Search results for the GEO ID: GSE12891  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM323168 | GPL570 |
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human umbilical cord blood derived endothelial progenitor cell
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endothelial progenitor cell
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human umbilical cord blood derived endothelial progenitor cell
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human umbilical cord blood derived endothelial progenitor cell
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| Sample_geo_accession | GSM323168
| | Sample_status | Public on Sep 24 2008
| | Sample_submission_date | Sep 23 2008
| | Sample_last_update_date | Sep 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | EPCs from human umbilical cord blood (HUCB) were cultured on fibronectin coated plate for 7 days . RNA was extracted using the Invisorb RNA Kit II (Invitek, Berlin, Germany) according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
| | Sample_extract_protocol_ch1 |
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the BioArray-High-Yield-RNA-Transcript-Labeling-Kit (Enzo, Farmingdale, NY).
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized to the Affymetrix HG-U133A chip according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 7GPLUS
| | Sample_data_processing | Expression data were analyzed using Microarray Suite version 5.0 (Affymetrix) and GenPlex v2.4 software (ISTECH Inc. Korea).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Young-Guen,,Kwon
| | Sample_contact_email | ygkwon@yonsei.ac.kr
| | Sample_contact_department | Dppartment of Biochemistry
| | Sample_contact_institute | Yonsei University
| | Sample_contact_address | 262 Seongsanno, Seodaemun-Gu
| | Sample_contact_city | Seoul
| | Sample_contact_zip/postal_code | 120-749
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM323nnn/GSM323168/suppl/GSM323168.CEL.gz
| | Sample_series_id | GSE12891
| | Sample_data_row_count | 54675
| | |
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GSM323169 | GPL570 |
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Outgrowth endothelial cell differentiated from endothelial progenitor cell
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Outgrowth endothelial cell
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Outgrowth endothelial cell differentiated from human umbilical cord blood derived-endothelial progenitor cell
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Outgrowth endothelial cell differentiated from human umbilical cord blood derived-endothelial progenitor cell
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| Sample_geo_accession | GSM323169
| | Sample_status | Public on Sep 24 2008
| | Sample_submission_date | Sep 23 2008
| | Sample_last_update_date | Sep 23 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | OEC naturally differentiated from human umbilical cord blood-derived EPCs. RNA was extracted using the Invisorb RNA Kit II (Invitek, Berlin, Germany) according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
| | Sample_extract_protocol_ch1 |
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 2 microg total RNA using the BioArray-High-Yield-RNA-Transcript-Labeling-Kit (Enzo, Farmingdale, NY).
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized to the Affymetrix HG-U133A chip according to the protocols provided by the manufacturer.
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 7GPLUS
| | Sample_data_processing | Expression data were analyzed using Microarray Suite version 5.0 (Affymetrix) and GenPlex v2.4 software (ISTECH Inc. Korea).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Young-Guen,,Kwon
| | Sample_contact_email | ygkwon@yonsei.ac.kr
| | Sample_contact_department | Dppartment of Biochemistry
| | Sample_contact_institute | Yonsei University
| | Sample_contact_address | 262 Seongsanno, Seodaemun-Gu
| | Sample_contact_city | Seoul
| | Sample_contact_zip/postal_code | 120-749
| | Sample_contact_country | South Korea
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM323nnn/GSM323169/suppl/GSM323169.CEL.gz
| | Sample_series_id | GSE12891
| | Sample_data_row_count | 54675
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