Search results for the GEO ID: GSE12803  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM321567 | GPL96 |
|
CD34+_BM_1
|
Stem/progenitor hematopoietic cells CD34+ from bone marrow (BM)
|
type: primary cells from healthy donors
|
Stem/progenitor hematopoietic cells CD34+ from bone marrow (BM)
|
| Sample_geo_accession | GSM321567
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Human CD34+ cells were purified from bone marrow blood samples: mononuclear cells were isolated by Ficoll-Hypaque gradient separation, washed twice with PBS, and then CD34+ cells separated using a magnetic cell sorting procedure (EasySep Human CD34 Positive Selection Kit, StemCell Technologies).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321567/suppl/GSM321567.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
| | |
|
GSM321568 | GPL96 |
|
CD34+_BM_2
|
Stem/progenitor hematopoietic cells CD34+ from bone marrow (BM)
|
type: primary cells from healthy donors
|
Stem/progenitor hematopoietic cells CD34+ from bone marrow (BM)
|
| Sample_geo_accession | GSM321568
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Human CD34+ cells were purified from bone marrow blood samples: mononuclear cells were isolated by Ficoll-Hypaque gradient separation, washed twice with PBS, and then CD34+ cells separated using a magnetic cell sorting procedure (EasySep Human CD34 Positive Selection Kit, StemCell Technologies).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321568/suppl/GSM321568.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
| | |
|
GSM321569 | GPL96 |
|
CD34+_CB_1
|
Stem/progenitor hematopoietic cells CD34+ from cord blood (CB)
|
type: primary cells from healthy donors
|
Stem/progenitor hematopoietic cells CD34+ from cord blood (CB)
|
| Sample_geo_accession | GSM321569
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Human CD34+ cells were purified from umbilical cord blood samples: mononuclear cells were isolated by Ficoll-Hypaque gradient separation, washed twice with PBS, and then CD34+ cells separated using a magnetic cell sorting procedure (EasySep Human CD34 Positive Selection Kit, StemCell Technologies).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321569/suppl/GSM321569.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
| | |
|
GSM321570 | GPL96 |
|
CD34+_CB_2
|
Stem/progenitor hematopoietic cells CD34+ from cord blood (CB)
|
type: primary cells from healthy donors
|
Stem/progenitor hematopoietic cells CD34+ from cord blood (CB)
|
| Sample_geo_accession | GSM321570
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Human CD34+ cells were purified from umbilical cord blood samples: mononuclear cells were isolated by Ficoll-Hypaque gradient separation, washed twice with PBS, and then CD34+ cells separated using a magnetic cell sorting procedure (EasySep Human CD34 Positive Selection Kit, StemCell Technologies).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321570/suppl/GSM321570.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
| | |
|
GSM321571 | GPL96 |
|
CD34+_PB_1
|
Stem/progenitor hematopoietic cells CD34+ from periferal blood (PB)
|
type: primary cells from healthy donors
|
Stem/progenitor hematopoietic cells CD34+ from periferal blood (PB)
|
| Sample_geo_accession | GSM321571
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Peripheral blood hemopoietic stem and progenitor cells, were obtained from healthy donors who received recombinant human G-CSF (Lenograstim, Rhone-Poulenc Rorer, Milan, Italy), administered subcutaneously at 10 ug/kg per day for 5-6 days. Hematopoietic stem/progenitor CD34+ cell purification was then performed as described for cord blood samples: mononuclear cells were isolated by Ficoll-Hypaque gradient separation, washed twice with PBS, and then CD34+ cells separated using a magnetic cell sorting procedure (EasySep Human CD34 Positive Selection Kit, StemCell Technologies).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321571/suppl/GSM321571.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
| | |
|
GSM321572 | GPL96 |
|
CD34+_PB_2
|
Stem/progenitor hematopoietic cells CD34+ from periferal blood (PB)
|
type: primary cells from healthy donors
|
Stem/progenitor hematopoietic cells CD34+ from periferal blood (PB)
|
| Sample_geo_accession | GSM321572
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Peripheral blood hemopoietic stem and progenitor cells, were obtained from healthy donors who received recombinant human G-CSF (Lenograstim, Rhone-Poulenc Rorer, Milan, Italy), administered subcutaneously at 10 ug/kg per day for 5-6 days. Hematopoietic stem/progenitor CD34+ cell purification was then performed as described for cord blood samples: mononuclear cells were isolated by Ficoll-Hypaque gradient separation, washed twice with PBS, and then CD34+ cells separated using a magnetic cell sorting procedure (EasySep Human CD34 Positive Selection Kit, StemCell Technologies).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321572/suppl/GSM321572.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
| | |
|
GSM321573 | GPL96 |
|
Eosinophils_1
|
Normal human eosinophils
|
type: primary cells from healthy donors
|
Normal human eosinophils
|
| Sample_geo_accession | GSM321573
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Human granulocytes were initially collected from cell pellets obtained by Ficoll separation of peripheral blood samples. Erythrocytes contained in cell pellets were removed by means of osmotic lysis. Neutrophils (CD16+ fraction) and Eosinophils (CD16- fraction) were then purified using magnetic microbeads conjugated to mouse monoclonal anti-human CD16 Ab (Miltenyi, Auburn, CA)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321573/suppl/GSM321573.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
| | |
|
GSM321574 | GPL96 |
|
Erythroblasts_1
|
In vitro differentiated normal human erythroblasts
|
type: in vitro differentiated cells derived from CD34+ stem/progenitor cells from healthy donors
|
In vitro differentiated normal human erythroblasts
|
| Sample_geo_accession | GSM321574
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Normal human erythroblasts were obtained from cord blood CD34+ hematopoietic progenitors cultured in IMDM (Euroclone) supplemented with 20% BIT (Stem Cell Technologies), 50 ng/ml SCF and 4 U/ml Erythropoietin (R&D Systems, Minneapolis, MN) for 8-10 days[63]. Differentiation of CD34+ cells was monitored daily by morphological analysis of May and Grunwald - Giemsa (MGG) stained cytospins and by flow-cytometric analysis of glycophorin A (GPA) surface antigen expression, using the phycoerythrin (PE)-conjugated mouse anti-human GPA monoclonal antibody (MoAb) (Becton Dickinson Systems, Mountain View, CA, USA).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321574/suppl/GSM321574.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
| | |
|
GSM321575 | GPL96 |
|
Erythroblasts_2
|
In vitro differentiated normal human erythroblasts
|
type: in vitro differentiated cells derived from CD34+ stem/progenitor cells from healthy donors
|
In vitro differentiated normal human erythroblasts
|
| Sample_geo_accession | GSM321575
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Normal human erythroblasts were obtained from cord blood CD34+ hematopoietic progenitors cultured in IMDM (Euroclone) supplemented with 20% BIT (Stem Cell Technologies), 50 ng/ml SCF and 4 U/ml Erythropoietin (R&D Systems, Minneapolis, MN) for 8-10 days[63]. Differentiation of CD34+ cells was monitored daily by morphological analysis of May and Grunwald - Giemsa (MGG) stained cytospins and by flow-cytometric analysis of glycophorin A (GPA) surface antigen expression, using the phycoerythrin (PE)-conjugated mouse anti-human GPA monoclonal antibody (MoAb) (Becton Dickinson Systems, Mountain View, CA, USA).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321575/suppl/GSM321575.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
| | |
|
GSM321576 | GPL96 |
|
Erythroblasts_3
|
In vitro differentiated normal human erythroblasts
|
type: in vitro differentiated cells derived from CD34+ stem/progenitor cells from healthy donors
|
In vitro differentiated normal human erythroblasts
|
| Sample_geo_accession | GSM321576
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Normal human erythroblasts were obtained from cord blood CD34+ hematopoietic progenitors cultured in IMDM (Euroclone) supplemented with 20% BIT (Stem Cell Technologies), 50 ng/ml SCF and 4 U/ml Erythropoietin (R&D Systems, Minneapolis, MN) for 8-10 days[63]. Differentiation of CD34+ cells was monitored daily by morphological analysis of May and Grunwald - Giemsa (MGG) stained cytospins and by flow-cytometric analysis of glycophorin A (GPA) surface antigen expression, using the phycoerythrin (PE)-conjugated mouse anti-human GPA monoclonal antibody (MoAb) (Becton Dickinson Systems, Mountain View, CA, USA).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321576/suppl/GSM321576.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
| | |
|
GSM321577 | GPL96 |
|
Megakaryoblasts_1
|
In vitro differentiated normal human megakaryoblasts
|
type: in vitro differentiated cells derived from CD34+ stem/progenitor cells from healthy donors
|
In vitro differentiated normal human megakaryoblasts
|
| Sample_geo_accession | GSM321577
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Megakaryoblasts were obtained by in vitro differentiation of CD34+ cells performed as already described by Tenedini et al. Briefly, megakaryocytes were obtained from CD34+ cells cultivated in serum-free medium supplemented with 50 ng/mL SCF and 100ng/mL thrombopoietin (TPO; Genzyme, Boston, MA) for 14-16 days and subsequently selected by means of a magnetic beads sorting procedure using monoclonal antibody directed against CD41a antigen (Dako, Milan, Italy).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321577/suppl/GSM321577.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
| | |
|
GSM321578 | GPL96 |
|
Megakaryoblasts_2
|
In vitro differentiated normal human megakaryoblasts
|
type: in vitro differentiated cells derived from CD34+ stem/progenitor cells from healthy donors
|
In vitro differentiated normal human megakaryoblasts
|
| Sample_geo_accession | GSM321578
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Megakaryoblasts were obtained by in vitro differentiation of CD34+ cells performed as already described by Tenedini et al. Briefly, megakaryocytes were obtained from CD34+ cells cultivated in serum-free medium supplemented with 50 ng/mL SCF and 100ng/mL thrombopoietin (TPO; Genzyme, Boston, MA) for 14-16 days and subsequently selected by means of a magnetic beads sorting procedure using monoclonal antibody directed against CD41a antigen (Dako, Milan, Italy).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321578/suppl/GSM321578.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
| | |
|
GSM321579 | GPL96 |
|
Monoblasts_1
|
In vitro differentiated normal human monoblasts
|
type: in vitro differentiated cells derived from CD34+ stem/progenitor cells from healthy donors
|
In vitro differentiated normal human monoblasts
|
| Sample_geo_accession | GSM321579
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Monoblasts (CD14+ precursors) and myeloblasts (CD14- precursors) were obtained by in vitro differentiation of cord blood (CB) derived CD34+ cells performed as already described by Montanari et al. Briefly, CB CD34+ cells were cultured in IMDM added with 20% FCS (Bio-Whittaker, Walkersville, MD, USA), in the presence of human hematopoietic cytokines: SCF (50 ng/ml), Flt3-ligand (Flt3-l) (50 ng/ml), IL-11 (50 ng/ml), IL-6 (10 ng/ml), IL-3 (10 ng/ml) and G-CSF (10 ng/ml) (all from R&D Systems, Minneapolis, MN, USA). After 7 days of culture, hematopoietic cells were analyzed, by flow cytometry, for CD14 antigen expression, estimated at about 25-30% of the entire cell population. Then monoblasts (CD14+) and myeloblasts (CD14-) cell fractions were obtained by immunomagnetic separation using the MACS technology (Miltenyi). Differentiation of CD34+ cells was monitored by morphological analysis of MGG-stained cytospins and by flow-cytometric analysis of CD34, CD38 and CD14 surface antigen expression.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321579/suppl/GSM321579.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
| | |
|
GSM321580 | GPL96 |
|
Monoblasts_2
|
In vitro differentiated normal human monoblasts
|
type: in vitro differentiated cells derived from CD34+ stem/progenitor cells from healthy donors
|
In vitro differentiated normal human monoblasts
|
| Sample_geo_accession | GSM321580
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Monoblasts (CD14+ precursors) and myeloblasts (CD14- precursors) were obtained by in vitro differentiation of cord blood (CB) derived CD34+ cells performed as already described by Montanari et al. Briefly, CB CD34+ cells were cultured in IMDM added with 20% FCS (Bio-Whittaker, Walkersville, MD, USA), in the presence of human hematopoietic cytokines: SCF (50 ng/ml), Flt3-ligand (Flt3-l) (50 ng/ml), IL-11 (50 ng/ml), IL-6 (10 ng/ml), IL-3 (10 ng/ml) and G-CSF (10 ng/ml) (all from R&D Systems, Minneapolis, MN, USA). After 7 days of culture, hematopoietic cells were analyzed, by flow cytometry, for CD14 antigen expression, estimated at about 25-30% of the entire cell population. Then monoblasts (CD14+) and myeloblasts (CD14-) cell fractions were obtained by immunomagnetic separation using the MACS technology (Miltenyi). Differentiation of CD34+ cells was monitored by morphological analysis of MGG-stained cytospins and by flow-cytometric analysis of CD34, CD38 and CD14 surface antigen expression.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321580/suppl/GSM321580.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
| | |
|
GSM321581 | GPL96 |
|
Monoblasts_3
|
In vitro differentiated normal human monoblasts
|
type: in vitro differentiated cells derived from CD34+ stem/progenitor cells from healthy donors
|
In vitro differentiated normal human monoblasts
|
| Sample_geo_accession | GSM321581
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Monoblasts (CD14+ precursors) and myeloblasts (CD14- precursors) were obtained by in vitro differentiation of cord blood (CB) derived CD34+ cells performed as already described by Montanari et al. Briefly, CB CD34+ cells were cultured in IMDM added with 20% FCS (Bio-Whittaker, Walkersville, MD, USA), in the presence of human hematopoietic cytokines: SCF (50 ng/ml), Flt3-ligand (Flt3-l) (50 ng/ml), IL-11 (50 ng/ml), IL-6 (10 ng/ml), IL-3 (10 ng/ml) and G-CSF (10 ng/ml) (all from R&D Systems, Minneapolis, MN, USA). After 7 days of culture, hematopoietic cells were analyzed, by flow cytometry, for CD14 antigen expression, estimated at about 25-30% of the entire cell population. Then monoblasts (CD14+) and myeloblasts (CD14-) cell fractions were obtained by immunomagnetic separation using the MACS technology (Miltenyi). Differentiation of CD34+ cells was monitored by morphological analysis of MGG-stained cytospins and by flow-cytometric analysis of CD34, CD38 and CD14 surface antigen expression.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321581/suppl/GSM321581.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
| | |
|
GSM321582 | GPL96 |
|
Monocytes_1
|
Normal human monocytes
|
type: primary cells from healthy donors
|
Normal human monocytes
|
| Sample_geo_accession | GSM321582
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Normal human monocytes were selected from the Ficoll separated peripheral blood (PB) mononuclear cells of adult samples by means of magnetic microbeads conjugated with mouse monoclonal (Mo) anti-human CD14 antibody (Ab) (Miltenyi, Auburn, CA)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321582/suppl/GSM321582.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
| | |
|
GSM321583 | GPL96 |
|
Myeloblasts_1
|
In vitro differentiated normal human myeloblasts
|
type: in vitro differentiated cells derived from CD34+ stem/progenitor cells from healthy donors
|
In vitro differentiated normal human myeloblasts
|
| Sample_geo_accession | GSM321583
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Monoblasts (CD14+ precursors) and myeloblasts (CD14- precursors) were obtained by in vitro differentiation of cord blood (CB) derived CD34+ cells performed as already described by Montanari et al. Briefly, CB CD34+ cells were cultured in IMDM added with 20% FCS (Bio-Whittaker, Walkersville, MD, USA), in the presence of human hematopoietic cytokines: SCF (50 ng/ml), Flt3-ligand (Flt3-l) (50 ng/ml), IL-11 (50 ng/ml), IL-6 (10 ng/ml), IL-3 (10 ng/ml) and G-CSF (10 ng/ml) (all from R&D Systems, Minneapolis, MN, USA). After 7 days of culture, hematopoietic cells were analyzed, by flow cytometry, for CD14 antigen expression, estimated at about 25-30% of the entire cell population. Then monoblasts (CD14+) and myeloblasts (CD14-) cell fractions were obtained by immunomagnetic separation using the MACS technology (Miltenyi). Differentiation of CD34+ cells was monitored by morphological analysis of MGG-stained cytospins and by flow-cytometric analysis of CD34, CD38 and CD14 surface antigen expression.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321583/suppl/GSM321583.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
| | |
|
GSM321584 | GPL96 |
|
Myeloblasts_2
|
In vitro differentiated normal human myeloblasts
|
type: in vitro differentiated cells derived from CD34+ stem/progenitor cells from healthy donors
|
In vitro differentiated normal human myeloblasts
|
| Sample_geo_accession | GSM321584
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Monoblasts (CD14+ precursors) and myeloblasts (CD14- precursors) were obtained by in vitro differentiation of cord blood (CB) derived CD34+ cells performed as already described by Montanari et al. Briefly, CB CD34+ cells were cultured in IMDM added with 20% FCS (Bio-Whittaker, Walkersville, MD, USA), in the presence of human hematopoietic cytokines: SCF (50 ng/ml), Flt3-ligand (Flt3-l) (50 ng/ml), IL-11 (50 ng/ml), IL-6 (10 ng/ml), IL-3 (10 ng/ml) and G-CSF (10 ng/ml) (all from R&D Systems, Minneapolis, MN, USA). After 7 days of culture, hematopoietic cells were analyzed, by flow cytometry, for CD14 antigen expression, estimated at about 25-30% of the entire cell population. Then monoblasts (CD14+) and myeloblasts (CD14-) cell fractions were obtained by immunomagnetic separation using the MACS technology (Miltenyi). Differentiation of CD34+ cells was monitored by morphological analysis of MGG-stained cytospins and by flow-cytometric analysis of CD34, CD38 and CD14 surface antigen expression.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321584/suppl/GSM321584.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
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GSM321585 | GPL96 |
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Myeloblasts_3
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In vitro differentiated normal human myeloblasts
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type: in vitro differentiated cells derived from CD34+ stem/progenitor cells from healthy donors
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In vitro differentiated normal human myeloblasts
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| Sample_geo_accession | GSM321585
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Monoblasts (CD14+ precursors) and myeloblasts (CD14- precursors) were obtained by in vitro differentiation of cord blood (CB) derived CD34+ cells performed as already described by Montanari et al. Briefly, CB CD34+ cells were cultured in IMDM added with 20% FCS (Bio-Whittaker, Walkersville, MD, USA), in the presence of human hematopoietic cytokines: SCF (50 ng/ml), Flt3-ligand (Flt3-l) (50 ng/ml), IL-11 (50 ng/ml), IL-6 (10 ng/ml), IL-3 (10 ng/ml) and G-CSF (10 ng/ml) (all from R&D Systems, Minneapolis, MN, USA). After 7 days of culture, hematopoietic cells were analyzed, by flow cytometry, for CD14 antigen expression, estimated at about 25-30% of the entire cell population. Then monoblasts (CD14+) and myeloblasts (CD14-) cell fractions were obtained by immunomagnetic separation using the MACS technology (Miltenyi). Differentiation of CD34+ cells was monitored by morphological analysis of MGG-stained cytospins and by flow-cytometric analysis of CD34, CD38 and CD14 surface antigen expression.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321585/suppl/GSM321585.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
| | |
|
GSM321586 | GPL96 |
|
Neutrophils_1
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Normal human neutrophils
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type: primary cells from healthy donors
|
Normal human neutrophils
|
| Sample_geo_accession | GSM321586
| | Sample_status | Public on Dec 10 2008
| | Sample_submission_date | Sep 16 2008
| | Sample_last_update_date | Sep 17 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Human granulocytes were initially collected from cell pellets obtained by Ficoll separation of peripheral blood samples. Erythrocytes contained in cell pellets were removed by means of osmotic lysis. Neutrophils (CD16+ fraction) and Eosinophils (CD16- fraction) were then purified using magnetic microbeads conjugated to mouse monoclonal anti-human CD16 Ab (Miltenyi, Auburn, CA)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
| | Sample_data_processing | The data were analyzed using Bioconductor packages and RMA pre-processing procedure
| | Sample_platform_id | GPL96
| | Sample_contact_name | Francesco,,Ferrari
| | Sample_contact_email | ferrari.francesco@unimore.it
| | Sample_contact_phone | +39(0)592055512
| | Sample_contact_fax | +39(0)592055410
| | Sample_contact_laboratory | Experimental genomics & transcriptomics laboratory
| | Sample_contact_department | Department of Biomedical Sciences
| | Sample_contact_institute | University of Modena and Reggio Emilia
| | Sample_contact_address | Via G. Campi 287
| | Sample_contact_city | Modena
| | Sample_contact_zip/postal_code | 41100
| | Sample_contact_country | Italy
| | Sample_contact_web_link | www.xlab.unimo.it
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM321nnn/GSM321586/suppl/GSM321586.CEL.gz
| | Sample_series_id | GSE12803
| | Sample_series_id | GSE12837
| | Sample_data_row_count | 22283
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