Result

Search results for the GEO ID: GSE12993

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM325533

GPL1261

C2C12-GM

mouse C2C12 culture in Growth Medium

C2C12

Gene expression data from C2C12 culture in GM, i.e. before change to DM.

Sample_geo_accession	GSM325533
Sample_status	Public on Jul 31 2009
Sample_submission_date	Sep 30 2008
Sample_last_update_date	Jun 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	C2C12 murine skeletal muscle cells were purchased from American Type culture Collection (ATCC). These cells were maintained in GM (DMEM supplemented with 10% FBS). Cells were grown in GM and after reaching confluence, the medium was switched to DM (DMEM supplemented with 2% hourse serum) and further incubated. The medium was changed every 2 days. Culture was performed by using within five passages cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA of the culture was extracted using ISOGEN (NIPPONGENE).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from total RNA following standard Affymetrix® protocols
Sample_hyb_protocol	Hybridization to Affymetrix® GeneChip® Mouse Genome 430 2.0 arrays was carried out following standard Affymetrix® protocols. 10 ug cRNA used.
Sample_scan_protocol	GeneChips® were scanned using GeneChip Scanner 3000
Sample_data_processing	Affymetrix® microarray image data were collected with Affymetrix® GeneChip® Operating Software version 1.1 using default parameters. We used the Bioconductor 'affy' software package for further data analysis. Signal intensities were calculated with the 'rma' function using default parameters, including quantile normalization and log2 transformation.
Sample_platform_id	GPL1261
Sample_contact_name	Hiroshi,,Asahara
Sample_contact_email	asahara@nch.go.jp
Sample_contact_phone	+81-3-3417-2948
Sample_contact_fax	+81-3-3417-2498
Sample_contact_department	Department of Systems BioMedicine
Sample_contact_institute	National Research Institute for Child Health and Development
Sample_contact_address	2-10-1 Okura
Sample_contact_city	Setagaya
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	157-8535
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM325nnn/GSM325533/suppl/GSM325533.CEL.gz
Sample_series_id	GSE12993
Sample_data_row_count	45101

GSM325534

GPL1261

C2C12-day0

mouse C2C12 culture at Differentiation day0

C2C12

Gene expression data from C2C12 culture at day0, i.e. just after change to DM.

Sample_geo_accession	GSM325534
Sample_status	Public on Jul 31 2009
Sample_submission_date	Sep 30 2008
Sample_last_update_date	Jun 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	C2C12 murine skeletal muscle cells were purchased from American Type culture Collection (ATCC). These cells were maintained in GM (DMEM supplemented with 10% FBS). Cells were grown in GM and after reaching confluence, the medium was switched to DM (DMEM supplemented with 2% hourse serum) and further incubated. The medium was changed every 2 days. Culture was performed by using within five passages cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA of the culture was extracted using ISOGEN (NIPPONGENE).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from total RNA following standard Affymetrix® protocols
Sample_hyb_protocol	Hybridization to Affymetrix® GeneChip® Mouse Genome 430 2.0 arrays was carried out following standard Affymetrix® protocols. 10 ug cRNA used.
Sample_scan_protocol	GeneChips® were scanned using GeneChip Scanner 3000
Sample_data_processing	Affymetrix® microarray image data were collected with Affymetrix® GeneChip® Operating Software version 1.1 using default parameters. We used the Bioconductor 'affy' software package for further data analysis. Signal intensities were calculated with the 'rma' function using default parameters, including quantile normalization and log2 transformation.
Sample_platform_id	GPL1261
Sample_contact_name	Hiroshi,,Asahara
Sample_contact_email	asahara@nch.go.jp
Sample_contact_phone	+81-3-3417-2948
Sample_contact_fax	+81-3-3417-2498
Sample_contact_department	Department of Systems BioMedicine
Sample_contact_institute	National Research Institute for Child Health and Development
Sample_contact_address	2-10-1 Okura
Sample_contact_city	Setagaya
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	157-8535
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM325nnn/GSM325534/suppl/GSM325534.CEL.gz
Sample_series_id	GSE12993
Sample_data_row_count	45101

GSM325535

GPL1261

C2C12-day2

mouse C2C12 culture at Differentiation day2

C2C12

Gene expression data from C2C12 culture at day2, i.e. 2 day after change to DM.

Sample_geo_accession	GSM325535
Sample_status	Public on Jul 31 2009
Sample_submission_date	Sep 30 2008
Sample_last_update_date	Jun 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	C2C12 murine skeletal muscle cells were purchased from American Type culture Collection (ATCC). These cells were maintained in GM (DMEM supplemented with 10% FBS). Cells were grown in GM and after reaching confluence, the medium was switched to DM (DMEM supplemented with 2% hourse serum) and further incubated. The medium was changed every 2 days. Culture was performed by using within five passages cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA of the culture was extracted using ISOGEN (NIPPONGENE).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from total RNA following standard Affymetrix® protocols
Sample_hyb_protocol	Hybridization to Affymetrix® GeneChip® Mouse Genome 430 2.0 arrays was carried out following standard Affymetrix® protocols. 10 ug cRNA used.
Sample_scan_protocol	GeneChips® were scanned using GeneChip Scanner 3000
Sample_data_processing	Affymetrix® microarray image data were collected with Affymetrix® GeneChip® Operating Software version 1.1 using default parameters. We used the Bioconductor 'affy' software package for further data analysis. Signal intensities were calculated with the 'rma' function using default parameters, including quantile normalization and log2 transformation.
Sample_platform_id	GPL1261
Sample_contact_name	Hiroshi,,Asahara
Sample_contact_email	asahara@nch.go.jp
Sample_contact_phone	+81-3-3417-2948
Sample_contact_fax	+81-3-3417-2498
Sample_contact_department	Department of Systems BioMedicine
Sample_contact_institute	National Research Institute for Child Health and Development
Sample_contact_address	2-10-1 Okura
Sample_contact_city	Setagaya
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	157-8535
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM325nnn/GSM325535/suppl/GSM325535.CEL.gz
Sample_series_id	GSE12993
Sample_data_row_count	45101

GSM325536

GPL1261

C2C12-day4

mouse C2C12 culture at Differentiation day4

C2C12

Gene expression data from C2C12 culture at day4, i.e. 4 day after change to DM.

Sample_geo_accession	GSM325536
Sample_status	Public on Jul 31 2009
Sample_submission_date	Sep 30 2008
Sample_last_update_date	Jun 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	C2C12 murine skeletal muscle cells were purchased from American Type culture Collection (ATCC). These cells were maintained in GM (DMEM supplemented with 10% FBS). Cells were grown in GM and after reaching confluence, the medium was switched to DM (DMEM supplemented with 2% hourse serum) and further incubated. The medium was changed every 2 days. Culture was performed by using within five passages cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA of the culture was extracted using ISOGEN (NIPPONGENE).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from total RNA following standard Affymetrix® protocols
Sample_hyb_protocol	Hybridization to Affymetrix® GeneChip® Mouse Genome 430 2.0 arrays was carried out following standard Affymetrix® protocols. 10 ug cRNA used.
Sample_scan_protocol	GeneChips® were scanned using GeneChip Scanner 3000
Sample_data_processing	Affymetrix® microarray image data were collected with Affymetrix® GeneChip® Operating Software version 1.1 using default parameters. We used the Bioconductor 'affy' software package for further data analysis. Signal intensities were calculated with the 'rma' function using default parameters, including quantile normalization and log2 transformation.
Sample_platform_id	GPL1261
Sample_contact_name	Hiroshi,,Asahara
Sample_contact_email	asahara@nch.go.jp
Sample_contact_phone	+81-3-3417-2948
Sample_contact_fax	+81-3-3417-2498
Sample_contact_department	Department of Systems BioMedicine
Sample_contact_institute	National Research Institute for Child Health and Development
Sample_contact_address	2-10-1 Okura
Sample_contact_city	Setagaya
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	157-8535
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM325nnn/GSM325536/suppl/GSM325536.CEL.gz
Sample_series_id	GSE12993
Sample_data_row_count	45101

GSM325537

GPL1261

C2C12-control-day0

mouse C2C12 culture treated control LacZ vector at day0

C2C12

Gene expression data from C2C12 culture treated stable control sh at day0

Sample_geo_accession	GSM325537
Sample_status	Public on Jul 31 2009
Sample_submission_date	Sep 30 2008
Sample_last_update_date	Jun 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the experiment of shRNA for Rp58, transfection was performed by using Lipofectamin 2000 (Invitrogen). Stable transfectants were obtained by selection of the transfected C2C12 cells by G418 (500 μg/ml, GIBCO) for two weeks.
Sample_growth_protocol_ch1	C2C12 murine skeletal muscle cells were purchased from American Type culture Collection (ATCC). These cells were maintained in GM (DMEM supplemented with 10% FBS). Cells were grown in GM and after reaching confluence, the medium was switched to DM (DMEM supplemented with 2% hourse serum) and further incubated. The medium was changed every 2 days. Culture was performed by using within five passages cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA of the culture was extracted using ISOGEN (NIPPONGENE).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from total RNA following standard Affymetrix® protocols
Sample_hyb_protocol	Hybridization to Affymetrix® GeneChip® Mouse Genome 430 2.0 arrays was carried out following standard Affymetrix® protocols. 10 ug cRNA used.
Sample_scan_protocol	GeneChips® were scanned using GeneChip Scanner 3000
Sample_data_processing	Affymetrix® microarray image data were collected with Affymetrix® GeneChip® Operating Software version 1.1 using default parameters. We used the Bioconductor 'affy' software package for further data analysis. Signal intensities were calculated with the 'rma' function using default parameters, including quantile normalization and log2 transformation.
Sample_platform_id	GPL1261
Sample_contact_name	Hiroshi,,Asahara
Sample_contact_email	asahara@nch.go.jp
Sample_contact_phone	+81-3-3417-2948
Sample_contact_fax	+81-3-3417-2498
Sample_contact_department	Department of Systems BioMedicine
Sample_contact_institute	National Research Institute for Child Health and Development
Sample_contact_address	2-10-1 Okura
Sample_contact_city	Setagaya
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	157-8535
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM325nnn/GSM325537/suppl/GSM325537.CEL.gz
Sample_series_id	GSE12993
Sample_data_row_count	45101

GSM325538

GPL1261

C2C12-shRp58-day0

mouse C2C12 culture treated shRp58 vector at day0

C2C12

Gene expression data from C2C12 culture treated stable shRp58 at day0

Sample_geo_accession	GSM325538
Sample_status	Public on Jul 31 2009
Sample_submission_date	Sep 30 2008
Sample_last_update_date	Jun 22 2009
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the experiment of shRNA for Rp58, transfection was performed by using Lipofectamin 2000 (Invitrogen). Stable transfectants were obtained by selection of the transfected C2C12 cells by G418 (500 μg/ml, GIBCO) for two weeks.
Sample_growth_protocol_ch1	C2C12 murine skeletal muscle cells were purchased from American Type culture Collection (ATCC). These cells were maintained in GM (DMEM supplemented with 10% FBS). Cells were grown in GM and after reaching confluence, the medium was switched to DM (DMEM supplemented with 2% hourse serum) and further incubated. The medium was changed every 2 days. Culture was performed by using within five passages cells.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA of the culture was extracted using ISOGEN (NIPPONGENE).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from total RNA following standard Affymetrix® protocols
Sample_hyb_protocol	Hybridization to Affymetrix® GeneChip® Mouse Genome 430 2.0 arrays was carried out following standard Affymetrix® protocols. 10 ug cRNA used.
Sample_scan_protocol	GeneChips® were scanned using GeneChip Scanner 3000
Sample_data_processing	Affymetrix® microarray image data were collected with Affymetrix® GeneChip® Operating Software version 1.1 using default parameters. We used the Bioconductor 'affy' software package for further data analysis. Signal intensities were calculated with the 'rma' function using default parameters, including quantile normalization and log2 transformation.
Sample_platform_id	GPL1261
Sample_contact_name	Hiroshi,,Asahara
Sample_contact_email	asahara@nch.go.jp
Sample_contact_phone	+81-3-3417-2948
Sample_contact_fax	+81-3-3417-2498
Sample_contact_department	Department of Systems BioMedicine
Sample_contact_institute	National Research Institute for Child Health and Development
Sample_contact_address	2-10-1 Okura
Sample_contact_city	Setagaya
Sample_contact_state	Tokyo
Sample_contact_zip/postal_code	157-8535
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM325nnn/GSM325538/suppl/GSM325538.CEL.gz
Sample_series_id	GSE12993
Sample_data_row_count	45101

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