Result

Search results for the GEO ID: GSE13103

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Title

Source name

Description

Characteristics

GSM327854

GPL1261

Optic fissure_mouse_E10.5_A

Mouse embryo, optic fissure tissue, embryonic day 10.5

Strain: C57BL/6, Age: Embryonic day 10.5, Tissue: Optic fissure

Expression data were gathered in biological triplicate at E10.5, E11.5 and duplicate at E12.5, each array representing pooled optic fissure tissue from three embryos from a single litter.

Sample_geo_accession	GSM327854
Sample_status	Public on Oct 08 2008
Sample_submission_date	Oct 07 2008
Sample_last_update_date	Oct 07 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Wild type embryos dissected in Rnase free 1X PBS.
Sample_growth_protocol_ch1	A Veritas laser capture microdissection instrument (Molecular Devices) was used to collect optic fissure tissue from frozen sections through staged, wild-type C57BL6/J mouse embryos.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Tissue lysates from three embryos from separate litters and the same embryonic stage were pooled and total RNA was extracted using Absolutely RNA Microprep Kit (Stratagene). Pooled RNA was amplified linearly with two rounds of T7 polymerase-mediated in vitro transcription as per instructions in RiboAmp OA kit (Molecular Devices).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin labeling of transcripts was performed in the final round of in vitro transcription using GeneChip IVT Labeling Kit (Affymetrix).
Sample_hyb_protocol	Labeled samples from each timepoint were then hybridized to Affymetrix MOE 430 2.0 expression microarrays according to manufacturer’s protocol and scanned using GeneChip Scanner 3000 (Affymetrix).
Sample_scan_protocol	Labeled samples from each timepoint were then hybridized to Affymetrix MOE 430 2.0 expression microarrays according to manufacturer’s protocol and scanned using GeneChip Scanner 3000 (Affymetrix).
Sample_data_processing	Raw expression signals and present/absent calls were made using Affymetrix GCOS 1.4.
Sample_platform_id	GPL1261
Sample_contact_name	Jacob,,Brown
Sample_contact_institute	NEI
Sample_contact_address	10 Center Drive
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20816
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM327nnn/GSM327854/suppl/GSM327854.CEL.gz
Sample_series_id	GSE13103
Sample_data_row_count	45101

GSM327855

GPL1261

Optic fissure_mouse_E10.5_B

Mouse embryo, optic fissure tissue, embryonic day 10.5

Strain: C57BL/6, Age: Embryonic day 10.5, Tissue: Optic fissure

Expression data were gathered in biological triplicate at E10.5, E11.5 and duplicate at E12.5, each array representing pooled optic fissure tissue from three embryos from a single litter.

Sample_geo_accession	GSM327855
Sample_status	Public on Oct 08 2008
Sample_submission_date	Oct 07 2008
Sample_last_update_date	Oct 07 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Wild type embryos dissected in Rnase free 1X PBS.
Sample_growth_protocol_ch1	A Veritas laser capture microdissection instrument (Molecular Devices) was used to collect optic fissure tissue from frozen sections through staged, wild-type C57BL6/J mouse embryos.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Tissue lysates from three embryos from separate litters and the same embryonic stage were pooled and total RNA was extracted using Absolutely RNA Microprep Kit (Stratagene). Pooled RNA was amplified linearly with two rounds of T7 polymerase-mediated in vitro transcription as per instructions in RiboAmp OA kit (Molecular Devices).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin labeling of transcripts was performed in the final round of in vitro transcription using GeneChip IVT Labeling Kit (Affymetrix).
Sample_hyb_protocol	Labeled samples from each timepoint were then hybridized to Affymetrix MOE 430 2.0 expression microarrays according to manufacturer’s protocol and scanned using GeneChip Scanner 3000 (Affymetrix).
Sample_scan_protocol	Labeled samples from each timepoint were then hybridized to Affymetrix MOE 430 2.0 expression microarrays according to manufacturer’s protocol and scanned using GeneChip Scanner 3000 (Affymetrix).
Sample_data_processing	Raw expression signals and present/absent calls were made using Affymetrix GCOS 1.4.
Sample_platform_id	GPL1261
Sample_contact_name	Jacob,,Brown
Sample_contact_institute	NEI
Sample_contact_address	10 Center Drive
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20816
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM327nnn/GSM327855/suppl/GSM327855.CEL.gz
Sample_series_id	GSE13103
Sample_data_row_count	45101

GSM327856

GPL1261

Optic fissure_mouse_E10.5_C

Mouse embryo, optic fissure tissue, embryonic day 10.5

Strain: C57BL/6, Age: Embryonic day 10.5, Tissue: Optic fissure

Expression data were gathered in biological triplicate at E10.5, E11.5 and duplicate at E12.5, each array representing pooled optic fissure tissue from three embryos from a single litter.

Sample_geo_accession	GSM327856
Sample_status	Public on Oct 08 2008
Sample_submission_date	Oct 07 2008
Sample_last_update_date	Oct 07 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Wild type embryos dissected in Rnase free 1X PBS.
Sample_growth_protocol_ch1	A Veritas laser capture microdissection instrument (Molecular Devices) was used to collect optic fissure tissue from frozen sections through staged, wild-type C57BL6/J mouse embryos.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Tissue lysates from three embryos from separate litters and the same embryonic stage were pooled and total RNA was extracted using Absolutely RNA Microprep Kit (Stratagene). Pooled RNA was amplified linearly with two rounds of T7 polymerase-mediated in vitro transcription as per instructions in RiboAmp OA kit (Molecular Devices).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin labeling of transcripts was performed in the final round of in vitro transcription using GeneChip IVT Labeling Kit (Affymetrix).
Sample_hyb_protocol	Labeled samples from each timepoint were then hybridized to Affymetrix MOE 430 2.0 expression microarrays according to manufacturer’s protocol and scanned using GeneChip Scanner 3000 (Affymetrix).
Sample_scan_protocol	Labeled samples from each timepoint were then hybridized to Affymetrix MOE 430 2.0 expression microarrays according to manufacturer’s protocol and scanned using GeneChip Scanner 3000 (Affymetrix).
Sample_data_processing	Raw expression signals and present/absent calls were made using Affymetrix GCOS 1.4.
Sample_platform_id	GPL1261
Sample_contact_name	Jacob,,Brown
Sample_contact_institute	NEI
Sample_contact_address	10 Center Drive
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20816
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM327nnn/GSM327856/suppl/GSM327856.CEL.gz
Sample_series_id	GSE13103
Sample_data_row_count	45101

GSM327857

GPL1261

Optic fissure_mouse_E11.5_A

Mouse embryo, optic fissure tissue, embryonic day 11.5

Strain: C57BL/6, Age: Embryonic day 11.5, Tissue: Optic fissure

Expression data were gathered in biological triplicate at E10.5, E11.5 and duplicate at E12.5, each array representing pooled optic fissure tissue from three embryos from a single litter.

Sample_geo_accession	GSM327857
Sample_status	Public on Oct 08 2008
Sample_submission_date	Oct 07 2008
Sample_last_update_date	Oct 07 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Wild type embryos dissected in Rnase free 1X PBS.
Sample_growth_protocol_ch1	A Veritas laser capture microdissection instrument (Molecular Devices) was used to collect optic fissure tissue from frozen sections through staged, wild-type C57BL6/J mouse embryos.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Tissue lysates from three embryos from separate litters and the same embryonic stage were pooled and total RNA was extracted using Absolutely RNA Microprep Kit (Stratagene). Pooled RNA was amplified linearly with two rounds of T7 polymerase-mediated in vitro transcription as per instructions in RiboAmp OA kit (Molecular Devices).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin labeling of transcripts was performed in the final round of in vitro transcription using GeneChip IVT Labeling Kit (Affymetrix).
Sample_hyb_protocol	Labeled samples from each timepoint were then hybridized to Affymetrix MOE 430 2.0 expression microarrays according to manufacturer’s protocol and scanned using GeneChip Scanner 3000 (Affymetrix).
Sample_scan_protocol	Labeled samples from each timepoint were then hybridized to Affymetrix MOE 430 2.0 expression microarrays according to manufacturer’s protocol and scanned using GeneChip Scanner 3000 (Affymetrix).
Sample_data_processing	Raw expression signals and present/absent calls were made using Affymetrix GCOS 1.4.
Sample_platform_id	GPL1261
Sample_contact_name	Jacob,,Brown
Sample_contact_institute	NEI
Sample_contact_address	10 Center Drive
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20816
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM327nnn/GSM327857/suppl/GSM327857.CEL.gz
Sample_series_id	GSE13103
Sample_data_row_count	45101

GSM327858

GPL1261

Optic fissure_mouse_E11.5_B

Mouse embryo, optic fissure tissue, embryonic day 11.5

Strain: C57BL/6, Age: Embryonic day 11.5, Tissue: Optic fissure

Expression data were gathered in biological triplicate at E10.5, E11.5 and duplicate at E12.5, each array representing pooled optic fissure tissue from three embryos from a single litter.

Sample_geo_accession	GSM327858
Sample_status	Public on Oct 08 2008
Sample_submission_date	Oct 07 2008
Sample_last_update_date	Oct 07 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Wild type embryos dissected in Rnase free 1X PBS.
Sample_growth_protocol_ch1	A Veritas laser capture microdissection instrument (Molecular Devices) was used to collect optic fissure tissue from frozen sections through staged, wild-type C57BL6/J mouse embryos.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Tissue lysates from three embryos from separate litters and the same embryonic stage were pooled and total RNA was extracted using Absolutely RNA Microprep Kit (Stratagene). Pooled RNA was amplified linearly with two rounds of T7 polymerase-mediated in vitro transcription as per instructions in RiboAmp OA kit (Molecular Devices).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin labeling of transcripts was performed in the final round of in vitro transcription using GeneChip IVT Labeling Kit (Affymetrix).
Sample_hyb_protocol	Labeled samples from each timepoint were then hybridized to Affymetrix MOE 430 2.0 expression microarrays according to manufacturer’s protocol and scanned using GeneChip Scanner 3000 (Affymetrix).
Sample_scan_protocol	Labeled samples from each timepoint were then hybridized to Affymetrix MOE 430 2.0 expression microarrays according to manufacturer’s protocol and scanned using GeneChip Scanner 3000 (Affymetrix).
Sample_data_processing	Raw expression signals and present/absent calls were made using Affymetrix GCOS 1.4.
Sample_platform_id	GPL1261
Sample_contact_name	Jacob,,Brown
Sample_contact_institute	NEI
Sample_contact_address	10 Center Drive
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20816
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM327nnn/GSM327858/suppl/GSM327858.CEL.gz
Sample_series_id	GSE13103
Sample_data_row_count	45101

GSM327859

GPL1261

Optic fissure_mouse_E11.5_C

Mouse embryo, optic fissure tissue, embryonic day 11.5

Strain: C57BL/6, Age: Embryonic day 11.5, Tissue: Optic fissure

Expression data were gathered in biological triplicate at E10.5, E11.5 and duplicate at E12.5, each array representing pooled optic fissure tissue from three embryos from a single litter.

Sample_geo_accession	GSM327859
Sample_status	Public on Oct 08 2008
Sample_submission_date	Oct 07 2008
Sample_last_update_date	Oct 07 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Wild type embryos dissected in Rnase free 1X PBS.
Sample_growth_protocol_ch1	A Veritas laser capture microdissection instrument (Molecular Devices) was used to collect optic fissure tissue from frozen sections through staged, wild-type C57BL6/J mouse embryos.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Tissue lysates from three embryos from separate litters and the same embryonic stage were pooled and total RNA was extracted using Absolutely RNA Microprep Kit (Stratagene). Pooled RNA was amplified linearly with two rounds of T7 polymerase-mediated in vitro transcription as per instructions in RiboAmp OA kit (Molecular Devices).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin labeling of transcripts was performed in the final round of in vitro transcription using GeneChip IVT Labeling Kit (Affymetrix).
Sample_hyb_protocol	Labeled samples from each timepoint were then hybridized to Affymetrix MOE 430 2.0 expression microarrays according to manufacturer’s protocol and scanned using GeneChip Scanner 3000 (Affymetrix).
Sample_scan_protocol	Labeled samples from each timepoint were then hybridized to Affymetrix MOE 430 2.0 expression microarrays according to manufacturer’s protocol and scanned using GeneChip Scanner 3000 (Affymetrix).
Sample_data_processing	Raw expression signals and present/absent calls were made using Affymetrix GCOS 1.4.
Sample_platform_id	GPL1261
Sample_contact_name	Jacob,,Brown
Sample_contact_institute	NEI
Sample_contact_address	10 Center Drive
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20816
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM327nnn/GSM327859/suppl/GSM327859.CEL.gz
Sample_series_id	GSE13103
Sample_data_row_count	45101

GSM327860

GPL1261

Optic fissure_mouse_E12.5_A

Mouse embryo, optic fissure tissue, embryonic day 12.5

Strain: C57BL/6, Age: Embryonic day 12.5, Tissue: Optic fissure

Expression data were gathered in biological triplicate at E10.5, E11.5 and duplicate at E12.5, each array representing pooled optic fissure tissue from three embryos from a single litter.

Sample_geo_accession	GSM327860
Sample_status	Public on Oct 08 2008
Sample_submission_date	Oct 07 2008
Sample_last_update_date	Oct 07 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Wild type embryos dissected in Rnase free 1X PBS.
Sample_growth_protocol_ch1	A Veritas laser capture microdissection instrument (Molecular Devices) was used to collect optic fissure tissue from frozen sections through staged, wild-type C57BL6/J mouse embryos.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Tissue lysates from three embryos from separate litters and the same embryonic stage were pooled and total RNA was extracted using Absolutely RNA Microprep Kit (Stratagene). Pooled RNA was amplified linearly with two rounds of T7 polymerase-mediated in vitro transcription as per instructions in RiboAmp OA kit (Molecular Devices).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin labeling of transcripts was performed in the final round of in vitro transcription using GeneChip IVT Labeling Kit (Affymetrix).
Sample_hyb_protocol	Labeled samples from each timepoint were then hybridized to Affymetrix MOE 430 2.0 expression microarrays according to manufacturer’s protocol and scanned using GeneChip Scanner 3000 (Affymetrix).
Sample_scan_protocol	Labeled samples from each timepoint were then hybridized to Affymetrix MOE 430 2.0 expression microarrays according to manufacturer’s protocol and scanned using GeneChip Scanner 3000 (Affymetrix).
Sample_data_processing	Raw expression signals and present/absent calls were made using Affymetrix GCOS 1.4.
Sample_platform_id	GPL1261
Sample_contact_name	Jacob,,Brown
Sample_contact_institute	NEI
Sample_contact_address	10 Center Drive
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20816
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM327nnn/GSM327860/suppl/GSM327860.CEL.gz
Sample_series_id	GSE13103
Sample_data_row_count	45101

GSM327861

GPL1261

Optic fissure_mouse_E12.5_B

Mouse embryo, optic fissure tissue, embryonic day 12.5

Strain: C57BL/6, Age: Embryonic day 12.5, Tissue: Optic fissure

Expression data were gathered in biological triplicate at E10.5, E11.5 and duplicate at E12.5, each array representing pooled optic fissure tissue from three embryos from a single litter.

Sample_geo_accession	GSM327861
Sample_status	Public on Oct 08 2008
Sample_submission_date	Oct 07 2008
Sample_last_update_date	Oct 07 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Wild type embryos dissected in Rnase free 1X PBS.
Sample_growth_protocol_ch1	A Veritas laser capture microdissection instrument (Molecular Devices) was used to collect optic fissure tissue from frozen sections through staged, wild-type C57BL6/J mouse embryos.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Tissue lysates from three embryos from separate litters and the same embryonic stage were pooled and total RNA was extracted using Absolutely RNA Microprep Kit (Stratagene). Pooled RNA was amplified linearly with two rounds of T7 polymerase-mediated in vitro transcription as per instructions in RiboAmp OA kit (Molecular Devices).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotin labeling of transcripts was performed in the final round of in vitro transcription using GeneChip IVT Labeling Kit (Affymetrix).
Sample_hyb_protocol	Labeled samples from each timepoint were then hybridized to Affymetrix MOE 430 2.0 expression microarrays according to manufacturer’s protocol and scanned using GeneChip Scanner 3000 (Affymetrix).
Sample_scan_protocol	Labeled samples from each timepoint were then hybridized to Affymetrix MOE 430 2.0 expression microarrays according to manufacturer’s protocol and scanned using GeneChip Scanner 3000 (Affymetrix).
Sample_data_processing	Raw expression signals and present/absent calls were made using Affymetrix GCOS 1.4.
Sample_platform_id	GPL1261
Sample_contact_name	Jacob,,Brown
Sample_contact_institute	NEI
Sample_contact_address	10 Center Drive
Sample_contact_city	Bethesda
Sample_contact_state	MD
Sample_contact_zip/postal_code	20816
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM327nnn/GSM327861/suppl/GSM327861.CEL.gz
Sample_series_id	GSE13103
Sample_data_row_count	45101

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