Result

Search results for the GEO ID: GSE12854

(Click on the check boxes provided under "Select for analysis", to initiate grouping)

(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down)

GSM ID

GPL ID

Select for analysis

Title

Source name

Description

Characteristics

GSM322534

GPL570

OVCAR8 parental cells 1

OVCAR8 parental cells

OVCAR8

DCT-Tumor Repository (NCI)

Sample_geo_accession	GSM322534
Sample_status	Public on Sep 20 2008
Sample_submission_date	Sep 19 2008
Sample_last_update_date	Sep 19 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The stable transfectants, containing retroviral vectors OT1521 or OT1529 with the internal inducible mouse metallothionine-1 (MT-1) promoter, and genes encoding PKA subunits RIα and RIIβ were treated with 60 uM ZnSO4 for 6 days prior to the start of the experiment. To exclude other effects of Zn++, parental cells were treated with ZnSO4 in the same manner as transfected cells.
Sample_growth_protocol_ch1	OVCAR-8 cells were grown in RPMI Medium 1640 supplemented with 10% heat-inactivated fetal bovine serum, MEM non-essential amino acids, and antibiotic-antimycotic, in a humidified incubator (95% air and 5% CO2) at 37⁰C.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. Additional purification was performed on RNAeasy columns.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with the Affymetrix Expression Console Software using the Robust Multichip Analysis (RMA) default analysis settings as the normalization method.
Sample_platform_id	GPL570
Sample_contact_name	chris,,cheadle
Sample_contact_email	ccheadl1@jhmi.edu
Sample_contact_phone	4105505984
Sample_contact_laboratory	JHBMC Genomics Core
Sample_contact_department	SOM
Sample_contact_institute	JHU
Sample_contact_address	5200 Eastern Ave
Sample_contact_city	baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21224
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM322nnn/GSM322534/suppl/GSM322534.CEL.gz
Sample_series_id	GSE12854
Sample_data_row_count	54675

GSM322535

GPL570

OVCAR8 parental cells 2

OVCAR8 parental cells

OVCAR8

DCT-Tumor Repository (NCI)

Sample_geo_accession	GSM322535
Sample_status	Public on Sep 20 2008
Sample_submission_date	Sep 19 2008
Sample_last_update_date	Sep 19 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The stable transfectants, containing retroviral vectors OT1521 or OT1529 with the internal inducible mouse metallothionine-1 (MT-1) promoter, and genes encoding PKA subunits RIα and RIIβ were treated with 60 uM ZnSO4 for 6 days prior to the start of the experiment. To exclude other effects of Zn++, parental cells were treated with ZnSO4 in the same manner as transfected cells.
Sample_growth_protocol_ch1	OVCAR-8 cells were grown in RPMI Medium 1640 supplemented with 10% heat-inactivated fetal bovine serum, MEM non-essential amino acids, and antibiotic-antimycotic, in a humidified incubator (95% air and 5% CO2) at 37⁰C.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. Additional purification was performed on RNAeasy columns.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with the Affymetrix Expression Console Software using the Robust Multichip Analysis (RMA) default analysis settings as the normalization method.
Sample_platform_id	GPL570
Sample_contact_name	chris,,cheadle
Sample_contact_email	ccheadl1@jhmi.edu
Sample_contact_phone	4105505984
Sample_contact_laboratory	JHBMC Genomics Core
Sample_contact_department	SOM
Sample_contact_institute	JHU
Sample_contact_address	5200 Eastern Ave
Sample_contact_city	baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21224
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM322nnn/GSM322535/suppl/GSM322535.CEL.gz
Sample_series_id	GSE12854
Sample_data_row_count	54675

GSM322536

GPL570

OVCAR8 parental cells 3

OVCAR8 parental cells

OVCAR8

DCT-Tumor Repository (NCI)

Sample_geo_accession	GSM322536
Sample_status	Public on Sep 20 2008
Sample_submission_date	Sep 19 2008
Sample_last_update_date	Sep 19 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The stable transfectants, containing retroviral vectors OT1521 or OT1529 with the internal inducible mouse metallothionine-1 (MT-1) promoter, and genes encoding PKA subunits RIα and RIIβ were treated with 60 uM ZnSO4 for 6 days prior to the start of the experiment. To exclude other effects of Zn++, parental cells were treated with ZnSO4 in the same manner as transfected cells.
Sample_growth_protocol_ch1	OVCAR-8 cells were grown in RPMI Medium 1640 supplemented with 10% heat-inactivated fetal bovine serum, MEM non-essential amino acids, and antibiotic-antimycotic, in a humidified incubator (95% air and 5% CO2) at 37⁰C.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. Additional purification was performed on RNAeasy columns.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with the Affymetrix Expression Console Software using the Robust Multichip Analysis (RMA) default analysis settings as the normalization method.
Sample_platform_id	GPL570
Sample_contact_name	chris,,cheadle
Sample_contact_email	ccheadl1@jhmi.edu
Sample_contact_phone	4105505984
Sample_contact_laboratory	JHBMC Genomics Core
Sample_contact_department	SOM
Sample_contact_institute	JHU
Sample_contact_address	5200 Eastern Ave
Sample_contact_city	baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21224
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM322nnn/GSM322536/suppl/GSM322536.CEL.gz
Sample_series_id	GSE12854
Sample_data_row_count	54675

GSM322537

GPL570

OVCAR8 RIA transfectant 1

OVCAR8 RIA transfectant

OVCAR8

DCT-Tumor Repository (NCI)

Sample_geo_accession	GSM322537
Sample_status	Public on Sep 20 2008
Sample_submission_date	Sep 19 2008
Sample_last_update_date	Sep 19 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The stable transfectants, containing retroviral vectors OT1521 or OT1529 with the internal inducible mouse metallothionine-1 (MT-1) promoter, and genes encoding PKA subunits RIα and RIIβ were treated with 60 uM ZnSO4 for 6 days prior to the start of the experiment. To exclude other effects of Zn++, parental cells were treated with ZnSO4 in the same manner as transfected cells.
Sample_growth_protocol_ch1	OVCAR-8 cells were grown in RPMI Medium 1640 supplemented with 10% heat-inactivated fetal bovine serum, MEM non-essential amino acids, and antibiotic-antimycotic, in a humidified incubator (95% air and 5% CO2) at 37⁰C.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. Additional purification was performed on RNAeasy columns.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with the Affymetrix Expression Console Software using the Robust Multichip Analysis (RMA) default analysis settings as the normalization method.
Sample_platform_id	GPL570
Sample_contact_name	chris,,cheadle
Sample_contact_email	ccheadl1@jhmi.edu
Sample_contact_phone	4105505984
Sample_contact_laboratory	JHBMC Genomics Core
Sample_contact_department	SOM
Sample_contact_institute	JHU
Sample_contact_address	5200 Eastern Ave
Sample_contact_city	baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21224
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM322nnn/GSM322537/suppl/GSM322537.CEL.gz
Sample_series_id	GSE12854
Sample_data_row_count	54675

GSM322538

GPL570

OVCAR8 RIA transfectant 2

OVCAR8 RIA transfectant

OVCAR8

DCT-Tumor Repository (NCI)

Sample_geo_accession	GSM322538
Sample_status	Public on Sep 20 2008
Sample_submission_date	Sep 19 2008
Sample_last_update_date	Sep 19 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The stable transfectants, containing retroviral vectors OT1521 or OT1529 with the internal inducible mouse metallothionine-1 (MT-1) promoter, and genes encoding PKA subunits RIα and RIIβ were treated with 60 uM ZnSO4 for 6 days prior to the start of the experiment. To exclude other effects of Zn++, parental cells were treated with ZnSO4 in the same manner as transfected cells.
Sample_growth_protocol_ch1	OVCAR-8 cells were grown in RPMI Medium 1640 supplemented with 10% heat-inactivated fetal bovine serum, MEM non-essential amino acids, and antibiotic-antimycotic, in a humidified incubator (95% air and 5% CO2) at 37⁰C.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. Additional purification was performed on RNAeasy columns.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with the Affymetrix Expression Console Software using the Robust Multichip Analysis (RMA) default analysis settings as the normalization method.
Sample_platform_id	GPL570
Sample_contact_name	chris,,cheadle
Sample_contact_email	ccheadl1@jhmi.edu
Sample_contact_phone	4105505984
Sample_contact_laboratory	JHBMC Genomics Core
Sample_contact_department	SOM
Sample_contact_institute	JHU
Sample_contact_address	5200 Eastern Ave
Sample_contact_city	baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21224
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM322nnn/GSM322538/suppl/GSM322538.CEL.gz
Sample_series_id	GSE12854
Sample_data_row_count	54675

GSM322539

GPL570

OVCAR8 RIA transfectant 3

OVCAR8 RIA transfectant

OVCAR8

DCT-Tumor Repository (NCI)

Sample_geo_accession	GSM322539
Sample_status	Public on Sep 20 2008
Sample_submission_date	Sep 19 2008
Sample_last_update_date	Sep 19 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The stable transfectants, containing retroviral vectors OT1521 or OT1529 with the internal inducible mouse metallothionine-1 (MT-1) promoter, and genes encoding PKA subunits RIα and RIIβ were treated with 60 uM ZnSO4 for 6 days prior to the start of the experiment. To exclude other effects of Zn++, parental cells were treated with ZnSO4 in the same manner as transfected cells.
Sample_growth_protocol_ch1	OVCAR-8 cells were grown in RPMI Medium 1640 supplemented with 10% heat-inactivated fetal bovine serum, MEM non-essential amino acids, and antibiotic-antimycotic, in a humidified incubator (95% air and 5% CO2) at 37⁰C.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. Additional purification was performed on RNAeasy columns.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with the Affymetrix Expression Console Software using the Robust Multichip Analysis (RMA) default analysis settings as the normalization method.
Sample_platform_id	GPL570
Sample_contact_name	chris,,cheadle
Sample_contact_email	ccheadl1@jhmi.edu
Sample_contact_phone	4105505984
Sample_contact_laboratory	JHBMC Genomics Core
Sample_contact_department	SOM
Sample_contact_institute	JHU
Sample_contact_address	5200 Eastern Ave
Sample_contact_city	baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21224
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM322nnn/GSM322539/suppl/GSM322539.CEL.gz
Sample_series_id	GSE12854
Sample_data_row_count	54675

GSM322540

GPL570

OVCAR8 RIIB transfectant 1

OVCAR8 RIIB transfectant

OVCAR8

DCT-Tumor Repository (NCI)

Sample_geo_accession	GSM322540
Sample_status	Public on Sep 20 2008
Sample_submission_date	Sep 19 2008
Sample_last_update_date	Sep 19 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The stable transfectants, containing retroviral vectors OT1521 or OT1529 with the internal inducible mouse metallothionine-1 (MT-1) promoter, and genes encoding PKA subunits RIα and RIIβ were treated with 60 uM ZnSO4 for 6 days prior to the start of the experiment. To exclude other effects of Zn++, parental cells were treated with ZnSO4 in the same manner as transfected cells.
Sample_growth_protocol_ch1	OVCAR-8 cells were grown in RPMI Medium 1640 supplemented with 10% heat-inactivated fetal bovine serum, MEM non-essential amino acids, and antibiotic-antimycotic, in a humidified incubator (95% air and 5% CO2) at 37⁰C.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. Additional purification was performed on RNAeasy columns.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with the Affymetrix Expression Console Software using the Robust Multichip Analysis (RMA) default analysis settings as the normalization method.
Sample_platform_id	GPL570
Sample_contact_name	chris,,cheadle
Sample_contact_email	ccheadl1@jhmi.edu
Sample_contact_phone	4105505984
Sample_contact_laboratory	JHBMC Genomics Core
Sample_contact_department	SOM
Sample_contact_institute	JHU
Sample_contact_address	5200 Eastern Ave
Sample_contact_city	baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21224
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM322nnn/GSM322540/suppl/GSM322540.CEL.gz
Sample_series_id	GSE12854
Sample_data_row_count	54675

GSM322541

GPL570

OVCAR8 RIIB transfectant 2

OVCAR8 RIIB transfectant

OVCAR8

DCT-Tumor Repository (NCI)

Sample_geo_accession	GSM322541
Sample_status	Public on Sep 20 2008
Sample_submission_date	Sep 19 2008
Sample_last_update_date	Sep 19 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The stable transfectants, containing retroviral vectors OT1521 or OT1529 with the internal inducible mouse metallothionine-1 (MT-1) promoter, and genes encoding PKA subunits RIα and RIIβ were treated with 60 uM ZnSO4 for 6 days prior to the start of the experiment. To exclude other effects of Zn++, parental cells were treated with ZnSO4 in the same manner as transfected cells.
Sample_growth_protocol_ch1	OVCAR-8 cells were grown in RPMI Medium 1640 supplemented with 10% heat-inactivated fetal bovine serum, MEM non-essential amino acids, and antibiotic-antimycotic, in a humidified incubator (95% air and 5% CO2) at 37⁰C.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. Additional purification was performed on RNAeasy columns.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with the Affymetrix Expression Console Software using the Robust Multichip Analysis (RMA) default analysis settings as the normalization method.
Sample_platform_id	GPL570
Sample_contact_name	chris,,cheadle
Sample_contact_email	ccheadl1@jhmi.edu
Sample_contact_phone	4105505984
Sample_contact_laboratory	JHBMC Genomics Core
Sample_contact_department	SOM
Sample_contact_institute	JHU
Sample_contact_address	5200 Eastern Ave
Sample_contact_city	baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21224
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM322nnn/GSM322541/suppl/GSM322541.CEL.gz
Sample_series_id	GSE12854
Sample_data_row_count	54675

GSM322542

GPL570

OVCAR8 RIIB transfectant 3

OVCAR8 RIIB transfectant

OVCAR8

DCT-Tumor Repository (NCI)

Sample_geo_accession	GSM322542
Sample_status	Public on Sep 20 2008
Sample_submission_date	Sep 19 2008
Sample_last_update_date	Sep 19 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	The stable transfectants, containing retroviral vectors OT1521 or OT1529 with the internal inducible mouse metallothionine-1 (MT-1) promoter, and genes encoding PKA subunits RIα and RIIβ were treated with 60 uM ZnSO4 for 6 days prior to the start of the experiment. To exclude other effects of Zn++, parental cells were treated with ZnSO4 in the same manner as transfected cells.
Sample_growth_protocol_ch1	OVCAR-8 cells were grown in RPMI Medium 1640 supplemented with 10% heat-inactivated fetal bovine serum, MEM non-essential amino acids, and antibiotic-antimycotic, in a humidified incubator (95% air and 5% CO2) at 37⁰C.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions. Additional purification was performed on RNAeasy columns.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneArray Scanner G2500A.
Sample_data_processing	The data were analyzed with the Affymetrix Expression Console Software using the Robust Multichip Analysis (RMA) default analysis settings as the normalization method.
Sample_platform_id	GPL570
Sample_contact_name	chris,,cheadle
Sample_contact_email	ccheadl1@jhmi.edu
Sample_contact_phone	4105505984
Sample_contact_laboratory	JHBMC Genomics Core
Sample_contact_department	SOM
Sample_contact_institute	JHU
Sample_contact_address	5200 Eastern Ave
Sample_contact_city	baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21224
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM322nnn/GSM322542/suppl/GSM322542.CEL.gz
Sample_series_id	GSE12854
Sample_data_row_count	54675

Make groups for comparisons

(2 groups will be compared at a time)

Select GSMs and click on "Add groups"

Enter the group name here:

Select expression type

Transcripts profile based on;

A. Differential status (Up/Down regulation)

B. Absolute calls (Transcribed/Not-detected)

Filter results by number of probes