence of the T7 promoter region (Genset, La Jolla, CA). The cDNA was then used to perform in vitro transcription incorporating biotin-labeled nucleotides with the Enzo BioArray kit (Enzo Biochem, CA).
| | Sample_hyb_protocol | cRNA biotin-labeled was fragmented and hybridized to Affymetrix Rat RG U34-A GeneChip containing 8740 probe sets targeting different sequences.
| | Sample_scan_protocol | The arrays were washed, stained, and scanned with Fluidics Station 400 (Affymetrix, Santa Clara, CA) and Agilent Scanner (Hewlett–Packard, USA).
| | Sample_data_processing | To determine the presence or absence for each sequence in the experimental cRNA, microarray data were initially processed with Affymetrix Microarray suite 5.0. Since microarrays may differ in their hybridization patterns, to quantify cRNA sequence expression levels, the values of each cell intensity was calculated using the Li and Wong statistical model applied to all the microarrays (dChip software)
| | Sample_platform_id | GPL85
| | Sample_contact_name | Enrico,,Stefani
| | Sample_contact_laboratory | Stefani's lab
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | David Geffen School of Medicine at UCLA
| | Sample_contact_address | 650 Charles Young Dr
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321544/suppl/GSM321544.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321544/suppl/GSM321544.CHP.gz
| | Sample_series_id | GSE12799
| | Sample_data_row_count | 8799
| | |
|
GSM321545 | GPL85 |
|
Myometrium During Labor Sample 1
|
Myometrium During Labor Sample 1
|
Strain: Wistar, female 3 month old, During Labor after second pup
|
Uteri were dissected from late pregnant rats (21 days, LP) (three individuals, two replicates of sample 1) and rats during labor (DL) after the expulsion of the second pup (three individuals, two replicates of sample 1).
|
| Sample_geo_accession | GSM321545
| | Sample_status | Public on Sep 23 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After dissection, uteri were placed immediately in cold Ca2+-free PBS buffer and peripheral large blood vessels, external surrounding connective tissue and placenta were removed. The endometrium superficial layer was separated by gentle scraping. The myometrium was weighted, finely minced and homogenized with a polytron (Polytron PT3000, Brinkmann, Switzerland). To extract total RNA the Ambion Totally RNA kit (Ambion, Austin, TX) was used. RNA concentration was determined with a spectrophotometer at 260 nm OD. The RNA purity was assessed by the OD ratio 260 nm/280 nm and the RNA integrity by the comparison of the 28S and 18S bands from 1 ug total RNA in 0.8% agarose gels in Tris-borate EDTA buffer with 0.5ug/ml ethydium bromide.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized with the SuperScript Choice System (Life Technologies, Grand Island, NY) using an oligo-dT primer containing the sequence of the T7 promoter region (Genset, La Jolla, CA). The cDNA was then used to perform in vitro transcription incorporating biotin-labeled nucleotides with the Enzo BioArray kit (Enzo Biochem, CA).
| | Sample_hyb_protocol | cRNA biotin-labeled was fragmented and hybridized to Affymetrix Rat RG U34-A GeneChip containing 8740 probe sets targeting different sequences.
| | Sample_scan_protocol | The arrays were washed, stained, and scanned with Fluidics Station 400 (Affymetrix, Santa Clara, CA) and Agilent Scanner (Hewlett–Packard, USA).
| | Sample_data_processing | To determine the presence or absence for each sequence in the experimental cRNA, microarray data were initially processed with Affymetrix Microarray suite 5.0. Since microarrays may differ in their hybridization patterns, to quantify cRNA sequence expression levels, the values of each cell intensity was calculated using the Li and Wong statistical model applied to all the microarrays (dChip software)
| | Sample_platform_id | GPL85
| | Sample_contact_name | Enrico,,Stefani
| | Sample_contact_laboratory | Stefani's lab
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | David Geffen School of Medicine at UCLA
| | Sample_contact_address | 650 Charles Young Dr
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321545/suppl/GSM321545.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321545/suppl/GSM321545.CHP.gz
| | Sample_series_id | GSE12799
| | Sample_data_row_count | 8799
| | |
|
GSM321546 | GPL85 |
|
Myometrium During Labor Rep. 1 of Sample 1
|
Myometrium During Labor Rep. 1 of Sample 1
|
Strain: Wistar, female 3 month old, During Labor after second pup
|
Uteri were dissected from late pregnant rats (21 days, LP) (three individuals, two replicates of sample 1) and rats during labor (DL) after the expulsion of the second pup (three individuals, two replicates of sample 1).
|
| Sample_geo_accession | GSM321546
| | Sample_status | Public on Sep 23 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After dissection, uteri were placed immediately in cold Ca2+-free PBS buffer and peripheral large blood vessels, external surrounding connective tissue and placenta were removed. The endometrium superficial layer was separated by gentle scraping. The myometrium was weighted, finely minced and homogenized with a polytron (Polytron PT3000, Brinkmann, Switzerland). To extract total RNA the Ambion Totally RNA kit (Ambion, Austin, TX) was used. RNA concentration was determined with a spectrophotometer at 260 nm OD. The RNA purity was assessed by the OD ratio 260 nm/280 nm and the RNA integrity by the comparison of the 28S and 18S bands from 1 ug total RNA in 0.8% agarose gels in Tris-borate EDTA buffer with 0.5ug/ml ethydium bromide.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized with the SuperScript Choice System (Life Technologies, Grand Island, NY) using an oligo-dT primer containing the sequence of the T7 promoter region (Genset, La Jolla, CA). The cDNA was then used to perform in vitro transcription incorporating biotin-labeled nucleotides with the Enzo BioArray kit (Enzo Biochem, CA).
| | Sample_hyb_protocol | cRNA biotin-labeled was fragmented and hybridized to Affymetrix Rat RG U34-A GeneChip containing 8740 probe sets targeting different sequences.
| | Sample_scan_protocol | The arrays were washed, stained, and scanned with Fluidics Station 400 (Affymetrix, Santa Clara, CA) and Agilent Scanner (Hewlett–Packard, USA).
| | Sample_data_processing | To determine the presence or absence for each sequence in the experimental cRNA, microarray data were initially processed with Affymetrix Microarray suite 5.0. Since microarrays may differ in their hybridization patterns, to quantify cRNA sequence expression levels, the values of each cell intensity was calculated using the Li and Wong statistical model applied to all the microarrays (dChip software)
| | Sample_platform_id | GPL85
| | Sample_contact_name | Enrico,,Stefani
| | Sample_contact_laboratory | Stefani's lab
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | David Geffen School of Medicine at UCLA
| | Sample_contact_address | 650 Charles Young Dr
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321546/suppl/GSM321546.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321546/suppl/GSM321546.CHP.gz
| | Sample_series_id | GSE12799
| | Sample_data_row_count | 8799
| | |
|
GSM321547 | GPL85 |
|
Myometrium During Labor Rep. 2 of Sample 1
|
Myometrium During Labor Rep. 2 of Sample 1
|
Strain: Wistar, female 3 month old, During Labor after second pup
|
Uteri were dissected from late pregnant rats (21 days, LP) (three individuals, two replicates of sample 1) and rats during labor (DL) after the expulsion of the second pup (three individuals, two replicates of sample 1).
|
| Sample_geo_accession | GSM321547
| | Sample_status | Public on Sep 23 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After dissection, uteri were placed immediately in cold Ca2+-free PBS buffer and peripheral large blood vessels, external surrounding connective tissue and placenta were removed. The endometrium superficial layer was separated by gentle scraping. The myometrium was weighted, finely minced and homogenized with a polytron (Polytron PT3000, Brinkmann, Switzerland). To extract total RNA the Ambion Totally RNA kit (Ambion, Austin, TX) was used. RNA concentration was determined with a spectrophotometer at 260 nm OD. The RNA purity was assessed by the OD ratio 260 nm/280 nm and the RNA integrity by the comparison of the 28S and 18S bands from 1 ug total RNA in 0.8% agarose gels in Tris-borate EDTA buffer with 0.5ug/ml ethydium bromide.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized with the SuperScript Choice System (Life Technologies, Grand Island, NY) using an oligo-dT primer containing the sequence of the T7 promoter region (Genset, La Jolla, CA). The cDNA was then used to perform in vitro transcription incorporating biotin-labeled nucleotides with the Enzo BioArray kit (Enzo Biochem, CA).
| | Sample_hyb_protocol | cRNA biotin-labeled was fragmented and hybridized to Affymetrix Rat RG U34-A GeneChip containing 8740 probe sets targeting different sequences.
| | Sample_scan_protocol | The arrays were washed, stained, and scanned with Fluidics Station 400 (Affymetrix, Santa Clara, CA) and Agilent Scanner (Hewlett–Packard, USA).
| | Sample_data_processing | To determine the presence or absence for each sequence in the experimental cRNA, microarray data were initially processed with Affymetrix Microarray suite 5.0. Since microarrays may differ in their hybridization patterns, to quantify cRNA sequence expression levels, the values of each cell intensity was calculated using the Li and Wong statistical model applied to all the microarrays (dChip software)
| | Sample_platform_id | GPL85
| | Sample_contact_name | Enrico,,Stefani
| | Sample_contact_laboratory | Stefani's lab
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | David Geffen School of Medicine at UCLA
| | Sample_contact_address | 650 Charles Young Dr
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321547/suppl/GSM321547.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321547/suppl/GSM321547.CHP.gz
| | Sample_series_id | GSE12799
| | Sample_data_row_count | 8799
| | |
|
GSM321548 | GPL85 |
|
Myometrium During Labor Sample 2
|
Myometrium During Labor Sample 2
|
Strain: Wistar, female 3 month old, During Labor after second pup
|
Uteri were dissected from late pregnant rats (21 days, LP) (three individuals, two replicates of sample 1) and rats during labor (DL) after the expulsion of the second pup (three individuals, two replicates of sample 1).
|
| Sample_geo_accession | GSM321548
| | Sample_status | Public on Sep 23 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After dissection, uteri were placed immediately in cold Ca2+-free PBS buffer and peripheral large blood vessels, external surrounding connective tissue and placenta were removed. The endometrium superficial layer was separated by gentle scraping. The myometrium was weighted, finely minced and homogenized with a polytron (Polytron PT3000, Brinkmann, Switzerland). To extract total RNA the Ambion Totally RNA kit (Ambion, Austin, TX) was used. RNA concentration was determined with a spectrophotometer at 260 nm OD. The RNA purity was assessed by the OD ratio 260 nm/280 nm and the RNA integrity by the comparison of the 28S and 18S bands from 1 ug total RNA in 0.8% agarose gels in Tris-borate EDTA buffer with 0.5ug/ml ethydium bromide.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized with the SuperScript Choice System (Life Technologies, Grand Island, NY) using an oligo-dT primer containing the sequence of the T7 promoter region (Genset, La Jolla, CA). The cDNA was then used to perform in vitro transcription incorporating biotin-labeled nucleotides with the Enzo BioArray kit (Enzo Biochem, CA).
| | Sample_hyb_protocol | cRNA biotin-labeled was fragmented and hybridized to Affymetrix Rat RG U34-A GeneChip containing 8740 probe sets targeting different sequences.
| | Sample_scan_protocol | The arrays were washed, stained, and scanned with Fluidics Station 400 (Affymetrix, Santa Clara, CA) and Agilent Scanner (Hewlett–Packard, USA).
| | Sample_data_processing | To determine the presence or absence for each sequence in the experimental cRNA, microarray data were initially processed with Affymetrix Microarray suite 5.0. Since microarrays may differ in their hybridization patterns, to quantify cRNA sequence expression levels, the values of each cell intensity was calculated using the Li and Wong statistical model applied to all the microarrays (dChip software)
| | Sample_platform_id | GPL85
| | Sample_contact_name | Enrico,,Stefani
| | Sample_contact_laboratory | Stefani's lab
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | David Geffen School of Medicine at UCLA
| | Sample_contact_address | 650 Charles Young Dr
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321548/suppl/GSM321548.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321548/suppl/GSM321548.CHP.gz
| | Sample_series_id | GSE12799
| | Sample_data_row_count | 8799
| | |
|
GSM321549 | GPL85 |
|
Myometrium During Labor Sample 3
|
Myometrium During Labor Sample 3
|
Strain: Wistar, female 3 month old, During Labor after second pup
|
Uteri were dissected from late pregnant rats (21 days, LP) (three individuals, two replicates of sample 1) and rats during labor (DL) after the expulsion of the second pup (three individuals, two replicates of sample 1).
|
| Sample_geo_accession | GSM321549
| | Sample_status | Public on Sep 23 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 22 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | After dissection, uteri were placed immediately in cold Ca2+-free PBS buffer and peripheral large blood vessels, external surrounding connective tissue and placenta were removed. The endometrium superficial layer was separated by gentle scraping. The myometrium was weighted, finely minced and homogenized with a polytron (Polytron PT3000, Brinkmann, Switzerland). To extract total RNA the Ambion Totally RNA kit (Ambion, Austin, TX) was used. RNA concentration was determined with a spectrophotometer at 260 nm OD. The RNA purity was assessed by the OD ratio 260 nm/280 nm and the RNA integrity by the comparison of the 28S and 18S bands from 1 ug total RNA in 0.8% agarose gels in Tris-borate EDTA buffer with 0.5ug/ml ethydium bromide.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized with the SuperScript Choice System (Life Technologies, Grand Island, NY) using an oligo-dT primer containing the sequence of the T7 promoter region (Genset, La Jolla, CA). The cDNA was then used to perform in vitro transcription incorporating biotin-labeled nucleotides with the Enzo BioArray kit (Enzo Biochem, CA).
| | Sample_hyb_protocol | cRNA biotin-labeled was fragmented and hybridized to Affymetrix Rat RG U34-A GeneChip containing 8740 probe sets targeting different sequences.
| | Sample_scan_protocol | The arrays were washed, stained, and scanned with Fluidics Station 400 (Affymetrix, Santa Clara, CA) and Agilent Scanner (Hewlett–Packard, USA).
| | Sample_data_processing | To determine the presence or absence for each sequence in the experimental cRNA, microarray data were initially processed with Affymetrix Microarray suite 5.0. Since microarrays may differ in their hybridization patterns, to quantify cRNA sequence expression levels, the values of each cell intensity was calculated using the Li and Wong statistical model applied to all the microarrays (dChip software)
| | Sample_platform_id | GPL85
| | Sample_contact_name | Enrico,,Stefani
| | Sample_contact_laboratory | Stefani's lab
| | Sample_contact_department | Anesthesiology
| | Sample_contact_institute | David Geffen School of Medicine at UCLA
| | Sample_contact_address | 650 Charles Young Dr
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90095
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321549/suppl/GSM321549.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321549/suppl/GSM321549.CHP.gz
| | Sample_series_id | GSE12799
| | Sample_data_row_count | 8799
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
