Result

Search results for the GEO ID: GSE13147

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM329257

GPL1261

Myd88-/-Rip2-/-, Lp02, T=4 hours

bone marrow-derived macrophages

Myd88-/-Rip2-/- bone marrow-derived macrophages, Lp02, T=4 hours

Myd88-/-Rip2-/- bone marrow derived macrophages, Lp02, T=4 hours

Sample_geo_accession	GSM329257
Sample_status	Public on Nov 15 2008
Sample_submission_date	Oct 10 2008
Sample_last_update_date	Oct 10 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Macrophages were infected with WT (Lp02) or dotA mutant (Lp03) L. pneumophila for 4 hours at an MOI=25
Sample_growth_protocol_ch1	Macrophages were derived from bone marrow treated with M-CSF for 7 days
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNAbee reagent extraction of total RNA was performed according to manufactuer's protocol. RNA was further purified using the Qiagen RNeasy kit. Quality of total RNA was evaluated by A260/A280 ratio and by electrophoresis on the Agilent Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the recommended Affymetrix protocol from 5 ug total RNA.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station and stained with streptavidin phycoerythrin.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	The data were analyzedusing Affymetrix GeneChip Operating Software (GCOS) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Sunny,,Shin
Sample_contact_email	sunny.shin@yale.edu
Sample_contact_phone	203-737-2409
Sample_contact_laboratory	Roy Lab
Sample_contact_department	Section of Microbial Pathogenesis
Sample_contact_institute	Yale University School of Medicine
Sample_contact_address	295 Congress Ave.
Sample_contact_city	New Haven
Sample_contact_state	CT
Sample_contact_zip/postal_code	06519
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM329nnn/GSM329257/suppl/GSM329257.CEL.gz
Sample_series_id	GSE13147
Sample_data_row_count	45101

GSM329258

GPL1261

Myd88-/-Rip2-/-, Lp03, T=4 hours

bone marrow-derived macrophages

Myd88-/-Rip2-/- bone marrow-derived macrophages, Lp03, T=4 hours

Myd88-/-Rip2-/- bone marrow derived macrophages, Lp03, T=4 hours

Sample_geo_accession	GSM329258
Sample_status	Public on Nov 15 2008
Sample_submission_date	Oct 10 2008
Sample_last_update_date	Oct 10 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Macrophages were infected with WT (Lp02) or dotA mutant (Lp03) L. pneumophila for 4 hours at an MOI=25
Sample_growth_protocol_ch1	Macrophages were derived from bone marrow treated with M-CSF for 7 days
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNAbee reagent extraction of total RNA was performed according to manufactuer's protocol. RNA was further purified using the Qiagen RNeasy kit. Quality of total RNA was evaluated by A260/A280 ratio and by electrophoresis on the Agilent Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the recommended Affymetrix protocol from 5 ug total RNA.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station and stained with streptavidin phycoerythrin.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	The data were analyzedusing Affymetrix GeneChip Operating Software (GCOS) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Sunny,,Shin
Sample_contact_email	sunny.shin@yale.edu
Sample_contact_phone	203-737-2409
Sample_contact_laboratory	Roy Lab
Sample_contact_department	Section of Microbial Pathogenesis
Sample_contact_institute	Yale University School of Medicine
Sample_contact_address	295 Congress Ave.
Sample_contact_city	New Haven
Sample_contact_state	CT
Sample_contact_zip/postal_code	06519
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM329nnn/GSM329258/suppl/GSM329258.CEL.gz
Sample_series_id	GSE13147
Sample_data_row_count	45101

GSM329259

GPL1261

Myd88-/-Trif-/-, Lp02, T=4 hours

bone marrow-derived macrophages

Myd88-/-Trif-/- bone marrow-derived macrophages, Lp02, T=4 hours

Myd88-/-Trif-/- bone marrow derived macrophages, Lp02, T=4 hours

Sample_geo_accession	GSM329259
Sample_status	Public on Nov 15 2008
Sample_submission_date	Oct 10 2008
Sample_last_update_date	Oct 10 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Macrophages were infected with WT (Lp02) or dotA mutant (Lp03) L. pneumophila for 4 hours at an MOI=25
Sample_growth_protocol_ch1	Macrophages were derived from bone marrow treated with M-CSF for 7 days
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNAbee reagent extraction of total RNA was performed according to manufactuer's protocol. RNA was further purified using the Qiagen RNeasy kit. Quality of total RNA was evaluated by A260/A280 ratio and by electrophoresis on the Agilent Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the recommended Affymetrix protocol from 5 ug total RNA.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station and stained with streptavidin phycoerythrin.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	The data were analyzedusing Affymetrix GeneChip Operating Software (GCOS) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Sunny,,Shin
Sample_contact_email	sunny.shin@yale.edu
Sample_contact_phone	203-737-2409
Sample_contact_laboratory	Roy Lab
Sample_contact_department	Section of Microbial Pathogenesis
Sample_contact_institute	Yale University School of Medicine
Sample_contact_address	295 Congress Ave.
Sample_contact_city	New Haven
Sample_contact_state	CT
Sample_contact_zip/postal_code	06519
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM329nnn/GSM329259/suppl/GSM329259.CEL.gz
Sample_series_id	GSE13147
Sample_data_row_count	45101

GSM329260

GPL1261

Myd88-/-Trif-/-, Lp03, T=4 hours

bone marrow-derived macrophages

Myd88-/-Trif-/- bone marrow-derived macrophages, Lp03, T=4 hours

Myd88-/-Trif-/- bone marrow derived macrophages, Lp03, T=4 hours

Sample_geo_accession	GSM329260
Sample_status	Public on Nov 15 2008
Sample_submission_date	Oct 10 2008
Sample_last_update_date	Oct 10 2008
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Macrophages were infected with WT (Lp02) or dotA mutant (Lp03) L. pneumophila for 4 hours at an MOI=25
Sample_growth_protocol_ch1	Macrophages were derived from bone marrow treated with M-CSF for 7 days
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNAbee reagent extraction of total RNA was performed according to manufactuer's protocol. RNA was further purified using the Qiagen RNeasy kit. Quality of total RNA was evaluated by A260/A280 ratio and by electrophoresis on the Agilent Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the recommended Affymetrix protocol from 5 ug total RNA.
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station and stained with streptavidin phycoerythrin.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	The data were analyzedusing Affymetrix GeneChip Operating Software (GCOS) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Sample_platform_id	GPL1261
Sample_contact_name	Sunny,,Shin
Sample_contact_email	sunny.shin@yale.edu
Sample_contact_phone	203-737-2409
Sample_contact_laboratory	Roy Lab
Sample_contact_department	Section of Microbial Pathogenesis
Sample_contact_institute	Yale University School of Medicine
Sample_contact_address	295 Congress Ave.
Sample_contact_city	New Haven
Sample_contact_state	CT
Sample_contact_zip/postal_code	06519
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM329nnn/GSM329260/suppl/GSM329260.CEL.gz
Sample_series_id	GSE13147
Sample_data_row_count	45101

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