Search results for the GEO ID: GSE12843 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM322374 | GPL570 |
|
LM6, biological rep1
|
Human lipoma-derived mesenchymal stem cells
|
Gender: female
|
The mRNA profiles from LM6 cells and AM1cells were quantitatively determined.
|
Sample_geo_accession | GSM322374
| Sample_status | Public on Sep 08 2009
| Sample_submission_date | Sep 18 2008
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | LM6 cells and AM3 cells were cultured in the K-NAC medium that is a modified MCDB 153 medium (Keratinocyte-SFM, GIBCO-Invitrogen) supplemented with N-acetyl-L-cysteine (NAC; Sigma A8199) (2 mM) and L-ascorbic acid 2-phosphate (Asc 2P; sigma A8960) (0.2 mM).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNAs extracted using TRIZOL reagent were quantified spectrophotometrically. The cDNA synthesis was carried out by using SUPERSCRIPTTM one-step RT-PCR kit (Invitrogen). For the reverse transcription step, the whole 5 ul of the resuspended RNAs were incubated for 60 min at 42oC, then 15 min at 72oC in 50 ul of reaction mixture containing 25 ul of 2x Reaction Mix (Invitrogen) and 1 ul of RT/PLANTINUM Taq Mix (Invitrogen). 28.5 ul of the cDNAs present in the 50 ul RT reaction mixture purified by Microarray Target Purification Kit (Roche Applied Science, Indianapolis, ID, USA, http://www.roche-applies-science.com) were used as templates to amplify cDNA by Microarray Target Amplification Kit. The amplified cDNAs were purified by Microarray Target Purification Kit, and complementary RNAs (cRNAs) were synthesized from 200 ng cDNA with Microarray RNA Target Synthesis Kit (Roche Applied Science).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms purified cRNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45oC on Affymetrix Human Genome U133 plus 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips from the hybridization experiments were read by the Affymetrix GeneChip scanner 3000.
| Sample_data_processing | The data were processed by GeneSpring software version 7.2 GC-RMA preprocessor to use on the CEL files
| Sample_platform_id | GPL570
| Sample_contact_name | Sung-Liang,,Yu
| Sample_contact_email | slyu@ntu.edu.tw
| Sample_contact_phone | 886-2-23958341
| Sample_contact_fax | 886-2-23958341
| Sample_contact_laboratory | Microarray Core Facility
| Sample_contact_department | Clinical Laboratory Sciences and Medical Biotechnology
| Sample_contact_institute | National Taiwan University
| Sample_contact_address | Jen Ai Road Section1
| Sample_contact_city | Taipei
| Sample_contact_zip/postal_code | 100
| Sample_contact_country | Taiwan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM322nnn/GSM322374/suppl/GSM322374.CEL.gz
| Sample_relation | Reanalyzed by: GSE20125
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE12843
| Sample_data_row_count | 54675
| |
|
GSM322375 | GPL570 |
|
LM6, biological rep2
|
Human lipoma-derived mesenchymal stem cells
|
Gender: female
|
The mRNA profiles from LM6 cells and AM1cells were quantitatively determined.
|
Sample_geo_accession | GSM322375
| Sample_status | Public on Sep 08 2009
| Sample_submission_date | Sep 18 2008
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | LM6 cells and AM3 cells were cultured in the K-NAC medium that is a modified MCDB 153 medium (Keratinocyte-SFM, GIBCO-Invitrogen) supplemented with N-acetyl-L-cysteine (NAC; Sigma A8199) (2 mM) and L-ascorbic acid 2-phosphate (Asc 2P; sigma A8960) (0.2 mM).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNAs extracted using TRIZOL reagent were quantified spectrophotometrically. The cDNA synthesis was carried out by using SUPERSCRIPTTM one-step RT-PCR kit (Invitrogen). For the reverse transcription step, the whole 5 ul of the resuspended RNAs were incubated for 60 min at 42oC, then 15 min at 72oC in 50 ul of reaction mixture containing 25 ul of 2x Reaction Mix (Invitrogen) and 1 ul of RT/PLANTINUM Taq Mix (Invitrogen). 28.5 ul of the cDNAs present in the 50 ul RT reaction mixture purified by Microarray Target Purification Kit (Roche Applied Science, Indianapolis, ID, USA, http://www.roche-applies-science.com) were used as templates to amplify cDNA by Microarray Target Amplification Kit. The amplified cDNAs were purified by Microarray Target Purification Kit, and complementary RNAs (cRNAs) were synthesized from 200 ng cDNA with Microarray RNA Target Synthesis Kit (Roche Applied Science).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms purified cRNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45oC on Affymetrix Human Genome U133 plus 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips from the hybridization experiments were read by the Affymetrix GeneChip scanner 3000.
| Sample_data_processing | The data were processed by GeneSpring software version 7.2 GC-RMA preprocessor to use on the CEL files
| Sample_platform_id | GPL570
| Sample_contact_name | Sung-Liang,,Yu
| Sample_contact_email | slyu@ntu.edu.tw
| Sample_contact_phone | 886-2-23958341
| Sample_contact_fax | 886-2-23958341
| Sample_contact_laboratory | Microarray Core Facility
| Sample_contact_department | Clinical Laboratory Sciences and Medical Biotechnology
| Sample_contact_institute | National Taiwan University
| Sample_contact_address | Jen Ai Road Section1
| Sample_contact_city | Taipei
| Sample_contact_zip/postal_code | 100
| Sample_contact_country | Taiwan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM322nnn/GSM322375/suppl/GSM322375.CEL.gz
| Sample_relation | Reanalyzed by: GSE20125
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE12843
| Sample_data_row_count | 54675
| |
|
GSM322376 | GPL570 |
|
AM3, biological rep1
|
Human adipose-derived mesenchymal stem cells
|
Gender: female
|
The mRNA profiles from LM6 cells and AM1cells were quantitatively determined.
|
Sample_geo_accession | GSM322376
| Sample_status | Public on Sep 08 2009
| Sample_submission_date | Sep 18 2008
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | LM6 cells and AM3 cells were cultured in the K-NAC medium that is a modified MCDB 153 medium (Keratinocyte-SFM, GIBCO-Invitrogen) supplemented with N-acetyl-L-cysteine (NAC; Sigma A8199) (2 mM) and L-ascorbic acid 2-phosphate (Asc 2P; sigma A8960) (0.2 mM).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNAs extracted using TRIZOL reagent were quantified spectrophotometrically. The cDNA synthesis was carried out by using SUPERSCRIPTTM one-step RT-PCR kit (Invitrogen). For the reverse transcription step, the whole 5 ul of the resuspended RNAs were incubated for 60 min at 42oC, then 15 min at 72oC in 50 ul of reaction mixture containing 25 ul of 2x Reaction Mix (Invitrogen) and 1 ul of RT/PLANTINUM Taq Mix (Invitrogen). 28.5 ul of the cDNAs present in the 50 ul RT reaction mixture purified by Microarray Target Purification Kit (Roche Applied Science, Indianapolis, ID, USA, http://www.roche-applies-science.com) were used as templates to amplify cDNA by Microarray Target Amplification Kit. The amplified cDNAs were purified by Microarray Target Purification Kit, and complementary RNAs (cRNAs) were synthesized from 200 ng cDNA with Microarray RNA Target Synthesis Kit (Roche Applied Science).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms purified cRNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45oC on Affymetrix Human Genome U133 plus 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips from the hybridization experiments were read by the Affymetrix GeneChip scanner 3000.
| Sample_data_processing | The data were processed by GeneSpring software version 7.2 GC-RMA preprocessor to use on the CEL files
| Sample_platform_id | GPL570
| Sample_contact_name | Sung-Liang,,Yu
| Sample_contact_email | slyu@ntu.edu.tw
| Sample_contact_phone | 886-2-23958341
| Sample_contact_fax | 886-2-23958341
| Sample_contact_laboratory | Microarray Core Facility
| Sample_contact_department | Clinical Laboratory Sciences and Medical Biotechnology
| Sample_contact_institute | National Taiwan University
| Sample_contact_address | Jen Ai Road Section1
| Sample_contact_city | Taipei
| Sample_contact_zip/postal_code | 100
| Sample_contact_country | Taiwan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM322nnn/GSM322376/suppl/GSM322376.CEL.gz
| Sample_relation | Reanalyzed by: GSE20125
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE12843
| Sample_data_row_count | 54675
| |
|
GSM322377 | GPL570 |
|
AM3, biological rep2
|
Human adipose-derived mesenchymal stem cells
|
Gender: female
|
The mRNA profiles from LM6 cells and AM1cells were quantitatively determined.
|
Sample_geo_accession | GSM322377
| Sample_status | Public on Sep 08 2009
| Sample_submission_date | Sep 18 2008
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | LM6 cells and AM3 cells were cultured in the K-NAC medium that is a modified MCDB 153 medium (Keratinocyte-SFM, GIBCO-Invitrogen) supplemented with N-acetyl-L-cysteine (NAC; Sigma A8199) (2 mM) and L-ascorbic acid 2-phosphate (Asc 2P; sigma A8960) (0.2 mM).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNAs extracted using TRIZOL reagent were quantified spectrophotometrically. The cDNA synthesis was carried out by using SUPERSCRIPTTM one-step RT-PCR kit (Invitrogen). For the reverse transcription step, the whole 5 ul of the resuspended RNAs were incubated for 60 min at 42oC, then 15 min at 72oC in 50 ul of reaction mixture containing 25 ul of 2x Reaction Mix (Invitrogen) and 1 ul of RT/PLANTINUM Taq Mix (Invitrogen). 28.5 ul of the cDNAs present in the 50 ul RT reaction mixture purified by Microarray Target Purification Kit (Roche Applied Science, Indianapolis, ID, USA, http://www.roche-applies-science.com) were used as templates to amplify cDNA by Microarray Target Amplification Kit. The amplified cDNAs were purified by Microarray Target Purification Kit, and complementary RNAs (cRNAs) were synthesized from 200 ng cDNA with Microarray RNA Target Synthesis Kit (Roche Applied Science).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms purified cRNA (Expression Analysis Technical Manual, 2004, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45oC on Affymetrix Human Genome U133 plus 2.0 GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips from the hybridization experiments were read by the Affymetrix GeneChip scanner 3000.
| Sample_data_processing | The data were processed by GeneSpring software version 7.2 GC-RMA preprocessor to use on the CEL files
| Sample_platform_id | GPL570
| Sample_contact_name | Sung-Liang,,Yu
| Sample_contact_email | slyu@ntu.edu.tw
| Sample_contact_phone | 886-2-23958341
| Sample_contact_fax | 886-2-23958341
| Sample_contact_laboratory | Microarray Core Facility
| Sample_contact_department | Clinical Laboratory Sciences and Medical Biotechnology
| Sample_contact_institute | National Taiwan University
| Sample_contact_address | Jen Ai Road Section1
| Sample_contact_city | Taipei
| Sample_contact_zip/postal_code | 100
| Sample_contact_country | Taiwan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM322nnn/GSM322377/suppl/GSM322377.CEL.gz
| Sample_relation | Reanalyzed by: GSE20125
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE12843
| Sample_data_row_count | 54675
| |
|
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