Search results for the GEO ID: GSE13173  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM322634 | GPL1261 |
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CTL no cytokine, rep1
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OT-1 CTL no exogenous cytokine
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Cells from OT-1 transgenic mouse (C57BL/6)
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None
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| Sample_geo_accession | GSM322634
| | Sample_status | Public on Feb 01 2009
| | Sample_submission_date | Sep 19 2008
| | Sample_last_update_date | Jan 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Andrey Shaw Washington University School of Medicine
| | Sample_treatment_protocol_ch1 | No exogenous cytokine added
| | Sample_growth_protocol_ch1 | OT-1 splenocytes were cultured with 1mM SIINFEKL peptide for 4 days in IMDM+10%FCS, b-mercaptoethanol, penn/strep. Live cells were harvested using a ficoll gradient and the cells placed back in culture for 24 hours without the addition of exogenous cytokine.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol (Invitrogen) followed by cleanup with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Followed the manufacturer's protocol for the Affymetrix GeneChip 3'IVT express labeling assay.
| | Sample_hyb_protocol | Followed the manufacturer's protocol for the Affymetrix GeneChip 3'IVT express labeling assay.
| | Sample_scan_protocol | GS3000 (Affymetrix); Fluidics Station 450 (Affymetrix)
| | Sample_data_processing | GCOS operating system (Affymetrix); Normalized using a scale factor of 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andrey,,Shaw
| | Sample_contact_email | shaw@pathology.wustl.edu
| | Sample_contact_phone | 314-362-4609
| | Sample_contact_fax | 314-362-8888
| | Sample_contact_laboratory | Andrey Shaw
| | Sample_contact_department | Pathology and Immunology
| | Sample_contact_institute | Washington University in St. Louis
| | Sample_contact_address | 660 S. Euclid Ave.
| | Sample_contact_city | St. Louis
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 63110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM322nnn/GSM322634/suppl/GSM322634.CEL.gz
| | Sample_series_id | GSE13173
| | Sample_data_row_count | 45101
| | |
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GSM322665 | GPL1261 |
|
CTL with IL-12, rep1
|
OT-1 CTL with IL-12
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Cells from OT-1 transgenic mouse (C57BL/6)
|
None
|
| Sample_geo_accession | GSM322665
| | Sample_status | Public on Feb 01 2009
| | Sample_submission_date | Sep 19 2008
| | Sample_last_update_date | Jan 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Andrey Shaw Washington University School of Medicine
| | Sample_treatment_protocol_ch1 | 20ng/ml IL-12
| | Sample_growth_protocol_ch1 | OT-1 splenocytes were cultured with 1mM SIINFEKL peptide for 4 days in IMDM+10%FCS, b-mercaptoethanol, penn/strep. Live cells were harvested using a ficoll gradient and the cells placed back in culture for 24 hours with 20ng/ml rmIL-12 (Invitrogen)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol (Invitrogen) followed by cleanup with Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Followed the manufacturer's protocol for the Affymetrix GeneChip 3'IVT express labeling assay.
| | Sample_hyb_protocol | Followed the manufacturer's protocol for the Affymetrix GeneChip 3'IVT express labeling assay.
| | Sample_scan_protocol | GS3000 (Affymetrix); Fluidics Station 450 (Affymetrix)
| | Sample_data_processing | GCOS operating system (Affymetrix); Normalized using a scale factor of 1500
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andrey,,Shaw
| | Sample_contact_email | shaw@pathology.wustl.edu
| | Sample_contact_phone | 314-362-4609
| | Sample_contact_fax | 314-362-8888
| | Sample_contact_laboratory | Andrey Shaw
| | Sample_contact_department | Pathology and Immunology
| | Sample_contact_institute | Washington University in St. Louis
| | Sample_contact_address | 660 S. Euclid Ave.
| | Sample_contact_city | St. Louis
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 63110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM322nnn/GSM322665/suppl/GSM322665.CEL.gz
| | Sample_series_id | GSE13173
| | Sample_data_row_count | 45101
| | |
|
GSM322667 | GPL1261 |
|
CTL no cyotkine, rep2
|
OT-1 CTL no exogenous cytokine
|
Cells from OT-1 transgenic mouse (C57BL/6)
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None
|
| Sample_geo_accession | GSM322667
| | Sample_status | Public on Feb 01 2009
| | Sample_submission_date | Sep 19 2008
| | Sample_last_update_date | Jan 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Andrey Shaw Washington University School of Medicine
| | Sample_treatment_protocol_ch1 | No exogenous cytokine added
| | Sample_growth_protocol_ch1 | OT-1 splenocytes cultured with 1mM SIINFEKL peptide for 4 days in IMDM+10% FCS with b-mercaptoethanol and penn/strep. Live cells were harvested with a ficoll gradient and put back in culture for 24 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol (Invitrogen) followed by Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Followed the manufacturer's protocol for the Affymetrix GeneChip 3'IVT express labeling assay.
| | Sample_hyb_protocol | Followed the manufacturer's protocol for the Affymetrix GeneChip 3'IVT express labeling assay.
| | Sample_scan_protocol | GS3000 (Affymetrix); Fluidics Station 450 (Affymetrix)
| | Sample_data_processing | GCOS operating system (Affymetrix); Normalized using a scale factor of 1500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andrey,,Shaw
| | Sample_contact_email | shaw@pathology.wustl.edu
| | Sample_contact_phone | 314-362-4609
| | Sample_contact_fax | 314-362-8888
| | Sample_contact_laboratory | Andrey Shaw
| | Sample_contact_department | Pathology and Immunology
| | Sample_contact_institute | Washington University in St. Louis
| | Sample_contact_address | 660 S. Euclid Ave.
| | Sample_contact_city | St. Louis
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 63110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM322nnn/GSM322667/suppl/GSM322667.CEL.gz
| | Sample_series_id | GSE13173
| | Sample_data_row_count | 45101
| | |
|
GSM322668 | GPL1261 |
|
CTL with IL-12, rep2
|
OT-1 CTL with IL-12
|
Cells from OT-1 transgenic mouse (C57BL/6)
|
None
|
| Sample_geo_accession | GSM322668
| | Sample_status | Public on Feb 01 2009
| | Sample_submission_date | Sep 19 2008
| | Sample_last_update_date | Jan 20 2009
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_biomaterial_provider_ch1 | Andrey Shaw Washington University School of Medicine
| | Sample_treatment_protocol_ch1 | 20ng/ml IL-12
| | Sample_growth_protocol_ch1 | OT-1 splenocytes cultured with 1mM SIINFEKL peptide for 4 days in IMDM+10% FCS with b-mercaptoethanol and penn/strep. Live cells were harvested with a ficoll gradient and put back in culture for 24 hours with 20ng/ml rmIL-12 (Invitrogen).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol (Invitrogen) followed by Qiagen RNeasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Followed the manufacturer's protocol for the Affymetrix GeneChip 3'IVT express labeling assay.
| | Sample_hyb_protocol | Followed the manufacturer's protocol for the Affymetrix GeneChip 3'IVT express labeling assay.
| | Sample_scan_protocol | GS3000 (Affymetrix); Fluidics Station 450 (Affymetrix)
| | Sample_data_processing | GCOS operating system (Affymetrix); Normalized using a scale factor of 1500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Andrey,,Shaw
| | Sample_contact_email | shaw@pathology.wustl.edu
| | Sample_contact_phone | 314-362-4609
| | Sample_contact_fax | 314-362-8888
| | Sample_contact_laboratory | Andrey Shaw
| | Sample_contact_department | Pathology and Immunology
| | Sample_contact_institute | Washington University in St. Louis
| | Sample_contact_address | 660 S. Euclid Ave.
| | Sample_contact_city | St. Louis
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 63110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM322nnn/GSM322668/suppl/GSM322668.CEL.gz
| | Sample_series_id | GSE13173
| | Sample_data_row_count | 45101
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