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Search results for the GEO ID: GSE13130
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Title
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Description
Characteristics
GSM328764
GPL339
LC from biological replicate AhR ko_1 purified Langerhans cells from ear epidermis, isolated after 16 hours of ex vivo cultivation strain C67BL/6 AhR -/- exon2 deleted, 6-8 week old female mice For cell culturing of epidermal cells, an additional digestion with DNAse I (15 min, 130 U/ml) was included. The resulting single cell suspension was cultured in complete medium for 16 hours at a density of 1.5x106 cells/ml in 6-well plates. For sorting, epidermal cells were centrifuged on dense BSA or OptiPrep® (Sigma). The low-density fraction contained 20-35 % LC, which were enriched by FACS sorting of vital MHC-II+ cells using a FACSCalibur (Becton Dickinson, Heidelberg, Germany) to a purity >95 %. Sort purity was verified by re-analysis of samples.
GSM328765
GPL339
LC from biological replicate AhR ko_2 purified Langerhans cells from ear epidermis, isolated after 16 hours of ex vivo cultivation strain C67BL/6 AhR -/- exon2 deleted, 6-8 week old female mice For cell culturing of epidermal cells, an additional digestion with DNAse I (15 min, 130 U/ml) was included. The resulting single cell suspension was cultured in complete medium for 16 hours at a density of 1.5x106 cells/ml in 6-well plates. For sorting, epidermal cells were centrifuged on dense BSA or OptiPrep® (Sigma). The low-density fraction contained 20-35 % LC, which were enriched by FACS sorting of vital MHC-II+ cells using a FACSCalibur (Becton Dickinson, Heidelberg, Germany) to a purity >95 %. Sort purity was verified by re-analysis of samples.
GSM328766
GPL339
LC from biological replicate C57BL/6_1 purified Langerhans cells from ear epidermis, isolated after 16 hours of ex vivo cultivation C57BL/6 mice, 6-8 week old female mice For cell culturing of epidermal cells, an additional digestion with DNAse I (15 min, 130 U/ml) was included. The resulting single cell suspension was cultured in complete medium for 16 hours at a density of 1.5x106 cells/ml in 6-well plates. For sorting, epidermal cells were centrifuged on dense BSA or OptiPrep® (Sigma). The low-density fraction contained 20-35 % LC, which were enriched by FACS sorting of vital MHC-II+ cells using a FACSCalibur (Becton Dickinson, Heidelberg, Germany) to a purity >95 %. Sort purity was verified by re-analysis of samples.
GSM328767
GPL339
LC from biological replicate C57BL/6_2 purified Langerhans cells from ear epidermis, isolated after 16 hours of ex vivo cultivation C57BL/6 mice, 6-8 week old female mice For cell culturing of epidermal cells, an additional digestion with DNAse I (15 min, 130 U/ml) was included. The resulting single cell suspension was cultured in complete medium for 16 hours at a density of 1.5x106 cells/ml in 6-well plates. For sorting, epidermal cells were centrifuged on dense BSA or OptiPrep® (Sigma). The low-density fraction contained 20-35 % LC, which were enriched by FACS sorting of vital MHC-II+ cells using a FACSCalibur (Becton Dickinson, Heidelberg, Germany) to a purity >95 %. Sort purity was verified by re-analysis of samples.
 
 
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